• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.110-111
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• 2008

• The Korean Journal of Mycology
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• v.50 no.3
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• pp.249-254
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• 2022

In June 2021, foot rot symptoms were observed in wasabi (Eutrema japonicum) plants growing in vinyl greenhouses of the Alpine Agricultural Experiment Station, Wild Vegetable Research Institute, in Taebaek, Gangwon Province, Korea. Diseased plants displayed black soft rot of crowns and petioles at the soil line and wilted. The incidence of diseased plants was 2-10% in four out of five vinyl greenhouses investigated. Eight fungal isolates were obtained from diseased plants. All the isolates were identified as Phytophthora pseudocrytogea based on their morphological characteristics and phylogenetic analysis. Three isolates of P. pseudocrytogea were used for pathogenicity test on wasabi plants via artificial inoculation. The pathogenicity of the isolates was confirmed in the inoculated wasabi plants. The symptoms shown by the inoculated plants were similar to those observed in plants from the investigated vinyl greenhouses. This is the first report of P. pseudocryptogea causing Phytophthora foot rot in wasabi.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.540-543
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• 2007

We have investigated the vegetation in the peninsula located in Ongjeong-Li Seomyeon in Youngweol-gun. Since this is a limestone area, boxtrees, Abelia mosanensis T. H. Chung ex Nakai, and Selaginella stauntoniana Spring were abundant, as these are types of vegetation characteristic of limestone areas. Pinus densiflora S. et Z. was the most dominant woody plant, but vegetational changes, such as the dominance of Quercus variabilis Blume, Quercus dentata Thunb, and Quercus mongolica Fischer, were observed in spots. In particular, the growth of nursery plants of pinus densiflora S. et Z. was not observed, and young trees like Quercus mongolica Fischer grow as low vegetation of Pinus densiflora S. et Z.. Thus, the ecological succession of Quercus mongolica Fischer is predicted. Outside the investigation area, as herbaceous plants, various vegetations were observed, including Selaginella stauntoniana Spring, Thalictrum coreanum H. Lev., Mukdenia rossii(Oliv.) Koidz., Platanthera freynii Kraenzl., Cephalanthera longibracteata Blume, Potentilla dickinsii Franch., Patrinia rupestris(Pall.) Juss., Swertia pseudochinensis H. Hara, Vicia venosa(Willd.) Maxim., Pyrola japonica Klenze ex Alef., Disporum smilacinum A. Gray, Artemisia stolonifera(Maxim.) Kom. for. Stolonifera, Smilax nipponica Miq., Adenophora triphylla var. japonica(Regel) H. Hara, Isodon inflexus(Thunb.) Kudo, Gentiana scabra Bunge for. Scabra, Polygonatum odoratum var. pluriflorum(Miq.) Ohwi, Dioscorea quinqueloba Thunb., Syneilesis palmata(Thunb.) Maxim., Asparagus schoberioides Kunth, Eupatorium japonicum Thunb. ex Murray, Galium kinuta Nakai & Hara, Saxifraga fortunei var. incisolobata, Lilium amabile Palib., Siberian iris, Dictamnus dasycarpus Turcz., Atractylodes ovata(Thunb.) DC., and Lysimachia clethroides Duby.

• Plant Resources
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• v.1 no.1
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• pp.6-12
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• 1998

The Fp1 gene, originally isolated from red pepper seedlings, encode the iron storage protein, and have a high homology with ferritin genes at DNA and amino acid level. In order to determine ferritin protein expression in vegetative tissue. Fp1 gene was constructed in plant expression vector(PIG12IHm) and introduced in red pepper(var. Bukang, Chungyang and Kalag-Kimjang 2) via Agrobacterium tumefaciensmediated transformation. After selection on MS media containing Kanamycin(Km), putatively selected transformants were confirmed by amplification of selectable marker gene(Fp1 and NPII) by polymerase chain reaction. Northern blot showed that transcripts of Fp1 gene were detected in mature leaves of the plants. In A6, A7 and A8 and A14 of transgenic plants, transcript of Fp1 gene was increased seven-fold to eight-fold than other transgenic plants. Also the proteins obtained from leaves of transgenic plants were immunologically detected by Western blot using rabbit anti-ferritin polyclonal antibody. The expression protein appeared as strong band of apparent mass of 23.5kDa. suggesting the iron accumulation in transgenic red pepper plants.

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.213-218
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• 1999

Fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plants. In addition, it has been suggested that, due to their solubility, they can play an important role in helping plants survive periods of osmotic stress. In order to study the effect of levan synthesis on plant growth, the coding region of the levansucrase gene, which was isolated from Zymomonas mobilis, was introduced into tobacco plants using Agrobacterium tumefaciens-mediated transformation. The presence of the levansucrase gene in transgenic plants was verified by genomic DNA gel blot analysis. RNA gel blot and immunoblot analyses showed an accumulation of the corresponding transcript and protein product of the bacterial levansucrase gene in transgenic plants. Furthermore, a thin layer chromatography analysis revealed that fructans were synthesized and deposited in transgenic tobacco plants. When $T_1$ seeds were germinated and grown under polyethylene glycol-mediated drought stress or cold stress, the transgenic seedlings displayed a substantially higher level of growth than that of untransformed plants. These results suggest that fructans may playa significant role in the tolerance of plants under osmotic stress.

• Plant Biotechnology Reports
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• v.5 no.4
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• pp.323-329
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• 2011

MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

• Korean Journal of Pharmacognosy
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• v.23 no.4
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• pp.264-267
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• 1992

The cytotoxic activity of medicinal plants was screened using A549 human lung cancer cell line. Plant materials were extracted with 80% methanol and fractionated to chloroform and water layers. Each methanol, chloroform, and water extract of thirty-two medicinal plants was tested for cytotoxic activity in A549 cell culture system and the cell viability was measured by SRB assay.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.162-163
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• 2008

• Korean Journal of Oriental Medicine
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• v.14 no.1
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• pp.33-40
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• 2008

Revising 11 historical topography of Jecheon city we could notice that this territory was traditionally producing high quality medicinal plants. This view is supported as it was tributed to the King. Production of medicinal plants continue these days, Jecheon being one of the largest production of medicinal plants in Korea. Abundant production of diverse medicinal plants of high quality seems to be linked with the soil, climate and environmental characteristics unique of Jecheon. Therefore we propose to develop new type of herbal medicin drugs like Gobang(膏方) which could distinguish from other places. Gobang matchs well to the requirement of current days being convenient to carry and of good taste. Also is appropriate to treat chronic diseases. Its primordial abundant herbal medicines of high quality for the development of Gobang where Jecheon can provide.

• Journal of Korean Society for Atmospheric Environment
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• v.15 no.E
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• pp.45-54
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• 1999

The effectiveness of NOx emission reductions from power plants to alleviate persistent ozone nonattainment in the esterm Unites States was investigated with a focus on the Northeast Corridor, centered on New York City. The 1995 ozone episode along with the 2007 base case emission scenario was used with the Variable-Grid Urban Airshed Model(UAM-V) to determine ozone concentrations. Several scenarios based on EPA's proposal issued on October 10, 1997 were examined. Although it is widely recognized that the eastern United States includes a large Nox-sensitive region(e.g., Sillman, 1999), the study revealed that reducing NOx emissions from power plants beyond 500 miles (800km) was not effective for reducing ozone exceedances in the region. It was also found that NOx emissions from power plants play an important role in local ozone exceedances.