Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Cadmium resistance in tobacco plants expressing the MuSI gene

  • Kim, Young-Nam (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Ji-Seoung (Department of Environmental Horticulture, The University of Seoul) ;
  • Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul) ;
  • Lee, Young-Woo (Department of Environmental Horticulture, The University of Seoul) ;
  • Baek, Seung-Woo (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Il-Sup (Department of Biology, Kyungpook National University) ;
  • Yoon, Ho-Sung (Department of Biology, Kyungpook National University) ;
  • Kim, Kwon-Rae (Han-River Environment Research Center, National Institute of Environmental Research) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Kye-Hoon (Department of Environmental Horticulture, The University of Seoul)
  • Received : 2011.02.05
  • Accepted : 2011.05.24
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

Keywords

References

  1. Cherian S, Oliveira MM (2005) Transgenic plants in phytoremediation: recent advance and new possibilities. Environ Sci Technol 39(24):9377-9390 https://doi.org/10.1021/es051134l
  2. Choi YE, Harada E, Wada M, Tsuboi H, Morita Y, Kusano T, Sano H (2001) Detoxification of cadmium in tobacco plants: formation and active excretion of crystals containing cadmium and calcium through trichomes. Planta 213:45-50 https://doi.org/10.1007/s004250000487
  3. Choi YE, Harada E, Kim GH, Yoon ES, Sano H (2004) Distribution of elements on tobacco trichomes and leaves under cadmium and sodium stresses. Plant Biol 47(2):75-82 https://doi.org/10.1007/BF03030635
  4. Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825-832 https://doi.org/10.1104/pp.123.3.825
  5. Cresser MS, Parsons JW (1979) Sulfuric-perchloric acid digestion of plant material for the determination of nitrogen, phosphorus, potassium, calcium and magnesium. Anal Chem Acta 109:431-436 https://doi.org/10.1016/S0003-2670(01)84273-2
  6. Das P, Samantaray S, Rout GR (1997) Studies on cadmium toxicity in plants: a review. Environ Poll 98:29-36 https://doi.org/10.1016/S0269-7491(97)00110-3
  7. Elmayan T, Tepfer M (1994) Synthesis of a bifunctional metallothionein/$\beta$-glucuronidase fusion protein in transgenic tobacco plants as a means of reducing leaf cadmium levels. Plant J 6:433-440 https://doi.org/10.1046/j.1365-313X.1994.06030433.x
  8. Espen S, D'Souza SF (2005) Prospects of genetic engineering of plants for phytoremediation of toxic metals. Biotechnol Adv 23:97-114 https://doi.org/10.1016/j.biotechadv.2004.10.001
  9. Kahel H (1993) Response of roots of trees to heavy metals. Environ Exp Bot 33:99-119 https://doi.org/10.1016/0098-8472(93)90059-O
  10. Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137-138
  11. Kawashima CG, Noji M, Nakamura M, Ogra Y, Suzuki KT, Saito K (2004) Heavy metal tolerance of transgenic tobacco plants over-expressing cysteine synthase. Biotechnol Lett 26:153-157 https://doi.org/10.1023/B:BILE.0000012895.60773.ff
  12. Kim KW (2008) Visualization of micromorphology of leaf epicuticular waxes of the rubber tree Ficus elastic by electron microscopy. Micron 39:976-984 https://doi.org/10.1016/j.micron.2007.10.006
  13. Kim SH, Song WK, Kim YH, Kwon SY, Lee HS, Lee IC, Kwak SS (2009) Characterization of full-length enriched expressed sequence tags of dehydration-treated white fibrous roots of sweetpotato. J Biochem Mol Biol 42(5):271-276
