• Title/Summary/Keyword: Zhang Xuecheng

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The Existential Mode and Identity of the Qing Literati - Focusing on Zhang Xuecheng(章學誠) (청대 문인(文人)의 존재 방식과 정체성 - 장학성(章學誠)을 중심으로)

  • 최형섭
    • CHINESE LITERATURE
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    • v.96
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    • pp.145-175
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    • 2018
  • This study was made with the purpose of reconsidering the way of existence of literati(文人), the meaning of writing, and the relationship between cultural power and political power, focusing on Zhang Xuecheng(章學誠, 1738-1801) in the middle of the Qing dynasty. Here, 'Wenren(文人)' was used as a concept equivalent to 'the literary intelligentsia', in other words, 'literati' of English rather than the concept of 'the literary man'. Firstly, Zhang Xuecheng was the person who had lived 64 years of life from Qianlong(乾隆) 3 to Jiaqing(嘉慶) 6, in particular, one of the representatives of the Chinese literati and scholar in the mid and late 18th century. He was born in a typical Gangnam gentry family from Kuaiji(會稽) in Zhejiang province. During adolescence he was grown and educated in hometown Shaoxing(紹興) and Yingcheng(應城) in Hubei province, but Beijing was the city that had the greatest influence on him. After he entered Guozijian(imperial capital academy) at the age of 25 in Qianlong 27(1762), he was mainly active in Beijing and had lived a life of wandering in Zhili(直隸), Henam, Anhui, Hubei province etc. He exchanged ideas with many literati and scholars who led the 18th century. He had many patrons Such as Shen Yefu(沈業富), Ou Yangjin(歐陽瑾), Zhu Fenyuan(朱棻元), Zhu Yun(朱筠), Liang Guozhi(梁國治), Bi Yuan(畢沅), Xie Qikun(謝啓崑) etc, who were prominent government officials and scholars of the time. In spite of passing imperial examinations and becoming the jinshi(進士), he gave up being a bureaucrat, and decided to live as a marginal literati out of office. With the help of his patrons, he solved economic problems mainly through non-regular workers such as tutor, the chief of the local academy, muliao(幕僚, provincial official's adviser) etc. Most of non-official literati in the middle of the Qing dynasty resolved their livelihood problems through irregular jobs like Zhang Xuecheng, while they kept maintaining their identity as intellectuals. Secondly, in intellectual discourses of the 18th century, the academic world was largely divided into moral philosophy(義理), philology(考據), literature(文章, or 詞章). The question of how to define and evaluate the value, relation and status of these three was different according to discussants. However, overall, literary texts were valuated as being less meaningful and worthy than the scholarly texts to deal with moral philosophy and philology. The writings of Zhang Xuecheng generally had the character of a scholar rather than of a literary man, and the meaning and value of his writings could be found in scholarly writings rather than in literary writings. As summarized in the words, "moral philosophy could be proved by philology, literature was the tool to express it," he established the scholarly identity he should seek through the way of integrating moral philosophy and philology centering on the historical writings. Thirdly, including Zhang Xuecheng, Quan Zuwang(全祖望), Yuan Mei(袁枚), Wang Mingcheng(王鳴盛), Zhao Yi(趙翼), Quan Daxin(錢大昕), Yao Nai(姚鼐) etc, represented the Chinese literati and scholars in the 18th century. Coincidentally, they all resigned early and left office, or gave up being official despite passing imperial examinations, and engaged in teaching and writing as marginal literati out of institutional power for a very long time. The backgrounds of their abandonment or early resignation were different, but the ambition and desire to leave the endless works for posterity could be said a common part. In addition, it was necessary to consider that it was a matter of choosing one of the two, in which the 18th century literati could hardly combine official and scholar because of specialized scholarships. It also seemed to be related to the situation that cultural power was becoming a part of the individual choice of Han Chinese literati, for the Manchu regime could not create the leading cultural power.

