• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1946-1954
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• 2018

The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of ($11.72{\pm}0.03$) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4-11) and temperature ($4-50^{\circ}C$). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and $22^{\circ}C$ for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.

• 복식
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• 제5권
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• pp.173-183
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• 1981

In 1895(32nd year of King Ko-Jong), Kyung-moo-chung as westernized police system was established and the uniform of the police developed. Regarding to the Uniform of the Kyung-bu-dai-sin(the Minister Kyung-bu) and to that of the Sun-gum(the low position of the Kyung-bu police), the Kyung-bu costume(Uniform of the police) is divided as Ye-bok(Formal uniform) and Sang-bok(Informal uniform). Ye-bok(Formal uniform) is put on in the case of the law described, and Sang-bok(Informal uniform) is an everyday wear. Ye-bok(Formal uniform) is composed of Ye-mo(hat), Eui(jacket), Go(trousers), Dai(decorative belt), Do(decorative sword), and Hwa(footwear). Sang-bok(Informal uniform) is composed of Sang-mo(hat), Eui(jacket), Go(trousers), and Hwa(footwear). Differences between Sang-bok(Informal uniform) and Ye-bok(Formal uniform) is the materials of the ornament, numbers of the decorative lines. Ye-bok(Formal uniform) has more grandeur[ornament. A historic changes in the Kyung-bu costume(Uniform of the police) during the late of Yi Dynasty was little but the decorative materials and the numbers of the decorative line.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.61-69
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• 2001

A total of 120 pigs were used to investigate the effects of yucca extracts on the growth performance, nutrient digestibility and excretion of growing pigs fed different levels of dietary protein. Pigs were allotted into $2{\times}3$ factorial design by the supplementation with yucca extract (YE, 0 and 120 mg/kg) and 3 levels of dietary protein (16, 18, 20%). During the whole experimental period (18 to 52 kg body weight), there were no significant differences in ADG, ADFI or F/G by YE addition or different protein levels among treatments (p>0.05). Overall, although addition of YE to the diet and elevation of protein level showed better ADG, there were no significant differences in growth performance among treatments. Pigs fed diets with YE showed significantly (p<0.05) higher dry matter (DM), crude ash (CA) and crude protein (CP) digestibility than did the others during the growing period. Concerning the levels of dietary protein, only the CP digestibility was significantly higher in pigs fed high protein diet. Pig fed the low protein diet without YE showed a significantly low CP digestibility (p<0.05). No significant differences were found in crude fat (CF), calcium (Ca) and phosphorus (P) digestibilities regardless of YE supplementation or dietary protein levels. Pigs fed YE supplemented diets showed significantly (p<0.05) higher amino acid digestibility. Also, high CP level diets showed a higher amino acid digestibility than low CP diets (p<0.05). DM and N excretion did not show any significant differences among treatments, there was a slightly lower excretion with increase in dietary protein level. Supplementation with YE significantly decreased the DM and N excretion. Interaction (YE$\times$protein) was found in P excretion. Pigs fed a medium protein diet without YE showed the lowest P excretion during the growing period. The NH3-N content in the feces tended to be increased by the increased dietary protein levels and with YE supplementation. During the whole experimental period, the cost for YE supplementation was similar to value of the improvements of performance obtained. The cost of feeding high level protein was significantly higher than that of medium level protein by 10% and low level protein by 9% (p<0.05). It could be concluded that the effects of dietary protein level and yucca extract on growth performance, nutrient digestibility and excretion might play a role to some extent in growing pigs from the aspect of pollution control.

• 건축사
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• 통권601호
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• pp.72-79
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• 2019

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.525-534
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• 2001

A total of 120 pigs were used to investigate the effects of yucca extracts on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics of finishing pigs fed different levels of dietary protein. Pigs were allotted into $2{\times}3$ factorial design by the supplementation of yucca extracts (YE, 0 and 120 mg/kg) and 3 levels of dietary protein (14, 16, 18% for early finisher and 12, 14, 16% for late finisher for low, medium and high protein diet, respectively). During the early finishing period (51~76 kg BW), no significant difference was found in growth performance regardless of the YE supplementation or dietary protein levels. Growth performance of late finishing pigs (76~101 kg BW) was also not significantly different among treatments. However, ADG of pigs fed YE diet was significantly improved (p<0.05) regardless of the dietary protein levels. For the overall period (51~101 kg BW), although adding YE to the diet and elevating the protein level showed better ADG, there were no significant differences on growth performance among treatments. Early finishers showed significantly higher crude protein, crude ash and crude fat digestibilities when they were fed diets supplemented with YE. Digestibilities of amino acids were not affected by YE. Late finishers did not show any significant differences in proximate nutrient digestibilities regardless of YE supplementation or dietary protein levels. YE tended to slightly improve the CP digestibility, however no significant difference was found with increased dietary protein levels. There was no significant difference in amino acid digestibilities with YE supplementation or dietary CP levels during the late finishing period. Dry matter (DM) and nitrogen (N) excretion in feces did not show any significant difference among treatments. Early finishing pigs also did not respond to the inclusion of YE or dietary protein levels (p<0.05). Fecal N excretion of early finishing pigs seemed to be lowered in pigs fed YE. Pigs fed medium dietary protein diet tended to excrete a higher amount of N during the early finishing period, but not statistically different. A slight increase in fecal N excretion was found with the increased level of dietary protein during the late finishing period. For ammonia nitrogen excretion, although there was no significance, the NH3-N content tended to be increased by the increased dietary protein levels and with YE supplementation. The NH3-N content in manure increased by 24.5% with YE supplementation. There were no significant differences in carcass weight, backfat thickness, carcass grade and loin eye area among treatments. However, pigs fed non-YE with low protein diet showed a significantly (p<0.05) low carcass ratio among treatments and there was significant (p<0.05) difference between the YE-added treatment and non YE treatment in carcass ratio. As for the feed cost, the cost of feeding high level protein was higher than that of medium level protein by 5% and low level protein by 9% (p<0.05). Therefore, based on this study, it could be concluded that environmentally friendly agents might play a role to some extent in finishing pigs from the aspect of pollution control, and that more than 14 and 12% of dietary protein for early finishing and late finishing pigs respectively do not necessarily guarantee high growth performance.

