• 한국환경과학회지
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• 제30권12호
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• pp.1041-1051
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• 2021

The purpose of this study was to identify for extenders, adhesives, colorants, and fungicides suitable for the production of high-quality film-coated seeds of Chinese cabbage. In addition, the effect of various additives used for film coating on the germination of coated seeds and seedling vigor was investigated to establish appropriate treatment conditions for film coating efficiency. The germination rate and germination speed in Chinese cabbage film-coated seeds did not differ significantly depending on the type and concentration of the extender, while the use of Talc resulted in superior granulation capacity and adhesion of the seed coat in comparison to Zeolite. There was no significant difference in the germination rate depending on the type of adhesive used for film coating of Chinese cabbage seeds, but the germination rate was slightly slower in the film-coated seeds using carboxymethyl cellulose as an adhesive. On the other hand, the seeds coated with a film using 1% PVA as an adhesive not only maintained hardness enough to withstand external pressure, but also showed a high germination rate. In addition, the suitable disinfectant for film coating was venoram, and the treatment concentration was good at 250 mg·L^-1. The type of colorant used in the film-coated seeds of Chinese cabbage did not significantly affect the germination rate, germination speed, and seedling vigor. The seeds film-coated using the liquid colorant were superior in appearance to the seeds film-coated with the solid colorant, which implies the possibility of reduction in the manufacturing costs by using the water-soluble paints as colorants for film-coated seeds.

• 한국미생물·생명공학회지
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• 제17권4호
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• pp.403-406
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• 1989

To establish tile standard condition of uniformed Kimchi product, we introduced the concept of starter and studied the preparation of rapid-fermented Kimchi. Of the strains isolated from Kimchi, Kakdugi and infant's feces, M7 strain grew effectively on aseptic Chinese cabbage juice and on salted Chinese cabbage, the growth of M7 was decreased severely. Inoculated with M7 in salted Chinese cabbage, appropriate range of pH and lactic acid content were reached at 8-13 hrs and 12 hrs after addition of spices, respectively. The result of sensory evaluation was not significant at 5% level.

• Animal cells and systems
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• 제9권4호
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• pp.239-244
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• 2005

A cDNA clone for a wound- and pathogen-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene, designated BrPR4, encodes a pathogenesis-related protein 4 (PR4) of 140 amino acids. The BrPR4 protein shows high similarity with wound-inducible antifungal proteins of tobacco, potato, barley, and wheat. The BrPR4 gene is locally induced by a nonhost pathogen, Pseudomonas syringae pv. tomato, that elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with benzothiadiazole (BTH), methyl jasmonate or ethephon showed that the BrPR4 gene expression is strongly induced by ethylene, but not by methyl jasmonate or BTH. The BrPR4 gene is also activated by wounding. Interestingly, however, the wound-inducible BrPR4 gene expression is repressed by salicylic acid or BTH, suggesting that there is cross-talk between salicylate-dependent and -independent signaling pathways.

• 한국토양비료학회지
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• 제50권1호
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• pp.49-55
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• 2017

Chinese cabbage cultivation and ammonia volatilization experiments were done to evaluate the efficiency of Agrotain coated urea (GSP 80% + Agro; GSP 100% + Agro) against conventional urea (GSP 80%; GSP 100%). Fresh weight of Chinese cabbage were 17.2% and 7.3% higher in the treatments that received GSP 80% + Agro and GSP 100% + Agro, respectively, of those from the treatments that received urea alone. Likewise, the nitrogen use efficiency of Chinese cabbage in the treatments that received Agrotain coated urea were significantly higher at the rate of 3.5% (GSP 80% + Agro) and 1.9% (GSP 100% + Agro) compared to urea alone treatments. Ammonia emission was substantially higher at the rate of $107.6N\;mg\;chamber^{-1}$ with the application of only GSP 100%. However, nearly 28.3% of ammonia emission was considerably reduced with the use of Agrotain coated urea. Hence, we recommend the use of Agrotain coated urea in conventional farming for increased crop yield as well as simultaneous reduction of nitrogenous fertilizer use.

• 원예과학기술지
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• 제28권4호
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• pp.593-599
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• 2010

6-7월, 8-9월, 10-11월, 12-4월(2007년) 및 5월(2008년)에 수확한 신선배추, 절임공정 및 김치 제조과정을 거친 배추를 겉잎과 속잎 부위로 나누어 anion-exchange column을 통과시키고 아세톤 침전법을 사용하여 제조한crude myrosinase로 반응시켜 total glucosinolates 함량을 분석하였다. 신선배추의 경우 6-7월 신선배추의 total glucosinolates 함량이 가장 높은 함량을 나타내었으며, 모든 수확기간에서 속잎보다 겉잎이 대체로 높은 것으로 나타났다. 가공처리 한 배추의 total glucosinolates 함량을 분석 한 결과, 절임배추의 경우 8-9월 절임배추에서 가장 높게 나타났으며, 5월 절임배추의 경우 다른 수확기간에 비해 낮은 함량인 것으로 분석되었다. 부위 별 절임배추의 total glucosinolates 함량은 6-7월 절임배추와 8-9월 절임배추의 경우 겉잎의 함량이 속잎의 함량 보다 높은 것으로 분석된 반면, 10-11월, 12-4월 및 5월 절임배추에서는 속잎의 함량이 높은 것으로 확인 되었다. 배추김치의 경우 6-7월, 8-9월 김치의 total glucosinolates 함량은 비교적 높은 함량을 나타내었으며, 5월 김치의 경우 다른 수확시기에 비해 낮은 함량인 것으로 측정되었다. 배추김치의 부위별 total glucosinolates함량은 5월 배추김치의 경우 속잎의 함량이 겉잎의 함량보다 높게 분석된 반면, 6-7월, 8-9월, 10-11월 배추김치의 경우 겉잎의 함량이 속잎의 함량보다 높은 것으로 분석되었다. 모든 수확기간에서 신선배추의 total glucosinolates 함량보다 가공 처리한 절임배추, 배추김치의 total glucosinolates 함량이 감소하는 경향을 볼 수 있었다. 이러한 분석 결과는 배추의 total glucosinolates 함량이 수확기간, 부위 및 가공처리에 따라 영향을 받는 것으로 사료되며, total glucosinolates 함량에 영향을 줄 수 있는 배추의 부위, 품종, 재배방법 및 재배환경(pH, 온도, 기후, 토양 등)은 고려 되어야 한다고 판단된다.

