• Molecules and Cells
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• v.37 no.2
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• pp.149-160
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• 2014

Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

• Weed & Turfgrass Science
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• v.3 no.3
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• pp.225-231
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• 2014

Benzobicyclon induces phytotoxicity to Tongil type rice, although it is an efficient herbicide in paddy. This study was performed to figure out the benzobicyclon-induced phytotoxicity responses of whole crop forage rice genotypes that have the genetic background similar to Tongil type rice, under wet-seeded and transplanted conditions. Mogwoo demonstrated whitening symptom at all the temperatures in both wet-seeding and transplanting by the application of standard and double rates of benzobicyclon. Nokyang, Mogyang and Suweon560 exhibited no or negligible whitening and their plant heights were not reduced at standard rate of benzobicyclon. But in double rate application in wet-seeding, the three genotypes showed 0-8% of white plants at all temperature treatments and reduction in plant height was comparatively larger at $27^{\circ}C$. In transplanting, the double rate application of benzobicyclon at 24 and $27^{\circ}C$ induced 0-32% of whitening plants depending on genotypes while no whitening plants were observed at 18 and $21^{\circ}C$ in all genotypes. Reduction in biomass by double rate of benzobicyclon at 24 and $27^{\circ}C$ was greater than at 18 and $21^{\circ}C$. It is concluded that Mogwoo is extremely sensitive to benzobicyclon and Nokyang, Mogyang and Suweon560 are tolerant to the standard rate of benzobicyclon.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.579-585
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• 2014

This study was conducted to investigate liquid pig manure (LPM) application rates on the growth characteristics, yield, and feed value of whole crop barley in Yeongsangang and Saemangeum reclaimed tidal land. Electronic conductivity (EC), organic matter (OM), and available phosphate (Av. $P_2O_5$) increased in chemical properties of Yeongsangang and Saemangeum soil as raising LPM application level. As increasing LPM application level, exchangeable $Na^+$ significantly increased in Yeongsangang, while exchangeable $K^+$ significantly increased in Saemangeum. Plant height was not significantly different from LPM 100% to LPM 200% in Yeongsangang and in Saemangeum. Dry matter yield of whole crop barley increased steadily, but crop yield of LPM 200% in Yeongsangang ($10.5ton\;ha^{-1}$) was as much as that of LPM 150% ($10.0ton\;ha^{-1}$). Yield of LPM 200% ($11.2ton\;ha^{-1}$) in Saemangeum was similar to that of LPM 150% ($10.5ton\;ha^{-1}$). Crude protein (CP) increased depending on LPM application level, but total digestible nutrients (TDN) increased regardless of LPM application level. LPM 200% was the highest in TDN yield (Yeongsangang: $7.4ton\;ha^{-1}$, Saemangeum: $6.9ton\;ha^{-1}$), but there was no statistical difference between LPM 150% (Yeongsangang: $6.9ton\;ha^{-1}$, Saemangeum: $6.6ton\;ha^{-1}$) and LPM 200%. From the results described above, optimum rate of LPM for cultivating whole crop barley is considered 100% in Yeongsangang reclaimed tidal land and 150% in Saemangeum reclaimed tidal land, showing that the effect of LPM application is better in Segmentation than that in Yeongsangang for yield of whole crop barley.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.2
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• pp.84-88
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• 2001

Two methods of nitrogen application at the 4th leaf stage were evaluated in direct-seeding rice cultivation on dry paddy soil: one was the top dressing of nitrogen on dry paddy condition before irrigation (TNDP) for labor saving, and the other was the top dressing of nitrogen on dry paddy with mechanical rotavation of the inter-row space (TNDPIR) for increasing the efficiency of nitrogen. To evaluate these two application methods, leaf chlorophyll contents, growth characteristics and yields were compared with two conventional methods, basal application (BA) and top dressing on flooded paddy condition at the 4th leaf stage (TNFP). The SPAD value of rice in TNDP was similar with that in TNFP throughout the whole growth stage of rice. Also, there were no differences in rice growth and yield between the two methods. However, in TNDPIR, the SPAD value was the highest during tillering stage among the four application methods, and the tiller number, LAI at heading stage and the panicle number were higher than the two conventional methods.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.235-240
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• 2012

This study was conducted to find out the optimum cropping system for the stable production of forage crops in the newly reclaimed land located at Gwanghwal region of Saemangum reclaimed land in which the soil is sandy loam (Munpo series). There were two treatments of nitrogen fertilization 20% increment based on the standard fertilization of 150, $200kg\;ha^{-1}$. Whole crop barley as the winter crop sowed on 27 October. After the whole crop barley was harvested at the end of May. Corn and sorghum${\times}$sudangrass as the summer crop sowed at the early of June successively on the same field. Emergence rate the whole crop barley was high while the summer crops were low. Soil salinity was increased during cultivation of summer crops. However, corn and sorghum${\times}$sudangrass were not damaged by salt. Increase of nitrogen fertilization made the growth of cultivation crops good, stem and leaf tended to have a lot of the mineral nutrients at heading stage and silking stage. After experiment, among soil chemical properties pH, content of exchangeable sodium were decreased and content of organic matter, available phosphate were increased. Dry matter yield were showed whole crop barley $13,170kg\;ha^{-1}$ and sorghum${\times}$sudangrass $19,440kg\;ha^{-1}$ by increment of nitrogen fertilization. Therefore, to improve the product and nutrient balance of reclaimed saline land comprehensive soil management should be considered.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.160-160
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• 2017

