• Korean Circulation Journal
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• 제53권1호
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• pp.34-46
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• 2023

Background and Objectives: Diabetes mellitus (DM)-associated heart failure (HF) causes high morbidity and mortality. In this study, we established a zebrafish larvae model for in vivo research on diabetic HF. Methods: DM-like phenotypes were induced by treating zebrafish larvae with a combination of D-glucose (GLU) and streptozotocin (STZ). HF was induced by treatment with terfenadine (TER), a potassium channel blocker. Additionally, myocardial contractility, motility, and viability were evaluated. Results: The zebrafish larvae treated with a combination of GLU and STZ showed significantly higher whole-body glucose concentrations, lower insulin levels, and higher phosphoenolpyruvate carboxykinase levels, which are markers of abnormal glucose homeostasis, than the group treated with only GLU, with no effect on viability. When treated with TER, DM zebrafish showed significantly less myocardial fractional shortening and more irregular contractions than the non-DM zebrafish. Furthermore, in DM-HF with reduced ejection fraction (rEF) zebrafish, a significant increase in the levels of natriuretic peptide B, a HF biomarker, markedly reduced motility, and reduced survival rates were observed. Conclusions: We established a DM-HFrEF zebrafish model by sequentially treating zebrafish larvae with GLU, STZ, and TER. Our findings indicate the potential utility of the developed zebrafish larvae model not only in screening studies of new drug candidates for DM-HFrEF but also in mechanistic studies to understand the pathophysiology of DM-HFrEF.

• 한국응용과학기술학회지
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• 제39권3호
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• pp.442-453
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• 2022

다양한 동물 모델이 인간 질병, 의약품의 효능 및 작용 메커니즘을 연구하는 데 사용되고 있다. Zebrafish(Danio rerio)는 여러 가지 장점이 있어 인간 질병에 대한 중개 연구의 모델로 점점 더 폭넓게 활용되고 있다. 본 논문은 Pubmed, Google Scholar, Scopus에서 2020년 12월까지 최근 10년간 zebrafish 모델, 천연물(한약), in vivo 스크리닝의 키워드를 사용하여 저널에 게재된 논문을 검토하여 필요한 정보를 얻었다. 이 리뷰에서 우리는 천연물(한약) 연구에 대한 다양한 제브라피쉬 질병 모델의 최근 경향에 대해 논의하였다. 특히, 암, 안질환, 혈관 질환, 당뇨병 및 합병증, 피부질환에 중점을 두었고, zebrafish 배아를 사용하여 이들 질병에 대한 의약품의 분자 작용 메커니즘에 관해 언급하였다. Zebrafish는 실험실에서 임상 연구까지의 격차를 줄이는 데 중추적 역할을 할 수 있는 중요한 동물 모델이다. Zebrafish는 의약품이나 화장품 개발, 질병의 병인론을 이해하기 위해 사용되고, 이로 인해 생의학 연구에서 설치류의 사용을 줄이는 데 크게 기여하고 있다.

• 통합자연과학논문집
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• 제14권4호
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• pp.155-158
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• 2021

To study the effects of hormones and neurotransmitters, zebrafish (Danio rerio) are a great substitute for water fleas (Daphnia). The zebrafish is an ideal vertebrate model because it has a transparent embryonic stage. It is easy to get consistent heart rate measurements in embryonic zebrafish when treating them with hormones and neurotransmitters. To observe the heart rate, two to three embryonic zebrafish are anesthetized with MS-222 and then transferred to a glass slide specifically designed for heart observation and easy application of various chemicals. After the heartbeats are counted for 2 minutes, apply either 100 µM epinephrine or 100 µM acetylcholine to the zebrafish. Wait 5, 10, and 20 minutes and count the heartbeats at each time point. All procedures are repeated three times. The final results are averaged and analyzed by using statistical methods. The above method which we have developed is practical enough for high school students to measure the heart rate in zebrafish under various conditions and to analyze the data set.

• Animal cells and systems
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• 제7권4호
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• pp.303-307
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• 2003

The aim of this study is to investigate whether alcohol alters learning and memory processes pertaining to emotional and spatial factors using the active avoidance and T-maze task in zebrafish. In the active avoidance task, zebrafish were trained to escape from one compartment to another to avoid electric shocks (unconditioned stimulus) following a conditioned light signal. Acquisition of active avoidance task appeared to be normal in zebrafish that were treated with 1% alcohol for 30 min for 17 days until the end of the behavioral test, and retention ability of learned behavior, tested 2 days later, was the same as control group. In the T-maze task, the time to find a reservoir was compared. While the latency was similar during the 1 st training session between control and alcohol-treated zebrafish, it was significantly longer in alcohol-treated zebrafish during retention test 24 h later. Furthermore, when alcohol was treated 30 min after 2nd session without prior treatment, zebrafish demonstrated similar retention ability compared to control. These results suggest that chronic alcohol treatment alters spatial learning of zebrafish, but not emotional learning.