Optimum Conditions for Transformation of Synechocystis sp. PCC 6803

  • Zang, Xiaonan;Liu, Bin;Liu, Shunmei;Arunakumara, K.K.I.U.;Zhang, Xuecheng
    • Journal of Microbiology
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    • v.45 no.3
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    • pp.241-245
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    • 2007
  • This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher plasmid concentration and longer homologous recombining fragments resulted in a greater number of transformants. For successful transformation, the lowest concentration of plasmid was $0.02\;{\mu}g/ml$, and the shortest homologous recombining fragment was 0.2 kb. Use of Synechocystis sp. PCC 6803 in the logarithmic growth phase resulted in two-fold higher transformation rate than that of the same organism when cells in the latent phase or the plateau phase were used for transformation. Pretreatment of the host strain, Synechocystis sp. PCC 6803, with EDTA (2 mM) for two days prior to transformation increased the transformation efficiency by 23%. Additionally, incubation of the cells and DNA for 5 h under light conditions increased the transformation efficiency by two orders of magnitude. Moreover, recovery treatment of the cells before they were plated onto antibiotic medium also increased the transformation efficiency.

Role of Interleukin-4 (IL-4) in Respiratory Infection and Allergy Caused by Early-Life Chlamydia Infection

  • Li, Shujun;Wang, Lijuan;Zhang, Yulong;Ma, Long;Zhang, Jing;Zu, Jianbing;Wu, Xuecheng
    • Journal of Microbiology and Biotechnology
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    • v.31 no.8
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    • pp.1109-1114
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    • 2021
  • Chlamydia pneumoniae is a type of pathogenic gram-negative bacteria that causes various respiratory tract infections including asthma. Chlamydia species infect humans and cause respiratory infection by rupturing the lining of the respiratory which includes the throat, lungs and windpipe. Meanwhile, the function of interleukin-4 (IL-4) in Ch. pneumoniae respiratory infection and its association with the development of airway hyperresponsiveness (AHR) in adulthood and causing allergic airway disease (AAD) are not understood properly. We therefore investigated the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. In this study, Ch. pneumonia strain was propagated and cultured in HEp-2 cells according to standard protocol and infant C57BL/6 mice around 3-4 weeks old were infected to study the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. We observed that IL-4 is linked with Chlamydia respiratory infection and its absence lowers respiratory infection. IL-4R α2 is also responsible for controlling the IL-4 signaling pathway and averts the progression of infection and inflammation. Furthermore, the IL-4 signaling pathway also influences infection-induced AHR and aids in increasing AAD severity. STAT6 also promotes respiratory infection caused by Ch. pneumoniae and further enhanced its downstream process. Our study concluded that IL-4 is a potential target for preventing infection-induced AHR and severe asthma.

A Novel Esterase from a Marine Metagenomic Library Exhibiting Salt Tolerance Ability

  • Fang, Zeming;Li, Jingjing;Wang, Quan;Fang, Wei;Peng, Hui;Zhang, Xuecheng;Xiao, Yazhong
    • Journal of Microbiology and Biotechnology
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    • v.24 no.6
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    • pp.771-780
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    • 2014
  • A putative lipolytic enzyme gene, named as est9x, was obtained from a marine microbial metagenome of the South China Sea. Sequence analysis showed that Est9X shares lower than 27% sequence identities with the characterized lipolytic enzymes, but possesses a catalytic triad highly conserved in lipolytic enzymes of the ${\alpha}/{\beta}$ hydrolase superfamily. By phylogenetic tree construction, Est9X was grouped into a new lipase/esterase family. To understand Est9X protein in depth, it was recombinantly expressed, purified, and biochemically characterized. Within potential hydrolytic activities, only lipase/esterase activity was detected for Est9X, confirming its identity as a lipolytic enzyme. When using p-nitrophenol esters with varying lengths of fatty acid as substrates, Est9X exhibited the highest activity to the C2 substrate, indicating it is an esterase. The optimal activity of Est9X occurred at a temperature of $65^{\cric}C$, and Est9X was pretty stable below the optimum temperature. Distinguished from other salt-tolerant esterases, Est9X's activity was tolerant to and even promoted by as high as 4 M NaCl. Our results imply that Est9X is a unique esterase and could be a potential candidate for industrial application under extreme conditions.