  14. Kim EY, Seo YS, Lee H, Kim WT (2010) Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants. Planta 232:71-83 https://doi.org/10.1007/s00425-010-1149-2
  15. Lee JH, Bae HJ, Jeong JY, Lee JY, Yang YY, Hwang IH, Martinoia E, Lee YS (2003) Functional expression of a bacterial heavy metal transporter in Arabidopsis enhances resistance to and decreases uptake of heavy metals. Plant Physiol 133:589-596 https://doi.org/10.1104/pp.103.021972
  16. Maiti IB, Wagner GJ, Yeargan R, Hunt AG (1989) Inheritance and expression of the mouse metallothionein gene in tobacco. Plant Physiol 91:1020-1024 https://doi.org/10.1104/pp.91.3.1020
  17. Oh SK, Kang HS, Shin DH, Yang JM, Chow KS, Yeang HY, Wagner B, Breiteneder H, Han KH (1999) Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem 274:17132-17138 https://doi.org/10.1074/jbc.274.24.17132
  18. Pan A, Tie F, Duau Z, Yang M, Wang Z, Li L, Chen Z, Ru B (1994) $\alpha$-Domain of human metallothionein $I_A$ can bind to metals in transgenic tobacco plants. Mol Gen Genet 242:666-674
  19. Robinson BH, Mills TM, Petit D, Fung LE, Green SR, Clothier BE (2000) Natural and induced cadmium-accumulation in poplar and willow: implications for phytoremediation. Plant Soil 227:301-306 https://doi.org/10.1023/A:1026515007319
  20. Salt DE, Prince RC, Pickering IJ, Raskin I (1995) Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiol 109:1427-1433
  21. Seo SG, Kim JS, Yang YS, Jun BK, Kang SW, Lee GP, Kim W, Kim JB, Lee HU, Kim SH (2010) Cloning and characterization of the new multiple stress responsible gene I (MuSI) from sweet potato. Genes Genom 32:552-554
  22. Song WY, Sohn EJ, Martinoia E, Lee YJ, Yang YY, Jasinski M, Forestier C, Hwang I, Lee YS (2003) Engineering tolerance and accumulation of lead and cadmium in transgenic plants. Nat Biotechnol 21:914-919 https://doi.org/10.1038/nbt850
  23. Steyn WJA (1959) Leaf analysis. Errors involved in the preparative phase. J Agric Food Chem 7:344-348 https://doi.org/10.1021/jf60099a007
  24. Welch RM, Norvell WA (1999) Mechanisms of cadmium uptake, translocation and deposition in plants. In: McLaughlin MJ, Singh BR (eds) Cadmium in soils and plant. Kluwer, Dordrecht, pp 125-150
  25. Westerman RL (1990) Soil testing and plant analysis, 3rd edn. SSSA book series, no. 3, pp 389-427
  26. Yamazaki K (1982) Nutrient solution culture (Japanese). Pak-kyo, Tokyo, p 251
  27. Zenk MH (1996) Heavy metal detoxification in higher plants. Gene 179:21-30 https://doi.org/10.1016/S0378-1119(96)00422-2
  28. Zhu YL, Pilon-Smits EAH, Jouanin L, Terry N (1999) Overexpression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance. Plant Physiol 119:73-79 https://doi.org/10.1104/pp.119.1.73

Cited by

  1. 토양 중 고농도 카드뮴에 노출된 MuS1 형질전환 담배 (Nicotiana tabacum cv. Xanthi)의 생리적 반응 및 카드뮴 축적: 식물학적 오염토양정화를 위한 형질전환 식물 탐색 vol.46, pp.1, 2011, https://doi.org/10.7745/kjssf.2013.46.1.058
  2. Floral Nectary Morphology and Proteomic Analysis of Nectar of Liriodendron tulipifera Linn. vol.7, pp.None, 2011, https://doi.org/10.3389/fpls.2016.00826
  3. Phytoextraction of Heavy Metals: A Promising Tool for Clean-Up of Polluted Environment? vol.9, pp.None, 2018, https://doi.org/10.3389/fpls.2018.01476
  4. Evaluation of Dittrichia viscosa performance in substrates with moderately low levels of As and Cd contamination vol.154, pp.6, 2020, https://doi.org/10.1080/11263504.2020.1836061
  5. The Potential Application of Giant Reed (Arundo donax) in Ecological Remediation vol.9, pp.None, 2011, https://doi.org/10.3389/fenvs.2021.652367