• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.223-229
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• 2013

This study aimed to explore the clinical, radiological, and pathological characteristics of intraocular cysticercosis due to Taenia solium metacestode infection. Total 8 patients diagnosed with intraocular cysticercosis at the Red Cross Hospital of Yunnan Province, China were examined retrospectively. Patients with clear dioptic media had undergone fundus chromophotography. All patients underwent B ultrasonography of the ocular region (CT) successive scanning of the orbit and cerebral tissues. Parasites were extracted surgically and then examined pathologically. The fundus chromophotography showed a white and condensing scolex package in the vesicle. The B ultrasonic examination showed a vesicle-like echogenic mass in the vitreous chamber, in which the high-level echo spot was the cysticercus scolex. The pathological examinations showed that the vesicle wall exhibited hyaline degeneration, inflammatory cell infiltration, neuroglial fiber, and glial cell proliferation layers from the inside to the outside. The scolex is round and is composed of the outer tissue (the body wall) and the inner furrow tissue; these tissues migrated together. Primordially differentiated sucking discs were found in one case, but no hooklets were found. The inner scolex tissue was folded like a paper flower. The severity of intraocular disease is closely correlated with the pathophysiological processes of the cysticercus worm. Pathological examination of the intraocular lesions can help to evaluate the course of the disease as well as to provide a scientific basis for effective antiparasitic medication.

• 한국식물병리학회지
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• 제2권1호
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• pp.48-52
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• 1986

흰토끼풀에 황색모자이크병징을 나타내는 이병주를 채집하여 병즙액의 지표식물반응과 물리적 성질조사, 풍매전염시험, 바이러스입자의 형태관찰 그리고 항혈청반응 등의 방법으로 병원바이러스의 동정을 시도한 결과 강낭콩황반모자이크바이러스(BYMV)로 밝혀졌다. 공시병원바이러스의 지표식물반응은 명아주에 전체국부반응, 잠두의 접종엽에 국부반응, 줄기와 상위엽에 각각 양저증상과 모자이크반응을 보였으며, 강남콩에는 엽맥양저, 황색모자이크 또는 축엽이 되고 순이 고사하였다. 또한 완두, 녹두, 팥과 red clover에는 모자이크 반응을 나타냈다. 바이러스입자의 형태 및 크기는 740nm정도의 사상입자이며, 복숭아혹진딧물에 의하여 전염이 가능하였다. 바이러스병즙액의 생리적 성질은 내열성이 $60\~65^{\circ}C$, 내희석성이 $10^{-3}\;-\;10^{-4}$, 내보존성이 3일이었다. 순화된 공시바이러스는 일본 식물바이러스 연구소로부터 분양받은 BYMV항혈청과의 혼합침강반응결과 양성반응을 보였다.

• 식물병연구
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• 제26권1호
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• pp.53-56
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• 2020

2019년 5월, 잎 표면에서 적색 반점이 관찰되는 참소리쟁이를 충북 괴산군 인근 야산에서 채집하였다. 관찰된 병반의 앞면은 적색 및 황색의 병반이 관찰되었고, 뒷면에는 백색의 돌기가 관찰되었다. 다양한 현미경으로 자세하게 관찰한 결과, 돌기는 녹포자기로 확인되었으며, 많은 녹포자가 확인되었다. 녹포자는 구형 또는 난형이었고 19.0-24.4×19.0-24.5 ㎛의 크기를 가지면서 표면은 무사마귀형으로 관찰되었다. 계통학적 유연관계 분석을 위하여, large subunit rDNA 영역 및 cytochrome c oxidase subunit 3 (CO3) 유전자 영역을 이용하여 계통학적 유연관계를 분석한 결과, 참소리쟁이에서 관찰된 녹병균은 Puccinia pritzliana와 매우 근연한 관계를 가지고 있었다. 관찰된 기주, 포자세대, 형태 및 계통학적 유연관계 결과를 종합해 볼 때, 분석된 녹병균은 국내 미보고 녹병균인 P. phragmitis로 확인하였으며, 위 병원균에 의해 발생한 녹병을 참소리쟁이 잎녹병으로 제안하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1184-1189
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• 2012

