• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.341-352
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• 2021

Acerola is an excellent ingredient because of its high natural vitamin C content, but it is difficult to stabilize and has hardly been studied as a cosmetic material. Therefore, this study developed a mixed liposome preparation for stabilizing acerola extract. As a safety test, the skin irritation test was evaluated by BCOP assay and HET-CAM assay. We evaluated the inhibition of tyrosinase activity, the whitening effect of melanin production, and the wrinkle effect of prochloragentype-I C-peptide production, and confirmed the possibility of functional cosmetics. In addition, a cream of liposomes containing acerola extract mixture was developed to evaluate the clinical studies of skin wrinkles and whitening. BCOP assay, HET-CAM assay and human skin primary irritation test results of liposomes containing acerola extract mixture showed no irritation and were safe from skin and eye. The result of tyrosinase activity by 75.8% at 1,000 ㎍/mL. As a result of the melanogenesis inhibition test, liposome with acerola extract showed the melanin content by 46.2% at 1,000 ㎍/mL that does not effect the viability of the B16F10 cell line. The result of collagen production test using ELISA kit, liposomes containing acerola extract mixture showed collagen synthesis ability by 152.1% at 1,000 ㎍/mL that does not affect the viability of the HS68 cell line. But it did not showed any inhibition of collagenase (MMP-1) activity at all concentrations in the MMP-1 activity inhibition test in the HS68 cell line. We performed clinical studies for the whitening and skin-wrinkle activity of cream containing acerola extract mixes liposome, was showed that the melanin contents and wrinkle was statistically significant reduction. These results suggest that liposomes containing acerola extract mixture have safe natural material, and skin wrinkle, whitening effects allowing their application in cosmetics as a natural product.

• Food Science and Preservation
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• v.22 no.2
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• pp.261-266
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• 2015

In this study, we investigated the applicability of functional materials by examining various physiological activities with an extract from the Agrimonia pilosa root. The A. pilosa extract showed low cytotoxicity against murine melanoma B16F10 cells. With little or no cytotoxicity at various concentrations, the A. pilosa extract showed high levels of DPPH radical scavenging activity ($ID_{50}$, 20.70 mg/L) and anti-microbial activity against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, it had a high level of anti-microbial activities against Gram-positive bacteria. These results suggest that the A. pilosa extract can be used as a natural preservative. It also showed inhibition of tyrosinase activity ($ID_{50}$, 90.18 mg/L), as does kojic acid ($ID_{50}$, 89.13 mg/L), and especially, a higher decrease in melanin content ($ID_{50}$, 62.5 mg/L) than the arbutin level ($ID_{50}$, 100.7 mg/L) as a positive control. These findings suggest that the A. pilosa extract inhibits melanin synthesis by suppressing the intracellular tyrosinase expression. These results indicate that the A. pilosa extract may be an effective material for functional cosmetics, such as skin whitening materials.

• Journal of the Korean Applied Science and Technology
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• v.33 no.4
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• pp.706-716
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• 2016

In this study we applied the NAG obtained by the deacetylation of chitin extracted from the shells of crabs and shrimp as cosmetic ingredient. In order to compare NAG with GLC we identified the influence of cytotoxicity, anti-inflammatory, melanin biosynthesis formation inhibition on skin cell, and we measured the effects of the change of melanin and red spots. The results show that there was not any attentive cytotoxicity on the Raw 264.7 cell and B16F10 cell, and NO formation anti-inflammatory hindrance effect induced from Raw 264.7 by LPS was slight, and NAG suppressed the increase of melanin generation concentration-dependently after we induced the melanin generation with ${\alpha}$-MSH on B16F10 and measured the melanin biosynthesis inhibition. From this result, we identified the applicability of the cosmetics containing NAG as functional cosmetic for enhancing skin-lightening because when cream containing NAG was applied to skin the index of melanin red spots showed statistically meaningful changes.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.65-71
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• 2005

A silsesquioxane precursor that contains p-methoxycinnamoyl group was synthesized to produce UV absorbing spherical polysilsesquioxane (PSQ) spheres with diameters of 10nm to a few ${\mu}m$ under different conditions. A dispersion of $10wt\%$ in Vaseline of these PSQ spheres with diameters of approximately $0.6{\mu}m$ showed SPF values of 5.7 and the identical dispersion did not turn white when applied to human hands. These UV absorbing PSQ spheres showed higher boosting effect of sun protection factor (SPF) than the well known UV blocking octyl methoxycinnamate (OMC). The results indicated that the PSQ spheres with p-methoxycinnamoyl group should be useful as the ingredients in UV screening functional cosmetics without causing allergies and whitening.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.153-157
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• 2015

In this study, we investigated the applicability of functional materials by examining a variety of physiological activities with the extract of Cleyera japonica leaf. Cleyera japonica extract showed a low cytotoxicity against murine melanoma B16F10 cells. In little or no cytotoxicity at concentrations, we showed that the treatment with Cleyera japonica extract resulted in a significant increase in the DPPH radical scavenging activity (IC₅₀, 22.90 ㎎/L), similar to ascorbic acid (IC₅₀, 18.65 ㎎/L) and anti-microbial activities against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, anti-microbial activities against Gram-positive bacteria was high. These results suggest that Cleyera japonica extract could be used as a natural preservative. Additionally, Cleyera japonica extract showed the inhibition of tyrosinase activity (IC₅₀, 178.90 ㎎/L), similar to kojic acid (IC₅₀, 89.13 ㎎/L) and decreased melanin content (IC₅₀, 101.90 ㎎/L) higher than the control arbutin level (IC₅₀, 100.65 ㎎/L), especially. Therefore, these results indicate that Cleyera japonica extract may be an effective material for functional cosmetics such as skin whitening materials.

