• Title/Summary/Keyword: Won Su Lee

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Improvement of analytical method for pymetrozine in citrus fruits (감귤류 과일의 피메트로진 정량을 위한 분석법 개선)

  • Jeon, Jun-Ho;Chun, Su-Hyun;Kim, Min-Hyuk;Kim, Mi-Ok;Lee, Kwang-Won
    • Korean Journal of Food Science and Technology
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    • v.51 no.4
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    • pp.316-323
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    • 2019
  • It is difficult to analyze pymetrozine in citrus fruits using the hydromatrix method because of its low efficiency of purification and overlap of matrix and pymetrozine peaks. Liquid-liquid extraction can analyze pymetrozine in citrus fruits using dichloromethane. Since low pH interferes with the extraction of pymetrozine, the extracts of citrus fruits were maintained over pH 7.0 by adding borax buffer and 1 N NaOH in the improved method. According to the improved method, citrus fruits (such as lemon, lime, orange, tangerine, and grapefruit) were extracted and purified for HPLC-photo diode array analysis. The results of validation were as follows: $4.360{\mu}g/kg$ of limit of detection, $14.533{\mu}g/kg$ of limit of quantitation, and 0.007 mg/kg of method quantitative limit. Citrus fruits spiked with pymetrozine showed a recovery range from 71.8 to 83.7% and a coefficient of variation below 6%. Thus, the improved method can efficiently analyze pymetrozine in citrus fruits.

Does the Availability of Various Types and Quantity of Food Limit the Community Structure of the Benthos (Mollusks) Inhabiting in the Hard-bottom Subtidal Area? (먹이생물의 종류와 양이 암반 조하대 저서동물(연체동물) 군집구조 결정요소가 될 수 있는가?)

  • SON, MIN-HO;KIM, HYUN-JUNG;KANG, CHANG-KEUN;HWANG, IN-SUH;KIM, YOUNG-NAM;MOON, CHANG-HO;HWANG, JUNG-MIN;HAN, SU-JIN;LEE, WON-HAENG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.1
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    • pp.128-138
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    • 2019
  • Effects of feeding type and food resource availability on community structure of mollusks inhabiting hard-bottom subtidal areas were investigated. By following guidance from several references, mollusks observed in this study were divided into 5 groups according to feeding type - 1) grazing, 2) filter feeding, 3) deposit feeding, 4) omnivorous and 5) predation. The results showed that both grazing and filter feeders were the most numerous, explaining grazing type in the East Sea accounting for 47.9%, 32.6% in the South Sea and 29.6% for filter feeding, and filter feeding as a dominant feeding type in Yellow Sea accounting for 42.3%. Results of this study showed distinctive difference in community structure depending on mechanism of feeding type and geographical areas where sampling took place. With the results, attempts were made to understand whether community structure could be affected by feeding type or feeding availability and found out that community structure depended heavily on food resource availability. In the East Sea where marine algal density was high, the algal community in the forms of thick-leathery and sheet often occurred in water column with high transparency which provides proper environment for growth. In the South Sea where grazing and filter feeding types were predominated similarly, the algal density was high, but had the relative highest phytoplankton density. Whereas in the Yellow Sea showing the lowest algal biomass compared to the one in the East and the South Sea, and phytoplankton density was similar to those. It might be a adequate environment for filter feeders than grazers. This study concluded that community structure of mollusks showing high abundance was present where food resource availability with types and quantity was high.

Double-processed ginseng berry extracts enhance learning and memory in an Aβ42-induced Alzheimer's mouse model (Aβ42로 유도된 알츠하이머 마우스 모델에서 이중 가공 인삼열매 추출물의 학습 및 기억 손실 개선 효과)

  • Jang, Su Kil;Ahn, Jeong Won;Jo, Boram;Kim, Hyun Soo;Kim, Seo Jin;Sung, Eun Ah;Lee, Do Ik;Park, Hee Yong;Jin, Duk Hee;Joo, Seong Soo
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.160-168
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    • 2019
  • This study aimed to determine whether double-processed ginseng berry extract (PGBC) could improve learning and memory in an $A\hat{a}42$-induced Alzheimer's mouse model. Passive avoidance test (PAT) and Morris water-maze test (MWMT) were performed after mice were treated with PGBC, followed by acetylcholine (ACh) measurement and glial fibrillary acidic protein (GFAP) detection for brain damage. Furthermore, acetylcholinesterase (AChE) activity and choline acetyltransferase (ChAT) expression were analyzed using Ellman's and qPCR assays, respectively. Results demonstrated that PGBC contained a high amount of ginsenosides (Re, Rd, and Rg3), which are responsible for the clearance of $A{\hat{a}} 42$. They also helped to significantly improve PAT and MWMT performance in the $A{\hat{a}} 42-induced$ Alzheimer's mouse model when compared to the normal group. Interestingly, ACh and ChAT were remarkably upregulated and AChE activities were significantly inhibited, suggesting PGBC to be a palliative adjuvant for treating Alzheimer's disease. Altogether, PGBC was found to play a positive role in improving cognitive abilities. Thus, it could be a new alternative solution for alleviating Alzheimer's disease symptoms.

