• Food Science of Animal Resources
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• v.31 no.6
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• pp.952-959
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• 2011

A new bacteriocin-producing lactic acid bacteria (LAB) which has been isolated from kimchi was identified as Pediococcus damnosus by use of API kit and 16S rDNA sequencing, and designated as P. damnosus JNU 534. The bacteriocin produced by P. damnosus JNU 534 markedly inhibited the growth of some of LAB and Listeria monocytogenes, whereas other pathogens including Gram negative bacteria were not susceptible. The production of bacteriocin started at the beginning of exponential phase and reached maximum activity at the early stationary phase. The bacteriocin was stable on the wide pH range of 2-9 and heat treatment up to $100^{\circ}C$ for 15 min. The antimicrobial compound was inactivated by treatments of proteolytic enzymes indicating its proteinaceous in nature. The bacteriocin was purified by 30% ammonium sulfate precipitation followed by hydrophobic interaction column and $C_{18}$ column chromatography. The estimated molecular weight of the bacteriocin using tricine SDS-PAGE was approximately 3.4 kDa and the identified N-terminal amino acid sequence was $NH_2$-ILLEELNV.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.1
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• pp.105-114
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• 2015

Even though the passengers' safety has priority on Passenger ship, there is no criterion on the evacuation performance related with human behavior. To uncover the potential problems of domestic criteria on safety of passenger ships, this study performed simulation ad analysis the evacuees safety before and after the Sewol's remodelling. It is clear that the assembly stations of both before and after Sewol remodelling have sufficient areas to satisfy the domestic criterion by simply human- body's-area, but those are failed to assemble all the passengers by the simulation tool that reflects the human behaviors' characteristics. For the healing angle as 0 degree, and the criteria of SOLAS, it was found out that all the passengers can safely evacuate from each cabins to the embarkation stations for both of the before-and-after the Sewol remodelling. But for the healing angle as 20 degree, both of the before-and-after Sewol remodelling are evaluated as possible to make all the passengers evacuate for day scenario and impossible for night scenario of SOLAS criteria. And because of the worse conditions after the remodelling, the probabilities of Sewol(before) are showed wide band comparing to Naminoue (after).

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.174-183
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• 2001

During the rapid phase of gonadal development of the freshwater teleost, the catfish (Silurus asotus), the influence of hCG upon the inducement of final oocyte maturation and spawning was investigated electrophoretically and ultrastructurally. The electrophoretic patterns obtained were different in the presence and absence of some of the major or minor zones, because of the hormone level in catfish. The vitellogenin of hormone-treated fish was stained more intensively than that of sham-treated fish. These proteins showed some minor or main bands of egg extracts which migrated at positions corresponding to molecular weights of approximately 90,000. However, the thickness of electrophoretic band in molecular weight for hCG-treated fish was slightly lower than that for saline control. It seemed the plasma protein with molecular weight of approximately 45,000 in hCG-treated fish disappeared. In contrast to the control fish, the ovaries in the catfish treated with hCG shows a marked ultrastructural change under the electron microscope. No dilated profiles were seen in the granulosa cells of the mature oocyte before ovulation. After germinal vesicle breakdown (GVBD), the zona radiata interna (ZRI) becomes more compact, and there is a loss of all the processes from the pore canals. There is a wide space between the vitelline membrane and zona radiata. Also, during final maturation, the microvillar processes from the oocyte are seen no longer to penetrate deeply into the extracellular spaces of the overlying granulosa cells, and the reticulate patterns of the zona radiata interna becomes occluded, giving the zona radiata a more solid appearance. It has been possible to initiate 100% oocyte maturation in yolk granules and follicles in vivo by treatment with hCG and a high water temperature ($27^{\circ}C$). In hCG-treated fish, the percentages of successful artificial fertilization and hatching were maximal at 15 h after a single injection. It seems clear that a long acting preparation containing hCG can be successfully used in prespawning fish to advance the final events of gonadal maturation and initiate spawning. Further studies are necessary to evaluate the potential of hCG to either stimulate or inhibit the reproductive development of fish at other stages of the seasonal reproductive cycle.

• Korean Journal of Materials Research
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• v.20 no.12
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• pp.676-680
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• 2010

