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Mycelial and cultural characteristics of Pleurotus ostreatus 'Baekseon', a novel white cultivar for bottle culture (병재배용백색느타리신품종 『백선』의 균사배양및생육특성)

  • Choi, Jong In;Lee, Yun Hae;Gwon, Hee Min;Jeon, Dae Hoon;Lee, Yong Seon;Lee, Young Sun
    • Journal of Mushroom
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    • v.17 no.3
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    • pp.113-118
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    • 2019
  • Oyster mushrooms are an economically important crop, accounting for 35% of the total mushroom production in Korea. In this study, we developed a new cultivar of Pleurotus ostreatus, known as 'Baekseon,' which is characterized by a white pileus with a white stipe. It was bred by mating monokaryons isolated from white mutant oyster mushrooms that were naturally generated from 'Gonji-7ho' and 'Wonhyeong-1ho' at the Mushroom Research Institute, GARES, Korea in 2018. The optimum temperature for mycelial growth on potato dextrose agar medium was approximately $28-31^{\circ}C$, and the optimum temperatures for primordia formation and growth of fruit bodies on sawdust media were $22^{\circ}C$ and $20^{\circ}C$, respectively. The time required for the bottle-cultured mushrooms to complete spawn running, primordia formation, and growth of fruit bodies was 30 days, 4 days, and 4 days, respectively. The fruit bodies were bundle-shaped, the pilei were round type and white, and the stipes were white. The stipes were slender and longer than those of the control ('Miso'). In the productivity test, the yield per bottle was 185 g/1100 mL, which was 45% greater than that of the control ('Miso'). In the farm test, the yield per bottle for Farm A (Pyeongtaek) and Farm B (Yeoju) was 184 g/1100 mL and 178 g/850 mL, respectively. With regard to the physical properties of fruit bodies, the springiness, cohesiveness, gumminess, and brittleness of stipe tissue were 80%, 57%, 720 g, and 57 kg, respectively. These values were lower than those of the control ('Miso'). To test the shelf life, the fruit bodies were wrapped with antifogging film and stored at $4^{\circ}C$ for 28 days and then at room temperature for 4 days; such conditions were sufficient for maintaining edibility.

Floristic Composition, Structure, and Dynamics of Koelreuteria paniculata Communities in Daegu City (대구시 자생 모감주나무군락의 종조성, 구조 및 동태)

  • Kim, Jun-Soo;Cho, Joon-Hee;Kim, Hak-Yun;Cho, Hyun-Je
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.168-176
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    • 2019
  • To understand the floristic composition, vegetation structure, and population dynamics of Koelreuteria paniculata communities, which are native to Daegu, South Korea, a field survey was conducted in July 2018 using phytosociological and complete enumeration methods. Based on information on vegetation and trees of >5 cm diameter at breast height collected during the field survey, we classified the community types by species composition and analyzed their vegetation strata, relative importance value (MIV), life forms, species diversity, and population structure. The community was divided into the following three types: Ulmus parvifolia-Vitex negundo var. incisa subcommunity, Securinega suffruticosa subcommunity, and Clematis apiifolia community. The vegetation unit system was organized into one community group, two communities, and two subcommunities. Vegetation coverage of the tree layer was >85%, while the herbaceous layer was <10%. MIV of K. paniculata appeared to be extremely high within all vegetation strata, with 94.3 of the tree layer, 81.6 of the subtree layer, 75.5 of the shrub layer, and 60.0 of the herbaceous layer. The species diversity (H') was significantly different among the community types, and the C. apiifolia community (2.062) was approximately four times higher than the S. suffruticosa subcommunity (0.547). The overall representative life form types were "$MM-R_5-D_4{\cdot}D_2-e$,", but there were some differences in the disseminule form among the community types. The population structure of K. paniculata showed the reverse J-shaped distribution with a high density of young individuals and low density of larger individuals among all three community types, and because no plant species within the lower vegetation could replace K. paniculata, it was considered to be a sustainable population.