• Plant Biotechnology Reports
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• 제1권1호
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• pp.19-25
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• 2007

To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2-3 leaf stage were most vulnerable to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the bar gene in T₁ segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions. However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for genetically modified (GM) Chinese cabbage cultivars.

• 식물병연구
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• 제9권1호
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• pp.39-41
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• 2003

Erwinia carotovora subsp. carotovora에 의한 배추 무름병은 배추에서 가장 큰 문제가 되고 있는 병중 하나이다. 본 연구는 배추 품종별 무름병 저항성을 조기에 검정하고자 실시하였다. 선발된 균주를 이용하여 세균현탁액과 mineral oil을 4:1로 혼합하여 배추의 중앙 기부에 10m1 관주 접종하는 mineral oil 접종법으로 접종하였다. 국내의 3개 종묘회사와 대학으로부터 분양 받은 43개의 배추품종 및 계통의 저항성 정도를 조사하였다. 그 결과 C3-26, C3-28, C3-29, C29-51-51-52는 저항성, 강타배추, 금촌얼갈이, 미니배추, 금빛, DB50, 지부, 평층, 산촌배추는 감수성인 것으로 나타났으며, 나머지는 중간정도의 저항성을 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.466-470
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• 1998

The basic and optimum conditions for the extraction of peroxidase from Chinese cabbage root applying the reverse micelle system were investigated. In order to purify Peroxidase (POX) from crude extract of Chinese cabbage roots, isooctane containing 110 mM Aerosol OT (AOT) was well mixed with the same volume of crude extracts containing 50 mM NaCl and 30 mM Tris-HCI buffer of pH 8.0. After centrifugation, AOT reverse micelle containing the isooctane phase were mixed with 80 mM Tris-HCI buffer at pH 7.0 containing 1 M KCl. From these operations, POX was purified 20-fold with a 60% yield. For further purification, DEAE-Toyopearl column chromatography was applied, and it showed a single protein band on SDS-polyacrylamide gel electrophoresis. The resulting POX showed 93-fold purification with a 40% yield.

• Preventive Nutrition and Food Science
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• 제4권2호
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• pp.113-116
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• 1999

The anticancer effect of methanol extracts from common Chinese cabbage kimchi(CC kimchi ) and organically cultivated Chinese cabbage kimchi (OC kimchi) was studied on the cell growth, MTT assay and SRB assay using AGS human gastric cancer cells. Methanol extracts from CC kimchi and OC kimchi exhibited the anticancer activites in vitro and in vivo. Methanol extract from 6 day-fermented CC kimchi and OC kimchi inhibited the growth of AGS cells by 55.2 and 60.7% , respectively. At MTT assay an dSRB assay, 6 day-fermented OC kimchi showed higher inhibition rate (MTT : 42%, SRB : 61%) than 6 day-fermented CC kimchi(MTT : 33%, SRB : 52%). Methanol extracts from 6-day fermented CC kimchi and OC reduced the tumor formation and prolonged the life span of sarcoma-180 cell injected Balb.c mouse. OC kimchi treated group resulted in the smaller tumor weight of 4.58$\pm$0.32g compared th the CC kimchi group of 5.40$\pm$0.78g and the control group of 7.50$\pm$0.54g and OC kimchi treted group (25.3 days) lived longest among control (20.2days ) and CC kimchi(23.5days) treted groups.

• 한국토양비료학회지
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• 제35권2호
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• pp.105-111
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• 2002

현재 채소류에 대한 질소시비량 추천식은 육지부토양에서 시험한결과를 이용하여 설정된 것으로 이를 화산회토양에서도 활용하는 문제가 있어 이를 검토하기위하여 제주도 토양을 중심으로 1999년에는 pot와 2000년에는 포장에서 몇개의 질소시비량수준을 두고 배추를 재배한 시험결과는 다음과 같다. 1. Pot와 포장시험에서 배추의 질소 최고시비량은 $294{\sim}331kg\;ha^{-1}$이었고 토양의 유기물함량과 최고시비량과의 관계를 이용하여 화산회토양에 알맞는 새로 질소시 비량 추천식을 설정하였다. 2. 배추의 질소시비량시험을 통한 화산회토의 실제 질소시비적량은 기존의 추천식을 이용한 시비량과는 큰차이를 보였고 개선된 질소시비 추천식으로부터 산출된 질소 시비량과는 근소한 차이를 보였다. 3. 수화기 T-N 함량 및 질소흡수량은 질소시비량이 증가할수록 많았으나 질소이용 율은 질소시비량이 증가할수록 낮아지는 경향이었다. 4. 화산회토양에서 재배하는 양배추 대한 토양유기물함량에 따른 질소 시비량은 배추대비 질소흡수량을 흡수량비교 계수로 하고 이를 이용하여 양배추의 새로운 질소시비 추천식을 도출하였다.