• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.555-563
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• 2016

The morphological characteristics and genetic relationships among 32 germplasms of Zanthoxylum schinifolium and Zanthoxylum piperitum collected from two farms in Korea were investigated. The traits with the most variability were seed color, leaf size, and spine size. The intraspecific polymorphism of Z. schinifolium and Z. piperitum was 96.5% and 60.3%, respectively. The genetic diversity and Shannon’s information index values ranged from 0.11 to 0.33 and 0.19 to 0.50, with average values of 0.26 and 0.42, respectively. Two ISSR primers (UBC861 and UBC862) were able to distinguish the different species. The genetic similarity matrix (GSM) revealed variability among the accessions ranging from 0.116 to 0.816. The intraspecific GSM for Z. schinifolium and Z. piperitum was 0.177-0.780 and 0.250-0.816, respectively. The GSM findings indicate that Z. schinifolium and Z. piperitum accessions have high genetic diversity and possess germplasms qualifying as good genetic resources for cross breeding. The clustering analysis separated Z. schinifolium and Z. piperitum into independent groups, and all accessions could be classified into three categories. Z. Schinifolium var. nermis belonged to independent groups. Comparison of the clusters based on morphological analysis with those based on ISSR data resulted in an unclear pattern of division among the accessions. The study findings indicate that Z. schinifolium and Z. piperitum accessions have genetic diversity, and ISSR markers were useful for identifying Z. schinifolium and Z. piperitum.

• Journal of Life Science
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• v.18 no.2
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• pp.167-174
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• 2008

Antimicrobial activity of Zanthoxylum piperitum was investigated against Streptococcus mutans that causes dental caries. Although the methylene chloride extract of seeds exhibited higher antimicrobial activity than other organic solvent extracts, including methanol, ethyl acetate, and hexane extracts of pericarps or seeds of Z. piperitum, essential oils prepared from both seeds and pericarps possessed more potent inhibitory activity than the methylene chloride extract of seeds. The minimal inhibitory concentrations (MICs) of the essential oils of seeds and pericarps were 0.3 mg/ml and 4.0 mg/ml against S. mutans, respectively. When the seed essential oil was further separated into seven fractions (CS-SD-A${\sim}$CS-SD-G) by thin layer chromatography (TLC), all fractions exhibited lower antimicrobial activity than the essential oil. To understand the antimicrobial ingredients of Z. piperitum, seeds the gas chromatography-mass spectrometry (GC-MS) data of the methylene chloride extract of seeds was compared with those of the seed essential oil (CS-SD). Whereas the methylene chloride extract of seeds contained carvacrol (0.24%), ${\beta}$-caryophyllene (1.72%), and ${\alpha}$-humulene (0.88%), which were previously known to inhibit growth of S. mutans, the seed essential oil contained sabinene (1.57%), linalool (1.55%), citronellal (13.67%), terpinene-4-ol (0.45%), citronellol (3.69%), geraniol (0.9%), linalyl acetate (1.35%), ${\beta}$-caryophyllene (1.35%), ${\alpha}$-humulene (0.78%), and ${\delta}$-cadinene (0.67%) in this regard. These results indicate that Z. piperitum seeds possess various inhibitory substances against S. mutans, and an effective method to isolate the active ingredients from the seeds is to prepare the essential oil. These results also suggest that the essential oil of Z. piperitium seeds may be applicable to preventing dental caries.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.943-951
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• 1997

The present study was undertaken to investigate the inhibition effects of sancho(Zanthoxylum schinifolium) on lipid peroxidation and liver damage in carbon tetrachloride tetracloride-treated mice. Mice aged 3 weeks old were fed diets containing either dry sancho powder, methanol extract or hyperoside isolated from sancho at the various levels for 2 weeks(sancho, methanol extract from sancho) or 3 days (hyperoside) before $CCl_{4}$ treatments. Seed coats of sancho added to diet with the levels from 1.25 to 5.0% significantly reduced(p<0.001) thiobarbituric acid reactive substances(TBARS) formation in liver and serum of mice treated with carbon tetrachloride. $CCl_{4}$ treatments significantly increased (p<0.001) in liver TBARS comparing with those of the untreated control, while methanol extract from the seed coat of sancho added to diet with the levels from 0.5 to 1.0% significantly reduced (p<0.01) to 20mg per kg of body weight showed significant reduction(p<0.01, p<0.001) of liver TBARS in mice treated with $CCl_{4}$. $CCl_{4}$ treatments significantly increased(p<0.05) in serum TBARS comparing with those the levels from 0.5 to 1.0% significantly reduced(p<0.01) serum TBARS in mice treated with $CCl_{4}$, and administration of hyperoside with the levels from 10 to 20mg per kg of body weight showed significant reduction(p<0.01, p<0.001) of serum TBARS in mice treated with $CCl_{4}$. $CCl_{4}$ significantly increased(p<0.001) in serum AST activities comparing with those of the untreated control, while either addition(0.5, 1.0%) of methanol extract from sancho or administration(10, 20mg/kg) of hyperoside significantly reduced(p<0.001, p<0.05) serum AST activities in $CCl_{4}$-treated mice. $CCl_{4}$ treatments showed significant increases(p<0.001) in serum ALT activities comparing with those of the untreated control, while either addition(0.5, 1.0%) of methanol extract from sancho or administration (10, 20mg/kg) of hyperoside showed moderate decreases(p<0.01, p<0.001) serum ALT activities in $CCl_{4}$-treated mice.