Changes in Composition and Structure of Forest Vegetation of Apsan Park, Daegu Metropolitan City (대구광역시 앞산공원 산림식생의 조성 및 구조 변화)

  • Oh, Jeong Hak;Kim, Jun-Soo;Kim, Hak Yun;Cho, Hyun Je
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.177-188
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    • 2019
  • The aim of our study was to identify the changing trends in the composition, structure, and spatial distribution of forest vegetation in Apsan Park, a representative urban forest in Daegu, South Korea. A vegetation survey was conducted in 1997 and 2016 using phytosociological methods, and a detailed vegetation map was created using the physiognomic dominant species. There were 22 vegetation types in both 1997 and 2016, but two of those types increased and two decreased. The total coverage per unit area ($100m^2$) of the component vegetation species increased from 163% in 1997 to 182% in 2016, and natural vegetation tended to be more than twice that of artificial vegetation. The average number of species decreased by seven from 25 in 1997 to 18 in 2016. Species diversity (H') increased only slightly from 1,654 in 1997 to 1,680 in 2016, while species dominance (D) decreased by 9% from 0.304 in 1997 to 0.276 in 2016. The similarity in the composition of the forest vegetation was about 78%, which was nearly the same. The life form spectrums of vascular plants changed from '$G-R_5-D_4-e$' in 1997 to '$MM-R_5-D_4-e$' in 2016 and the central dormancy type changed from geophytes (G) to megaphanerophytes (MM). The spatial distribution of the forest vegetation was reduced by approximately four times that of artificial vegetation. The number of forest landscape elements (patches) increased from 269 in 1997 to 294 in 2016, while the average area decreased by 12% from 5.8 ha in 1997 to 5.1 ha in 2016.

Forest Vegetation Structure in Maruguem (the Ridge Line) Area of Gitdaebaegibong to Jukryeong, Baekdudaegan (백두대간(깃대배기봉-죽령 구간) 마루금 주변의 산림식생구조)

  • Song, Ju Hyeon;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.147-167
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    • 2019
  • This study was conducted to analyze forest vegetation structure in the Marugeum (Ridge) area of Gitdaebaegibong to Jukryeong, Baekdudaegan. Data were collected in 298 quadrates through a Braun-Blanquet vegetation survey from April, 2018 to October, 2018. Forest vegetation was classified into 13 vegetation units. A Quercus mongolica community was divided into Morus bombycis, Filipendula glaberrima, Fraxinus sieboldiana, Prunus maackii unit and Q. mongolica typical unit. The M. bombycis unit was further classified into a Deutzia glabrata group and M. bombycis typical group. The F. glaberrima unit was subdivided into a Veratrum oxysepalum group, Arundinella hirta group, and F. glaberrima typical group. The F. sieboldiana unit was divided into a Pinus densiflora group, Larix kaempferi group, and F. sieboliana typical group. The relationship between vegetation units and environmental factors was studied through coincidence analysis and CCA. The F. glaberrima unit (VU 6~8) was distributed by elevation above 1,200 m and other vegetation units were distributed below 1,200 m. Results of the CCA analysis showed that the F. glaberrima unit distribution is positively correlated with elevation. As a result of species diversity, the F. glaberrima unit was higher than other vegetation units. A similarity index analysis revealed that the F. sieboldiana unit (VU 9~11) was relatively homogeneous, and the M. bombycis unit (VU 1~5) and A. girta group (VU 7) were relatively heterogeneous. A detrended correspondence analysis determined that the distance between the statistical axes of the M. bombycis and F. glaberrima units was the greatest, which is consistent with the analysis of the similarity index. As a result of interspecific correlation of major woody plants, hydrophilic species were positively correlated, and a negative correlation was found between Q. mongolica and intolerant species such as P. densiflora and L. kaempferi.

Establishment of Geospatial Schemes Based on Topo-Climatology for Farm-Specific Agrometeorological Information (농장맞춤형 농업기상정보 생산을 위한 소기후 모형 구축)

