• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.172-176
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• 2004

본 연구는 축우의 모색발현을 조절하는 MCIR, MGF 및 TYRP1 3종류의 모색 유전자를 이용하여 한우육 판별기술을 개발하고자 PCR-RFLP 기법으로 이들 모색유전자 좌위의 대립유전자를 검출하고 각 품종 간 RFLP 유전자형 출현빈도를 비교 분석하였다. MCIR 유전자의 RFLP 유전자형 출현빈도에서 한우는 e/e과 E+/e형이 출현되었고 이외의 다른 유전자형의 출현은 전혀 인정되지 않았다. 그러나, Holstein종 젖소는 $E^D/E^D$와 $E^D/e$ 2종류의 유전자형 그리고 Angus종에서는 $E^D/E^D$, $E^D/E^++$ 및 $E^D/e$ 3종류의 유전자형이 각각 출현하여 한우와 이들 두 품종간의 MCIR유전자형 출현빈도에 뚜렷한 차이가 인정되었다. MGF 유전자의 RFLP 유전자형 출현빈도에서 한우는 R/r과 r/r형이 각각 25%와 75%로 rr형의 출현율이 비교적 높았으며 Holstein종과 Angus 종은 R/r형이 100% 출현했으며, Charolais 종은 rr형이 100% 출현하였고 이외의 다른 유전자형은 인정되지 않았으며 Hereford종은 RR형이 80% 그리고 R/r형이 20%의 출현율을 보여 RR형의 출현율이 매우 높아 한우와 Holstein 및 육우 품종간의 MGF 유전자형 출현빈도에 명백한 차이가 인정되었다. 따라서, 소 모색관련 MCIR과 MGF 유전자의 품종 특이적 PCR-RFLP 유전자형은 한우육과 국내산 Hostein 젖소육 및 도입육우 품종을 식별하는데 매우 유용한 DNA marker로 이용될 수 있음이 확인되었다.

• Polymer(Korea)
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• v.25 no.5
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• pp.625-634
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• 2001

New polymeric photostabilizers containing hindered amine light stabilizer (HALS) were prepared by the reaction of liquid polyisoprene rubber grafted maleic anhydride (MAH) and 2,2,5,6-tetramethyl-4-piperidinol (TMPO). Their chemical composition and physical properties were characterized by titration, GPC and TGA analysis. The effects of polymeric HALS on the photooxidation of the styrene-butadiene rubber were studied from the UV, IR spectral changes, and photo-crosslinking was examined by the measurement of the insoluble fraction. The photooxidation of SBR upon irradiation was inhibited by addition of the new polymeric HALS. The extraction resistance of new polymeric photostabilizer was much better than that of the low molecular weight compound which is prepared by the reaction of MAH and TMPO. The new polymeric HALS ate fairly compatible with the SBR.

• Korean Journal of Poultry Science
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• v.37 no.1
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• pp.89-99
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• 2010

Newcastle disease (ND), caused by Newcastle disease virus (NDV), has caused periodic epidemics in Korea at an interval of 3 to 5 years until the early 2000s. At least five distinct genotypes of NDV have been responsible for epizootic episodes in Korea; genotype III virus (before the 1970s), genotype V (the mid-1980s), genotype VI (the late 1980s to early 1990s), genotype VIIa (the mid-1990s), and genotype VIId viruses (the late 1990s to present). Recent epidemic strains of NDV (VIId viruses) shared geographical features with neighboring countries such as China and Japan. These VIId viruses as well as genotypes III and V viruses were viscerotropic and highly virulent for chickens. Antigenic variation occurred between VIId field viruses and LaSota vaccine strain, as found in other epidemic strains in past in Korea. Nevertheless the commercial vaccine was considered to effectively protect vaccinated birds from mortality against VIId viruses as well as other viruses belonging to genotypes III and V.

• 베이커리
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• no.7 s.372
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• pp.74-75
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• 1999

• Journal of the Korean Society of Propulsion Engineers
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• v.2 no.2
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• pp.64-73
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• 1998

In twin spool aero-engine, there may be a S shaped annular duct between high pressure and low pressure spools. The flow passing this S shaped duct experiences the flow acceleration and deceleration due to the convex and concave surface of the duct as well as the increase of blockage according to the boundary layer growth along the surfaces. So, the high pressure compressor which is located behind the S shaped duct is influenced by the non-uniform flow field generated by the geometry of inlet duct. To study the influence of the S shaped duct on the centrifugal stage, performance tests were implemented for the compressor with straight cylindrical inlet duct and with S shaped inlet duct, respectively. The test results showed that the performance, such as pressure ratio and efficiency, of the compressor with S shaped duct was worse than that of the compressor with cylindrical duct. And the compressor with S shaped duct had reduced maximum flow rate around design speed. To investigate the cause of performance degradation, flow anlaysis was performed for the impeller in front of which is located S shaped annular duct. The result of CFD showed the strong acceleration of the flow in the axial direction around the inducer tip region which caused the increase of relative mach number and the decrease of incidence angle of the flow.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.12 no.2
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• pp.179-191
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• 2001

