• Journal of the Chungcheong Mathematical Society
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• v.24 no.2
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• pp.287-301
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• 2011

Let X and Y be vector spaces. It is shown that a mapping $f\;:\;X{\rightarrow}Y$ satisfies the functional equation $$(\ddag)\hspace{50}dk\;f\left(\frac{\sum_{j=1}^{dk}x_j}{dk}\right)=\displaystyle\sum_{j=1}^{dk}f(x_j)$$ if and only if the mapping $f$ : X ${\rightarrow}$ Y is Cauchy additive, and prove the Cauchy-Rassias stability of the functional equation ($\ddag$) in Banach modules over a unital $C^{\ast}$-algebra. Let $\mathcal{A}$ and $\mathcal{B}$ be unital $C^{\ast}$-algebras. As an application, we show that every almost homomorphism $h\;:\;\mathcal{A}{\rightarrow}\mathcal{B}$ of $\mathcal{A}$ into $\mathcal{B}$ is a homomorphism when $h((k-1)^nuy)=h((k-1)^nu)h(y)$ for all unitaries $u{\in}\mathcal{A}$, all $y{\in}\mathcal{A}$, and $n$ = 0,1,2,$\cdots$. Moreover, we prove the Cauchy-Rassias stability of homomorphisms in $C^{\ast}$-algebras.

• Journal of radiological science and technology
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• v.44 no.5
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• pp.465-471
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• 2021

Dual-energy X-ray imaging (DEI) techniques can provide X-ray images that a certain material is suppressed or emphasized by combining two X-ray images obtained from two different x-ray spectrum. In this paper, a single-shot DEI, which uses stacked two detectors (i.e., multilayer detector), is proposed to reduce the patient dose and increase throughput in angiography. The polymethyl methacrylate (PMMA) and aluminum (Al) were selected as two basis materials for material decomposition, and material-specific images are reconstructed as a vector combination of these two materials. We investigate the contrast and noise performance of material-decomposed images using iodine phantoms with various concentrations and diameters. The single-shot DEI shows comparable performances to the conventional dual-shot DEI. In particular, the single-shot DEI shows edge enhancement in material-decomposed images due to the different spatial-resolution characteristics of upper and lower detectors. This study could be useful for designing the multilayer detector including scintillators and energy-separation filter for angiography purposes.

• Journal of KIISE:Software and Applications
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• v.30 no.5_6
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• pp.466-475
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• 2003

To build an efficient Question-Answering (QA) system, a question type classifier is needed. It can classify user's queries into predefined categories regardless of the surface form of a question. In this paper, we propose a question type classifier using a Support Vector Machine (SVM). The question type classifier first extracts features like lexical forms, part of speech and semantic markers from a user's question. The system uses $X^2$ statistic to select important features. Selected features are represented as a vector. Finally, a SVM categorizes questions into predefined categories according to the extracted features. In the experiment, the proposed system accomplished 86.4% accuracy The system precisely classifies question type without using any rules like lexico-syntactic patterns. Therefore, the system is robust and easily portable to other domains.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.49-49
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• 2003

본 연구에서는 vesicular stomatitis virus G glycoprotein (VSV-G)를 envelope로 가지는 pantropic retrovirus vector system을 이용하여 재조합 human FSH 유전자가 전이된 형질전환 닭을 생산하고자 하였다. Human FSH $\alpha$ 및 $\beta$ 유전자와 CTP linker는 human pituitary gland cDNA library에서 RT-PCR 방법을 이용하여 cloning하였으며, 각각의 fragment는 FSH$\beta$-CTP-FSH$\alpha$ 순서의 단일사슬로 연결하였다. 연결된 FSH$\beta$-CTP-FSH$\alpha$는 retroviral vector 내의 $\beta$-actin promoter의 조절 하에 도입한 후, PT67 packaging cell line에 transfection하여 virus를 생산하였으며 생산된 virus는 pantropic한 virus producing cell인 GP293에 infection하여 FSH 유전자가 도입된 virus를 생산하였다. FSH 유전자의 발현을 in vitro에서 확인하기 위하여 CHO (chinese hamster ovary) 세포에 virus를 감염시킨 후, 세포의 배양액을 취하여 electrochemilumine-scence immunoassay 방법으로 정량하였다. In vitro에서 전이 후 발현이 확인된 FSH 외래유전자의 retroviral vector virus를 초원심분리로 고농축하여 stageX의 계란의 배반엽 층에 주입하였으며, 그 결과 18%의 부화율과 91%의 부화한 닭의 유전자 전이율을 확인할 수 있었다. 전이된 유전자의 확인은 FSH$\beta$와 Neo 유전자에 대한 primer를 이용한 RT-PCR의 방법을 이용하였다. In vitro에서와는 달리 in vivo에서는 FSH 유전자의 전이는 확인되었으나 발현을 확인하지는 못하였는데, 이는 적은 수의 실험군이 형질전환율에 비해 상대적이지 못하였거나, 외래 유전자인 FSH의 발현에 의한 생리적인 부작용이 유발되어 해당개체가 부화되지 못한 것으로 추정된다. 본 문제점을 해결하기 위하여 실험군의 수를 늘리고 외래 유전자에 대한 controllable expression system이 보완될 필요성이 요구되며, 이러한 점이 해결된다면 높은 유전자 전이율에 기인하여 retrovirus를 이용한 형질전환 방법은 형질전환 가금의 생산에 있어서 매우 효율적이고 주목할 만한 방법으로 사료된다.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1799-1808
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• 2006