Most TCOs such as ITO, AZO(Al-doped ZnO), FTO(F-doped $SnO_2$) etc., which have been widely used in LCD, touch panel, solar cell, and organic LEDs etc. as transparent electrode material reveal n-type conductivity. But in order to realize transparent circuit, transparent p-n junction, and introduction of transparent p-type materials are prerequisite. Additional prerequisite condition is optical transparency in visible spectral region. Oxide based materials usually have a wide optical bandgap more than ~3.0 eV. In this study, single-phase transparent semiconductor of $SrCu_2O_2$, which shows p-type conductivity, have been synthesized by 2-step solid state reaction at $950^{\circ}C$ under $N_2$ atmosphere, and single-phase $SrCu_2O_2$ thin films of p-type TCOs have been deposited by RF magnetron sputtering on alkali-free glass substrate from single-phase target at $500^{\circ}C$, 1% $H_2$/(Ar + $H_2$) atmosphere. 3% $H_2$/(Ar + $H_2$) resulted in formation of second phases. Hall measurements confirmed the p-type nature of the fabricated $SrCu_2O_2$ thin films. The electrical conductivity, mobility of carrier and carrier density $5.27{\times}10^{-2}S/cm$, $2.2cm^2$/Vs, $1.53{\times}10^{17}/cm^3$ a room temperature, respectively. Transmittance and optical band-gap of the $SrCu_2O_2$ thin films revealed 62% at 550 nm and 3.28 eV. The electrical and optical properties of the obtained $SrCu_2O_2$ thin films deposited by RF magnetron sputtering were compared with those deposited by PLD and e-beam.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1295-1300
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• 2009

A 2,172-bp full-length gene (aga-F78), encoding a protease-resistant $\alpha$-galactosidase, was cloned from Rhizopus sp. F78 and expressed in Escherichia coli. The deduced amino acid sequence shared highest identity (45.0%) with an $\alpha$-galactosidase of glycoside hydrolase family 36 from Absidia corymbifera. After one-step purification with a Ni-NTA chelating column, the recombinant Aga-F78 migrated as a single band of ~82 and ~210 kDa on SDS-PAGE and nondenaturing gradient PAGE, respectively, indicating that the native structure of the recombinant Aga-F78 was a trimer. Exhibiting the similar properties as the authentic protein, purified recombinant Aga-F78 was optimally active at $50^{\circ}C$ and pH 4.8, highly pH stable over the pH range 5.0-10.0, more resistant to some cations and proteases, and had wide substrate specificity (pNPG, melidiose, raffinose, and stachyose). The recombinant enzyme also showed good hydrolytic ability to soybean meal, releasing galactose of $415.58\;{\mu}g/g$ soybean meal. When combined with trypsin, the enzyme retained over 90% degradability to soybean meal. These favorable properties make Aga-F78 a potential candidate for applications in the food and feed industries.

• Journal of the Korean Society for Nondestructive Testing
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• v.26 no.2
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• pp.84-89
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• 2006

A laser ultrasonic inspection system is a non-contact inspection device which generates and measures ultrasounds by using laser beams. A laser ultrasonic inspection system provides a high measurement resolution because the ultrasonic signal generated by a pulse laser beam has a wide-band spectrum and the ultrasonic signal is measured from a small focused spot of a measuring laser beam. In this paper, we have investigated the detection techniques of a surface-breaking crack by using the laser ultrasonic surface waves. A crack acts as a low pass filter whose cut-off frequency is lowered in proportion to the depth of a crack. And, the center frequency value of a spectrum is decreased in proportion to the depth of a crack. In this paper, we extracted the crack information by using the frequency attenuation from the normalized transfer function spectrum of a surface-breaking crack. Also, we effectively measured the crack depth by using the decreasing value of the center frequency from a crack passed ultrasonic signal. The proposed measuring techniques of crack depths provided more precise information than the amplitude measuring technique.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.219-223
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• 2001

Effects of hemoglobin (Hgb) concentration and degree of hydrolysis (DH) of Hgb on the separation of heme-iron were examined to produce highly enriched heme-iron from Hgb hydrolysate. Separation efficiency of Hgb hydrolysate with different DH was studied at wide pH range (pH $1.0{\sim}11.0$). Separation efficiency expressed as heme-iron/peptide ratio increased with decreasing Hgb concentration. When 5% Hgb (pH 10.0) was hydrolyzed using commercially available Esperase for 5 h at $50^{\circ}C$, DH was 25%. The precipitation of heme-iron-enriched peptides were remarkably high at pH range $3{\sim}6$. Optimal pH range for heme-iron with high heme-iron/peptide ratio shifted to acidic pH with increasing DHs of Hgb. The enriched heme-iron fraction in the precipitates showed a single band through urea-SDS-PAGE, with a molecular mass of 1 kDa. In the dry heme-iron product produced in a pilot bioreactor, content of heme-iron and heme-iron/peptide ratio were 27.1 and 38.7%, respectively, and production yield was 9.3%.

