• Title/Summary/Keyword: Yun Dong-ju

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Plant regeneration through multiple-shoot induction and ex vitro rooting in Vaccinium oldhamii Miq. (정금나무(Vaccinium oldhamii Miq.)의 다신초 유도 및 기외발근을 통한 식물체 재분화)

  • Yun, Ayoung;Kim, Tae Dong;Kim, Ji Ah;Lee, Na Nyum;Cheong, Eun Ju;Kim, Yong Wook
    • Journal of Plant Biotechnology
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    • v.49 no.1
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    • pp.82-89
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    • 2022
  • In vitro techniques were developed for propagating Vaccinium oldhamii using shoots with apical buds. Explants having an apical bud were cultured on Murashige and Skoog (MS) medium supplemented with 1.0, 2.0, and 5.0 mg/L of each zeatin, thidiazuron, 6-benzylaminopurine (BA), and 6-(γ,γ-dimethylallylamino)purine (2-iP) in order to induce multiple shoots. Among the tested treatments, the 2.0 mg/L of 2-iP proved to be most suited for the multiplication and growth of shoots; the multiple shoot induction rate was 100.0%, the average number of shoots was 7.4 per explant, and the average shoot length was 51.7 mm. The in vitro elongated shoots were rooted on half-strength MS medium containing various concentrations of indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA). However, overall callus overgrowth was observed in all treatments and resulted in necrosis and abnormal shoot growth in root formation. A low concentration (0.5 mg/L) of IBA was appropriate for normal root development and the in vitro rooting rate was 30%. Ex vitro treatments on root formation using various concentrations of IBA with Talc powder and two types of rooting substrates (Flexi-Plugs or Horticultural soil) were examined. The ex vitro rooting rate (80%) and length of roots (32.9 mm) were obtained when the cut ends of the shoots were treated with 1.0 mg/L IBA and cultivated in Horticultural soil for 2 months. These findings suggest that ex vitro rooting is the more effective method for improving root formation in Vaccinium oldhamii than in vitro rooting.

Effects of different Bacillus licheniformis and Bacillus subtilis ratios on nutrient digestibility, fecal microflora, and gas emissions of growing pigs

  • Kim, Yong Ju;Cho, Sung Bo;Song, Min Ho;Lee, Sung Il;Hong, Seok Man;Yun, Won;Lee, Ji Hwan;Oh, Han Jin;Chang, Se Yeon;An, Jae Woo;Go, Young Bin;Song, Dong Cheol;Cho, Hyun Ah;Kim, Hyeun Bum;Cho, Jin Ho
    • Journal of Animal Science and Technology
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    • v.64 no.2
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    • pp.291-301
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    • 2022
  • The objective of this study was to evaluate the effects of different mixing ratios of Bacillus licheniformis and Bacillus subtilis in diets on nutrient digestibility, fecal microflora, and odor gas emissions of growing pigs. A total of four crossbred ([Landrace × Yorkshire] × Duroc) barrows with average body weight (BW) of 41.2 ± 0.7 kg were randomly allotted four diets over four periods in a 4 × 4 Latin square design. Treatments were as follows: Control (CON, basal diet), CON + 0.2% probiotic complex (L4S6, B. licheniformis and B. subtilis at a 4:6 ratio), CON + 0.2% probiotic complex (L5S5, B. licheniformis and B. subtilis at a 5:5 ratio), CON + 0.2% probiotic complex (L6S4, B. licheniformis and B. subtilis at a 6:4 ratio). Dietary probiotic supplementation showed higher crude protein (CP) digestibility values and lower Escherichia coli counts in fecal samples than the CON group (p < 0.05). There was no significant difference in NH3 or H2S emission until day 3. The positive effect of H2S and NH3 emissions was detected earlier with the L4S6 and L5S5 compared to the L6S4, which had a lower ratio of B. subtilis. Both the L4S6 and L5S5 probiotic complexes significantly decreased the fecal H2S and NH3 emission in days 4 and 6 (p < 0.05). On day 7, all probiotic complexes decreased (p < 0.05) H2S and NH3 emissions than the CON group. Our results agreed that the dietary supplementation of Bacillus licheniformis and Bacillus subtilis complexes in growing pigs can significantly improve CP digestibility and reduce fecal E. coli counts, NH3 and H2S emissions. Notably, the higher mixing ratio of Bacillus subtilis in probiotic supplementation is more effective in reducing the odor of manure.

