• Journal of Microbiology and Biotechnology
• /
• v.17 no.10
• /
• pp.1727-1732
• /
• 2007

Phenylacetic acid (PAA) is produced by many bacteria as an antifungal agent and also appears to be an environmentally toxic chemical. The object of this study was to detect PAA using Pseudomonas putida harboring a reporter plasmid that has a PAA-inducible promoter fused to a green fluorescent protein (GFP) gene. Pseudomonas putida KT2440 was used to construct a green fluorescent protein-based reporter fusion using the paaA promoter region to detect the presence of PAA. The reporter strain exhibited a high level of gfp expression in minimal medium containing PAA; however, the level of GFP expression diminished when glucose was added to the medium, whereas other carbon sources, such as succinate and pyruvate, showed no catabolic repression. Interestingly, overexpression of a paaF gene encoding PAA-CoA ligase minimized catabolic repression. The reporter strain could also successfully detect PAA produced by other PAA-producing bacteria. This GFP-based bioreporter provides a useful tool for detecting bacteria producing PAA.

• Journal of the Korean Regional Science Association
• /
• v.6 no.2
• /
• pp.107-121
• /
• 1990

The structural change of occupational composition with the coming of post-industrial society is most evidently observed in the developed country. Specially representative of social changes is the emergence of new occupational sectors, such as the so-called quaternary and quinary sectors, which are based on the use of information technology and managerial skills. The paper raises the question of how and in what this chage of occupational components in a mtro area affects the existing residential structure over the period. In order to extend the discussion of the question, the related hypothetical statements on urban spatial impact due to transformation of the society have xtnsively considered and identified a possible multicenter structure concentration and deconcentration of new jobs and population over the metro area. For further examination, the renowned high tech city of Minneaplis -St. Paul has been selected and the occupational labour forces data for 1960 and 1980 analyzed. As a whole, this area has experienced a dispersal and reconcentration of population in new patterns which have resulted from changes in the occupational structure. In particular, the residential area of white-coller workers(the quaternary / quinary or information workers) has expanded into the city from a suburban core area. In this process, a large proportion of the manual-worker's residential areas in the central city either disappeared or diminished. Consequently, other isolated centers created by the manualworker residents are emerging in both the central city and suburbs. Thus, the development of mulitple centers or cores based on the distribution of occupational characteristics can be considered as a typical ongoing pattern of metro areas in the Unite States.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 2006.11a
• /
• pp.3-16
• /
• 2006

In the age where the Internet changes everything, even the earth has become flat. The boarders between nations, locations, times, and industries are not maningful, and no single company can do the whole process well. Therefore, various types of 'Value network' and 'Relation web' emerge for moving first and fast learning. Bort the relationship maturity model (RMM) proposed and the partnership management initiatives at SKT demonstrate that the concept is important, and that the final goal can be reached only through a series of critical outcome at each phase. In particular, recognizing as core infrastructures various online/offline channels, deep trust, and rich communications is an important finding for a successful relationship management. Also, related literatures suggest the following key factors to be influential in more that two phases: professionalism including expertise, similarity, channel capacity. Based on these findings, future efforts need to be put on the research & development of related measurement and management tools. We do hope more people dance with their partners through these efforts.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.48 no.5
• /
• pp.760-767
• /
• 2015

First annulus formation and age determination of otoliths were examined for chub mackerel Scomber japonicus collected in Korean waters over the one year from January to December in 2009. Translucent zone was regarded as an annual mark. Age interpretation criteria was based on the data of the number of translucent zone, capture date, and edge type of the otolith, assuming the nominal birthday to be 1 January. Monthly changes in mean marginal index indicated that translucent zone was formed once a year, mainly in June. The otolith of 0-ring group was detected comparing the progression by month of the smaller fish length, appearing to be a single first opaque zone. The average distance from the core to the first translucent zone was ~1.77 mm, provided as supplementary information to increase ageing accuracy. The ageing criteria for chub mackerel was made to determine correct year-class with the purpose of effective stock assessment. This method using nominal birthdate and edge type analysis could estimate age of fish closer to the true age than purely counting the number of translucent zone on a whole otolith.