• 생명과학회지
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• 제9권6호
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• pp.653-658
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• 1999

A novel marine microorganism, Vibrio sp. YE-101, was isolated from pufferfish and investigated for its ability to synthesize tetrodotoxin (TTX). Various strains isolated from the intestine of pufferfish were grown on TCBS agar plate, and then cultured on Ocean Research Institute (ORI) medium supplemented with 3% NaCl at 23$^{\circ}C$ for 3days. The cells were harvested, disrupted, fractionated by Bio-Gel P-2 column chromatography and then TTX-producing strain, Vibrio sp. YE-101, was identified using mouse bioassay. The isolated TTX from Vibrio sp. YE-101 was also analyzed and identified by HPLC and gas chromatography-mass spectrometer (GC-MS). The mass fragmentation of trimethylsilyl derivatives of C9-base of TTX from Vibrio sp. YE-101 was interpreted and the pattern of fragmentation was same with that of authentic standard. The purfied TTX was also positive to the mouse bioassay, which clearly represents that Vibrio sp. YE-101 can synthesize TTX.

• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.1976-1982
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• 2007

In the current studies, we characterized the degradation of a hot mixture of benzene and toluene (BT) gases by a thermophilic biofilter using polyurethane as a packing material and high-temperature compost as a microbial source. We also examined the effect of supplementing the biofilter with yeast extract (YE). We found that YE substantially enhanced microbial activity in the thermophilic biofilter. The degrading activity of the biofilter supplied with YE was stable during long-term operation (approximately 100 d) without accumulating excess biomass. The maximum elimination capacity ($1,650\;g{\cdot} m^{-3}{\cdot} h^{-1}$) in the biofilter supplemented with YE was 3.5 times higher than that in the biofilter without YE ($470\;g{\cdot} m^{-3}{\cdot} h^{-1}$). At similar retention times, the capacity to eliminate BT for the YE-supplemented biofilter was higher than for previously reported mesophilic biofilters. Thus, thermophilic biofiltration can be used to degrade hydrophobic compounds such as a BT mixture. Finally, 168 rDNA polymerase chain reaction-DGGE (PCR-DGGE) fingerprinting revealed that the thermophilic bacteria in the biofilter included Rubrobacter sp. and Mycobacterium sp.

• 한국식품과학회지
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• 제29권5호
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• pp.839-846
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• 1997

Meat-like flavor를 생성하고자 당과 아미노산 혼합액인 0.2 M cystine-0.1 M lactose-0.1 M maltose를 대조구로 하여 천연 조미향상물질(HVP, HAP, YE)을 첨가하였다. 천연 조미향상물질의 첨가는 대조구에 농도별로 단독 첨가하였으며 단독 첨가시 선정된 농도의 HVP, HAP나 YE를 혼합하여 그 영향을 비교하였다. Meat-like flavor 생성에 유사한 조건인 $100^{\circ}C$에서 8시간 동안 마이야르 반응시킨 반응액은 흡광도, 색, 관능적 성질 및 휘발성 향기성분을 분석하였다. 그 결과 HVP, HAP, YE를 첨가 농도별로 첨가하였을 때 반응 시간과 첨가 농도가 증가할수록 420 nm와 278 nm에서의 흡광도가 증가하였다. 또한 첨가 농도별 색의 변화는 HVP, HAP와 YE 모두 b값은 증가 경향을 나타낸 반면 L값은 감소 경향을 나타내었다. 관능 검사에서 meat flavor에 근접한 각 조미향상물질의 첨가 농도는 1.16% HVP, 0.94% HAP, 1.48% YE이었고 가장 meat flavor에 근접한 반응액은 대조구에 HVP 1.16%와 HAP 0.94%를 혼합 첨가한 반응액이었다. 대조구와 HVP 단독 첨가구 및 HVP와 HAP 혼합 첨가구에 대한 향기 성분을 비교하고자 GC/MS로 분석한 결과 hydrocarbon류 1종, aldehyde류 9종, ketone류 5종, ester류 1종, alcohol류 5종, aromatics(benzene)류 2종, furan류 2종, sulfur compound류 1종을 확인할 수 있었다.

