• Molecules and Cells
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• 제37권1호
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• pp.66-73
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• 2014

Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-${\alpha}$/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatment-naive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ${\zeta}$ expression on $CD8^+$ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-$1^+$ cells were closely associated with the histological activity index in CHC. The TCR ${\zeta}$ chain was significantly downregulated on hepatic $CD8^+$ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ${\zeta}$ chain expression in $CD8^+$ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ${\zeta}$ chain expression.

• 대한한방부인과학회지
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• 제28권3호
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• pp.1-10
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• 2015

Objectives Diagnosis System of Oriental Medicine (DSOM) was made as a computerized assistant program for oriental medicine doctors to be able to diagnose with statistical basis. Then DSOM uses questionnaires filled out by respondents with explanatory guide. But if the respondents misunderstand the meaning of the passages, the results were quite the opposite. Methods This study was designed to investigate the diagnostic correspondent rates between DSOM and TKM practitioners. First, let the respondents answer to DSOM. After that, three doctors diagnosed the respondents and marked 'p' when they diagnose that the respondent had the pathogenic factors, marked 'n' when they diagnose that the respondent had the pathogenic factors but not severs, and did not marked when they diagnose that the respondent didn't have the pathogenic factors. Finally, this study was investigated the correspondent rates of diagnosis between DSOM and doctors. Results In the pathogenic factor of three including insufficiency of Yin (陰虛), the correspondent rates were 90%. In the pathogenic factor of nine including deficiency of qi (氣虛), the correspondent rates were 80%. In the pathogenic factor of four including blood stasis (血瘀), the correspondent rates were 70%. In HH and HL, they showed the correspondent rates of 61.77%. The correspondent rate of heat (熱) was highest (96.88%). The correspondent rate of insufficiency of Yang (陽虛) was lowest (0%). In LH and LL, they showed the correspondent rates of 88.31%. The correspondent rate of blood stasis (血瘀) was lowest (71.76%). They all showed the correspondent rates of over 70%. Conclusions In DSOM and Doctors' diagnose, they showed the correspondent rates of 83.60%.

• Animal Bioscience
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• 제34권4호
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• pp.680-691
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• 2021

Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens. Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by Candida tropicalis until 42 days old. In the experiment, the chickens of the control group were fed soybean meal. Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration. Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition.

• Journal of Ginseng Research
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• 제46권3호
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• pp.426-434
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• 2022

Aim: This study investigates the effects of ginsenoside Rb1 (GsRb1) on methamphetamine (METH)-induced toxicity in SH-SY5Y neuroblastoma cells and METH-induced conditioned place preference (CPP) in adult Sprague-Dawley rats. It also examines whether GsRb1 can regulate these effects through the NR2B/ERK/CREB/BDNF signaling pathways. Methods: SH-SY5Y cells were pretreated with GsRb1 (20 mM and 40 mM) for 1 h, followed by METH treatment (2 mM) for 24 h. Rats were treated with METH (2 mg/kg) or saline on alternating days for 10 days to allow CPP to be examined. GsRb1 (5, 10, and 20 mg/kg) was injected intraperitoneally 1 h before METH or saline. Western blot was used to examine the protein expression of NR2B, ERK, P-ERK, CREB, P-CREB, and BDNF in the SH-SY5Y cells and the rats' hippocampus, nucleus accumbens (NAc), and prefrontal cortex (PFC). Results: METH dose-dependently reduced the viability of SH-SY5Y cells. Pretreatment of cells with 40 µM of GsRb1 increased cell viability and reduced the expression of METH-induced NR2B, p-ERK, p-CREB and BDNF. GsRb1 also attenuated the expression of METH CPP in a dose-dependent manner in rats. Further, GsRb1 dose-dependently reduced the expression of METH-induced NR2B, p-ERK, p-CREB, and BDNF in the PFC, hippocampus, and NAc of rats. Conclusion: GsRb1 regulated METH-induced neurotoxicity in vitro and METH-induced CPP through the NR2B/ERK/CREB/BDNF regulatory pathway. GsRb1 could be a therapeutic target for treating METH-induced neurotoxicity or METH addiction.