• Journal of Convergence for Information Technology
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• v.11 no.8
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• pp.212-223
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• 2021

This study has purposes : evaluating how safe the skin is when applied with the Spicules-containing cosmetic, and whether Diamond-spicules is permeated into the skin and showed the efficacy of the active ingredients well. For these aims, women in their 30s and 60s were surveyed for skin irritation and changes after applying cosmetics to their skin for four weeks. As a result, Spicules-containing cosmetics were safer on the skin than general functional cosmetics, and in particular, in the case of cosmetics containing Diamond-spicules, statistically significant results (p<.001) were obtained in improvement of anti-wrinkle, firmness, moisturizing, whitening, alleviation of inflammation, and sensitivity. Thus, It has been proved Diamond-spicules containing functional active ingredients, not only as transdermal delivery system, but also can be used as multifunctional cosmetic material.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.313-313
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• 2022

The Smilacis chinae Radix refers to the root of Smilax chinae L distributed in mountain and filed of Korea, and it is a vine shrub in the Lilaceae family, called Berchemia berchemiaefolia, and is referred to as Smilacis chinae Radix in it's a natural medicine name. Antibacterial, inflammatory, and antioxidant activity were studied in Smilacis chinae Radix. In this study, biological activities such as antioxidant (DPPH, ABTs, TPC), cytotoxicity, wrinkle improvement, and whitening improvement to increase the utilization value of Smilacis chinae Radix and identify the botanical value. Therefore, we tried to explore the applicability of Smilacis chinae Radix as a functional cosmetic material. Smilacis chinae Radix (SCR) was dried and extracted with ethanol. In order to measure the biological activity of the SCR, antioxidant activity, inhibition activities of collagenase, tyrosinase and cell viability were measured. The DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity in the extract with a concentration of 400㎍/mL is 91.22% ± 0.41%%. ABTs (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity in the extract with a concentration of 400㎍/mL is 99.60% ± 0.03%. Total polyphenol contents (TPC) are 0.203 ± 0.05 mg GAE/mg Ext when SCR was lmg/mL. And the Cell viability for HaCaT derived human keratinocyte and Raw264.7, a mouse-derived macrophage was determined using the MTT assay. When cell was treated with 100㎍/mL of SCR, HaCaT cell showed cell viability of 78.09 ± 0.1% and Raw264.7 cell showed cell viability of 91.88 ± 0.42%. From the above results, we have shown the possibility that the CSR have antioxidant ability, inhibition activity of collagenase and tyrosinase and cell safety ability which can be useful in a functional cosmetic material.

• Journal of the Korean Home Economics Association
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• v.44 no.11
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• pp.43-50
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• 2006

The purpose of this study is to analyze the use and satisfaction on cosmetics of middle- and old-aged women with well-being pursuit disposition. A survey was conducted from January 20 to February 10 in 2006. The statistical analysis methods used to analyze the 393 middle- and old- aged female subjects were frequency analysis, reliability analysis, factor analysis, cluster analysis, crosstab, and multiple regression analysis. First, middle- and old-aged women were using functional cosmetics for wrinkle and whitening as well as skin lotion, moisture lotion and nutritive cream. They applied basic skin make-up only or partial make-up on the eyes and lips. Second, the well-being pursuit disposition factors were determined to be eco-friendly pursuit disposition, economical pursuit disposition, and health pursuit disposition. The well-being pursuit disposition factors had an effect on satisfaction of basic cosmetics. While the economical pursuit disposition factor had an effect on satisfaction of colored cosmetics. Third, the clusters from well-being pursuit disposition were determined to be wellbeing unconcerned group, eco-friendly concerned group, economical concerned group, and health concerned group. The proportion of subjects applying basic and colored cosmetics was high in health concerned group.

• Journal of Applied Biological Chemistry
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• v.51 no.1
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• pp.7-12
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• 2008

The suitability of CPWs, by-products of the juice industry, was investigated as a source for the production of cosmeceuticals. Four kinds of CPWs, CW, CWE, CWER, and CWEA, were examined for their antioxidant potentials in terms of DPPH radical-scavenging ability for anti-wrinkle applications, inhibition of tyrosinase or melanin production for whitening products, and anti-inflammatory effects to treat various skin diseases such as atopic dermatitis and acne as well as for anti-bacterial activity against acne-inducing pathogens. Of the four extracts, CWER was the most potent tyrosinase inhibitor ($IC_{50}$ value: $109\;{\mu}g/mL$), and CWEA ($IC_{50}:\;167\;{\mu}g/mL$) showed good antioxidative effects. CWE and CWEA samples had dose-dependent inhibitory effects on the melanin production. The cytotoxic effects of the four CPWs were determined by colorimetric MTT assays using human keratinocyte HaCaT cells. Most extracts exhibited low cytotoxicity at $100\;{\mu}g/mL$. These results suggest CPWs are attractive candidates for topical applications on the human skin.

• Food Science and Biotechnology
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• v.15 no.2
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• pp.317-320
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• 2006

Octadeca-9,12-dienyl-3,4,5-hydroxybenzoate (GA-LA) was chemically synthesized from gallic acid and linoleic acid ester, and its biological functions were tested. Radical-scavenging activity of GA-LA was comparable to those of gallic and ascorbic acids at 0.24 mM, and tyrosinase inhibition effect was higher than that of ascorbic acid. Gallic and linoleic acids did not show any tyrosinase activity. Results of cyclooxygenase (COX) inhibition effect indicate GA-LA has higher selectivity in COX-1 inhibition. GA-LA from gallic and linoleic acids could be used as functional reagent for antioxidative, skin-whitening, and anti-inflammatory effects in food, pharmaceutrical, and cosmetic industries.