Meteorological Factors Associated with the Number of Emergency Room Patients with Wrist-Cutting Behavior (손목자해로 응급실에 내원한 환자수와 기후인자와의 관련성)

  • Han, Jae Hyun;Suh, Seung Wan;Cho, Gyu Chong;Kim, Jung Mi;Seo, Hong Taek;Jung, Yu Jin;Seong, Su Jeong;Hwang, Jae Yeon;Lee, Won Joon
    • Korean Journal of Psychosomatic Medicine
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    • v.28 no.2
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    • pp.161-167
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    • 2020
  • Objectives : Although the seasonality of suicide is a well-known phenomenon, little is reported about the seasonality of non-suicidal self-injury. The purpose of this study was to identify the seasonality of wristcutting behavior and to examine its relationship with meteorological factors. Methods : To identify the presence of seasonality, we investigated whether there was a difference in the average number of visits per month to an emergency room (ER) of an urban hospital for 226 patients with wrist-cutting behavior enrolled between December 2014 and May 2019. To ascertain significant meteorological factors, we used the multiple Poisson regression using generalized additive model with time, monthly temperature, monthly sunshine hour, and atmospheric pressure in the prior month as explanatory variables. Results : In males, the average number of monthly visits to the ER for wrist cutting behavior differed by month and was the highest in September (male : p=0.048, female : p=0.21, total : p=0.28). As a result of multiple regression analysis, the average number of patients admitted to the ER for wrist cutting behavior was related to the interaction between atmospheric pressure in the prior month and temperature in males (p=0.010), and showed a positive correlation with sunlight in females [p=0.044, β=4.70×10-3, 95% CI=(1.19×10-4, 9.27×10-3)]. Conclusions : Wrist cutting behavior shows seasonality in male, which is associated with changes in meteorological variables.

Hydrograph Separation and Flow Characteristic Analysis for Observed Rainfall Events during Flood Season in a Forested Headwater Stream (산지계류에 있어서 홍수기의 강우사상에 대한 유출수문곡선 분리 및 특성 분석)

  • Nam, Sooyoun;Chun, Kun-Woo;Lee, Jae Uk;Kang, Won Seok;Jang, Su-Jin
    • Korean Journal of Ecology and Environment
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    • v.54 no.1
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    • pp.49-60
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    • 2021
  • We examined the flow characteristics by direct runoff and base flow in a headwater stream during observed 59 rainfall events of flood season (June~September) from 2017 to 2020 yrs. Total precipitation ranged from 5.0 to 400.8 mm, total runoff ranged from 0.1 to 176.5 mm, and runoff ratio ranged from 0.1 to 242.9% during the rainfall events. From hydrograph separation, flow duration in base flow (139.3 days) was tended to be longer than direct runoff (78.3 days), while the contribution of direct runoff in total runoff (54.2%) was greater than base flow (45.8%). The total amount and peak flow of direct runoff and base flow had the highest correlation (p<0.05) with total precipitation and duration of rain among rainfall and soil moisture conditions. Dominant rainfall events for the total amount and peak flow of base flow were generated under 5.0~200.4 and 10.5~110.5 mm in total precipitation. However, when direct runoff occurred as dominant rainfall events, total amount and peak flow were increased by 267.4~400.8 and 169.0~400.8 mm in total precipitation. Therefore, the unique aspects of our study design permitted us to draw inferences about flow characteristic analysis with the contribution of base flow and/or direct runoff in the total runoff in a headwater stream. Furthermore, it will be useful for the long-term strategy of effective water management for integrated surface-groundwater in the forested headwater stream.