• Journal of Fisheries and Marine Sciences Education
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• v.11 no.2
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• pp.203-215
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• 1999

Floating debris was recorded from a training ship, Kaya, of Pukyong National University with about 12 knots speed at July 19~21, 1999 and July 24~26. The sampled area is the Yellow Sea (YS) connected from Cheju Is. of Korea to Qindao harbor of China, divided into 88 unit segments on survey routes. Debris fabrication materials were categorized with 6 items using the following; man-made or natural wood items, paper and cardboard, nylon netting and rope, styrofoams, vinyl and plastics, floating metal and glass containers. All identified items within a $120{\pm}20m$ wide band were recorded but ignored if beyond this boundary. The results of distribution of floating debris in the area are as follows: 1. The greatest quantities of marine debris discovered during the survey were $184pieces\;per\;km^2$ near Qindao harbor. The next polluted areas, Sohuksan islands, positioned in the eastern part of YS were scattered in about $40pieces/km^2$ or so. 2. Among items, woods, paper and cardboard, vinyl and plastics were found out near Qindao Coast of China. On the other hand, there were many styrofoams, nylon netting and rope near the area of Sohuksan islands in the eastern part of YS. 3. The distribution densities of western part of YS near Qindao are varied with largely range in items and quantities but those of the eastern part, near Sohuksan islands were nearly constant. 4. Styrofoams were composed of 63% among all debris in YS, next paper and cardboard 15%, and wood item, vinyl and plastics each 10%. Therefore, marine debris of YS is revealed that distribution characteristics and composition are very different in each area. These phenomena are concluded that they largely depend on the life styles in land and the utilities of the sea. And of items, styrofoams is dominated by the distribution pattern of YS. Furthermore, surveys are continuously being investigated, and from this it is hoped that a much wider coverage can be achieved, perhaps all sites of the YS and contributed to the finding sources, the stationary area and removal method of debris joined the flow patterns.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.17-18
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• 2011

Plasma in liquid phase has attracted great attention in the last few years by the wide domain of applications in material processing, decomposition of organic and inorganic chemical compounds and sterilization of water. The plasma in liquid is characterized by three main regions which interact each - other during the plasma operation: the liquid phase, which supply the plasma gas phase with various chemical compounds and ions, the plasma in the gas phase at atmospheric pressure and the interface between these two regions. The most complex region, but extremely interesting from the fundamental, chemical and physical processes which occur here, is the boundary between the liquid phase and the plasma gas phase. In our laboratory, plasma in liquid which behaves as a glow discharge type, is generated by using a bipolar pulsed power supply, with variable pulse width, in the range of 0.5~10 ${\mu}s$ and 10 to 30 kHz repetition rate. Plasma in water and other different solutions was characterized by electrical and optical measurements. Strong emissions of OH and H radicals dominate the optical spectra. Generally water with 500 ${\mu}S/cm$ conductivity has a breakdown voltage around 2 kV, depending on the pulse width and the repetition rate of the power supply. The characteristics of the plasma initiated in ultrapure water between pairs of different materials used for electrodes (W and Ta) were investigated by the time-resolved optical emission and the broad-band absorption spectroscopy. The deexcitation processes of the reactive species formed in the water plasma depend on the electrode material, but have been independent on the polarity of the applied voltage pulses. Recently, Coherent anti-Stokes Raman Spectroscopy method was employed to investigate the chemistry in the liquid phase and at the interface between the gas and the liquid phases of the solution plasma system. The use of the solution plasma allows rapid fabrication of the metal nanoparticles without being necessary the addition of different reducing agents, because plasma in the liquid phase provides a reaction field with a highly excited energy radicals. We successfully synthesized gold nanoparticles using a glow discharge in aqueous solution. Nanoparticles with an average size of less than 10 nm were obtained using chlorauric acid solutions as the metal source. Carbon/Pt hybrid nanostructures have been obtained by treating carbon balls, synthesized in a CVD chamber, with hexachloro- platinum acid in a solution plasma system. The solution plasma was successfully used to remove the template remained after the mesoporous silica synthesis. Surface functionalization of the carbon structures and the silica surface with different chemical groups and nanoparticles, was also performed by processing these materials in the liquid plasma.

• Journal of the Korean association of regional geographers
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• v.2 no.2
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• pp.183-196
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• 1996

The purpose of this paper is to explore the possibility of automatic extraction of line feature from Satellite image. The first part reviews the relationship between spatial filtering and cartographic interpretation. The second part describes the principal operations of high frequency filters and their properties, the third part presents the result of filtering application to the SPOT Panchromatic image of the Chinju city. Some experimental results are given here indicating the high feasibility of the filtering technique. The results of the paper is summarized as follows: Firstly the good all-purposes filter dose not exist. Certain laplacian filter and Frei-chen filter were very sensitive to the noise and could not detect line features in our case. Secondly, summary filters and some other filters do an excellent job of identifying edges around urban objects. With the filtered image added to the original image, the interpretation is more easy. Thirdly, Compass gradient masks may be used to perform two-dimensional, discrete differentiation directional edge enhancement, however, in our case, the line featuring was not satisfactory. In general, the wide masks detect the broad edges and narrow masks are used to detect the sharper discontinuities. But, in our case, the difference between the $3{\times}3$ and $7{\times}7$ kernel filters are not remarkable. It may be due to the good spatial resolution of Spot scene. The filtering effect depends on local circumstance. Band or kernel size selection must be also considered. For the skillful geographical interpretation, we need to take account the more subtle qualitative information.