• Journal of Korean Society of Rural Planning
• /
• v.23 no.2
• /
• pp.9-17
• /
• 2017

Rural development policy has changed from hardware based development to community revitalization. The purpose of this study is to analyze social network of collaborative activity among rural organizations as fundamental of community. The material used in this study is a record of collaborative activites in the community newsletter of Hong-Dong area. 161 of collaborative activities (links) and 75 of organizations (nodes) are investigated in network. 6 collaborative activity type ('Education', 'Socializing', 'Meeting', 'Culture', 'Event' and 'Labor') is classified. 'Socializing' is inclusive of approximately half of whole network (50.67%). Closeness centraization, degree centralization and betweenness centralization are measured on top in 'Education', 'Meeting' and 'Event' type. Scatter plot analysis using degree and betweenness centrality index, 'Maeul Revitalization Center', 'Balmak Library', 'Woori-Maeul Medical Co-op', 'Support Center for Female Farmers', 'Hongdong Middle School' and 'Mundang Sustainable Agriculture Education Center' are resulted as the core organization in network. Geographical distribution of collaborative activity is not only concentated in Hong-Dong Myeon but also networked with adjacent administrative district. This study finds its purpose in the detailed analysis of network characteristics of collaborative activity within Hong-Dong area which is representative developed rural community in Korea.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.31 no.3
• /
• pp.117-124
• /
• 2008

Ultra-high voltage transformer industry has characteristic of small quantity batch production system by other order processing unlike general mass production systems. In this industry, observance of time deadline is very important in market competitive power security and company continued existence. The transformer winding is a process that rolls a coil is coated with an electric insulation material in order to generate the required voltage using the voltage fluctuation. The winding process is very important production process in the extra-high voltage transformer manufacturing industry because winding process is core process that occupy weight about half of whole process and is process that decide current ratio of transformer. This paper proposes a statistical calculation and control method of planned leadtime on the basis of real data and informations for the A company in transformer winding process. Moreover, we develop unit process scheduling system.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
• /
• 2006.10a
• /
• pp.132-137
• /
• 2006

With the help of the development of digital-multimedia in the middle of 1990's, FDP(Flat Panel Display) had attracted considerable attention. Collimation proximity exposure system that transfers the pattern on wafer or glass exactly using mask and light with appropriate wavelength is core process in semiconductor and liquid display element. The performances of resolution required in precision exposure system are evaluated by resolving power, depth of focus and storage area. Most of development has targeted on these three factors. The optical design including lamp house has played an important role on the performance of exposure process. In this study, we evaluate the cooling system, concerning on exposure device with mercury lamp among the kernel equipment for the production of LCD, to prevent the instability of lighting due to long term accumulation of excessive heating inside the lamp house. Numerical analysis is conducted on full-scale model. The characteristics of three-dimensional flow, pressure and temperature distribution on exposure system are graphically depicted to estimate the whole cooling system for lamp house and to establish the design criteria.

• The Journal of Information Systems
• /
• v.19 no.1
• /
• pp.97-116
• /
• 2010

Failures of super large projects like IT Upgrade of Shinhan Financial Group can be a heavy blow not only to the company but even to the national economy. Research on the practices of risk management in those projects will provide invaluable lessons, enhancing capabilities and chances of successfully executing mission critical projects of the companies and the national economy as a whole. This paper analyzes the risk management of the Core Systems Reconstruction which was the most critical component of IT Upgrade. The analysis covers risk management plans, and identification and evolution, and control and monitoring of risk factors. This study confirms the major results of previous research on risk management in Korea. However, the analysis found as well some discrepancies of practices from the previous research results. This research also tracked the trajectories of evolution of risk factors and management. In particular, in depth analysis of control and monitoring is the first research in Korea on the "management" of risks in IT projects. The result of this research is expected to be a useful guide for theory development and practices of risk management in the future.

• The Journal of Korean Association of Computer Education
• /
• v.18 no.1
• /
• pp.45-54
• /
• 2015

A whole society has become software-driven and each independent fields has become convergence based on computing as the new digital economy era has come. This change affect computational literacy using computational thinking is emphasized the importance as the core competence for the future living. It means that supply of manpower with understanding software principle will decide the fate of the country. For this reason, each country are reinforcing computer science education or computing education. In this paper, we researched and analysed on new global trends of computer education in school especially focused on national curriculum and policy.

• KIEAE Journal
• /
• v.16 no.3
• /
• pp.35-46
• /
• 2016

Purpose: This paper aims to analyse the composition system of architectural elements including shape, kinetic and material elements of kinetic facades and establish the design parameter system as a common conceptual and practical knowledge sharing platform with mechanical and electrical experts. Method: This research has been conducted in a three steps. At first, 120 cases of external shading devices are analyzed and their classification criteria have been established. Secondly geometric, kinetic and material elements are categorized in a common kinetic facade coordinates system considering environmental effects and operation method, and the applicability of combination of each element are tested. Lastly core design parameters for each element have been established in a common office building installation coordinate. Result: Geometry elements are categorized into seven geometric shapes and kinetic elements is categorized into basic linear and rotational motion and combinational folding and rolling motion. The combined set of parameters for three elements composes the whole design parameters for architectural elements of kinetic façade. Design parameters of shape elements are composed of shape, installation and arrangement parameters; design parameters for kinetic elements are composed of axis and range parameters; and design parameters of material elements are composed of thermal, lighting and color parameters.