• Korean Journal of Medicinal Crop Science
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• v.28 no.6
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• pp.421-427
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• 2020

Morus alba, Anti-obesity, C57BL/6 Mice, Expression, Flavonoid, Gene, Mulberry Background: The seed oil of Zanthoxylum schinifolium S. et Z. (sancho) is a traditional cooking oil that has long been sold at a very high price however, depending on the method of extraction and storage, this oil becomes rancid occurs very quickly. Therefore, this study aimed to find a material that prevents rancidity and improves the storage properties of sancho oil. Methods and Results: Sancho oil was extracted using an extraction press, and acid values were compared with commercially available vegetable oils, sancho oil had a higher acid value than other vegetable oils. A very high acid value was observed in sancho oil stored for 6 months, regardless of temperature, requiring an effective storage method. The high acid value and the decrease in turbidity of sancho oil are dependent on the days of sedimentation. Treatment with sodium bicarbonate by concentration resulted in minimal changes in acid value over time. However, minor differences were detected among the treatment concentrations. Ascorbic acid was added to maximize the effect of sodium bicarbonate, and it was observed that ascorbic acid did not improve the antioxidant effect. The sodium bicarbonate and ascorbic acid mixture resulted in minimal change in acid value at temperature up to 25℃. Conclusions: Sancho oil becomes rancid very quicky and requires efficient storage techniques. Sodium bicarbonate and ascorbic acid have been proven to be useful as safe anti-racidity agents without causing harm to humans.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.183-189
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• 2004

This study was conducted to examine the changes in the seed physical characters and germinative properties of three tree species at three different storage times in order to determine the most suitable storage time and condition. Seed physical characters and germinative properties were examined from seeds of Fraxinus rhynchophylla, Zanthoxylum schinifolium and Staphylea bumalda that were stored at -18C for 1, 3 and 10 years, and the growth performance and physiological characters of their seedlings were analyzed. Seed physical characters and germinative properties showed significant differences between three storage times as well as three tree species. Seed moisture content of F rhynchophylla and S. bumalda and seed fresh weight of Z. schinifolium decreased with increase of storage time. Storage time represented negative correlation with moisture content and fresh weight (r = -0.822, P<0.01). Seed percent germination of Z. schinifolium and S. bumalda stored for 3 and 10 years decreased less than 15% and 10.7% respectively. Mean germination times for seed of F. rhynchophylla, Z. schinifolium and S. bumalda were delayed 11.9, 5.7 and 9.7 days after a storage time of 10 years, respectively. However the growth and viability of seedlings didn't show a special pattern for storage time and tree species.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.87-96
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• 1995

We examined the effects of seed treatment on field germination rate and seedling growth for four woody species, Euonymus alatus, Thea sinensis, Zanthoxylum piperitum, Nandina domestia which are economically useful in Korea. The seeds of each species were purified carefully after collection during Oct. and Nov.. E. alatus, T. sinensis, and Z. piperitum were sowed in the experimental field after stored in open ground with treatment of Pon-Pon. $H_2O_2$, $GA_3$, scarification. and N. domestica was sowed in container within green house after treatment of three different periods(7, 15, 21days) in growth chamber(4, 25, $32^{\circ}C$). Germination rate and seedling growth were measured in mid-Oct.. In E. alatus germination rate was higher in storaging in open ground after soaking in $GA_3$(67.1%) than in only storaging in open ground(18.4%). But the rate in T. sinensis showed no differences between storage in open ground after treatment of $GA_3$, and scarification, and only storage in open ground, Germination rate of Z. piperitum was much higher in storaging in open ground after treatment of Pon-Pon(80.3%) than in only storaging in open ground(12.4%). In N. domestica. seeds stored for 7 days at $32^{\circ}C$ were germinated faster than those stored for 7 days at $4^{\circ}C$, and germination rate of the former was also higher than that of the latter. Peak Times of seed germination in field were order of E. alatus (32 days). Z. piperitum (49 days). T. sinensis (83 days), N. domestica (87 days). The growth of seedling germinated in field showed a good result because of early germination following the effect of seed treatment. The periods of highest growth performances of the seedlings were 72 days in late Jun.-mid Jul. in E. alatus. 59 days in late Jun.- mid Jul. in Z. Piperitum, and 45 days in mid Aug.- mid Sep. in T. sinensis.