Objectives：This study evaluated the association between behavioral characteristics and polymorphisms in DAT1, DRD2, DRD3, and DRD4 genes. Methods：The subjects were 114 neonates, who were born by normal spontaneous vaginal delivery and had no physical problems. The behavioral characteristics were evaluated using Neonatal Behavioral Assessment Scale(NBAS) at $17.8{\pm}7.0$ hours after their birth to minimize environmental influences, and cord blood was used to analyze the gene polymorphisms. Results：In comparison to DAT1 gene 10/10 genotype group(N=93), other genotype group(N=19) showed significantly high NBAS scores on social-interaction, state organization, and state regulation. DRD2 gene Ser311/Cys311, TaqI A, and TaqI B polymorphisms showed no significant differences on NBAS scores when they were grouped by genotypes. DRD3 gene polymorphism and DRD4 gene promotor polymorphism showed no significant difference on NBAS scores when they were grouped by genotypes. In comparison to the short repeats(N=102), long repeats(N=10) in DRD4 gene showed significantly high habituation score of NBAS. Conclusion：These results suggest that the genetic effects of the neonatal behavioral characteristics may be mediated via DAT1 and DRD4 genes.

• Journal of Life Science
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• v.24 no.8
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• pp.910-914
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• 2014

For the identification of the Jeju black cattle, Hanwoo and imported beef, we performed a multiplex polymerase chain reaction (PCR) associated with microsatellite (MS) and melanocortin 1 receptor (MC1R) gene. The MC1R gene plays an important role in regulation of the melanin synthesis within mammalian melanocytes. MC1R encoded by extension (E) locus was almost fixed with recessive red e allele in the Hanwoo. We estimated that the specific genotypes ($E^+/E^+$, $E^+/e$) of MC1R gene were characteristic genotypes of Jeju black cattle. But the PCR products resulted from using the MC1R gene derived primers only are not sufficient to identify Jeju black cattle from other relatives. We performed two times of successive multiplex PCR to provide a more accurate result for the identification of Jeju black cattle. The results suggest that two types of successive multiplex PCR methods using MC1R gene and Microsatellite derived primer set will be more useful to identification of Jeju black cattle, Hanwoo and imported beef.

• Journal of Life Science
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• v.23 no.2
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• pp.175-181
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• 2013

In Korea, most hepatitis A virus is the IA genotype, but reports of other genotypes have increased recently. Therefore, the purpose of this study is to conduct a genotypic analysis of acute hepatitis A virus. From April 2010 to April 2011, clinical specimens from 20 patients hospitalized with acute hepatitis A and 36 sera positive for anti-HAV IgM were obtained, and the genotype of the VP1/P2A region was analyzed. RNA sequences of the VP1/P2A junction region were amplified using RT-PCR, and the sequences were compared. From 50 sequences amplified, 4 sequences (8%) belonged to genotype IA. The remaining 46 (92%) belonged to genotype IIIA. The results indicate that the genotype of the hepatitis A virus has changed from IA to IIIA in Korea.

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.151-156
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• 2007

The Function of the calcium sensing receptor (CaSR) is to control calcium levels by altering PTH (parathyroid hormone) secretion and renal calcium resorption. The influence of calcium on the basal and stimulated release of several hormones from chicken pituitary glands has been determined in vitro. The objective of this study was to identify SNP in chicken CaSR gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms within chicken CaSR gene. This study identified SNP at position 1949 bp(Genebank accession No : XM_416491) in the exon 1. The SNP changed the amino acid to alanine(GCC) from serine(TCC). This SNP showed three genotypes, AA, AS and SS by digestion with the restriction enzyme NcoⅠ using the PCR-RFLP method. The A963S showed significant effect only on the first lay day (P<0.05) in Leghorn population. Leghorn with the genotype AA had significantly faster the first lay day(137.6) than the genotype AS(143.0, P<0.05). Also, the A963S showed significant effect only on the first lay day(P<0.05) and mean of egg weight(P<0.05) in KNC population. KNC with the genotypes AA ans AS had significantly faster the first lay day (151.0 and 152.6, respectively) than the genotype SS(159.4, P<0.05). And the genotypes SS had significantly heavier the mean of egg weight(50.4 kg, P<0.05) than the genotype AA ans AS (47.5 and 47.8 kg, respectively). According to result of this study, an a allele of the A963S was found to have a significant effect on the first lay day. It will be possible to use this SNP marker on selecting chicken to improve the first lay day.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.458-464
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• 1994

The partitioning of dry matter into seed and pod-shell is important because yield increase can be achieved by improving the distribution rate of assimilation products to seeds. The present study was undertaken to characterize whether the partitioning of dry matter into seed was shoot- or root-controlled through graftiong techniques. Self- and reciprocal-grafts were made among four soybean genotypes, which were 'Baekunkong', 'Suwon 168', and two local soybeans with black seed coat (hereafter referred to as the 'black soybean'), 'Kangleungjarae' and 'Keumleungjarae'. Self-grafted black soybeans showed lower ratio of seed to pod-shell dry wight than self-grafted Baekunkong and Suwon 168. Varying the shoot genotypes in grafts resulted in significant differences in growth characters as well as pod and seed dry weight per plant at physiological maturity. There were significant effects of shoot genotypes on the ratio of seed to pod-shell dry weight, indication that the partitioning of dry matter into seed and pod-shell should be shoot- rather than root-controlled. The grafts with large pod-shell dry weight tended to partition relatively smaller dry matter into seed than pod-shell.