• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1109-1117
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• 2014

Chlorogenic acid and hydroxylcinnamoyl shikimates are major dietary phenolics as well as antioxidants, with recently discovered biological, activities including protection against chemotheraphy side effects and prevention of cardiovascular disease and cancer. Certain fruits and vegetables produce these compounds, although a microbial system can also be utilized for synthesis of chlorogenic acid and hydroxylcinnamoyl shikimates. In this study, we engineered Escherichia coli to produce chlorogenic acid and p-coumaroyl shikimates from glucose. For the synthesis of chlorogenic acid, two E. coli strains were used; one strain for the synthesis of caffeic acid from glucose and the other strain for the synthesis of chlorogenic acid from caffeic acid and quinic acid. The final yield of chlorogenic acid using this approach was approximately 78 mg/l. To synthesize p-coumaroyl shikimates, wild-type E. coli as well as several mutants were tested. Mutant E. coli carrying deletions in three genes (tyrR, pheA, and aroL) produced 236 mg/l of p-coumaroyl shikimates.

• 식물병연구
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• 제7권2호
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• pp.69-79
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• 2001

Meloidogyne arenaria race 2 (59%) is widely distributed, followed by M. incognita race 1 (23%), and an unknown race of M. incognita (18 ％) in greenhouses in southern Korea. The key character to distinguish between M. arenaria and M. incognita is excretory pore in female head. When oriental melon, Cucumis melo L., grafted on Shintozoa (Cucurbit maxima x Cu. moschata) is transplanted in February in a plastic tunnel inside a greenhouse infested with M. arenaria, nematodes produced egg masses on roots at 40 days after transplanting and the soil juveniles (J2) population reach maximum in July to 3,817/100 ㎤. Juveniles are distributed relatively uniform over the 180-cm-wide row horizontally and the highest density occurs at 0-25 cm soil depth. For the control of root-knot nematodes, rice rotation, solarization, and soil addition treatments are the most effective (P=0.05); treatments reduce number of J2 over 90% and increase yield two times. Corn retation, fosthiazate, and soil drying treatment are moderately effective, while sesame and green onion rotations are not effective. The relationship between M. arenaria and yield of oriental melon is adequately described by a linear regression model. In the test with wild Cucumis genetic sources introduced from U.S.Dept. of Agriculture (USDA), one of C.heptadactylus, two of C.anguria, two of C. anguria var. longaculeatus, nine of C. metuliferus are resistant to both species of root-knot nematodes.

• Journal of Plant Biology
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• 제15권s호
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• pp.33-49
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• 1972

Recently two thousand year old several villages ruins discovered at Amsadong, Daeshimli and Hunbaukol near the Han River side in Korea. An investigation of the present flora of these three localities was done. There are 84 families including 419 species of vascular plants. The vegetations of flora in the localities of the Han River side are very similar. the forests are poorly developed and appear as the secondary vegetations. The reforest plants can be recognized as Pinus rigida, quercus acutissima, Q. serrata, Robinia pseudoacacia and Alnus hirta. Herbaceous plants of Miscanthus sacchariflorus and Phragmitis japonicus bind up the soiles of the Han River side. Populus nigra grows abundantly in the moist places of sand bars which are located on the river side south of Daeshimli and on the northern side of the Han River across from Hunbaukol. These three area are fully cultivated but still many primitive farm techniques are employed. Farm produce crops include rice, corn, barley wheat, other vegetables, and a few ornamental plants. In this study the author found flower color variations ranging from a dark blue to a pale blue, and chromosome number differences existing between the populations of Commelina communis. The dark blue flowered taxa chromosome number is 44 and in the pale blue taxa it is 72. The wild white flowered Chrysanthemum is Chrysanthemum zawadskii Herb. ssp. acutilobum and which has finely lobed leaves, and has 54 chromosome in the root tip cells.

• 동아시아식생활학회지
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• 제12권6호
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• pp.489-501
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• 2002

The list of monthly offering items of the Chunshin(薦新) ceremony, the service of offering the first food product of year to ancestors, shown in the Walryong(月令) in Oreaeui(五禮儀), the book written in early years of the Chosun dynasty, was studied and classified by food group. Six kinds of the cereals and grain products - barley, wheat, an early-ripening rice plant, a barnyard millet, and millet seed were offered. The meat products offered were wild geese, pheasants, swans, hares and dried-pheasants. Though not shown in e Walryong, roe deer and deer were offered from hunting trips in e winter. Eight kinds of sea-fish, six kinds of fresh-water first and six kinds of other seafood such as crab, octopus, and squid were offered. Ten kinds of vegetables: bamboo shoots, e99plants, cucumbers, etc. - were offered. Eleven kinds of tree fruits - cherries, apricots, Pears, etc., seven kinds of citrus fruits - tangerines, citrons, etc., seven kinds of nuts - chestnuts, pine-nuts, etc. and two kinds of melons - watermelons etc. - were offered. The hi인est variety in the offering items was the fruits category The remaining offering items included wine brewed from new rice. new brown seaweed, and green tea.

