• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1308-1315
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• 2007

Lipase B from Candida antarctica (CalB) displayed on the cell surface of H. polymorpha has been functionally improved for catalytic activity by molecular evolution. CalB was displayed on the cell surface by fusing to a cell-wall anchor motif (CwpF). A library of CalB mutants was constructed by in vivo recombination in H. polymorpha. Several mutants with increased whole-cell CalB activity were acquired from screening seven thousand transformants. The two independent mutants CalB 10 and CalB 14 showed an approximately 5 times greater whole-cell activity than the wild-type. When these mutants were made as a soluble form, CalB 10 showed 6 times greater activity and CalB 14 showed an 11 times greater activity compared with the wild-type. Sequence analyses of mutant CALB genes revealed amino acid substitutions of $Leu^{278}Pro$ in CalB10 and $Leu^{278}Pro/Leu^{219}Gln$ in CalB14. The substituted $Pro^{278}$ in both mutants was located near the proline site of the ${\alpha}$10 helix. This mutation was assumed to induce a conformational change in the ${\alpha}$10 helix and increased the $k_{cat}$ value of mutant CalB approximately 6 times. Site-directed mutagenized CalB, LQ ($Leu^{219}Gln$) was secreted into the culture supernatant at an amount of approximately 3 times more without an increase in the CalB transcript level, compared with the wild-type.

• 임상간호연구
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• 제22권3호
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• pp.285-293
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• 2016

Purpose: The purpose of this study was aimed to develop evidence-based nursing practice guideline for preventing intermittent urinary catheterization (IUC) related complications using guideline remake process. Methods: Guideline remake process was conducted according to guideline adaptation manual developed by Gu et al (2012) which consisted of three main phases and 9 modules including a total of 24 steps. Results: Newly developed IUC guideline consists of introduction, overview of intermittent catheterization, summary of recommendations, recommendations, references, and appendices. There were 50 recommendations in 5 sections including assessment, equipments, catheterization, complications management, and education/consult. Three recommendations (6%) were graded A, and five (10%) and 41 recommendations(82%) were B and C, respectively. Conclusion: The IUC remade-guideline was developed based on evidence-based nursing and therefore, this guideline is recommended to be disseminated and utilized by nurses nationwide to improve the quality of care for IUC and to decrease the IUC related complications.

• 한국환경과학회지
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• 제21권6호
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• pp.739-747
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• 2012

Due to the difficulties for measuring flood discharge in the dangerous field conditions, conventional instruments with relatively low accuracy such as float still have been widely utilized for the field survey. It is also limited to use simple stage-discharge relationship for assessment of the flood discharge, since the stage-discharge relationship during the flood becomes complicated loop shape. In recent years, various non-intrusive velocity measurement techniques such as electromagnetic wave or surface images have been developed, which is quite adequate for the flood discharge measurements. However, these new non-intrusive techniques have little tested in the flood condition, though they promised efficiency and accuracy. Throughout the field observations, we evaluated the validity of these techniques by comparing discharge and velocity measurements acquired concurrently during the flood in a mountain stream. As a result, the flood discharge measurements between electromagnetic wave and surface image processing techniques showed high positive relationship, but velocities did not matched very well particularly for the high current speed more 3 m/s. Therefore, it should be noted here that special cares are required when the velocity measurements by those two different techniques are used, for instance, for the validation of the numerical models. In addition, authors assured that, for the more accurate flood discharge measurements, velocity observation as well as stage height is strongly necessary owing that the unsteady flow occurs during the flood.

• 환경영향평가
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• 제7권2호
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• pp.5-14
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• 1998

This study was performed to estimate interrelation between characteristics of sediment and nutrient releare from sediment in Dae-cheong lake. For the investigations, sediments were sampled in June and October 1997 at fish farms, embayment, and the main stream of Dae-cheong lake. Items for investigation are as follows; water content, weight loss on ignition(IG), porosity of sediment, Total Kjeldahl Nitrogen(TKN), content of element(H, N, C), nutrient release rate. Water content and porosity were measured to conjecture the physical trait and grain size. And weight loss on ignition was measured to determine the contents of organic substance. For the determination of nutrient release rate, $PO_4-P$ and $NH_4-N$ concentration of interstitial water and overlying water were measured. Release rate of nutrients which has direct influenced upon the water quality were 0.05-8.63mg-$P/m^2{\cdot}day$ and 4.99-36.56mg-$N/m^2{\cdot}day$. And it was found that release rate was measured higher in the 1st sampling than in the 2nd sampling. And for determination of the humus level of sediment, carbon and nitrogen content were measured by using elemental analyzer. Generally, C/N ratio is used to determine humus level of lake sediment. As a result of elemental analysis, C/N ratio was determined in the range of 7.64~11.55, so humus level of Dae-cheong lake sediment was estimated from mesohumic state to oligohumic state.