• 동양고전연구
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• 제35호
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• pp.161-188
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• 2009

유학 특히 주자 성리학의 예(禮)는 절대적 보편적 이치로서 천이(天理)와 상대적 관계적 규범 형식으로서 절문(節文)과 의칙(儀則)으로 개념 규정된다. 예의 본질은 이렇게 두 가지 의미로 구분되는데, 절대성과 상대성이 중층적으로 혼합되어 있지만 단순한 양가성(兩價性)으로 해석되어서는 곤란하다. 예가 발현되는 과정에는 천리(天理)와 인성(人性)이라는 본질 외에 시간(時間)과 공간(空間)이라는 일상성(日常性)이 매개되기 때문이다. 따라서 양자가 서로 조화를 이루었을 때 예(禮)의 현실적 효용이 극대화될 수 있다. 물론 예(禮)는 구분의 원리이고, 악(樂)은 조화의 기능을 수행하여 상호보완적 역할을 하는 측면도 없지 않다. 그러나 예의 본질은 신분, 계급, 귀천 등 현실적 차이 속에서도 쌍무호혜적(雙務互惠的) 성격을 특징으로 한다. 유학은 일반인은 물론 최고 통치자에게도 엄격한 도덕성과 윤리를 요구하며 이를 상실한 인군(人君)은 일개 필부(匹夫)에 불과한 것으로 간주한다. 예를 제정하는 주체가 통치자가 아니라 성인(聖人)인 것도 동일한 맥락에서이다. 예의 현실적 효용은 '예교(禮敎)'를 실현하는 것이다. 예교는 예의 본질과 일상성이 발현되는 개인적 사회적 정치적 기제이다. 구득된 인간 본성의 발현, 주체와 타자의 소통을 매개하는 문화의 형성, 윤리 도덕적 규범에 기초한 정치행위 등이 활발한 사회가 예교가 지향하는 유교적 공동체의 본모습이다. 유교적 공동체의 원리로서 예교의 특징을 민본성(民本性)과 시의성(時宜性)을 중심으로 고찰한 이유는 예의 본질이 구현되는 일상성(日常性)의 측면에 착안한 것이다. 이는 유교가 지닌 본원적 특질인 동시에 천리(天理)와 인성(人性) 자체에 대한 원리적 탐구에 머물지 않고 인정(人情)과 시속(時俗)이라는 현실을 실천의 장으로 고민하는 실학적(實學的) 성격인 것이다. 16, 7세기를 거치며 조선사회에서는 예의 일상화(日常化)가 본격적으로 진행되는데, 세종조 이후 삼강행실도(三綱行實圖)가 지속적으로 보급되어 도덕수신서(道德修身書)로 활용되었고 도덕성과 호혜성에 기반한 향촌자치규약인 향약(鄕約)이 보급 시행되었다. 율곡 이이는 중국의 여씨향약(呂氏鄕約)을 전범으로 하여 해주향약(海州鄕約)을 완성하였는데, 예교의 본질을 일상에서 실현하고자 하는 실학적 특성으로 평가할 수 있다.

• 대한환경공학회지
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• 제32권5호
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• pp.509-516
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• 2010

토양으로부터 농화배양된 두 미생물 군집의 디젤 분해 특성과 미생물의 군집 양상을 살펴보았다. 간균 형태를 띄는 두 군집은 육안으로 뚜렷히 구별되는 황색(YE-5)과 투명한 형태(WH-5)의 콜로니를 형성하였으며 1% 디젤 오염된 배지에서 26일간 배양하였을 때 디젤 분해율은 99.07 mg-Diesel/$L{\cdot}day$와 57.82 mg-Diesel/$L{\cdot}day$로 YE-5가 약 1.7배정도의 빠른 분해속도를 나타내었다. YE-5에 의한 디젤의 분해양상은 $C_8-C_{24}$ 전반에 걸쳐고르게 분해되는 양상을 보여주었다. PCR-DGGE 기법을 이용하여 YE-5를 동정한 결과 Psedomonas, Klebsiella, Escherichia, Stenotrophomonas 등이 관찰 되었으며 모두 단백세균에 속하는 것으로 분석 되었고 YE-5에서만 Uncultured Stenotrophomonas sp.가 관찰되었다. 본 실험을 통해 디젤의 효과적 분해를 위해 적절한 군집의 조합이 필요하다는 것을 알 수 있었으며 Escherichia hermannii나 Uncultured Stenotrophomonas sp.와 같은 기 보고되지 않은 종들이 디젤 분해에 미치는 영향에 관한 후속연구가 필요할 것으로 판단하였다.