• Journal of Microbiology and Biotechnology
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• v.29 no.8
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• pp.1255-1265
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• 2019

To investigate the diversity of gastrointestinal microflora and lignocellulose-degrading enzymes in wild Asian elephants, three of these animals living in the same group were selected for study from the Wild Elephant Valley in the Xishuangbanna Nature Reserve of Yunnan Province, China. Fresh fecal samples from the three wild Asian elephants were analyzed by metagenomic sequencing to study the diversity of their gastrointestinal microbes and cellulolytic enzymes. There were a high abundance of Firmicutes and a higher abundance of hemicellulose-degrading hydrolases than cellulose-degrading hydrolases in the wild Asian elephants. Furthermore, there were a high abundance and a rich diversity of carbohydrate active enzymes (CAZymes) obtained from the gene set annotation of the three samples, with the majority of them showing low identity with the CAZy database entry. About half of the CAZymes had no species source at the phylum or genus level. These indicated that the wild Asian elephants might possess greater ability to digest hemicellulose than cellulose to provide energy, and moreover, the gastrointestinal tracts of these pachyderms might be a potential source of novel efficient lignocellulose-degrading enzymes. Therefore, the exploitation and utilization of these enzyme resources could help us to alleviate the current energy crisis and ensure food security.

• Journal of Ecology and Environment
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• v.43 no.3
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• pp.297-304
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• 2019

Background: The growing wild boar population has become a social issue and its feeding characteristics could affect the physical condition and the plant species composition in the South Korean forests. We aimed to reveal the preference of the wild boar on forest type and site condition as feeding grounds in two cool-temperate forested national parks, Odaesan and Seoraksan, in order to provide information to manage the growing population. Results: The 75 plots (53.6%) out of 140 plots were used as feeding grounds by the wild boar, implying a considerably large population. Especially, the observation frequency as feeding ground was the highest in Quercus forests (73.3%), and it was significantly more preferred than deciduous forest type (44.2%) and coniferous forest type (32.4%) (${\chi}^2=17.591$, p < 0.001). Significantly more and deeper pits were found in Quercus forests. Moreover, high elevation and gentle slope ridge were relatively preferred regardless of forest distribution. Conclusions: South Korean forests are growing qualitatively and quantitatively. Particularly, Quercus forest area has increased markedly, while coniferous forest area has decreased. Since the Quercus forest provides rich food sources for the wild boar, the enlargement of this forest type is expected to increase the wild boar population. The forests located at high elevations have high species diversity, and it is expected that these forests will be greatly affected by the increase in the wild boar population as preferred feeding grounds.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.87-99
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• 2010

Genetic diversity and relationships within and among Korean, Japanese and Chinese Jilin provincial wild soybeans based on SSR markers were evaluated to enlarge genetic variation in soybean breeding in the future. A total of 184 wild soybeans including 67 Korean, 71 Japanese and 46 Chinese Jilin provincial wild soybeans were analyzed to evaluate genetic diversity and relationships based on 23 SSR markers. Korean and Japanese wild soybeans were obtained from National Agrobiodiversity Center, Korea, and Biological Resource Center in Lotus and Glycine, Frontier Science Research Center, University of Miyazaki, Japan, respectively. Chinese wild soybeans were collected from Jilin province, China. Twenty three SSR markers generated a total of 964 alleles with an average of 41.9 alleles per marker. Number of alleles ranged from 23 (Satt635) to 56 (Satt157). Genetic diversity (PIC value) of 184 wild soybeans ranged from 0.880 to 0.968 with an average of 0.945. Number of alleles for Korean, Japanese and Chinese Jilin provincial wild soybeans was 513 with an average of 22.3, 511 with an average of 22.2, and 312 with an average of 13.6 per marker, respectively. PIC value for Korean, Japanese and Chinese Jilin provincial wild soybeans was similar with an average of 0.905, 0.897, and 0.850, respectively. Cluster analysis based on genetic distances estimated by SSR markers classified wild soybeans into 3 clusters. Cluster I included only Chinese Jilin provincial wild soybeans. Cluster II included most of Japanese wild soybeans including 5 Korean wild soybeans. Cluster III included most of Korean wild soybeans including 6 Japanese and 1 Chinese Jilin provincial wild soybeans. Cluster I was not subclassified, but cluster II and III were subclassified into various groups. Genetic distance evaluated by SSR markers between Korean and Japanese wild soybeans was closer than that of between Korean and Chinese Jilin provincial, and between Japanese and Chinese Jilin provincial wild soybeans.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.587-593
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• 1995

Growth characteristics and aromatic constituents of wild and domesticated Codonopsis lanceolata grown in different regions were investigated to find the best cultural condition for quality improvement. The results were as followings: Air temperature of wild region was lower to 4~8 and soil temperature was also lower to 3 compared with domesticated region. Soil of wild region had about three times richer organic matter with lower pH. Chromosome number of wild and domestcated species was 2n=16, ovary number was 3, but seed number of wild species was 132 vs domesticated showing 62. Recovery yield of wild species grown wild region had 0.55%, besides that, the wild species grown in domesticated region had 0.34. But the domesticated species in wild and domesticated regions was the same as 0.28. Inorganic element contents of domesticated region showed much higher than that of wild region. Major aromatic constituents were specified in wild Codonopsis lanceolata as dimethyl benzene, 3-ethyl-5-2-(ethylbutyl)-octadecane, benzaldehyde, 14, 14-dimethyl-hexadecanoate, methylhexadecenoate of which was not detected in domesticated species.