  • Kim, Dae-Jun;Kim, Soo-Ock;Kim, Jin-Hee;Yun, Eun-Jeong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.146-157
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    • 2019
  • One of the most distinctive features of the South Korean rural environment is that the variation of weather or climate is large even within a small area due to complex terrains. The Geospatial Schemes based on Topo-Climatology (GSTP) was developed to simulate such variations effectively. In the present study, we reviewed the progress of the geospatial schemes for production of farm-scale agricultural weather data. Efforts have been made to improve the GSTP since 2000s. The schemes were used to provide climate information based on the current normal year and future climate scenarios at a landscape scale. The digital climate maps for the normal year include the maps of the monthly minimum temperature, maximum temperature, precipitation, and solar radiation in the past 30 years at 30 m or 270 m spatial resolution. Based on these digital climate maps, future climate change scenario maps were also produced at the high spatial resolution. These maps have been used for climate change impact assessment at the field scale by reprocessing them and transforming them into various forms. In the 2010s, the GSTP model was used to produce information for farm-specific weather conditions and weather forecast data on a landscape scale. The microclimate models of which the GSTP model consists have been improved to provide detailed weather condition data based on daily weather observation data in recent development. Using such daily data, the Early warning service for agrometeorological hazard has been developed to provide weather forecasts in real-time by processing a digital forecast and mid-term weather forecast data (KMA) at 30 m spatial resolution. Currently, daily minimum temperature, maximum temperature, precipitation, solar radiation quantity, and the duration of sunshine are forecasted as detailed weather conditions and forecast information. Moreover, based on farm-specific past-current-future weather information, growth information for various crops and agrometeorological disaster forecasts have been produced.

A Study on the Locations of Jagiso and Dogiso in Najumok Recorded in the 'Sejongsylrok Jiliji' (『세종실록(世宗實錄)』 「지리지(地理志)」 나주목(羅州牧)의 자기소(磁器所)·도기소(陶器所) 위치 연구)

  • Sung, Yun-gil
    • Korean Journal of Heritage: History & Science
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    • v.52 no.3
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    • pp.4-37
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    • 2019
  • Buncheong Ware, which was produced for approximately 200 years from the end of the Goryeo Dynasty to the beginning of the Joseon Dynasty, was used for royal house and state affairs during the early Joseon Dynasty. Records of Buncheong Ware can be found with Jagiso and Dogiso, considered the place of production, in the 'Sejongsylrok jiliji'. This is notable, unlike celadon and white porcelain. However, compared to Gyeongsangnam-do and Gyeongsangbuk-do, the study of Jagiso and Dogiso in Jeollanam-do and Jeollabuk-do has been unsatisfactory. In this paper, we examined the locations of Jagiso and Dogiso in the Jeollanam-do prefecture. This study is focused on Najumok, located in the western part of Jeollanam-do. The following research results were obtained: First, the locations of Jagiso and Dogiso in nine areas were examined. These nine areas comprised Najumok and its immediate provinces of Haejingun, Yeongamgun, Yeonggwanggun, Hampyeonghyeon, Muanhyeon, Gochanghyeon, Heungdeokhyeon and Jangseonghyeon. Previous research results exist for some of the regions; however, only Buncheong Ware was used to estimate Jagiso and Dogiso. In this paper, we tried to discover a more accurate position of Jagiso and Dogiso through a comparison of the place names recorded in the 'Sejongsylrok jiliji' and the place names recorded in the 'Hoguchoungsu'. Also, pottery-related area names and Buncheong Ware obtained from the field survey were reviewed. Table 2 shows the specific positions of Jagiso and Dogiso in the areas examined. Second, it would appear that pottery was produced not only in Jagiso but also in Dogiso. This is because much of Buncheong Ware was unearthed in Najumok, Yeongamgun, and Muanhyeon Dogiso. However, it was only excavated from the Buncheong Kiln Site in Yucheon Village, Sangweol-ri, Yeongam-gun; there could be limitations regarding to the rest of the areas because only a field survey was conducted, not an excavation. A recent excavation at Donggok Village in Cheongdo-ri, Gimje also confirmed a Buncheong Kiln Site. There were two Buncheong Ware ('公', '上') artifacts excavated from the site, believed to be Gongnapjagi. In addition, Cheongdo-ri, where the Buncheong Kiln Site is located, was found to be an area in Wurimgokmyeon, a former Wurimgok at a Dogiso in Jeonjubu. Consequently, it would seem that Dogiso also produced porcelain (Buncheong Ware). If more data can be obtained from the excavations at sites considered to be Dogiso, the results of Dogiso-related research will be strengthened. Third, in previous surveys, field surveys and excavation surveys were based on estimates using artifacts, but the name of the location of the Jagiso and Dogiso may be confirmed more accurately by tracking the changes in the names of the administrative areas. Therefore, although it is important to conduct research using the results of surveys or excavations, changes in the place names or places related to porcelain must not be overlooked.

