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Biocontrol of Peach Gummosis by Bacillus velezensis KTA01 and Its Antifungal Mechanism

  • Tae-An Kang;GyuDae Lee;Kihwan Kim;Dongyup Hahn;Jae-Ho Shin;Won-Chan Kim
    • Journal of Microbiology and Biotechnology
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    • v.34 no.2
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    • pp.296-305
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    • 2024
  • Peach tree gummosis is a botanical anomaly distinguished by the secretion of dark-brown gum from the shoots of peach trees, and Botryosphaeria dothidea has been identified as one of the fungal species responsible for its occurrence. In South Korea, approximately 80% of gummosis cases are linked to infections caused by B. dothidea. In this study, we isolated microbes from the soil surrounding peach trees exhibiting antifungal activity against B. dothidea. Subsequently, we identified several bacterial strains as potential candidates for a biocontrol agent. Among them, Bacillus velezensis KTA01 displayed the most robust antifungal activity and was therefore selected for further analysis. To investigate the antifungal mechanism of B. velezensis KTA01, we performed tests to assess cell wall degradation and siderophore production. Additionally, we conducted reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis based on whole-genome sequencing to confirm the presence of genes responsible for the biosynthesis of lipopeptide compounds, a well-known characteristic of Bacillus spp., and to compare gene expression levels. Moreover, we extracted lipopeptide compounds using methanol and subjected them to both antifungal activity testing and high-performance liquid chromatography (HPLC) analysis. The experimental findings presented in this study unequivocally demonstrate the promising potential of B. velezensis KTA01 as a biocontrol agent against B. dothidea KACC45481, the pathogen responsible for causing peach tree gummosis.

Mineral chemistry and texture of the zoned amphiboles of the amphibolites in the Hwanggangri area, the northeastern region of Ogcheon metamorphic belt, Korea (옥천변성대 북동부 황강리 지역내 앰피볼라이트에 나타나는 대상 각섬석의 광물화학 및 조직)

  • 유영복;권용완;김형식
    • The Journal of the Petrological Society of Korea
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    • v.9 no.2
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    • pp.99-118
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    • 2000
  • The variation of Na(A), K, Na(M4), A l O and Al(VI)+Fe3++Ti+Cr in the zonal amphiboles from the amphibolites of the Hwanggangri area indicates that the tschermakite-, edenite- and glaucophane substitutions are higher in the rim than in the core, in which actinolite changes to hornblende with going outward from core to rim. The contents of substitutional elements of hornblende~ of three samples@l29-2, M76-2, M78), which include diopside and greenish brown hornblende and are thought to represent the highest metamorphic grade, are lower than those of rim homblendes of the lower metamorphic grade and are higher than those of core actinolite that they conform to the middle domain in those of the whole amphiboles. Considerations about the origin of zonal amphiboles are as follows. Firstly, two samples(R102-1, R210-9) have the same amphibole composition like core is actinolitic hornblende, and rim is magnesian hastingsite although plagioclases such as albite(R102-1) and labradorite (R210-9) show the wide compositional difference. It is impossible to produce both albite and labradorite by one metamorphic event. Judging from this wide compositional difference, the existence of zonal amphiboles does not indicate the miscibility gap but is thought to be the result of the polymetamorphism. Secondly, the crystallographically sharp and gradational interfaces between actinolite and hornblende fonned in the amphibolites rgardless of the distance from the granite. In case of the samples(R210-9, M128, M130) having the sharp interface between two amphiboles, the plagioclase show the compositions produced at the low grade and the medium grade. Because such variable compositions of plagioclase indicates the overprinting of metamorphism of higher metamorphic grade than that of the formation of miscibility gap, it implies that zonal amphiboles were formed by polymetarnorphism. In case of the gradational interface between two amphiboles, this texture is also thought to be the effect of polymetamorphism from the fact that this texture mainly occur near the granite and from the consideration of the metamporphic grade. The relationship between the compositional variations of the amphiboles and the pressure types of metamorphism suggests that actinolitic core is considered to be grown by the metamorphism of medium pressure, while hornblende rim is shown to have genetic relations with the metamorphism of low pressure type.