• 농업생명과학연구
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• 제45권5호
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• pp.91-96
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• 2011

We investigated the inhibitory effects of solvent extracts from Ailanthus altissima in A549 human lung cancer cell. A. altissima has been recognized as a traditional healthy food due to its various biological activities against hypertension, strokes, fever, pain, neuralgia, inflammation, and cancer effects. Recently, it has been reported that the extracts of various wild vegetables show strong anti-cancer properties by induction of apoptosis. However, the mechanisms of their cytotoxicity in human lung cancer cells have been poorly understood. The present study was investigated the effects of solvent extracts from A. altissima on cell growth and apoptosis on A549 human lung cancer cells. A treatment of A. altissima inhibited the growth of A549 cells in a dose-dependent manner by inducing apoptosis. Especially, the chloroform fraction showed the highest anti-cancer effect among five kinds of fractions. And also, induction of apoptosis by chloroform fraction were associated with down-regulation of Bcl-2, and up-regulation of pro-apoptotic Bax expression. From these results, A. altissima may have a therapeutic potential in human lung cancer cells and as a functional food.

• 현장농수산연구지
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• 제24권1호
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• pp.29-35
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• 2022

This study was conducted to investigate the seed storage method and seed germination characteristics of Synurus deltoides (Ait.) Nakai, a kind of wild vegetables. Several parameters affecting seed germination, such as germination temperature, chilling duration, imbibition duration, and storage method were evaluated. The seed germination of Synurus deltoides showed the highest germination rate (GR), promptness index (PI), germination performance index (GPI), mean daily germination (MDG) at 25℃. This implies that the optimum germination temperature was 25℃. And also, Seeds stored at -4℃, after 150 days showed relatively high germination rate about 98%. Therefore, The optimum germination temperature of Synurus deltoides was 25℃ and to increase the germination rate over 98% of Synurus deltoides, cold-wet storage 20 days treatments at 4℃ were effective in the improvement of seed germination.

• 한국식품영양학회지
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• 제36권4호
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• pp.274-285
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• 2023

This study was conducted to select representative agricultural products (4 types of fruits and 4 types of wild vegetables) in Chungju, define their sensual characteristics, derive suitable flavour-pairing and recipes for each ingredient, and use them as a cornerstone in the development of menus. For the experiment, 10 experts were selected to choose 8 representative agricultural products in Chungju, and 18 menus were selected through a flavour-pairing survey. A consumer panel (a total of 413 people, 105 in their 20s, 103 in their 30s, 103 in their 40s, and 102 in their 50s) for evaluating the characteristics of consumer preferences was selected. After the flavour-pairing survey 'sweet taste', 'light flavour', 'soft flavour', 'savoury flavour', 'familiar flavour', 'harmonious flavour', 'softness', and 'harmoniousness with food ingredients' were determined as drivers of liking, on the other hand, 'disturbance with food ingredients' and 'soybean fishy smell' were determined as drivers of disliking. The degree of consumer preference and overall acceptance were found to be related to the consumers' familiarity, suggesting that if a menu should be developed using unfamiliar local agricultural products, it should be configured with familiar recipes and seasoning methods.

• 한국식품조리과학회지
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• 제32권1호
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• pp.50-57
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• 2016

본 연구는 김부각(KB), 쑥부쟁이 나물부각(SB), 쑥부쟁이 나물김부각(NB)을 제조하여 품질특성을 조사하였다. NB는 쑥부쟁이 나물을 잘게 썰어 들깨 양념하여 김에 부착시켜 찹쌀풀을 발라 부각으로 제조 한 후 동결 건조하였다. KB보다 SB와 NB의 기름 흡유율이 높아 나물의 기름 흡수가 많았다. SB의 DPPH radical 소거능이 가장 높게 측정되었으며 KB 보다는 NB가 높게 측정되어 나물이 김보다 항산화능이 높았다. 저장기간이 길어질수록 수분함량은 증가하는 반면 최대하중, 항복응력 및 경도는 감소하는 경향이었다. 전반적인 기호도는 NB>KB>SB 순이었고, NB와 SB 간 유의한 차이가 있었다. $25^{\circ}C$에서 40일 저장한 NB의 산가 및 과산화물가는 점차 증가하는 경향이었으나, 산패의 기준에는 못 미쳤다. 따라서, $25^{\circ}C$ 이하에서 40일까지는 저장 가능할 것으로 판단되며, 쑥부쟁이뿐만 아니라 기능성이 입증된 다양한 나물을 첨가한 부각을 제조한다면 다양한 소비자 기호도를 충족시킬 수 있는 부각제조가 가능할 수 있을 것으로 본다.