Pseudomonas tolaasii bacteriophage-specific polyclonal antibody formation and its cross reactivity to various phages (Pseudomonas tolaasii 박테리오파지에 특이적인 다클론항체 형성 및 이를 이용한 파지 교차 반응성)

  • Yun, Yeong-Bae;Park, Soo-Jin;Kim, Young-Kee
    • Journal of Applied Biological Chemistry
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    • v.62 no.3
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    • pp.287-292
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    • 2019
  • Pseudomonas tolaasii causes brown blotch disease on the oyster mushroom (Pleurotus ostreatus). Various pathogenic strains of P. tolaasii were isolated and divided into three subtypes, $P1{\alpha}$, $P1{\beta}$, and $P1{\gamma}$. For phage therapy, bacteriophages against to these subtype strains were applied to mushroom cultivation and very successful to prevent from the disease. In this study, bacteriophages were isolated against the representative strains of subtype pathogens and their polyclonal antibodies were synthesized to investigate structural relationship among capsid proteins of phages. Phage preparations over $10^{10}pfu/mL$ were injected to rabbit thigh muscle and polyclonal antibodies were obtained after three times of boost injection. Titers of the antibodies obtained were over $2{\times}10^7Ab/mL$ for the phage ${\phi}6264$, $1{\times}10^6Ab/mL$ for the phage ${\phi}HK2$, and $1{\times}10^7Ab/mL$ for the phage ${\phi}HK19$ and phage ${\phi}HK23$. High specific activities were observed between antibodies and the corresponding bacteriophages. Some cross-reactivities between the antibodies and non-corresponding bacteriophages were also measured. Antibody $Ab{\phi}6264$ inactivated all phages of $P1{\alpha}$ subtype and only phage ${\phi}HK16$ among $P1{\beta}$ subtype phages. Antibody $Ab{\phi}HK23$ of $P1{\gamma}$ subtype neutralized all phages of $P1{\beta}$ subtype as well as the phage ${\phi}HK23$, showing the widest phage-inactivation range. When the structural-similarity studies of phages were investigated by using phage antibodies, closeness obtained by phylogenetic analysis of 16S rRNA genes of pathogenic strains were quite different from that of polyclonal antibody-specific structural similarity of phage capsid proteins. In conclusion, there is weak correlation between the host strain specificity of bacteriophage and its capsid structural similarity measured by phage antibodies.

A Study on the Development of Assessment Index for Catastrophic Incident Warning Sign at Refinery and Pertrochemical Plants (정유 및 석유화학플랜트 중대사고 전조신호 평가지표 개발에 관한 연구)

  • Yun, Yong Jin;Park, Dal Jae
    • Korean Chemical Engineering Research
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    • v.57 no.5
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    • pp.637-651
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    • 2019
  • In the event of a major accident such as an explosion in a refinery or a petrochemical plant, it has caused a serious loss of life and property and has had a great impact on the insurance market. In the case of catastrophic incidents occurring in process industries such as refinery and petrochemical plants, only the proximate causes of loss have been drawn and studied from inspectors or claims adjustors responsible for claims of property insurers, incident cause investigators, and national forensic service workers. However, it has not been done well for conducting root cause analysis (RCA) and identifying the factors that contributed to the failure and establishing preventive measures before leading to chemical plant's catastrophic incidents. In this study, the criteria of warning signs on CCPS catastrophic incident waning sign self-assessment tool which was derived through the RCA method and the contribution factor analysis method using the swiss cheese model principle has been reviewed first. Secondly, in order to determine the major incident warning signs in an actual chemical plant, 614 recommendations which have been issued during last the 17 years by loss control engineers of global reinsurers were analyzed. Finally, in order to facilitate the assessment index for catastrophic incident warning signs, the criteria for the catastrophic incident warning sign index at chemical plants were grouped by type and classified into upper category and lower category. Then, a catastrophic incident warning sign index for a chemical plant was developed using the weighted values of each category derived by applying the analytic hierarchy process (pairwise comparison method) through a questionnaire answered by relevant experts of the chemical plant. It is expected that the final 'assessment index for catastrophic incident warning signs' can be utilized by the refinery and petrochemical plant's internal as well as external auditors to assess vulnerability levels related to incident warning signs, and identify the elements of incident warning signs that need to be tracked and managed to prevent the occurrence of serious incidents in the future.