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LOCA Analysis and Development of a Simple Computer Code for Refill-Phase Analysis (냉각재 상실사고 분석 및 재충진 단계해석용 전산코드 개발)

  • Ree, Hee-Do;Park, Goon-Cherl;Kim, Hyo-Jung;Kim, Jin-Soo
    • Nuclear Engineering and Technology
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    • v.18 no.3
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    • pp.200-208
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    • 1986
  • The loss of coolant accident based on a double-ended cold leg break is analyzed with the discharge coefficient (Ca) of 0.4. This analysis covers the whole transient period from the start of depressurization to the complete refilling of the core by using RELAP4/MOD6-EM and RELAP4/ MOD6-HOT CHANNEL for the system thermal-hydraulics and the fuel performance during the blowdown phase respectively, and RELAP4/MOD6-FLOOD and TOODEE2 during the reflood phase. A simple analytical method has been developed to account for the lower plenum filling by approximating steam-water countercurrent flows and superheated wall effects at the downcomer during the refill period. Based on the informations. at the time of EOB (end-of-bypass), the refill duration time and the initial reflooding temperature were estimated and compared with the results from the RELAP4/MOD6, resulting in a good agreement. In addition, some parametric studies on the EOB were performed. The form loss coefficient between upper head and upper downcomer was found to be sensitive to the occurrence of the spurious EOB. Appropriate form loss coefficients should be taken into account to avoid the flow oscillations at the downcomer. The analyses with the six and three volume core nodalizations, respectively, show much similar trends in the system thermal-hydraulic performance, but the former case is recommended to obtain good results.

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Genome Wide Expression Analysis of the Effect of Pinelliae Rhizoma Extract on Psychological Stress (반하(半夏)가 스트레스로 인한 생쥐의 뇌조직 유전자변화에 미치는 영향 연구)

  • Jeong, Jong-Hyo;Cho, Su-In;Song, Young-Gil;Kim, Ha-Na;Kim, Kyeong-Ok
    • Journal of Oriental Neuropsychiatry
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    • v.26 no.1
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    • pp.63-78
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    • 2015
  • Objectives: Pinelliae Rhizoma has traditionally been used as an anti-depressant in oriental medicine. This study is to investigate the effect of Pinelliae Rhizoma extract (PRe) on psychological stress in genome wild expression of mice. Methods: After giving physical stress to mice, PRe was orally administered with 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, their genome changes were observed by micorarray analysis method. The genome changes were analyzed by IMAGENE 4.0, TREEVIEW, FatiGo algorithems, BOND database, cytoscape program, etc. Results: 1. PRe administered group were remained at normal level; 60% of increase was shown in expressed genes by physical stress, and 65% of decrease was shown in expressed genes by psychological stress. 2. Genes with increased expression in control group that remained at a normal state in PRe administered group were involved with the gene of a cellular metabolic process on biological process, protein binding on molecular function, and cell part on cell composition. The pathway was found to be cytokin-cytokin receptor interaction. 3. Genes with decreased expression in control group that remained at a normal state in PRe administered group were involved with the gene of a cellular metabolic process on biologiacl detail and coupled ATPaes activity on molecular function. This gene related path was Ubiquintin mediated proteolysis etc. 4. Core node genes analyzed by protein interaction network were Vinculin, Cell sdivision cycle 42 homolog (S. cerevisiae) etc. They played an important role in maintaining cytoskeleton and controlling cell cycle. Conclusions: Several genes were up-regulated and down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice. When the interaction network information was analyzed, the recovery of the core node genes in PRe treated mice indicates that this final set of genes may be the effective target of PRe.

The Characteristics of Visualizing Hierarchical Information and their Applications in Multimedia Design (멀티미디어디자인에서 정보위계 표출방식과 그 활용에 관한 연구)

  • You, Si-Cheon
    • Science of Emotion and Sensibility
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    • v.9 no.spc3
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    • pp.209-224
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    • 2006
  • Hierarchy which is often named as the tree-structure is used to reduce complexity and show primitive structures of complicated information. This paper aims at explaining information-visualization methods using hierarchies in multimedia domains and prospecting the possible applications by examining how they affect the user's tasks involved in information-seeking activities. As a result, four types of information visualization methods named Treemap, Hyperbolic, Cone Tree and DOI Tree employed in multimedia domain, are presented and pros and cons of each method are explained in this paper. Another important part is defining the core tasks and other related-tasks in information-seeking activities, such as, overview, zoom, filter, details-on-demand, relate, history, and extract. Followings are major findings. Treemap uses 'overview' as the core task, which makes user to gain a overall meaning of the whole information cluster. Hyperbolic and DOI Tree apply 'Boom' task through the function of focus+context or by the function of meaningful scaling to magnify or downsize each node. Cone Tree, also, makes the information organizer to classify the patterns of information acquired in the process of users' information-seeking activities by using 'extract' task. Through this study, it is finally found out that the information-visualization methods using hierarchies in multimedia domains should incorporate the wide variety of functional needs related to users' information-seeking behaviors beyond the visual representation of information.