• Smart Structures and Systems
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• 제29권4호
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• pp.625-640
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• 2022

Maglev rail joints are vital components serving as connections between the adjacent F-type rail sections in maglev guideway. Damage to maglev rail joints such as bolt looseness may result in rough suspension gap fluctuation, failure of suspension control, and even sudden clash between the electromagnets and F-type rail. The condition monitoring of maglev rail joints is therefore highly desirable to maintain safe operation of maglev. In this connection, an online damage detection approach based on three-dimensional (3D) convolutional neural network (CNN) and time-frequency characterization is developed for simultaneous detection of multiple damage of maglev rail joints in this paper. The training and testing data used for condition evaluation of maglev rail joints consist of two months of acceleration recordings, which were acquired in-situ from different rail joints by an integrated online monitoring system during a maglev train running on a test line. Short-time Fourier transform (STFT) method is applied to transform the raw monitoring data into time-frequency spectrograms (TFS). Three CNN architectures, i.e., small-sized CNN (S-CNN), middle-sized CNN (M-CNN), and large-sized CNN (L-CNN), are configured for trial calculation and the M-CNN model with excellent prediction accuracy and high computational efficiency is finally optioned for multiple damage detection of maglev rail joints. Results show that the rail joints in three different conditions (bolt-looseness-caused rail step, misalignment-caused lateral dislocation, and normal condition) are successfully identified by the proposed approach, even when using data collected from rail joints from which no data were used in the CNN training. The capability of the proposed method is further examined by using the data collected after the loosed bolts have been replaced. In addition, by comparison with the results of CNN using frequency spectrum and traditional neural network using TFS, the proposed TFS-CNN framework is proven more accurate and robust for multiple damage detection of maglev rail joints.

• Journal of Ginseng Research
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• 제47권1호
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• pp.106-116
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• 2023

Background: Pirarubicin (THP) is an anthracycline antibiotic used to treat various malignancies in humans. The clinical usefulness of THP is unfortunately limited by its dose-related cardiotoxicity. Ginsenoside F1 (GF1) is a metabolite formed when the ginsenosides Re and Rg1 are hydrolyzed. However, the protective effects and underlying mechanisms of GF1 on THP-induced cardiotoxicity remain unclear. Methods: We investigated the anti-apoptotic and anti-oxidative stress effects of GF1 on an in vitro model, using H9c2 cells stimulated by THP, plus trigonelline or AKT inhibitor imidazoquinoxaline (IMQ), as well as an in vivo model using THP-induced cardiotoxicity in rats. Using an enzyme-linked immunosorbent test, the levels of malondialdehyde (MDA), brain natriuretic peptide (BNP), creatine kinase (CK-MB), cardiac troponin (c-TnT), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione (GSH) were determined. Nuclear factor (erythroid-derived2)-like 2 (Nrf2) and the expression of Nrf2 target genes, including heme oxygenase-1 (HO-1), glutathione-S-transferase (Gst), glutamate-cysteine ligase modifier subunit (GCLM), and expression levels of AKT/Bcl-2 signaling pathway proteins were detected using Western blot analysis. Results: THP-induced myocardial histopathological damage, electrocardiogram (ECG) abnormalities, and cardiac dysfunction were reduced in vivo by GF1. GF1 also decreased MDA, BNP, CK-MB, c-TnT, and LDH levels in the serum, while raising SOD and GSH levels. GF1 boosted Nrf2 nuclear translocation and Nrf2 target gene expression, including HO-1, Gst, and GCLM. Furthermore, GF1 regulated apoptosis by activating AKT/Bcl-2 signaling pathways. Employing Nrf2 inhibitor trigonelline and AKT inhibitor IMQ revealed that GF1 lacked antioxidant and anti-apoptotic effects. Conclusion: In conclusion, GF1 was found to alleviate THP-induced cardiotoxicity via modulating Nrf2 and AKT/Bcl-2 signaling pathways, ultimately alleviating myocardial oxidative stress and apoptosis.

• 한국컴퓨터정보학회논문지
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• 제28권4호
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• pp.133-142
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• 2023

국립공원은 세계 자연 보존 연맹(WWF)이 수립한 보호지역 관리 체계의 중요 유형 중 하나이며, 또한 자연 및 문화 유산의 효과적인 보호와 지속적인 이용을 실현하는 세계 각국의 관리 모델이다. 이러한 공원은 보호, 과학 연구, 교육, 레크리에이션 및 지역 개발을 비롯한 중요한 역할을 담당하다. 대용량 데이터의 배경 아래, 본 연구는 전 세계 연안 습지의 대표적인 대상인 중국 랴오하 국립공원을 사례 지역으로 삼아 파이썬 기술을 사용하여 중국의 주요 관광 OTA 사이트 중 하나인 망픈웨이 (Mafengwo), 셰어이(Gonglve), 큐난우(Chujingyou), 메이툰(Meituan) 및 대중점평넷(Dianping)의 관광객 여행기와 댓글을 데이터 소스로 수집하였다. 텍스트 시간 범위는 2015년부터 2022년까지이며, 총 2,998개의 댓글과 166,588개의 단어를 포함하다. ROST 콘텐츠 마이닝 및 Gephi 소프트웨어를 사용하여 랴오하 국립공원 방문객의 만족도, 인지 과정, 공선 네트워크, 감정 성향 등을 시각적 분석하였다. 결과는 다음과 같다. 야생 동물 및 식물 자원, 강과 바다가 결합 된 자연 경관, 습지 생태는 랴오하 국립공원 방문객의 인식에서 충분히 반영되었다. 방문객은 랴오하 국립공원에 대해 강한 긍정적인 감정을 가지고 있지만, 시설 서비스, 대중교육, 방문객 참여 경험 등에서 여전히 개선할 여지가 있다.