A food-grade integration vector based on site-specific recombination was constructed. The 5.7-kb vector, pIMA20, contained an integrase gene and a phage attachment site originating from bacteriophage A2, with the ${\alpha}$-galactosidase gene from Lactobacillus plantarum KCTC 3104 as a selection marker. pIMA20 was also equipped with a controllable promoter of nisA ($P_{nisA}$) and a signal peptide-encoding sequence of usp45 ($SP_{usp45}$) for the production and secretion of foreign proteins. pIMA20 and its derivatives mediated site-specific integration into the attB-like site on the Lactococcus lactis NZ9800 chromosome. The vector-integrated recombinant lactococci were easily detected by the appearance of blue colonies on a medium containing $X-{\alpha}-gal$ and also by their ability to grow on a medium containing melibiose as the sole carbon source. Recombinant lactococci maintained these traits in the absence of selection pressure during 100 generations. The ${\alpha}-amylase$ gene from Bacillus licheniformis, lacking a signal peptide-encoding. sequence, was inserted downstream of $P_{nisA}\;and\;SP_{usp45}$ in pIMA20, and the plasmid was integrated into the L. lactis chromosome. ${\alpha}-Amylase$ was successfully produced and secreted by the recombinant L. lactis, controlled by the addition and concentration of nisin.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.9
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• pp.4152-4158
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• 2012

The Stereo X-ray inspection system is designed for effectively providing the additional information of objects than the conventional inspection system that offers only 2D cross-section of objects. We studied the geometric improvement of the stereo X-ray inspection system, the stereo matching algorithm of the single object using the edge and the volume reconstruction method for the inspected object. In this paper, we conduct a matching algorithm to find the correspondences between the images and reconstruct 3-D shapes of real objects using the stereo X-ray images. Also, we apply a new 3D reconstruction algorithm for the discrimination of two objects. For the separation of the overlapping objects, we calculate the vector of the object and divide inner and outer voxel of objects. And for the elimination of the overlapping area, we study the reconstruct 3D shapes using the threshold based Z-axis. The experimental results show that the proposed technique can enhance the accuracy of stereo matching and give more efficient visualization for overlap objects in the restricted environment.

• The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
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• v.3 no.1
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• pp.20-27
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• 1992

A synthesized isotropic pattern antenna can be realized by arrangeing $\lambda$/ 2 dipole along each x, y, z axis. The sleeve type $\lambda$/ 2 dipole antenna is used as a basic element for a proper impedance matching at feeding point and minimum mutual coupling effect between each element at 820 MHz - 895 MHz band. The total electric field intensity radiated from the designed isotropic pattern antenna is drived from the magnetic vector potential which is produced by each sleeve $\lambda$/ 2 dipole in the far field. This total electric field intensity is inversely proportional only to distance $\gamma$from the origin of coordinates, and pattern factor variation is less than 2.1dB. The measured value of total electric field variation depending on pattern factor is less then 2.8dB, and the difference between the calculated and measured value is 0.7dB.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.11
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• pp.995-1004
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• 2011

Object X-ray method commonly used for hole search in overset grids requires huge amount of time due to complicated vector calculations to search the cross-points as well as time-consuming hole search algorithm with respect to background grids. Especially, when the grid system is in motion relative to the background, hole points should be searched at every time step, leading to hung computational burden. To cope with this difficulties, this study presents an efficient hole search algorithm mainly designed to reduce hole searching time. To this end, virtual surface with reduced grid points is suggested and logical operators are employed as a classification algorithm instead of complicated vector calculations. In addition, the searching process is further accelerated by designating hole points in a row rather than discriminating hole points with respect to each background grid points. If there exists a relative motion, the present algorithm requires much less time because only the virtual surface needs to be moved at every time step. The hole searching time has been systematically compared for a few selected geometries.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.928-934
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• 2005

ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.

• BMB Reports
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• v.40 no.5
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• pp.656-661
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• 2007

In this report, we describe an optimized method for generation of ephA8 BAC transgenic mice expressing the lacZ reporter gene under ephA8 regulatory sequences. First, we constructed a targeting vector that carries a 1.2 kb ephA8 DNA upstream of its first exon, a lacZ expression cassette, a kanamycin cassette, and a 0.7 kb ephA8 DNA downstream of its first exon. Second, the targeting vector was electroporated into cells containing the ephA8 BAC and pKOBEGA, in which recombinases induce a homologous recombination between the ephA8 BAC DNA and the targeting vector. Third, the FLP plasmid expressing the Flipase was electroporated into these bacteria to eliminate a kanamycin cassette from the recombinant BAC DNA. The appropriate structures of the modified ephA8 BAC DNA were confirmed by Southern analysis. Finally, BAC transgenic mouse embryos were generated by pronuclear injection of the recombinant BAC DNA. Whole mount X-gal staining revealed that the lacZ reporter expression is restricted to the anterior region of the developing midbrain in each transgenic embryo. These results indicate that the ephA8 BAC DNA contains most, if not all, regulatory sequences to direct temporal and spatial expression of the lacZ gene in vivo.