• 한국식품영양과학회지
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• 제33권2호
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• pp.451-454
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• 2004

본 실험의 목적은 Saccharomyces cereuisiea를 단백분해효소로 처리하여 얻은 가수분해물 SCP-20의 첨가가 마우스의 생식 기능에 미치는 영향을 평가하기 위하여 실시하였다. 개시시 체중 32∼33 g의 7주령 ICR 웅성 마우스 40수를 공시하여 SCP-20의 급여가 정소무게 및 정소상체 미부 정자의 운동특성 미치는 영향을 평가하였다. 실험 설계는 분쇄한 가루형태의 일반 마우스 식이를 급여 한 대조군(CON)과 대조군 식이에 효모가수분해물 SCP-20을 1.5% 첨가한 처리군(TRT)으로 하였다. 정소무게에 있어서는 대조군과 비교하여 처리군의 정소무게가 유의적으로 더 높게 평가되었다. (p<0.05). 정자의 직선운동속도(VSL)는 대조군과 비교하여 처리군에서 유의적으로 높았다(p<0.05). 평균경로속도(YAP)와 곡선경로선형도(LIN)는 대조군과 비교하여 처리군이 더 높았다(p<0.05). 또한, 웅성 마우스에게 SCP-20을 급여하면 정소상체 미부 정자의 운동속도가 증가하는 것으로 나타났다(p<0.05). 결론적으로, 마우스에게 SCP-20를 급여하면 정자의 운동속도가 증가하는 것으로 나타났다.

• BMB Reports
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• 제55권2호
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• pp.98-103
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• 2022

Increased mRNA levels of cancer upregulated gene (CUG)2 have been detected in many different tumor tissues using Affymetrix microarray. Oncogenic capability of the CUG2 gene has been further reported. However, the mechanism by which CUG2 overexpression promotes cancer stem cell (CSC)-like phenotypes remains unknown. With recent studies showing that pyruvate kinase muscle 2 (PKM2) is overexpressed in clinical tissues from gastric, lung, and cervical cancer patients, we hypothesized that PKM2 might play an important role in CSC-like phenotypes caused by CUG2 overexpression. The present study revealed that PKM2 protein levels and translocation of PKM2 into the nucleus were enhanced in CUG2-overexpressing lung carcinoma A549 and immortalized bronchial BEAS-2B cells than in control cells. Expression levels of c-Myc, CyclinD1, and PKM2 were increased in CUG2-overexpressing cells than in control cells. Furthermore, EGFR and ERK inhibitors as well as suppression of Yap1 and NEK2 expression reduced PKM2 protein levels. Interestingly, knockdown of β-catenin expression failed to reduce PKM2 protein levels. Furthermore, reduction of PKM2 expression with its siRNA hindered CSC-like phenotypes such as faster wound healing, aggressive transwell migration, and increased size/number of sphere formation. The introduction of mutant S37A PKM2-green fluorescence protein (GFP) into cells without ability to move to the nucleus did not confer CSC-like phenotypes, whereas forced expression of wild-type PKM2 promoted such phenotypes. Overall, CUG2-induced increase in the expression of nuclear PKM2 contributes to CSC-like phenotypes by upregulating c-Myc and CyclinD1 as a co-activator.

• Archives of Plastic Surgery
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• 제49권2호
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• pp.195-199
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• 2022