• 스마트미디어저널
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• 제12권8호
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• pp.9-17
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• 2023

정보통신의 발달과 스마트 기기의 발전 및 보급 향상은 관광 형태의 변화를 야기하였고, 이후 스마트 관광이라는 개념이 등장하였다. 이에 스마트 관광 정책 및 설문에 관한 연구가 진행되고 있으나 애플리케이션 리뷰에 관한 연구는 미비한 편이다. 본 연구는 구글 플레이 스토어 내 스마트 관광 분야의 대표적인 애플리케이션인 트립어드바이저 애플리케이션 리뷰 데이터를 수집하여 LDA(Latent Dirichlet Allocation) 토픽 모델링을 통해 사용 용도와 사용자 만족을 파악하고자 한다. 분석 결과 4개의 토픽이 도출되었으며 2개의 토픽에서는 긍정적인 평가를 나머지 2개의 토픽에서는 부정적인 평가를 하고 있었다. 사용자들은 해당 애플리케이션의 숙박 및 관광 명소 추천 시스템에 만족하고 있음을 알 수 있었으며 검색 시 설정한 필터가 적용되지 않거나 업데이트 후 리뷰가 게시되지 않음에 불편을 겪고 있음을 알 수 있었다. 이에 다양한 추천 카테고리를 애플리케이션에 추가하여 사용자에게 다양한 경험을 제공하는 것이 만족도 향상에 도움이 될 것으로 기대된다. 또한 필터 기능을 포함한 애플리케이션 문제를 파악하여 애플리케이션 환경 점검과 해당 기능 오류 개선을 한다면 사용자 만족도를 향상시킬 수 있을 것으로 기대된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.74-74
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• 2022

본 연구는 붉나무의 기능성 성분의 산업화 활용을 위한 고품질의 붉나무 1년생 용기묘 대량생산을 위해 실시되었다. 붉나무 1년생 용기묘의 묘목품질의 향상을 위해 다양한 농도의 시비처리하여 우량묘 생산에 적합한 시비수준을 구명하고자 하였다. 시비실험은 무시비구 포함하여 수용성 복합비료 (N:P:K=20:20:20, v/v)를 1,000mg·L^-1, 2,000mg·L^-1, 3,000mg·L^-1 수준으로 실시하였다. 시비처리는 붉나무 용기묘의 간장과 근원경 생장은 1,000mg·L^-1 시비처리구에서 생장이 가장 좋게 나타났으며 시비수준이 증가할수록 감소하는 경향을 보였다. 뿌리형태 특성분석에서도 전체뿌리길이, 투영단면적, 표면적 및 뿌리부피가 간장과 근원경 생장과 동일한 경향을 보이는 것으로 조사되었다. 건물 생산량은 부위별 잎, 줄기, 뿌리 및 전체가 모두 1,000mg·L^-1 시비처리구에서 가장 높았으며, 그 이상의 시비수준에서는 점점 감소하는 경향을 보였다. H/D(Hight/Root collar diameter)율은 전체가 3.82(무시비구) ~ 4.90, T/R(Top/Root)율은 전체가 0.56(1,000mg·L^-1 시비처리구) ~ 0.82로 조사되었다. 시비처리 에 의한 붉나무의 LWR(Leaf dry weight ratio)은 무시비구, SWR(Shoot dry weight ratio)은 3,000mg·L^-1 시비처리구, 그리고 RWR(Root dry weight ratio)은 1,000mg·L^-1 시비처리구에서 유의적 차이를 보이며 높게 나타났다. 묘목품질지수(QI)의 경우 1,000mg·L^-1 시비처리구에서 12.13으로 다른 시비수준들보다 월등히 높았다. 본 실험의 결과를 종합하면, 뿌리발달이 좋은 우량한 붉나무 생산에 적절한 시비수준은 1,000mg·L^-1 정도인 것으로 판단된다.

• Animal Bioscience
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• 제36권12호
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• pp.1775-1784
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• 2023

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.