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.25-34
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• 2018

This study was performed to discover the comparison of the understory species composition and the growth velocity of the regeneration seedlings in Pinus densiflora forests being managed by the seed-tree method from 2008 year. The investigation site located in Bonghwa, Gyeongsangbuk-do province was established by eighteen quadrats such as six control ones, six slope ones and 6 concave ones, in which we carried out the vegetation survey and measured annual height growth of each seedlings. As a result of vegetation analysis, the understory vegetation of the seed-tree forest area was mainly composed of shrub plants such as Lespedeza bicolor, Zanthoxylum schinifolium, Securinega suffruticosa, Lespedeza maximowiczii and Stephanandra incisa, herb plants such as Miscanthus sinensis var. purpurascens, Carex humilis var. nana, Spodiopogon sibiricus and Artemisia keiskeana, and vine plants such as Pueraria lobata, Smilax sieboldii, Dioscorea batatas, Actinidia arguta, Vitis amurensis and Rubus crataegifolius. Especially, the vine plants were relatively more imported to the concave site than the control site and the slope site. As a result of measurement of growth velocity, the seedlings of the Pinus densiflora appeared to be 3,175 trees/ha for the non-suppressed trees and 7,842 trees/ha for the suppressed trees. In cases of the concave site, individuals of seedlings were much lower than those on the slope site, probably due to the competition effects of vine plants. Consideringly, silvicultural practices for clearing the vine plants should be inevitably accompanied. Also, the growth velocity of the suppressed and non-suppressed trees has been constantly increasing, those of the non-suppressed trees much higher than the suppressed trees. This represents that the seedlings grow rapidly after the suppressed period with competition to the surrounding plants. Therefore, specific practices such as blush control and vine clear should be carried out at the beginning stage of regeneration in the seed-tree stand.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.227-234
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• 1993

This study was carried out to seek for a most effective hastening method of seed germination by Pon-Pon treatment and asexual multiplication method by cutting in Zanthozylum piperitum. The results obtained in this study were as follows : 1. Cold moist stratification under the ground accompanied with Pon-Pon treatment was shown 74.3% of seed germination, which was the most effective one among pretreatment methods with significance at 1% level. 2. Height growth was shown continuous growth pattern and the maximum growth was shown during the period 1 July to 31 July, which corresponds 40.3% of total annual growth. 3. Although no noticeable difference among the kind of plant growth regulator in the rooting rate, there was difference among concentrations with significance at 1% level. 4. In the case of number of primary roots and T/R rate of rooted cuttings, treatment of plant growth regulators was significantly superior to control. 5. T/R rate in rooted cuttings was a high negative correlation (r=-0.7164) with the number of primary roots.

• The Korean Journal of Pesticide Science
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• v.7 no.4
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• pp.248-257
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• 2003

The objective of this experiment was to search plant species with herbicidal activity in Korea. Two hundred native plants were collected and their methanol extracts were obtained. Herbicidal activity of methanol extracts were determined by seed bioassay using canola (Brassica napus L.) seedlings. Six plants such as Staphylea bumalda, Wistaria floribunda, Allium victorialis, Rumex crispus, Chionanthus retusa, and Ulmus parvifolia were highly herbicidal: their $GR_{50}$ values were < $1,000{\mu}g\;g^{-1}$. In addition, seventeen plants such as Galium spurium, Zelkova serrata, Campsis grandiflora, Eucommia ulmoides, Sorbus commixta, Deutzia glabrata, Cercis chinensis, Alnus hirsuta, Zanthoxylum schinifolium, Quercus acutissima, Robinia pseudoacacia, Gleditsia japonica, Kerria japonica, Ligustrum obtusifolium, Thuja orientalis, Chamaecyparis obtusa, and Pulsatilla koreana showed herbicidal activity: their $GR_{50}$ values were between 1,000 and $2,000{\mu}g\;g^{-1}$. However, 177 plants showed no herbicidal activity. Plants with herbicidal activity found in this study could be used for weed management and herbicidal compounds in such herbicidal plants could be used as lead compounds in the development of new herbicides.

• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.455-463
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• 2018

Background: Sancho (Zanthoxylum schinifolium Siebold and Zucc) oil is used as a traditional medicinal material to treat severs stomach inflammation and as a diuretic. This study was carried out to investigate the effect of addition of antioxidants and blended oil the storage stability and safety of the biomaterial. Methods and Results: The effects of temperature and light on sancho oil were investigated, and the ability of antioxidants in preventing rancidity of the oil was discovered. Under fluorescent light and in darkness, the acidity of the oil was much lower than that under direct sunlight. The addition of antioxidants decreased the acid value of sancho oil; the antioxidant that showed the best results in this regard was 0.5% propolis. The acid value of canola oil, which had the lowest acid value compared with that of other oils, and blended oil, containing 5% canola oil in sancho oil, decreased by 5.5% and 15%, respectively. About one acid value decrease was observed for every 1% increase in blending with canola oil. As the concentration of canola oil increased, the viscosity and the elightness (L valu) of sancho oil increased slightly, while the blueness (b value) decreased. Conclusions: The results of this study may contribute to ensuring food safety during preservation and the industrialization of the presevation of sancho oil.