Development of Liberal Art and Natural Science Integration Computational Thinking Education Program Based on the IoT (IoT 기반의 문·이과 통합형 CT 교육 프로그램 개발)

  • Jeong, Sang-Mok;Shin, Soo-Bum;Yim, Taek-Kyun;Mun, Seong-Yun;Jeon, In-Seong
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.4
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    • pp.255-262
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    • 2019
  • The informatics curriculum which was revised in 2015 presents the growth of creative and convergent talents as a main goal, and what is essential in the growth of creative and convergent talents is Computational Thinking(CT). In this study, in line with the goal of the growth of creative and convergent talents, the subject of IoT technology and liberal arts and natural sciences integration course was combined with the contents of informatics textbook, and the teaching-learning program was developed. In order to verify the effect of the developed teaching-learning program, the experimental research was conducted, and as a result of study, the mean of the experimental group was 10 points higher than that of the control group. Therefore, it could be known that there was an effect in the teaching-learning program suggested in this study. It is expected that the teaching-learning program suggested in this study can induce the learning motive and interest in SW education by directly implementing SW skill to the various fields of a real life through CT education based on Iot as well as a programing language, and improve convergent and scientific thinking through the experience of solving the problems which are blended with many subjects through liberal arts and natural sciences integration course, and designing them creatively.

Analysis of Passing Word Line Induced Leakage of BCAT Structure in DRAM (BCAT구조 DRAM의 패싱 워드 라인 유도 누설전류 분석)

  • Su Yeon, Kim;Dong Yeong Kim;Je Won Park;Shin Wook Kim;Chae Hyuk Lim;So won Kim;Hyeona Seo;Ju Won Kim;Hye Rin Lee;Jeong Hyeon Yun;Young-Woo Lee;Hyoung-Jin Joe;Myoung Jin Lee
    • Journal of IKEEE
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    • v.27 no.4
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    • pp.644-649
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    • 2023
  • As the cell spacing decreases during the scaling process of DRAM(Dynamic Random Access Memory), the reduction in STI(Shallow Trench Isolation) thickness leads to an increase in sub-threshold leakage due to the passing word line effect. The increase in sub-threshold leakage current caused by the voltage applied to adjacent passing word lines affects the data retention time and increases the number of refresh operations, thereby contributing to higher power consumption in DRAM. In this paper, we identify the causes of the passing word line effect through TCAD Simulation. As a result, we confirm the DRAM operational conditions under which the passing word line effect occurs, and observe that this effect alters the proportion of the total leakage current attributable to different causes. Through this, we recognize the necessity to consider not only leakage currents due to GIDL(Gate Induced Drain Leakage) but also sub-threshold leakage currents, providing guidance for improving DRAM structure.

No-Touch vs. Conventional Radiofrequency Ablation Using Twin Internally Cooled Wet Electrodes for Small Hepatocellular Carcinomas: A Randomized Prospective Comparative Study