The Influence of Soil Characteristics and Sweet Potato (Ipomoea batatas L.) Varieties on Fiber Content (재배지역 토양특성과 고구마 품종의 섬유질 함량 차이)

  • Park, Won;Chung, Mi Nam;Lee, Hyeong-Un;Kim, Tae Hwa;Kim, Su Jung;Nam, Sang Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.67 no.3
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    • pp.172-179
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    • 2022
  • Sweet potato varieties with high fiber content in the storage root have poor texture when steamed or roasted. This study investigates the difference in fiber content among sweet potato varieties by soil and climate. The average fiber content of 'Hogammi', 'Sodammi', 'Pungwonmi', 'Danjami', and 'Jinyulmi' cultivars from the samples collected at farms in Haenam, Muan, and Unbong, Korea were 95.71, 66.73, 44.55, 40.55, and 38.53 mg/100g FW, respectively. There was no significant difference between site-specific conditions and varieties. Based on the degree of visual fibrousness, 'Hogammi' has an average of 3.6-4.0 with many thick stringy fibers. The fiber content of the 'Hogammi' cultivar was measured across 19 sites representing the main sweet potato growing regions of Korea. The fiber content was between 115.82 and 114.6 mg/100g in Haenam 2 and Boryeong 1, and 87.46 mg/100g in Hamyang. However, the fiber content at the remaining 16 sites was within the range of 94.63-108.52 mg/100g, although there were some site-level differences. The fiber content of the sweet potato storage roots were positively correlated with soil phosphorus (R2 = 0.58**), organic matter (R2 = 0.52*), and pH (R2 = 0.51*), which had a significance of 1% and 5%. The fiber content of sweet potato storage roots was found to have increased with increasing phosphorus content, organic matter and pH in the soil. However, there was no correlation with the amount of precipitation, days of precipitation and hours of sunshine with the fiber content of sweet potato at the selected sites.

A Basis Study on the Optimal Design of the Integrated PM/NOx Reduction Device (일체형 PM/NOx 동시저감장치의 최적 설계에 대한 기초 연구)

  • Choe, Su-Jeong;Pham, Van Chien;Lee, Won-Ju;Kim, Jun-Soo;Kim, Jeong-Kuk;Park, Hoyong;Lim, In Gweon;Choi, Jae-Hyuk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.6
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    • pp.1092-1099
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    • 2022
  • Research on exhaust aftertreatment devices to reduce air pollutants and greenhouse gas emissions is being actively conducted. However, in the case of the particulate matters/nitrogen oxides (PM/NOx) simultaneous reduction device for ships, the problem of back pressure on the diesel engine and replacement of the filter carrier is occurring. In this study, for the optimal design of the integrated device that can simultaneously reduce PM/NOx, an appropriate standard was presented by studying the flow inside the device and change in back pressure through the inlet/outlet pressure. Ansys Fluent was used to apply porous media conditions to a diesel particulate filter (DPF) and selective catalytic reduction (SCR) by setting porosity to 30%, 40%, 50%, 60%, and 70%. In addition, the ef ect on back pressure was analyzed by applying the inlet velocity according to the engine load to 7.4 m/s, 10.3 m/s, 13.1 m/s, and 26.2 m/s as boundary conditions. As a result of a computational fluid dynamics analysis, the rate of change for back pressure by changing the inlet velocity was greater than when inlet temperature was changed, and the maximum rate of change was 27.4 mbar. This was evaluated as a suitable device for ships of 1800kW because the back pressure in all boundary conditions did not exceed the classification standard of 68mbar.

Alteration Yield Traits of Sweetpotato (Ipomoea batatas L.) Cultivars Suitable for Paddy Cultivation (논 재배에 적합한 가공용 고구마 품종 선발을 위한 수량성 평가)

  • Won Park;Mi Nam Chung;Hyeong-Un Lee;Tae Hwa Kim;Su Jung Kim;Sang Sik Nam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.1
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    • pp.27-33
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    • 2023
  • Several domestically consumed sweetpotato derivatives, such as sweetpotato starch for processing raw materials, frozen and refrigerated paste, and powder are dependent on imports. This study was conducted to examine the suitability of sweetpotato cultivars of twelve varieties (powdery-type and viscous type) cultivated in paddy fields, for use in starch, chips, dried products, and beverages. The two-year average yield results of the four cultivars suitable for starch (in order of highest to lowest yield) was as follows: Gogeonmi (4,018 kg/10a); Daeyumi (3,615 kg/10a); Jinhongmi (3,426 kg/10a); Singeonmi (2,837 kg/10a). The starch content was 20.2%, 18.2%, 21.2%, and 20.6% in Daeyumi, Gogeonmi, Singeonmi, and Jinghongmi, respectively. The total amount of starch was higher in Daeyumi (730 kg/10a) and Gogeonmi (731 kg/10a) than that in Singeonmi and Jinghongmi. The yield of Pungwonmi and Shinjami were 4,443 and 3,602 kg/10a, respectively. Powdery-type sweetpotatoes (Daeyumi and Gogeonmi) showed the low decay rates of all cultivars (0.8 and 0%, respectively). The yield of the storage root formation and storage root swelling stages by water-logging treatment decreased by 16.5% and 15.4% for Pungwonmi, and by 17.2% and 10.0% for Shinjami. Drainage management of paddy fields is necessary to reduce the damage caused by water-logging. Our results suggest that cultivation of sweetpotato varieties suitable for processing raw materials in paddy fields will enable stable yields of sweetpotato with a high starch content.

