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http://dx.doi.org/10.1007/s11816-011-0186-z

Cadmium resistance in tobacco plants expressing the MuSI gene  

Kim, Young-Nam (Department of Environmental Horticulture, The University of Seoul)
Kim, Ji-Seoung (Department of Environmental Horticulture, The University of Seoul)
Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul)
Lee, Young-Woo (Department of Environmental Horticulture, The University of Seoul)
Baek, Seung-Woo (Department of Environmental Horticulture, The University of Seoul)
Kim, Il-Sup (Department of Biology, Kyungpook National University)
Yoon, Ho-Sung (Department of Biology, Kyungpook National University)
Kim, Kwon-Rae (Han-River Environment Research Center, National Institute of Environmental Research)
Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul)
Kim, Kye-Hoon (Department of Environmental Horticulture, The University of Seoul)
Publication Information
Plant Biotechnology Reports / v.5, no.4, 2011 , pp. 323-329 More about this Journal
Abstract
MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.
Keywords
MuSI; Cadmium tolerance; Rubber elongation factor (REF); Tobacco; Cadmium accumulation; Trichomes;
Citations & Related Records
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