Nasolacrimal duct (NLD) damage is associated in the majority of type II and III naso-orbito-ethmoid (NOE) fractures. Our study aims to investigate the efficacy and safety of prophylactic NLD intubation in the setting of facial fractures, by comparing incidence of postoperative epiphora and wound infection. A retrospective matched control study was conducted on all patients with surgically treated facial fractures from 2008 to 2013 (n=280) (IRB ref number: DSRB 2013/01198). Patients with the following fracture types were included: NOE (n=16), frontal sinus (n=2), Le Fort II/III (n=8), and > 1 type (n=48). All patients in this study were included with the intention to treat. The study group comprised patients who were intubated, while the control group patients were not intubated. Each group had 37 patients matched for age, gender, fracture type, and injury type. A single oculoplastic surgeon skilled in lacrimal surgery performed the procedure for all intubated patients. Patients with more severe and complex facial fractures were intubated with bicanalicular Crawford stents. Postoperative epiphora and infective complications (both facial wound and dacryocystitis) were assessed at 1, 3, 6, and 12 months. There was no significant difference in incidence of either postoperative epiphora (p=0.152) or wound infection (p=0.556) comparing both groups. Reduced incidence of postoperative epiphora in the study group is statistically not significant and does not support the need for prophylactic intubation. If radiographic evidence of NLD disruption or regurgitation seen on syringing on the NLD intraoperatively is present, intubation is safe and efficacious only if performed by an expert.

• Archives of Plastic Surgery
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• 제41권6호
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• pp.638-646
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• 2014

Background The combination of polycaprolactone and hyaluronic acid creates an ideal environment for wound healing. Hyaluronic acid maintains a moist wound environment and accelerates the in-growth of granulation tissue. Polycaprolactone has excellent mechanical strength, limits inflammation and is biocompatible. This study evaluates the safety and efficacy of bio-conjugated polycaprolactone membranes (BPM) as a wound dressing. Methods 16 New Zealand white rabbits were sedated and local anaesthesia was administered. Two $3.0{\times}3.0cm$ full-thickness wounds were created on the dorsum of each rabbit, between the lowest rib and the pelvic bone. The wounds were dressed with either BPM (n=12) or Mepitel (n=12) (control), a polyamide-silicon wound dressing. These were evaluated macroscopically on the 7th, 14th, 21st, and 28th postoperative days for granulation, re-epithelialization, infection, and wound size, and histologically for epidermal and dermal regeneration. Results Both groups showed a comparable extent of granulation and re-epithelialization. No signs of infection were observed. There was no significant difference (P>0.05) in wound size between the two groups. BPM (n=6): $8.33cm^2$, $4.90cm^2$, $3.12cm^2$, $1.84cm^2$; Mepitel (n=6): $10.29cm^2$, $5.53cm^2$, $3.63cm^2$, $2.02cm^2$; at the 7th, 14th, 21st, and 28th postoperative days. The extents of epidermal and dermal regeneration were comparable between the two groups. Conclusions BPM is comparable to Mepitel as a safe and efficacious wound dressing.

• 대한약침학회지
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• 제7권3호
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• pp.5-25
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• 2004