Biological Activity of Mixed Extracts of Acanthopanax senticosus and Citrus unshiu Fermented with Bovista plumbea for Inner Beauty (이너뷰티 소재로서의 생물전환된 가시오가피-진피 혼합 추출물의 생물학적 활성)

  • Eun Jeong Kim;So Yeon Kim;Su Yeon Kang;Yung Choon Yoo;Taek Joon Yoon;Gye Won Lee;Young Ho Cho
    • Journal of Life Science
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    • v.33 no.7
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    • pp.555-564
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    • 2023
  • In this study, the anti-oxidant activity, elastase inhibitory activity, and skin moisturizing effect of mixed extracts of Acanthopanax senticosus and Citrus unshiu fermented with Bovista plumbea (B-MEAC) were evaluated to verify the availability as a material for inner beauty. The DPPH radical scavenging activity of B-MEAC was showed in a dose-dependent manner (SC50=156.1±0.82 ㎍/ml). Also, B-MEAC inhibited the elastase activity in a concentration-dependent manner (p<0.001). To study the effect of B-MEAC on mouse skin hydration, skin moisture content and transepidermal water loss (TEWL) measured. As a result, skin moisture content increased (p<0.001) and TEWL decreased (p<0.01) compared to the dry-induced control group. The effect on the change of collagen fibers in the dry-induced mouse skin was examined through Masson's trichrome staining. In the group administered with B-MEAC, the amount of collagen relatively increased compared to the control group, and the intensity of blue color increased. The effect on the moisturizing function of the dry-induced mouse skin was examined by Western blot method. In the group administered with B-MEAC, the expression of matrix metalloproteinase-1 (MMP-1) protein decreased compared to the control group. In addition, the expression level of collagen1A1 (COL1A1), hyaluronan synthase-2 (HAS2), filaggrin, and aquaporin-3 (AQP3) recovered (p<0.001). Therefore, these results suggest the potential of B-MEAC as a skin hydration agent for inner beauty.

Gut Microbiome and Gut Immunity in Broiler Chickens Fed Allium hookeri Root Powder from Day 10 to 28 (육계 사료 내 삼채뿌리분말 첨가가 장내 미생물 및 장관면역에 미치는 영향)

  • Woonhak Ji;Inho Cho;Sang Seok Joo;Moongyeong Jung;Chae Won Lee;June Hyeok Yoon;Su Hyun An;Myunghoo Kim;Changsu Kong
    • Korean Journal of Poultry Science
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    • v.50 no.3
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    • pp.171-185
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    • 2023
  • This study was conducted to investigate the effects of supplementation of Allium hookeri (AH) root powder on the gut microbiome, immunity, and health in broiler chickens fed experimental diets from d 10 to 28. A total of 60 10-day-old Ross 308 broilers were weighed and assigned to two dietary treatments with 5 birds per cage in a randomized complete block design based on body weight. The two experimental diets consisted of a control diet based on corn-soybean meal and the control diet supplemented with 0.3% AH root powder. All birds were fed ad libitum with experimental diets and water for 18 d. At 28 d, two birds near the median weight from each cage were selected for cecal content and small intestinal tissue sample collection. The addition of AH changed the gut microbiome by increasing probiotic candidate beneficial bacteria such as Enterococcaceae, Lactobacillaceae, Limosilactobacillus, Cuneatibacter, and Ruminoccoides. Regarding gut immunity, the supplementation of AH resulted in changes in intestinal immune cells, including reduced CD3+CD4+ T cells, which are a type of helper T cell, in the small intestine of birds (P=0.049). Additionally, there was a tendency to increase the expression of antioxidant function-related gene such as GPX2 (P=0.060), but no significant changes were observed in cytokines such as IL1b, IL6, and IL10. Overall, the addition of AH root powder may have positive effects on the microbiome of the chickens. This may help promote gut health in broiler chickens at the age of d 10 to 28.