• Fisheries and Aquatic Sciences
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• 제25권3호
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• pp.117-139
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• 2022

Synucleinopathies such as Parkinson's disease (PD) are incurable neurodegenerative conditions characterised by the abnormal aggregation of α-synuclein protein in neuronal cells. In PD, fibrillary synuclein aggregation forms Lewy bodies and Lewy neurites in the substantia nigra and cortex on the brain. Dementia with Lewy bodies and multiple system atrophy are also associated with α-synuclein protein abnormalities. α-synuclein is one of three synuclein proteins, and while its precise function is still unknown, one hypothesis posits that α-synuclein propagates from the enteric nervous system through the vagus nerve and into the brain, resulting in synucleinopathy. Studies on synucleinopathies should thus encompass not only the central nervous system but must necessarily include the gut and microbiome. The zebrafish (Danio rerio) is a well-established model for human neuronal pathologies and have been used in studies ranging from genetic models of hereditary disorders to neurotoxin-induced neurodegeneration as well as gut-brain-axis studies. There is significant genetic homology between zebrafish and mammalian vertebrates which is what makes the zebrafish so amenable to modelling human conditions but in the case of synucleinopathies, the zebrafish notably does not possess an α-synuclein homolog. Synuclein orthologs are present in the zebrafish however, and transgenic zebrafish that carry human α-synuclein have been generated. In addition, the zebrafish is a highly advantageous model and ideal replacement for reducing the use of mammalian models. This review discusses the application of the zebrafish as a model for synucleinopathies in efforts to further understand synuclein function and explore therapeutic strategies.

• 한국양식학회지
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• 제10권4호
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• pp.395-402
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• 1997

The study has been conducted in order to understand the inheritance of body color in the wild type zebrafish (zebra danio), Danio rerio, and its golden mutant (golden danio). The body color was also studied to determine the effect of golden coloration on the survival rate of zebrafish eggs and larvae up to 15 days after fertilization. Reciprocal monohybrid crosses between the wild and the golden type of zebrafish indicated that golden coloration was controlled by a single gene which had two alleles. Transmission of these alleles from parents to their progenies followed the principles of dominance and segregation based on Mendelian inheritance. Similar results from the reciprocal crosses implied that a locus for golden coloration was located on an autosomal chromosome. On the other hand, average survival rates from four different types of mating between, and within, zebra and golden danio suggested that golden coloration seemed to be associated with the survival rate of zebrafish, especially in its early embryonic stage. This indicated that homozygous recessive golden mutation was likely to weaken the golden danio's chance of survival.

• 한국자기공명학회논문지
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• 제20권1호
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• pp.13-16
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• 2016

Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.

• 한국동물생명공학회지
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• 제37권3호
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• pp.176-182
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• 2022

Norflurazon is widely used on agricultural lands and has a high potential to pollute water sources. However, its effects on fish have not been fully elucidated. The purpose of our study was to determine whether norflurazon adversely affects the developmental stage of zebrafish, which are frequently used as a model system to evaluate the environmental impact of pollutants. Norflurazon interfered with the hatching of zebrafish embryos and induced several sublethal deformities including body length reduction, increased yolk sac volume, and enlargement of the pericardial region. We further examined the cardiotoxicity of norflurazon in the flk1:eGFP transgenic zebrafish line. The vascular network, mainly in the brain region, was significantly disrupted in norflurazon-exposed zebrafish. In addition, due to the failure of cardiac looping, norflurazon-exposed zebrafish had an abnormal cardiac structure. These developmental abnormalities were related to the apoptotic process triggered by norflurazon. Overall, the present study demonstrated the non-target toxicity of norflurazon by analyzing the hazardous effects of norflurazon on developing zebrafish.

• 한국발생생물학회지:발생과생식
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• 제15권3호
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• pp.215-221
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• 2011

Endocytosis of the Notch ligand, DeltaD, by mind bomb1 is indispensable for activation of Notch in cell fate determination, proliferation, and differentiation during zebrafish neurogenesis. Loss of mind bomb1 activity as an E3 Ubiquitin ligase causes the accumulation of deltaD at the plasma membrane and results in the ectopic neurogenic phenotype by activation of Notch in early zebrafish embryogenesis. However, the regulatory mechanism of deltaD during neurogenesis is not identified yet. This study aims to analyze the pathway of mib1 and deltaD after endocytosis in vivo during zebrafish embryogenesis. Mind bomb1 and deltaD are co-localized into autophagosome and mutant form of mind bomb1 fails to cargo deltaD into autophagosomes. These findings suggest that mind bomb I mediates deltaD regulation by autophagy in an ubiquitin-dependent manner during zebrafish embryogenesis.

• BMB Reports
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• 제45권10호
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• pp.565-570
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• 2012

We recently reported that a water extract of laurel or turmeric, 1,8-cineole enriched fractions, showed hypolipidemic activity in the zebrafish model. Therefore, the present study investigated the cineole's anti-oxidant and anti-inflammatory activities in lipoprotein metabolism in vitro and in vivo. Cineole had inhibitory effects on cupric ion-mediated oxidation of lipoproteins in general, while simultaneously enhancing ferric ion removal ability in high-density lipoprotein (HDL). Hypercholesterolemia was induced in zebrafish using cholesterol-feeding treatment, 4% cholesterol, for 3 weeks. After feeding with or without the addition of cineole, the results revealed that cineole possessed lipid-lowering and anti-inflammatory activities in hypercholesterolemic zebrafish. In addition, serum amyloid A and interleukin-6 levels were lowered and lipid accumulation was decreased in the liver. Conclusively, 1,8-cineole was found to have anti-oxidant activities in lipoprotein metabolism both in vitro and in vivo with simultaneous reduction of lipid accumulation in the liver of zebrafish.