• 한국식품과학회지
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• 제37권2호
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• pp.233-240
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• 2005

본 연구에서는 산채식물들을 이용한 새로운 기능성 소재의 개발에 우선하여 생산량과 소비량이 비교적 양호한 산채류 7종 즉, 물엉겅퀴, 쇠무릅, 울릉미역취, 섬고사리, 서덜취, 눈개승마 및 쇠비름을 선정하여 각 부위별 메탄올추출물을 제조한 후 이들의 항산화 활성을 검색하였다. 총 폴리페놀성 화합물의 함량은 $16.74-130.20{\mu}g/mg$으로 다양하게 나타났다. 섬고사리 잎과 울릉미역취 뿌리는 DPPH 와 ABTS+ 소거활성에서 각각 13.20과 $14.91{\mu}g/mL$. 43.93과 $29.08{\mu}g/mL$의 $RC_{50}$ 값을 보여 가장 높은 소거활성을 나타냈으며 또한 폴리페놀의 함량에 비례하여 소거활성이 증가하여 폴리페놀 함량과 free radical 소거활성은 깊은 연관성이 있음을 알 수 있었다. $H_{2}O_{2}$에 대한 소거활성은 섬고사리 잎과 뿌리 및 울릉미역취 뿌리에서 각각 72.83, 89, 99.62%로 높은 활성을 나타냈고 이러한 결과 역시 폴리페놀 함량과의 연관성을 나타냈다. 산채나물 추출물을 이용하여 linoleic acid에 대한 과산화 억제 효과를 ferric thiocyanate법으로 살펴본 결과, $100{\mu}g/mL$의 처리농도에서 모두 90% 이상의 산화억제 효과를 보였고, $10{\mu}g/mL$ 처리 농도에서는 울릉미역취 뿌리와 씨 그리고 서덜취 잎에서 각각 83.4, 87.4, 79.8%의 산학억제 효과를 보였다. Hydroxy radical에 의한 2-deoxy-D-ribose의 산화억제 효과는 $100{\mu}g/mL$의 처리농도에서 쇠무릎 잎과 씨, 물엉겅퀴 씨, 울릉미역취 씨는 116.9, 84.8, 94.3, 93.9%로 높은 저해율을 보였다. 것으로 나타났다. 그러므로 감마선 조사 및 저온저장($10^{\circ}C$)은 김밥재료 뿐만 아니라 김밥의 미생물 제어에 효과적인 것으로 확인되었다.와 비례하는 경향을 나타내었다. 또한 FA-swelling mica의 중금속 이온의 선택성은 Pb>Cu>Cd$\geq$Zn 순으로 나타났다.지 않았다.l years and a new type of transfer crane has been developed. Design concepts and control methods of a new crane will be introduced in this paper.and momentum balance was applied to the fluid field of bundle. while the movement of′ individual material was taken into account. The constitutive model relating the surface force and the deformation of bundle was introduced by considering a representative prodedure that stands for the bundle movement. Then a fundamental equations system could be simplified considering a steady state of the process. On the basis of the simplified model, the simulation was performed and the results could be confirmed by the experiments under various conditions.뢰, 결속 등 다차원의 개념에 대한 심도 깊은 연구와 최근 제기되고 있는 이론의 확대도 필요하다. 마지막으로 신뢰와 결속에 영향을

• 한국식품저장유통학회지
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• 제21권1호
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• pp.82-90
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• 2014

본 연구는 인공 재배 산채의 생산량 및 산채의 가공제품의 개발의 증대함에 따라 야생 및 재배 참취의 품질 특성을 비교하고자 재배 및 야생 참취의 건조방법에 따른 항산화성을 비교 분석하였다. 참취의 열수 추출물의 수율은 야생 참취에 비해 재배 참취에서 수율이 높았고, 블랜칭 건조 추출물의 수율이 높았다. 야생 참취 추출물의 총 폴리페놀과 플라보노이드의 함량은 재배 참취 추출물보다 높았으며, 건조방법에 따른 총 폴리페놀의 함량은 블랜칭 건조 추출물에서 높았고, 플라보노이드의 경우에는 생채 추출물에서 높았다. 참취의 열수 추출물의 전자공여능은 농도가 증가함에 따라 증가하였고, 블랜칭 건조의 열수 추출물에서 높은 전자공여능을 보였다. 참취 열수 추출물의 SOD 유사활성능은 추출물의 농도가 높아질수록 증가하였으며, 생채 열수 추출물에서 다른 추출물에 비해 높았다. pH 1.2에서의 아질산염 소거능은 야생 및 재배의 생채, 블랜칭건조, 생채건조 열수 추출물 순으로 높았다. Xanthine oxidase 저해활성은 열수 추출물의 농도가 증가할수록 저해효과가 높았으며, 생채건조 추출물에서 가장 높았다. Tyrosinase 저해활성과 환원력은 농도가 증가함에 따라 블랜칭 건조열수 추출물에서 높게 나타났다. 따라서 참취의 재배 및 건조 방법에 따른 열수 추출물은 항산화성이 우수하여 천연항산화 소재 및 기능성 식품소재로서의 활용 가능한 약용식물자원이며, 이를 활용한 가공산업 발전의 기초자료가 될 것으로 생각된다.