Objectives : To observe changes in the serum proteins before and after intravenous injection of wild ginseng herbal acupuncture. Methods : Blood was collected before and after the administration of wild ginseng herbal acupuncture and only the serum was centrifuged. Then differences in the spots on the scanned image after running 2-Dimensionl electrophoresis were located and conducted mass analysis and protein identification. Results : Following results were obtained from the comparative analysis of serum proteins before and after the administration of wild ginseng herbal acupuncture. 1. 28 spots were identified before and after the administration. 2. In confirming manifestation degree, spots with more than two-times increase were 204, 803, 1505, 2205, 3105, 7104, 9001 spots, with more than one-time increase were 1101, 1302, 2013, 3009, 3010, 4002, 4009, 6706, 7103, 8006, 8101, and spots with decrease were 205, 801, 3205, 5202, 6105. 3. After conducting protein identification, proteins 205, 804, 1302, 4009, 6105, 6106 are unidentified yet, and 1101 is unnamed protein. Protein 204 is identified as complement receptor CR2-C3d, 801 as YAP1 protein, 803 as antitrypsin polymer, 1505 as PRO0684, 2013 and 3010 as proapolipoprotein, 2205 as USP48, 2403 as vitamin D binding protein, 3009 as complement component 4A preprotein, 3105 as immunoglobulin lambda chain, 3205 as transthyretin, 4002 as Ras-related protein Ral-A, 4204 as beta actin, 5202 and 7104 as apolipoprotein L1, 6704 as alpha 2 macroglobulin precursor, 7103 as complement component 3 precursor, 8006 as testis-specific protein Y, 8101 as Transferrin, 9001 as(Alpha-Oxy, Beta-(C112g)deoxy) T-State Human Hemoglobin, and 9003 as human hemoglobin. 4. Immune protein CR2-C3d, which acts against microbes and pathogenic organisms, and Antitrypsin(803), which is secreted with inflammatory response in the lungs, were increased by more than 200% after the administration of herbal acupuncture. 5. Immunoglobulin lambda chain(3105), Alpha-Oxy, Beta-(C112g)deoxy T-State Human Hemoglobin(9001), and human hemoglobin(9003) were increased by more than two-times after the administration of herbal acupuncture. 6. Proapolipoprotein(2013, 3010) and apolipoprotein(7104), key components of the HDL-cholesterol which plays an important role in preventing arteriosclerosis, were increased after the administration of herbal acupuncture. 7. Vitamin D binding protein(DBP, 2403), protecting the lung at the time of inflammatory response, was increased after the administration of herbal acupuncture. 8. Transthyretin(TTR, 3205), which is the main protein causing familial aimyloid polyneuropathy(FAP), was decreased after the administration of herbal acupuncture. 9. Ras-related protein Ral-A(4002) that controls phospholipid metabolism, cytoskeletal formation, and membrane traffic, was increased after the administration of herbal acupuncture. 10. Testis-specific protein Y(8006), which takes part in determination of the gender, was increased by more than two-times after the administration of herbal acupuncture. 11. Transferrin(8101), T-State Human Hemoblobin(9001), and Human Hemoblobin(9003) which balances the iron level in the body, were increased after the administration of herbal acupuncture. Conousion : Above results support the notion that intravenous injection of cultivated wild ginseng herbal acupuncture induce changes in serum proteins and this research can be a pioneer work in finding biomarkers.

• 한국지구과학회지
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• 제26권5호
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• pp.395-403
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• 2005

본 연구는 1993년부터 2003년 동안 겨울철 제주도 지방에 눈이 내린 경우를 대상으로 대기가 해양으로부터 얻은 열교환량을 계산하여 보웬비(현열속/잠열속)와 강설간의 관계를 분석한 것이다. 대상지역은 제주도 4개 관측지점인 제주, 서귀포, 성산포, 고산이다. 적설 예측의 신뢰도를 높일 수 있는 방법을 찾기 위하여 바람과 같은 기상의 영향이 가미된 보웬비와 적설과의 관계를 해기차와 적설과의 관계와 비교해 보았다. 그 결과 신적설 시, 지역별 최저 해기차는 제주시, 서귀포, 성산포, 고산에서 각각 10.9, 12.3, 11.5, $14.3^{\circ}C$였고, 그 이상의 해기차에서 신적설 확률은 각각 26, 29, 13, $23\%$인데 비해, 신적설시 지역별 최저 보웬비는 각각 0.59, 0.60, 0.65, 0.65였고, 그 이상의 보웬비에서 신적설 확률은 33, 70, 31, $58\%$로 나타나 보웬비가 해기차보다 높은 확률을 보였다. 보웬비에 의한 확률이 해기차에 의한 확률보다 높게 나타나는 이유는 해기차에 의해 형성된 강설의 조건에 바람에 의한 열교환이 강설의 조건을 강화시킨 것이 보웬비에 나타났기 때문으로 생각된다. 10년$(’93\~’02)$간 자료를 분석한 결과 각 지역별 신적설이 $10.0\~0.9cm$ 일 때 평균 보웬비는 $0.63\~0.67$이고 신적설이 $1.0\~4.9cm$ 일때 평균 보웬비는 0.72 이상으로 조사되어 강설시 적설량과 보웬비는 비례관계가 있음을 알 수 있었다.