Cloning and Characterization of a ${\beta}$-Glucosidase from Marine Microbial Metagenome with Excellent Glucose Tolerance

  • Fang, Zemin;Fang, Wei;Liu, Juanjuan;Hong, Yuzhi;Peng, Hui;Zhang, Xuecheng;Sun, Baolin;Xiao, Yazhong
    • Journal of Microbiology and Biotechnology
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    • v.20 no.9
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    • pp.1351-1358
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    • 2010
  • The demand for ${\beta}$-glucosidases insensitive to product inhibition is increasing in modern biotechnology, for these enzymes would improve the process of saccharification of lignocellulosic materials. In this study, a ${\beta}$-glucosidase gene that encodes a 442-amino-acid protein was isolated from a marine microbial metagenomic library by functional screening and named as bgl1A. The protein was identified to be a member of the glycoside hydrolases 1 family, and was recombinantly expressed, purified, and biochemically characterized. The recombinant ${\beta}$-glucosidase, Bgl1A, exhibited a high level of stability in the presence of various cations and high concentrations of NaCl. Interestingly, it was activated by glucose at concentrations lower than 400 mM. With glucose further increasing, the enzyme activity of Bgl1A was gradually inhibited, but remained 50% of the original value in even as high as 1,000 mM glucose. These findings indicate that Bgl1A might be a potent candidate for industrial applications.

PspAG97A: A Halophilic α-Glucoside Hydrolase with Wide Substrate Specificity from Glycoside Hydrolase Family 97

  • Li, Wei;Fan, Han;He, Chao;Zhang, Xuecheng;Wang, Xiaotang;Yuan, Jing;Fang, Zemin;Fang, Wei;Xiao, Yazhong
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1933-1942
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    • 2016
  • A novel ${\alpha}-glucoside$ hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized ${\alpha}-glucoside$ BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only ${\alpha}-1,4-glucosidic$ linkage, but also ${\alpha}-1,6-$ and ${\alpha}-1,2-glucosidic$ linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with ${\alpha}-1,6-linkage$. These characteristics are clearly different from other known ${\alpha}-glucoside$ hydrolases in GH97, implying that PspAG97A is a unique ${\alpha}-glucoside$ hydrolase of GH97.

Ultrastructural changes of Haematococcus pluvialis (Chlorophyta) in process of astaxanthin accumulation and cell damage under condition of high light with acetate

  • He, Bangxiang;Hou, Lulu;Zhang, Feng;Cong, Xiaomei;Wang, Zhendong;Guo, Yalin;Shi, Jiawei;Jiang, Ming;Zhang, Xuecheng;Zang, Xiaonan
    • ALGAE
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    • v.35 no.3
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    • pp.253-262
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    • 2020
  • Haematococcus pluvialis is a commercial microalga that can produce high quantities of astaxanthin. Under induced conditions, some important changes in the subcellular structures related to astaxanthin accumulation were observable. For example, a large number of astaxanthin granules, oil structures and starch granules appeared in the thick-walled cells; Astaxanthin granules gradually dissolved into the oil structures and spread throughout the entire cell with the fusion and diffusion process of oil structures during the middle and late stages of induction; The plastoglobules were closed to the newly formed structures, and some plastoglobules would abnormally increase in size under stress. Based on observations of cell damage, the degradation of membrane structures, such as chloroplasts, was found to be the primary form of damage during the early stage of induction. During the middle stage of induction, some transparent holes were exposed in the dissolving astaxanthin granules in the cytoplasm. In thick-walled cells, these transparent holes were covered by oil substances dissolving astaxanthin, thereby avoiding further damage to cells. Given the relatively few oil structures, in non-thick-walled cells, the transparent holes expanded to form multiple transparent areas, eventually resulting in the rupture and death of cells. These results suggested that the high level of synthesis and the wide range diffusion of oil explained the expansion of astaxanthin in H. pluvialis.