  • Yun Seok Suh;Jae Won Choi;Jeong Hee Yoon;Dong Ho Lee;Yoon Jun Kim;Jeong Hoon Lee;Su Jong Yu;Eun Ju Cho;Jung Hwan Yoon;Jeong Min Lee
    • Korean Journal of Radiology
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    • v.22 no.12
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    • pp.1974-1984
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    • 2021
  • Objective: This study aimed to compare the efficacy between no-touch (NT) radiofrequency ablation (RFA) and conventional RFA using twin internally cooled wet (TICW) electrodes in the bipolar mode for the treatment of small hepatocellular carcinomas (HCC). Materials and Methods: In this single-center, two-arm, parallel-group, prospective randomized controlled study, we performed a 1:1 random allocation of eligible patients with HCCs to receive NT-RFA or conventional RFA between October 2016 and September 2018. The primary endpoint was the cumulative local tumor progression (LTP) rate after RFA. Secondary endpoints included technical conversion rates of NT-RFA, intrahepatic distance recurrence, extrahepatic metastasis, technical parameters, technical efficacy, and rates of complications. Cumulative LTP rates were analyzed using Kaplan-Meier analysis and the Cox proportional hazard regression model. Considering conversion cases from NT-RFA to conventional RFA, intention-to-treat and as-treated analyses were performed. Results: Enrolled patients were randomly assigned to the NT-RFA group (37 patients with 38 HCCs) or the conventional RFA group (36 patients with 38 HCCs). Among the NT-RFA group patients, conversion to conventional RFA occurred in four patients (10.8%, 4/37). According to intention-to-treat analysis, both 1- and 3-year cumulative LTP rates were 5.6%, in the NT-RFA group, and they were 11.8% and 21.3%, respectively, in the conventional RFA group (p = 0.073, log-rank). In the as-treated analysis, LTP rates at 1 year and 3 years were 0% and 0%, respectively, in the NT-RFA group sand 15.6% and 24.5%, respectively, in the conventional RFA group (p = 0.004, log-rank). In as-treated analysis using multivariable Cox regression analysis, RFA type was the only significant predictive factor for LTP (hazard ratio = 0.061 with conventional RFA as the reference, 95% confidence interval = 0.000-0.497; p = 0.004). There were no significant differences in the procedure characteristics between the two groups. No procedure-related deaths or major complications were observed. Conclusion: NT-RFA using TICW electrodes in bipolar mode demonstrated significantly lower cumulative LTP rates than conventional RFA for small HCCs, which warrants a larger study for further confirmation.

Effects of Kamut Wheat Enzyme Diet on Dextran Sulfate Sodium-induced Colitis Mice (카무트밀 효소 식이가 Dextran Sulfate Sodium으로 유발한 대장염 마우스에 미치는 효과)

  • Bum Ju Kil;Chae Yun Baek;Juni Lee;Ye Seul Hwang;Yeojin Choi;Joo Hee Son;Miae Yoo;Dong Hoon Lee;Donghun Lee
    • The Korea Journal of Herbology
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    • v.39 no.1
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    • pp.31-38
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    • 2024
  • Objectives : Ulcerative colitis is a chronic recurrent inflammatory disease of the gastrointestinal tract. However, there are some drawbacks to long-term drug therapy such as the risk of opportunistic infections. Recently, there was an increasing interest on the use of khorasan Kamut wheat because of their higher value of selenium and fiber than modern wheat. The present study was aimed to investigate the effect of Kamut brand wheat enzyme (Kamut WE) diet on colon health in dextran sulfate sodium (DSS)-induced colitis mice. Methods : Female C57BL/6J mice were divided into 6 groups. (1) normal (Water and AIN-93G diet), (2) control (1.25% DSS and AIN-93G diet), (3) Kamut WE (1.25% DSS and Kamut WE diet), (4) normal (Water and AIN-93G diet), (5) control (2.50% DSS and AIN-93G diet), (6) Kamut WE (2.50% DSS and Kamut WE diet). Dietary intake, body weight change, disease activity index (DAI), colon length and spleen weight were monitored. Results : Kamut WE group alleviated colitis symptom, including dietary intake loss, DAI (weight loss, loose stools, bleeding), colon length shortening and spleen swelling. Further, Kamut WE diets showed a significant effect against pathological damage by the increased colon length, decreased DAI and spleen weight in DSS 1.25% as well as DSS 2.50%. Conclusions : Our study provides evidence that Kamut WE diet increased colon length, decreased DAI and spleen weight in intestinal inflammation.