Impact of Entrepreneurial Business Start-up Motivation, Entrepreneurial Spirit, and Entrepreneurial Competence Characteristics on Start-up Companies' Sustainability: Focusing on the Mediating Effect of the Start-up Companies' Business Performance (창업자의 창업동기, 창업가정신 그리고 창업가 역량특성이 창업기업 지속가능성에 미치는 영향: 창업기업 경영성과를 매개로 하여)

  • Hyuk, Kang Han;Park, Woojin;Yun, Bae byung
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.3
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    • pp.59-71
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    • 2019
  • The domestic unemployment rate is now seriously increasing due to the impact of the international trend of incurring high costs using only a small number of manpower in a situation where the business has been being reduced in size. The nation is taking start-up promotion policies as a way to overcome the problem, but with the advent of excessive entrepreneurs and their concentration on specific areas, their survival is in an unclear situation. Thus, this study was intended to provide a useful direction for successful start-ups by identifying the impact of entrepreneurial motivation and entrepreneurship, internal characteristics of entrepreneurs, and of competency characteristics, external characteristics of them, on sustainability through business performance. Thus, the purpose of this study was to provide a useful direction for successful start-ups by identifying the impact of entrepreneurial motivation and entrepreneurship, internal characteristics of entrepreneurs, competency, and external characteristics, on sustainability through business performance. The result of verifying the hypothesis has showed that entrepreneurial motivation, entrepreneurship, and competency characteristics had a positive effect on business performance, and on the other hand, the business performance had a positive effect on sustainability. In addition, in terms of the impact of entrepreneurial motivation, entrepreneurship, and competency characteristics on sustainability, all of them had mediating effects on business performance. It is obvious that studies on the factors affecting business performance and corporate sustainability up to now have been carried out by only collecting the independent variables of this study individually or two of them. so it is judged that if the study, which has verified the practical direction in general through the verification of entrepreneurs's internal and external characteristics from various angles, is performed more comprehensively along with the addition of background characteristics of entrepreneurs, such as characteristics of start-up preparation, etc., in the future, more in-depth results will be able to be obtained.

A Study on Usability of Open Source Software for Developing Records System : A Case of ICA AtoM (공개 소프트웨어를 이용한 기록시스템 구축가능성 연구 ICA AtoM을 중심으로)

  • Lee, Bo-Ram;Hwang, Jin-Hyun;Park, Min-Yung;Kim, Hyung-Hee;Choi, Dong-Woon;Choi, Yun-Jin;Yim, Jin-Hee
    • The Korean Journal of Archival Studies
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    • no.39
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    • pp.193-228
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    • 2014
  • In recent years, as well as management of public records, interest in the private archive of large and small is growing. Dedicated archive has various types. In addition, lack of personnel and budget, personnel records management professional because the absence, that help you maintain these records in a systematic manner is not easy. Request to the system have continued to rise, but the budget and professionals in order to solve this problem are missing. As breakthrough of the burden to the system with archive dedicated, it introduces the trends and meaning of public recording system, and was examined in detail AtoM function. AtoM is public land can be made by a method that requires a Web service, the database server. Without restrictions, including the advantage of being available free of charge, by the application or operating system specific, installation and operation is convenient. In addition, compatibility, and is highly scalable, AtoM use and convenient archive of private experiencing a shortage of personnel and budget. Because in terms of data management, and excellent interoperability and search share, and use, it is possible in the future, it favors also documentary use through a network of inter-agency archives and private. In addition, Enhancements exhibition services through cooperation with Omeka, long-term storage through Archivematica, many discussion is needed. Public centered around the private area of the recording management spilling expanded, open-source software allows to balance the recording system will be able to play an important role. In addition, the efforts of academia and in the field, close collaboration between the open source recording system through a user study should be continued. Furthermore, co-operation and sharing of private archives expect come true.