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A Study of Secondary Mathematics Materials at a Gifted Education Center in Science Attached to a University Using Network Text Analysis (네트워크 텍스트 분석을 활용한 대학부설 과학영재교육원의 중등수학 강의교재 분석)

  • Kim, Sungyeun;Lee, Seonyoung;Shin, Jongho;Choi, Won
    • Communications of Mathematical Education
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    • v.29 no.3
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    • pp.465-489
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    • 2015
  • The purpose of this study is to suggest implications for the development and revision of future teaching materials for mathematically gifted students by using network text analysis of secondary mathematics materials. Subjects of the analysis were learning goals of 110 teaching materials in a gifted education center in science attached to a university from 2002 to 2014. In analysing the frequency of the texts that appeared in the learning goals, key words were selected. A co-occurrence matrix of the key words was established, and a basic information of network, centrality, centralization, component, and k-core were deducted. For the analysis, KrKwic, KrTitle, and NetMiner4.0 programs were used, respectively. The results of this study were as follows. First, there was a pivot of the network formed with core hubs including 'diversity', 'understanding' 'concept' 'method', 'application', 'connection' 'problem solving', 'basic', 'real life', and 'thinking ability' in the whole network from 2002 to 2014. In addition, knowledge aspects were well reflected in teaching materials based on the centralization analysis. Second, network text analysis based on the three periods of the Mater Plan for the promotion of gifted education was conducted. As a result, a network was built up with 'understanding', and there were strong ties among 'question', 'answer', and 'problem solving' regardless of the periods. On the contrary, the centrality analysis showed that 'communication', 'discovery', and 'proof' only appeared in the first, second, and third period of Master Plan, respectively. Therefore, the results of this study suggest that affective aspects and activities with high cognitive process should be accompanied, and learning goals' mannerism and ahistoricism be prevented in developing and revising teaching materials.

Comparison Of CATHARE2 And RELAP5/MOD3 Predictions On The BETHSY 6.2% TC Small-Break Loss-Of-Coolant Experiment (CATHARE2와 RELAP5/MOD3를 이용한 BETHSY 6.2 TC 소형 냉각재상실사고 실험결과의 해석)

  • Chung, Young-Jong;Jeong, Jae-Jun;Chang, Won-Pyo;Kim, Dong-Su
    • Nuclear Engineering and Technology
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    • v.26 no.1
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    • pp.126-139
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    • 1994
  • Best-estimate thermal-hydraulic codes, CATHARE2 V1.2 and RELAP5/MOD3, hate been assessed against the BETHSY 6.2 tc six-inch cold leg break loss-of-coolant accident (LOCA) test. Main objective is to analyze the overall capabilities of the two codes on physical phenomena of concern during the small break LOCA i.e. two-phase critical flow, depressurization, core water level de-pression, loop seal clearing, liquid holdup, etc. The calculation results show that the too codes predict well both in the occurrences and trends of major two-phase flow phenomena observed. Especially, the CATHARE2 calculations show better agreements with the experimental data. However, the two codes, in common, show some deviations in the predictions of loop seal clearing, collapsed core water level after the loop seal clearing, and accumulator injection behaviors. The discrepancies found from the comprision with the experimental data are larger in the RELAP5 results than in the CATHARE2. To analyze the deviations of the two code predictions in detail, several sensitivity calculations have been performed. In addition to the change of two-phase discharge coefficients for the break junction, fine nodalization and some corrections of the interphase drag term are made. For CATHARE2, the change of interphase drag force improves the mass distribution in the primary side. And the prediction of SG pressure is improved by the modification of boundary conditions. For RELAP5, any single input change doesn't improve the whole result and it is found that the interphase drag model has still large uncertainties.

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Problems in defining public library users as viewed through the 'book-room' phenomenon in Pusan (도서원현상을 통해 본 공공도서관 이용자에 대한 인식의 문제)

