• Journal of the Korean Chemical Society
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• v.44 no.1
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• pp.17-23
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• 2000

Electrodes of the polycationic film with electropolymerized $[Ru(v-bpy)_3]^{2+}$ having about 1:1 ratio of $PF6^-/ClO_4^-$as the doped counter ions, were modified with xylenol orange and diethylditbiocarbamate by ion exchange which had stability constant as 38.6 and 17.5 respectively. These electrodes were employed in the quantitative multiple determination of U(W) in solution. The working electrode of electrochemical cell for the analytical signal was Pt/p-$[Ru(v-bpy)_3]^{2+}$, ligand, U(VI) with Ag/AgCl reference elecrode. In the stripping voltammetry. electrode process was electron transfer controlled one and calibration curves at the ranges of $1.0{\times}10^{-3}{\sim}1.0{\times}10^{-7}$ M had excellent relationship as 0.99 and relative standard deviation as 5${\sim}$8%.

• KSBB Journal
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• v.17 no.2
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• pp.195-202
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• 2002

The production conditions of cellulolytic enzymes by a fungus, strain FJ1, were optimized using response surface analysis. The culture factors which largely affected the production of enzymes such as cultivation time, carbon source concentration, nitrogen source concentration, and composition ratio of carbon sources were employed. Optimizedconditions of the factors above corresponding to each cellulolytic enzyme production were as fellowing: CMCase production was obtained in the conditions of cultivation time of 5.4 days, carbon source concentration of 3.5%, nitrogen source concentration of 0.6%, and composition ratio of carbon sources of 52:48 (avicel:CMC), xylanase appeared in the conditions of 5.3 days, 3.5%, 0.8%, and 54:46, respectively, and $\beta$-glucosidase were 7.0 days, 5.0%, 1.0%, and 83:17, respectively, and avicelase were 6.5 days, 4.0%, 0.9%, and 64:36, respectively. The activities of CMCase, xylanase, p-glucosidase, and avicelase predicted by the response surface methodology were 33.5, 52.6, 2.88, and 1.84 U/mL, respectively, and $\beta$-glucosidase activity was enhanced up to 74% when compared to that obtained in the experimental conditions.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.453-456
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• 2002

The production conditions of cellulolytic enzymes by Trichoderma sp. FJ1, were optimized using response surface analysis. The culture factors which largely affected to the production of enzymes such as cultivation time, carbon source concentration, nitrogen source concentration, and composition ratio of carbon sources were employed. Optimized conditions of the factors above to each cellulolytic enzyme production was as follow: CMCase production was obtained in the conditions of cultivation time of 5.4 days, 3.5% of carbon source concentration, 0.6% of nitrogen source concentration, and 52:48 (avicel:CMC) of composition ratio of carbon sources, respectively, xylanase appeared in the conditions of 5.3 day, 3.5%, 0.8%, and 54:46, respectively, and ${\beta}-glucosidase$ were 7.0 day, 5.0%, 1.0%, and 83:17, respectively, and avicelase were 6.5 day, 4.0%, 0.9%, and 64:36, respectively. The activities of CMCase, xylanase, ${\beta}-glucosidase$, and avicelase predicted by the response surface methodology were 33.5, 52.6, 2.88, and 1.84 U/ml, respectively, and ${\beta}-glucosidase$ was enhanced up to 74% compared to that obtained in the experimental conditions.

• Journal of Mushroom
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• v.10 no.2
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• pp.74-82
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• 2012

Sixty strains of Pleurotus ostreatus, white-rot fungi, were screened for production ability of their lignocellulolytic enzymes to selectively wood degradation. That results were shown that all of screened strains were produced lignocellulolytic enzymes on 2nd screening liquid culture medium. However, cellulase activity of selected six strains of P. ostreatus was low in avicel-yeast-peptone liquid culture medium. In the case of xylan degrading enzyme, No. 6 and No. 38 strains produced a xylanase(above 1.0U/ml) and a 1,4-${\beta}$-xylosidase (above 0.15 U/ml). Examination of the ligninolytic enzyme profiles of selected thirteen strains of the P. ostreatus, in the presence of Remazol Brilliant Blue R(RBBR), were observed that laccase(Lac) activity were earlier reached maximum level(0.8-2.0 U/ml) and then Mn-dependent peroxidase (MnP) were reached maximum level(0.5-1.5 U/ml) in glucose-yeast-peptone(GYP) medium. On the other hand, activity of lignin peroxidase(LiP) was not detected in this medium. I selected the No. 42 strain of P. ostreatus produced high levels of Mn-dependent peroxidase and laccase based on the screening method.

• KSBB Journal
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• v.17 no.4
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• pp.381-387
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• 2002

A filamentous fungus, strain FB01 showing high $\beta$-glucosidase activity was isolated from a compost. This fungus was cocultured with Trichoderma viride to enhance the productivity of $\beta$-glucosidase by changing inoculation time of the fungus. The microbial consortium showed higher cellulolytic enzyme production than T. viride alone. The maximal enzyme production was obtained when the microbial consortium was cultured at 30$\^{C}$ and pH 6.0 for 10 days with the activities of CMCase, $\beta$-glucosidase, and avicelase of 2.0, 0.8, and 0.2 U/mL, respectively. These enzyme activities were 2, 4, and 2 times as high as those of CMCase, p-glucosidase, avicelase from T. viride, respectively, indicating that a synergistic interaction appeared between T. viride and strain FBOI . The serial subcultures with pH control increased $\beta$-glucosidase production about 3.2 times. Enzyme production using ricestraw as a carbon source showed that the activities of CMCase, $\beta$-glucosidase, and avicelase were 3.69, 0.76, 0.17 U/mL, respectively, and $\beta$-glucosidase activity was 1.5 times higher than that of T viride.