• Molecules and Cells
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• 제22권2호
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• pp.182-188
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• 2006

Dlx5 and Osx are master regulatory proteins essential for initiating the cascade leading to osteoblast differentiation in mammals, but the mechanism of osteoblast-specific expression is not fully understood. DNA methylation at CpG sequences is involved in tissue and cell type-specific gene expression. We investigated the methylation status of Dlx5 and Osx in osteogenic and nonosteogenic cell lines by methylationspecific PCR (MSP). The CpG dinucleotides of the Dlx5 and Osx promoter regions were unmethylated in osteogenic cell lines transcribing these genes but methylated in nonosteogenic cell lines. Treatment of C2C12 cells with 5-AzadC induced dose- and timedependent expression of Dlx5 and Osx mRNA by demethylating the corresponding promoters. Furthermore the mRNAs for the osteoblast markers ALP and OC, which were undetectable in untreated cells, gradually increased after 5-AzadC treatment. In addition, BMP-2 stimulation induced Dlx5 expression by hypomethylating its promoter. These findings suggest that DNA methylation plays an important role in cell type-specific expression of Dlx5 and Osx.

• 한국지반공학회논문집
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• 제37권12호
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• pp.89-105
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• 2021

본 논문에서는 흙막이 시설물 내진설계의 필요성을 살펴보기 위한 기초 연구로서, 유한차분해석 프로그램인 FLAC을 이용하여 가상의 대심도 흙막이 구조물을 대상으로 내진해석을 수행하고, 지진하중이 흙막이 시설물의 동적 거동에 미치는 영향을 평가하였다. 그 결과 지진하중 작용 시 벽체에 발생하는 모멘트와 지보재의 최대 축력이 지진하중 작용 전 최종 굴착단계와 비교하여 각각 98%, 87%까지 증가하는 것으로 나타나, 동적 토압이 구조물에 미치는 영향이 매우 큰 것으로 분석되었다. 또한 동해석 결과로 얻은 부재력을 이용하여 현행 기준에 따라 설계된 흙막이의 안정성을 재평가하고, 지진하중이 구조물의 설계에 미치는 영향을 분석하였다.

• Journal of the Korean Wood Science and Technology
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• 제49권4호
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• pp.371-383
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• 2021

Plant essential oils are used in products such as fragrances and cosmetics due to their individual aromatic characteristics. Currently, essential oils are not only used in cosmetics but also in pharmaceutical products with anti-bacterial, anti-viral, anti-fungal, anti-parasitic, insecticidal, anti-cancer, neuroprotective, psychophysiological, or anti-aging effects. Despite their pharmaceutical properties, some studies reported cytotoxic effects in high doses. Therefore, for pharmaceutical purposes, the margin of safety of essential oils needs to be examined. Herein, we evaluated the IC₅₀ of 10 essential oil from Korean native plants: Juniperus chinensis L. var. sargentii Henry, Citrus natsudaidai Hayata, Citrus reticulata Blanco, Citrus unshiu (Yu. Tanaka ex Swingle) Marcow, Artemisia capillaris Thunb, Aster glehnii F. Schmidt, Juniperus chinensis L, Zanthoxylum schinifolium Siebold & Zucc, Zanthoxylum piperitum (L.) D, and Cinnamomum loureirii. In addition, gene regulation of the cell-cycle gene and apoptosis marker CASP3 was examined at the IC₅₀ level. The purpose of this study was to describe the toxic concentrations of essential oils extracted from Korean native plants, thereby providing toxic concentration guidelines for inclusion in a toxicity database and in the application of plant essential oils in various fields.