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic Leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of both OsbZIP66 in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.353-360
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• 2011

In order to develop the application method of pig compost (PC) and liquid manure (LM) for whole crop barley cultivation, experiments were conducted at Munpo series (coarse loamy, mixed, nonacid, mesic family of Typic Fluvaquents) soil in Gyehwa-reclaimed land, six plots, a LM applied rate as N% ; non-application, chemical fertilizer (CF)100, 100, 50+50, 50+CF50 and (PC30+LM40)+LM50 as basal and additional fertilizer. $NO_3^-$-N content in soil was decreased as along with the growth of plant, highest in LM100% as basal fertilization at early growth stage and highest in (PC30%+LM40%)+LM40% and CF100% at last growth stage. Amount of $NO_3^-$-N and $NH_4^+$-N in soil was high in (PC30%+LM40%)+LM40% and CF100% of top soil but in subsoil significant difference was little in all treatment. Amount of OM, $A_V.P_2O_5$, T-N, exchangeable Ca and Na in soil was higher (PC30%+LM40%)+LM40% than non-application after harvest. Amount of nutrient uptake in plant was higher in CF100% and split application of LM than LM 100% application. Nitrogen utilization rate was in the order of CF100% >LM50%+LM50%=LM50%+CF50%>(PC30%+LM40%)+LM40% >LM100%. The yield of whole crop barley in (PC30%+LM40%)+LM40% and CF100% was 3.2 times more than in non-application ($309kg\;10a^{-1}$). Feed values such as crude protein and TDN was increased 1.0% ~ 1.4% in LM as split application than basal 100% treatment. Accordingly, in order to increase yield of a whole crop barley with application PC+LM in reclaimed land treat split application rather than to treat LM 100% into the land.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.1
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• pp.48-54
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• 1987

To search for the optimal nitrogen application method for the machine transplanting to Dongjinbyeo, compared to conventional method, in the various methods of nitrogen split application at different growth stages of rice plant. This experiment was performed at Honam Crop Experiment Station, in 1985 year. The results were as followed; the nitrogen contents of leaf blade was the highest at maximum tillering stage under the conventional method and reduction division stage under the nitrogen top-dressing at panicle initiation and 5 times split application of nitrogen for the whole rice growth stages. CGR, RGR and NAR after heading was highest under 5 times split application of nitrogen for the whole rice growth stages. Relationships between CGR and NAR, NAR and RGR, CGR and RGR showed the positive correlation significantly at each rice growth stage, respectively, and showed more high correlation according to further rice growth stages. The degree of contribution of CGR to rice grain yield was the highest at 20 days after heading. Rice grail: yield was higher under top-dressing of nitrogen at panicle initiation and basal application (30%) + 4 times equal dispensing top-dressing after basal application of nitrogen than conventional method.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.519-523
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• 2011

''Nokyang', a new high biomass yield and whole crop silage rice (Oriza sativa L.) cultivar, was developed by the rice breeding team of National Institute of Crop Science, RDA, Suwon, Korea, during the period from 1996 to 2006 and released in 2007. It was derived from a cross between Yongmoonbyeo/IR67396-16-3-3-1. This cultivar has about 130 days of growth duration from seeding to heading and is tolerance to lodging with erect pubescent leaves, semidwarf (culm length 78 cm) and thick culm. This cultivar has less tillers per hill and more spikelet numbers per panicle than Dasanbyeo. 'Nokyang' has wide and stay green leaf compared other Tongil-type varieties. This new variety is resistant to grain shattering and to some disease including bacterial leaf blight and stripe virus. This variety has good qualities for whole crop silage with high TDN (Total Digestive Nutrient) yield and low NDF (Neutral Detergent Fiber) and low ADF (Acid Detergent Fiber) and high RFV (Relative Feed Value) compared to common high grain yield varieties. The biomass and TDN yield performance of 'Nokyang' is 1,652 MT/ha, 9.9 MT/ha, individually in local adaptability test for three years. 'Nokyang' is adaptable to central plain area, south-eastern plain area of Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.1-8
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• 2007

This experiment was conducted to determine the effect of growth stage and variety on the yield and quality of whole crop rice (WCR) at National Livestock Research Institute, RDA from 2003 to 2005. Two types of rice ("Chucheong" for food and "Hamasari" for feed) were harvested at six different growth stages (heading, flowering, milk, dough, yellow ripening and fully ripening stage). Crude protein content decreased with progressed maturity at harvest. As growth stage delayed, TDN (total digestible nutrient) content of all rice varieties increased. The average TDN content of two type of rice was about 60% (59.3) which was calculated from ADF(acid detergent fiber) content. The contents of ADF and NDF(neutral detergent fiber) decreased with harvest maturity. The highest DM(dry matter) yield was at the ripening stage (p<0.05) and "Chucheong" gave higher yields than for "Hamasari". In this experiment, the DM yield of all varieties was low. The yield of TDN followed a similar trend to DM yield. According to this results that dough to yellow ripening stage of harvest maturity will be recommendable as proper harvest time for whole crop rice silage.