Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Identification of Tetrodotoxin-producing Marine Microorganism from Pufferfish

복어로부터 복어독(Tetrodotoxin) 생성능이 있는 해양 미생물의 분리 및 동정

  • 윤성준 (경성대학교 공과대학 식품공학과) ;
  • 차병윤 (경성대학교 공과대학 식품공학과) ;
  • 이명자 (서울식품의약품안전청 시험분석실) ;
  • 정동윤 (서울식품의약품안전청 시험분석실) ;
  • 송병권 (부산식품의약품안전청 시험분석실) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 김동수 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Published : 1999.12.01
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Abstract

A novel marine microorganism, Vibrio sp. YE-101, was isolated from pufferfish and investigated for its ability to synthesize tetrodotoxin (TTX). Various strains isolated from the intestine of pufferfish were grown on TCBS agar plate, and then cultured on Ocean Research Institute (ORI) medium supplemented with 3% NaCl at 23$^{\circ}C$ for 3days. The cells were harvested, disrupted, fractionated by Bio-Gel P-2 column chromatography and then TTX-producing strain, Vibrio sp. YE-101, was identified using mouse bioassay. The isolated TTX from Vibrio sp. YE-101 was also analyzed and identified by HPLC and gas chromatography-mass spectrometer (GC-MS). The mass fragmentation of trimethylsilyl derivatives of C9-base of TTX from Vibrio sp. YE-101 was interpreted and the pattern of fragmentation was same with that of authentic standard. The purfied TTX was also positive to the mouse bioassay, which clearly represents that Vibrio sp. YE-101 can synthesize TTX.

Keywords

References

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