• 한국수자원학회논문집
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• 제45권9호
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• pp.853-860
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• 2012

홍수 발생 시 모래하천의 수위-유량 관계가 고리형을 이룬다는 것은 많은 현장 계측을 통해 보고되어 왔다. 또한, 홍수파 해석이나 사련에서 사구로 변동되는 하상해석을 통해 고리형 수위-유량 관계의 원인이 규명되어서 고리의 형태나 진행방향 등에 대해서는 이론적으로도 상당부분 입증되어 있다. 그러나 경사가 급한 자갈 및 암반으로 구성된 산지하천의 경우, 현장유량계측의 어려움과 관측 정확도의 문제로 고리형 수위-유량 관계에 대한 연구가 매우 드물었다. 본 논문은 자갈 및 암반지형의 산지하천인 제주도 한천에서 2011년 태풍 무이파 때 계측한 홍수 유량을 바탕으로 수위-유량 관계를 구축하고, 자갈하천에서 형성되는 고리형 수위-유량곡선을 분석하고자 한다. 유량계측 방식으로는 홍수기에 적합한 비접촉식인 전자파 표면유속계를 사용하여, 홍수의 상승기와 하강기의 유량을 시간 단위로 계측하는 데 성공했다. 계측결과 수위-유량관계에서 뚜렷한 고리 형상을 확인할 수 있었다. 그리고 고리의 이력이 모래하천과 정반대로 동일수위에서 상승기의 유량이 하강기의 유량보다 작은, 고리 경로의 역전 현상을 발견하였다. 이러한 역전현상은 자갈하천의 조도의 변화가 모래하천의 경우와 반대로 발생하는 데 기인하는 것으로 추정한다.

• 대한임상검사과학회지
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• 제50권3호
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• pp.359-369
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• 2018

의료 관련 감염을 예방하기 위한 필수적 방법은 일관성이 있는 감염감시 시스템을 구축하고 효율적인 감시 통제를 수행하기 위해 신뢰할 수 있는 상황에 대해 진단을 향상시켜나가는 것이다. 또한 의료 종사자의 손과 의복 및 장비는 환자 관리 및 환경과 접촉하여 병원체가 오염되는 요인이다. 병원체를 가진 시설 표면(예: 침대 레일, 침대 옆 탁자), 음용수, 냉각탑용수, 내시경기구, 급식위생, 공기매개, 멸균검사, 내독소검사, 및 의료 장비를 포함한 의료 환경의 오염은 일반적으로 발생한다. 또한 이러한 감염원을 활동 감시를 통해 MLST, PFGE의 기법으로 역학분석을 수행한다. 따라서 HAI 예방을 위한 환경 감시배양 검사는 환자의 안전과 감염원의 차단을 향상시킬 뿐만 아니라 국가의 감염관리 시스템을 통제하여 최적의 효율적인 감염관리 예방을 가능케 하고 국가의 감염관리 시스템의 안전을 향상시킨다. 결론적으로 감시배양 검사의 표준화를 통해 실효성 있는 감염관리체계에 이바지하고 감염관리 전문인력으로서의 전문성을 확보하고자 한다. 이를 통해 표준화 마련의 일차적인 목표는 의료관련 감염을 줄이고 국가적 의료관리 체계를 향상시키는데 있다.

• 한국환경과학회지
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• 제20권6호
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• pp.783-788
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• 2011

Different from the main land of South Korea, Jeju Island has been in difficulties for measuring discharge. Due to high infiltration rate, most of streams in Jeju Island are usually in the dried state except six streams with the steady base flow, and the unique geological characteristics such as steep slope and short traveling distance of runoff have forced rainfall runoff usually to occur during very short period of time like one or two days. While discharge observations in Jeju Island have been conducted only for 16 sites with fixed electromagnetic surface velocimetry, effective analysis and validation of observed discharge data and operation of the monitoring sites still have been limited due to very few professions to maintain such jobs. This research is sponsored by Ministry of Land, Transport and Maritime Affairs to build water cycle monitoring and management system of Jeju Island. Specifically, the research focuses on optimizing discharge measurement techniques adjusted for Jeju Island, expanding the monitoring sites, and validating the existing discharge data. First of all, we attempted to conduct discharge measurements in streams with steady base flow, by utilizing various recent discharge monitoring techniques, such as ADCP, LSPIV, Magnetic Velocimetry, and Electromagnetic Wave Surface Velocimetry. ADCP has been known to be the most accurate in terms of discharge measurement compared with other techniques, thus that the discharge measurement taken by ADCP could be used as a benchmark data for validation of others. However, there are still concerns of using ADCP in flood seasons; thereby LSPIV would be able to be applied for replacing ADCP in such flooded situation in the stream. In addition, sort of practical approaches such as Magnetic Velocimetry, and Electromagnetic Wave Surface Velocimetry would also be validated, which usually measure velocity in the designated parts of stream and assume the measured velocity to be representative for whole cross-section or profile at any specified location. The result of the comparison and analysis will be used for correcting existing discharge measurement by Electromagnetic Wave Surface Velocimetry and finding the most optimized discharge techniques in the future.