THE INFLUENCE OF THE VITAL BLEACHING AGENTS ON TRANSLUCENCY OF THE BOVINE ENAMEL (생활치 미백제가 우치 법랑질의 투명도에 미치는 영향)

  • Kim, Dong-Jun;Kim, Young-Ju;Kim, Hak-Geun;Park, Eun-Jong;Seo, Eun-Ju;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.30 no.3
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    • pp.178-183
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    • 2005
  • This study was done to evaluate whether vital bleaching agents could influence on the translucency of the bovine enamel. The anterior bovine teeth that were extracted one day before and without any gross discoloration were obtained and then were preserved in physiologic saline. 6 mm cylindrical tooth specimens were fabricated with diamond puncher perpendicularly on labial surface of bovine tooth. After embedded in transparent acrylic resin with labial surface being exposed, they were cut to a thickness of 1.2 mm with low speed diamond saw (Isomat, Buehler Co., Lake Bluff, IL, USA). They were smoothly ground to 1 mm thickness of enamel with sandpaper. 24 specimens were randomly divided into 3 groups and control group respectively. Opalescence (10% carbamide peroxide, Ultradent, South Jordan, USA), Rembrandt (10% carbamide peroxide, DenMat, USA) and Opalescence F (15% carbamide peroxide with fluoride, Ultradent, USA) were applied on labial sides of the bovine enamel for 7 days (bleaching agents were reapplied every 24 hours) and the opposite surface was contacted to cotton that soaked in distilled water. The control group was soaked in distilled water. Three stimulus value X, Y and Z were evaluated with colorimeter (Color ' Color Differencerneter, Model TC-6FX, Tokyo Denshoku Co., Japan) on the labial surface of all specimen three times on white and black background plate before the bleaching agents were applied and on 3rd, 5th and 7th day after applied. The degree of translucency was normally assessed by measuring the inverse property, opacity (contrast ratio). 10% Opalescence, 15% Opalescence-F, and control group showed no significant variation in the translucency of bovine enamel, However Rembrandt decreased the translucency of it (p < 0.01).

Species Composition Dynamics and Seedling Density Along Altitudinal Gradients in Coniferous Forests of Seorak Mountain (설악산 상록침엽수림의 고도별 종조성 및 치수 밀도 변화)

  • Kim, Ji-Dong;Byeon, Seong Yeob;Song, Ju Hyeon;Chae, Seung Beom;Kim, Ho Jin;Lee, Jeong Eun;Yun, I Seul;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.109 no.2
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    • pp.115-123
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    • 2020
  • The vertical distribution of vegetation can be classified according to the altitudinal gradient and the distribution of species along this gradient. The purpose of this study was to analyze the vegetation structure, species composition, dimensional density, and change according to altitude. These data illustrate the distribution of coniferous forest by altitude. By order of importance, the vegetation structure of this mixed forest consisted of Abies nephrolepis (12.2), Pinus koraiensis (10.86), and Acer komarovii (8.11). As a result of species composition according to the altitude, A. nephrolepis and Maianthemum bifolium increased in importance with increasing altitude. Tripterygium regelii emerged between 1,400 m and 1,600 m, which indicates that forest gaps were frequent at that elevation. The species diversity index was the highest from 1,400-1,500 m and coincided with the presence of forest gaps. The changes in A. nephrolepis of evergreen conifers increased significantly from 402 ± 5.4 ha.-1 to 528 ± 11.6 ha.-1 for two years, and decreased from 57 ± 1.3 ha.-1 to 56 ± 1.6 ha.-1 for P. koraiensis. The density of A. nephrolepis and P. koraiensis seedlings significantly increased at 1,500-1,600 m. The results of this study can be used as a basis to identify the mast seeding year with the increase or decrease of seedlings. In addition to documenting the evergreen conifer population of the Seorak Mountain, these results can be built upon for future monitoring of seedlings mortality.