• Journal of Aquaculture
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• v.16 no.1
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• pp.24-30
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• 2003

Studies of the seed production of Urechis unicinctus were conducted under the laboratory conditions to obtain some information for the U.unicinitus culture. The experiment included developmental studies of the egg development, larval culture, sediment preference and growth of young U.unicinctus. The experiment were conducted from March to August, 2000. The adults of U.unicinctus collected in Namhae-do, Korea. The developments of the fertilized eggs were observed under a light-microscope at intervals of one hour after containing with density of one individual per 1 $m\ell$. The larvae were fed with Phaeodactylum tricornutum cultured at the laboratory. The concentration of the phytoplankton for the feed was 30,000 cells per individual larva. With progress of development, the food concentration was gradually increased, up to 10,000 cells per individual for the young U.unicinctus. Trochophore larvae appeared on the 68 hours after hatching. On the 32 days after hatching, over 50% of fertilized eggs developed into young U.unicinctus. In order to investigate the effect of sediment on the growth and burrowing of U.unicinctus, the young worms were reared in tanks with different grain sizes. The highest value of sediment preference and survival rate of U.unicinctus was shown in the mixture sediment group with below 0.10 mm, 1.01∼12.00 mm, over 3.01 mm and shell. The lowest value in both sediment preference and survival rate of U.unicinctus was observed in 1.0l∼2.00 mm grain size.

• Journal of Aquaculture
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• v.6 no.2
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• pp.71-87
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• 1993

Two forms of sea mustard, Undaria Pinnatifida were cultured in order to compare the morphological characteristics and the efficiency of production. U. Pinnatifida f. distans was collected from Wando, Korea and U. Pinnatifida f. typica was transplanted from Sanriku, Japan. The latter form grew faster in length and weight than the former. Maximum growth was shown in early April for both forms, and the growth rates decreased after this. Absolute growth rates between the two forms were quite different. Average total lengths at the harvest season was 161.1cm in U. Pinnatifida f. distans and 183.5 em in U. Pinnatifida f. typica and average weights of those two forms were 1,003.4g and 1,314.6g, respectively. The weight of available parts for the salting process of the two forms were 734.8g and 968.5g, respectively. The rates of total length versus the length of the available part of the two forms were $76\%$ and $83\%$, respectively and those of the total length versus weight of the available part of them were $43.8\%$ and $52.7\%$, respectively. The results of this study suggest that the U. pinnatifida f. typica had more available part per unit weight than that of the U. pinnatifida f. distans for the efficiency of salt processing.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.3
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• pp.240-246
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• 2019

The aim of this study was to investigate effect of heat stress on expression levels of plasminogen activators (PAs) related mRNAs and proteins, and changes of PAs activity in porcine endometrial explants. The endometrial explants (200 ± 50 mg) were isolated from middle part of uterine horn at follicular phase (Day 19-21) and were pre-incubated in serum-free culture medium at 38.5℃ in 5% CO₂ for 18 h. Then, the tissues were transferred into fresh medium and were cultured at different temperature (38.5, 39.5, 40.5 or 41.5℃) for 24 h. The expression level of urokinase-type PA (uPA), type-1 PA inhibitor (PAI-1), type-2 PAI (PAI-2), and heat shock protein-90 (HSP-90) mRNA were analysis by reverse-transcription PCR and proteins were measured by western blotting. The supernatant were used for measurement of PAs activity. In results, mRNA and protein levels of HSP-90 was higher in 41.5℃ treatment groups than other treatment groups (p < 0.05). The expression of uPA, PAI-1, and PAI-2 mRNA were slightly increased by heat stress, however, there were no significant difference. Heat stress condition suppressed expression of active uPA and PAI-2 proteins (p < 0.05), whereas PAI-1 protein was increased (p < 0.01). Although PAI-1 protein was increased and active uPA was decreased, PAs activity was greatly enhanced by exposure of heat stress (p < 0.05). These results suggest that heat stress condition could change intrauterine microenvironment through regulation of PAs activity and other factors regarding with activation of PAs might be regulate by heat stress. Therefore, more studies regarding with regulatory mechanism of PAs activation are needed.

• Journal of the Korean Wood Science and Technology
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• v.35 no.4
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• pp.61-66
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• 2007

The study was performed to purify and characterize laccase in culture of Trametes versicolor. The fungus was grown in liquid culture media of PDB and added 2,5-xylidine (0.2 mM) after 5 days to enhance the production of laccase. The fungal culture was incubated at $25^{\circ}C$ on a rotary shaker (120 rpm) for 7days, and the culture broth was clarified through Glass filter (GF/C). The aqueous solution was concentrated by ultramicrofiltration (Viva flow 50, GE Healthcare Bioscience, USA) and loaded onto a Hitrap Q FF column. Laccase activity could be detected at one peak, and this enzyme has a molecular mass of approximately 53kDa as determined by SDS-PAGE The optimum pH and temperature for syringaldazine were 5.0 and $60^{\circ}C$, respectively. The specific activity of crude, concentrated and purified laccase were 32, 409, and 1,243 U/mg, respectively.

• LP가스
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• s.78
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• pp.4-5
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• 2002