• Applied Biological Chemistry
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• v.51 no.1
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• pp.24-37
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• 2008

Yeast with excellent ferment ability was isolated and selected from wild grape to manufacture wild grape wine. Wild grape wine by SMR-3 isolated from wild grape was better than other strains in quality, such as high alcohol content and low acidity, residual sugar, organic acid and fusel oil content. Fermentation condition was optimized to manufacture wild grape wine with response surface methodology using isolated SMR-3 as an alcohol fermentation strain. As a result of culture conditions, 10.61% of alcohol content was expected under the conditions of $21.91^{\circ}C$ fermenting temperature, $21.48^{\circ}brix$ of initial sugar content, and 14.65 day of fermentation time. Residual sugar content showed the lowest value at $24.48^{\circ}C$ fermentation temperature, $12.78^{\circ}brix$ of initial sugar content, and 9.02 day fermentation time. The highest level of sensory evaluation was found at $20.23^{\circ}C$ fermentation temperature, $25.30^{\circ}brix$ of initial sugar content, and 5.94 day fermentation time. Ethyl alcohol was the main alcohol component in wild grape wine and fusel oil in wild grape wine was hardly detected; thus, the quality of wild grape wine was considered excellent. The optimal fermentation conditions of wild grape wine was superimposed by deriving a regression equation for alcohol content, fusel oil, ethyl alcohol content, and overall palatability for each variable of wild grape wine. Hence, the optimal fermentation conditions are estimated to be: fermentation temperature $24{\sim}28^{\circ}C$, initial sugar content $20{\sim}24^{\circ}brix$, and fermenting time $12{\sim}14$ days.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.1
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• pp.118-127
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• 2015

In this study, chiffon cakes were prepared with wild grape powder. Different amounts of the wild grape powder were tested (3%, 6%, 9%, and 12%) to determine the optimal amount. Rice chiffon cake was evaluated for quality characteristics, antioxidant properties, and sensory evaluation. The specific gravity of control batter was 0.48 g/mL, and it decreased with increasing amounts of wild grape powder, whereas viscosity showed the reverse effect. The pH of batter and cake decreased with increasing amounts of wild grape powder. The moisture content significantly increased with increasing amounts of wild grape powder, and the water activity of cakes was not significantly different between samples from 0.905~0.908. For color, lightness and yellowness deceased with increasing amounts of wild grape powder, whereas redness showed the reverse effect. For texture, hardness, fracturability, gumminess, and chewiness significantly increased with increasing amounts of wild grape powder. Total polyphenol content, DPPH radical scavenging activity, and ABTS radical scavenging activity significantly increased with increasing amounts of wild grape powder. In the sensory evaluation, the control sample showed the highest scores for color, flavor, softness, and overall acceptability. Based on these results, wild grape powder could be considered as a functional material, and 6% wild grape powder is the optimum level for manufacture of rice chiffon cake.

• Food Quality and Culture
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• v.2 no.2
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• pp.67-72
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• 2008

The volatile flavor compounds of wild and cultivated chamnamul (Pimpinella brachycarpa), an aromatic medicinal plant, were isolated via the simultaneous distillation extraction method and analyzed by GC and GC-MSD. From the oils of the wild chamnamul, 56 volatile flavor compounds were identified, and the major constituents were found to be sabinene (58.37 ppm) and germacrene-D (45.73 ppm). From the oils of cultivated chamnamul, 36 volatile flavor compounds were identified--the major constituents were identified as $\beta$-selinene (38.41 ppm) and myrcene (12.76 ppm).

• Journal of the Korean Statistical Society
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• v.24 no.2
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• pp.551-562
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• 1995

We consider the iterated bootstrap method in regression model with heterogeneous error variances. The iterated wild bootstrap confidence intervla of the mean response is considered. It is shown that the iterated wild bootstrap confidence interval has coverage error of order $n^{-1}$ wheresa percentile method interval has an error of order $n^{-1/2}$. The simulation results reveal that the iterated bootstrap method calibrates the coverage error of percentile method interval successfully even for the small sample size.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.571-583
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• 2009

Lam Dong Province of Vietnam has an exceptional diversity of orchids and herbs. At least 920 herb species are widely mentioned in Vietnamese traditional medicinal literature and over 239 Lam Dong wild orchid species are used for attention under the Conservation Vietnamese wild orchids projects. In collaboration with Department of Plant Biotechnology, Dongguk University - South Korea working under supported ODA funds from Korea government (MIFAFF), we will consider how to collecting and preserving these plants in situ or conservating in vitro as a genetic resource.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.213-214
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• 2012