Chemical Characteristics, Antimicrobial Activity and Dyeability of Gyeongsanbansi (Persimmon kaki) Unripe Juice Extraction and Fermented Liquor (경산반시의 미숙감 착즙액과 발효액의 화학적 특성, 항균성 및 염색성)

  • Heo, Buk-Gu;Park, Yun-Jum;Kim, Tae-Choon;Kim, Hyun-Ju;Park, Su-Min;Jang, Hong-Gi;Kim, Kyung-Su;Lee, Kyung-Dong;Yun, Jae-Gill
    • Korean Journal of Plant Resources
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    • v.22 no.5
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    • pp.438-445
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    • 2009
  • This study was conducted to examine the chemical characteristics, antibacterial activity and dyeability of several juices made from Persimmon cv. 'Gyeongsanbanshi'. Young fruits of persimmon were harvested at 1st August, 2006. Fruit juice was extracted soon after harvesting, some of them were stored at $4{\sim}6^{\circ}C$ for 6 months or fermented at room temperature for 6 months. $L^*$ values of vinegar was 43.5 higher than those of fresh juice and fermented liquor. Inorganic matter contents in fresh juice, fermented liquor and vinegar were higher in the order of K ($1696{\sim}2880\;mg$/100 g), Ca ($166.7{\sim}417.9\;mg$/100 g), Mg ($203.9{\sim}214.4\;mg$/100 g), P ($37.9{\sim}109.8\;mg$/100 g), Na ($13.2{\sim}23.3\;mg$/100 g) and Fe ($8.4{\sim}14.2\;mg$/100 g). Cotton fabrics dyed with the fermented liquor and vinegar had the largest inhibitory zone against the gram-positive microorganisms with range of $16.0{\sim}35.0\;mm$. Cotton fabrics dyed with the fresh juice showed $9.0{\sim}9.5\;mm$ inhibitory zone against the gram-negative microorganisms, $15.0{\sim}21.0\;mm$ with the juice stored at $4{\sim}6^{\circ}C$ for 6 months, $22.0{\sim}23.0\;mm$ with the fermented liquor and $9.0{\sim}35.0\;mm$ with vinegar. The hue of cotton fabrics dyed with the fermented liquor had a YR levels, and antibacterial activity of them were 78.5%.

Effect of organic fertilizer application on soil carbon accumulation (유기질비료의 사용이 작물의 생육, 토양화학성 및 토양탄소 축적량에 미치는 영향)

  • Yu Na Lee;Dong Won Lee;Jin Ju Yun;Jae Hong Shim;Sang Ho Jeon;Yun Hae Lee;Soon Ik Kwon;Seong Heon Kim
    • Journal of the Korea Organic Resources Recycling Association
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    • v.32 no.1
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    • pp.5-11
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    • 2024
  • Objective of this study was to evaluate the effect of organic fertilizer application on yield, soil chemical properties and soil organic carbon (SOC) in Korean cabbage cultural field. The experimental treatments consisted of none fertilizer (NF), NPK (inorganic fertilizer, N-P2O5-K2O : 320-78-198 kg ha-1), Organic fertilizer (OF 50, 100, 150% on application rate of standard 110 kg ha-1 as N, topdressing: 210 kg ha-1 as inorganic fertilizer). In experimental results, the growth characteristics and yields were not significantly different among the treatments. There was no significant difference in soil pH, available phosphate, ammonium nitrogen and exchangeable potassium, while organic matter, electrical conductivity and nitrate nitrogen were increased when organic fertilizer application. Also, SOC was increased with the application of organic fertilizers. These results showed that pre-application of organic fertilizer might be effective in a carbon storage in the field soil cultivating Korean cabbage.