  • 김영기
    • Journal of Korean Library and Information Science Society
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    • v.19
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    • pp.145-209
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    • 1992
  • This study, that is made by an on-the-spot survey and a library survey, is aimed at finding some problems in the recognition regarding public library users and suggesting the clue to solve the problems revealed in the way of having compared with 'Book-Room' and Public Library. Poor material was always a troublemaker in this study. Especially the part of 'Book-Room' phenomenon is wholly dependent upon a field survey, that is, interview with a 'book-room' clerks, gathering the advertising leaflets, a close observation on 'Book-Room', etc. There are little printed materials, much less precedent research theses. In the other parts of this paper, there are needed good statistical materials, but the nortion presented in the public library user statistics-for example pupil, students, housewives, old men, the generality of people, etc.- is so vague that it is insufficient and unhelpful for this study. In my judgment, with all these limitations, this article gets some results as follows : 1. 'Book-Room' is the cultural organization which is being operated by, for, of the community inhabitants and it takes on the character of movement - labor movement, cultural movement, and community movement. 2. The pioneer of the Book-Room is 'Yangso Hyupdong chohap' (which means good book- cooperative association) lasted from 1978 to the next year. 3. 'Arom'(means learning) Book-Room is the first real Book-Room at Seomyon district in Pusan established in 1987. 4. Book-Room can be classified by its character as 'Labor BookRoom' and 'Cultural Book-Room'. There are 10 Labor BookRooms and 4 Cultural Book-Rooms in Pusan. 5. Labor Book-Rooms organized themselves to 'Labor Book-Room conference'. 6. Book-Rooms have done their best to extort the needs and interests of the community inhabitants, but relatively public libraries do not, hence the majority of residents are neglected from the public library service. This is at Book-Rooms' back to have been spread so quickly. 7. There is a very large gap between the component ratio of community inhabitants by class and that of public library users by same criterion, that is, in the case of the former, the labor class goes beyond a half point, in the latter, the majority of public library user is composed of students and housewives. 8. Researchers and librarians have concentrated their attention and effort not upon whole residents in community but upon existing users at present time. They do not try to develope potential users. So, researchers' and librarians' recognition regarding the public library users needs to be changed. 9. The concept of 'core-user' may be the key to solve the problems related with public library users. For the purpose of ensuring the 'core-user', it is necessary to study the community and inhabitants.

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Derivation and verification of electrical resistivity theory for surrounding ground condition prediction of TBM (TBM 주변 지반상태예측을 위한 전기비저항 이론식 유도 및 검증)

  • Hong, Chang-Ho;Lee, Minhyeong;Cho, Gye-Chun
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.22 no.1
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    • pp.135-144
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    • 2020
  • Since the depth of tunneling with tunnel boring machine (TBM) becomes deeper and deeper, the expense for site investigation for coring and geophysical survey increases to obtain the sufficient accuracy. The tunnel ahead prediction methods have been introduced to overcome this limitation in the stage of site investigation. Probe drilling can obtain the core and borehole images from a borehole. However, the space in TBM for the probe drilling equipment is restricted and the core from probe drilling cannot reflect the whole tunnel face. Seismic methods such as tunnel seismic prediction (TSP) can forecast over 100 m ahead from the tunnel face though the signal is usually generated using the explosive which can affect the stability of segments and backfill grout. Electromagnetic methods such as tunnel electrical resistivity prospecting system (TEPS) offer the exact prediction for a conductive zone such as water-bearing zone. However, the number of electrodes installed for exploration is limited in small diameter TBM and finally the reduction of prediction ranges. In this study, the theoretical equations for the electrical resistivity survey whose electrodes are installed in the face and side of TBM to minimize the installed electrodes on face. The experimental tests were conducted to verify the derived equations.

A STUDY ON THE SAFETY ANALYSIS OF ROCK FILL DAM (1) (필댐의 안정성 해석 연구 (1))

  • HoWoongShon;DaeKeunLee
    • Journal of the Korean Geophysical Society
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    • v.6 no.3
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    • pp.165-177
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    • 2003
  • The purpose of this paper is to analyze the behavior and to study the safety evaluation of the Unmun Dam located in Cheongdo-Gun of GyeongBuk Province, Korea. For this purpose, soil analyses including boring data, geophysical surveys were conducted. In this paper, especially many geophysical methods were adopted to configure out the subsurface situation of dam. Applied geophysical methods were: 1) electric resistivity survey, 2) high frequency magnetotelluric (HFMT) survey, 3) ground penetrating radar (GPR) survey, 4) seismic refraction survey, 5) seismic cross-hole tomography survey, and 6) high frequency impedance (ZHF) survey. Each of geophysical surveys were analyzed and joint analyses between geophysical surveys were also performed to deduce the more reliable subsurface information of Dam by using the features and characteristics of each geophysical survey. Since many defects, such as gravel and weathered rock blocks in the dam core, and lots of amounts of leakage, by boring analyses were found, reinforcement by compaction grouting system (CGS) has been conducted in some range of dam. Some geophysical data and data of geotechnical gauges were also used to confirm the effects of reinforcement. Electric resistivity, EM, GPR, ZHF, seismic refraction and seismic tomography surveys show that left side of dam is weak, which means the possibility of existence of gravel, rock block, water and cavities in the core of dam. This result coincides with the boring data. Especially, electric survey after reinforcement shows that even the right side of the dam has been deformed by the strong pressure during the reinforcement itself. As a conclusion, some problems in the dam found. Especially, the dam near spillway shows the high possibility of leakage. It should be pointed out that only the left side of he dam has not a leakage problem. As a whole, the dam has problems of weakness, because of unsatisfactory construction. It is strongly recommended that highly intensive monitoring is required.

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