• Title/Summary/Keyword: Wi-gi

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Supercooling Pretreatment Improves the Shelf-Life of Freeze-Dried Leuconostoc mesenteroides WiKim32

  • Seul-Gi Jeong;In Seong Choi;Ho Myeong Kim;Ji Yoon Chang;Hae Woong Park
    • Journal of Microbiology and Biotechnology
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    • v.32 no.12
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    • pp.1599-1604
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    • 2022

  • Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

  • https://doi.org/10.4014/jmb.2209.09022 인용 PDF KSCI

농촌지역정보망 구성방안(Ⅲ)

  • Wi, O-Gi;Lee, Gyu-Tae
    • Digital Contents
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    • no.11 s.18
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    • pp.17-29
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    • 1994

  • 이 글은 '93 통신학술과제로 수행된 "농산물 유통개선을 위한 정보망 구성방안에 관한 연구"를 바탕으로 농촌정보화에 중점을 두어 재구성한 것이다.

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농촌지역정보망 구성방안

  • Wi, O-Gi;Lee, Gyu-Dae
    • Digital Contents
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    • no.9 s.16
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    • pp.21-30
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    • 1994

  • 이 글은 '93 통신학술과제로 수행된 "농산물유통개선을 위한 정보망 구성방안에 관한 연구"를 바탕으로 농촌정보화에 중점을 두어 재구성한 것이다.

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농촌지역정보망 구성방안(Ⅳ)

  • Wi, O-Gi;Lee, Gyu-Dae
    • Digital Contents
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    • no.12 s.19
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    • pp.22-38
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    • 1994

  • 이 글은 '93 통신학술과제로 수행된 "농산물 유통개선을 위한 정보망 구성방안에 관한 연구"를 바탕으로 농촌정보화에 중점을 두어 재구성한 것이다.

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