White spot syndrome virus (WSSV) is one of the major viral pathogens affecting shrimp aquaculture. Four proteins, WSSV199, WSSV 222, WSSV 249 and WSSV 403, from WSSV are predicted to encode a RING-H2 domain, which in presence of ubiquitin conjugating enzyme (E2) in shrimp can function as viral E3 ligase and modulate the host ubiquitin proteasome pathway. Modulation of host ubiquitin proteasome pathway by viral proteins is implicated in viral pathogenesis. In the present study, a time course expression profile analysis of WSSV Open Reading Frame (ORF) 199 and Penaeus monodon ubiquitin conjugating enzyme (PmUbc) was carried out at 0, 3, 6, 12, 24, 48 and 72 h post WSSV challenge by semi-quantitative RT-PCR as well as Real Time PCR. EF1${\alpha}$ was used as reference control to normalize the expression levels. A significant increase in PmUbc expression at 24 h post infection (h.p.i) was observed followed by a decline till 72 h.p.i. Expression of WSSV199 was observed at 24 h.p.i in WSSV infected P. monodon. Since the up-regulation of PmUbc was observed at 24 h.p.i where WSSV199 expression was detected, it can be speculated that these proteins might interact with host ubiquitination pathway for viral pathogenesis. However, further studies need to be carried out to unfold the molecular mechanism of interaction between host and virus to devise efficient control strategies for this chaos in the shrimp culture industry.

• 한국수산과학회지
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• 제51권3호
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• pp.248-253
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• 2018

Freshwater farms are primarily located adjacent to rivers and lakes, facilitating the introduction and spread of pathogens into natural systems. Therefore, it is necessary to continuously monitor natural aquatic organisms, the breeding environment, and infection rates by pathogenic organisms. Fish and crustaceans were sampled 4 times in the Geum River estuary in 2016. The samples were analyzed for the presence of pathogens for reportable communicable diseases, including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease); parasite abundance was also examined. The dominant fish species were deep body bitterling Acanthorhodes macropterus (21.4%), followed by skygager Erythroculter erythropterus (12.7%). For crustaceans, Palaemon paucidens and Chinese mitten crab Eriocheir sinensis were dominant. Sixty fish and 36 crustacean species were examined for reportable communicable diseases. When using a specific primer set for each disease, PCR analysis did not detect any reportable communicable diseases in the samples. Some instances of Dactylogyrus, copepods, nematodes and metacercaria were detected. However, the PCR results indicated that the metacercaria were not Clonorchis sinensis.

• 한국수산과학회지
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• 제51권1호
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• pp.47-53
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• 2018

The presence of pathogens in wild fish is a potential threat to the fish being raised nearby fish farm. Surveillance of these pathogens in the wild is, thus, highly important to keep fish in the farm safe from serious communicable diseases. Fish and crustacean were sampled 4 times at Soyang Lake in 2016. Pathogens for five reportable communicable diseases including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease), and parasites were investigated. In Soyang Lake, pale chub Zacco platypus was the most abundant. Forty seven pale chub, 8 Korea piscivorous chub Opsarichthys uncirostris, 3 oily shiner Sarcocheilichthys variegatus wakiyae and 2 crucian carp Cyprinus carassius were investigated for detection of target diseases of fish. Seventeen Caridina denticulata denticulata, 12 Oriental river prawn Macrobrachium nipponense and 1 Chinese mitten crab Eriocheir sinensis were used for detection of WSD. As the result, reportable communicable diseases were not detected in all the freshwater animals. Dactylogyrus was detected in Korea piscivorous chub. Copepoda was also detected in pale chub and Korea piscivorous chub. Metacercaria was detected in the pale chub sampled in September and October. Those were not metacercaria of liver fluke Clonorchis sinensis.

• 대한공간정보학회지
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• 제21권3호
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• pp.55-62
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• 2013

본 연구에서는 전방위 카메라(Omni-directional Camera)를 이용하여 과속방지턱(Speed Bump)을 탐지하고 Vision Based Approach 통한 실시간 과속 방지턱 데이터의 갱신 방안을 제시하는 것을 목적으로 한다. 카메라 영상정보에서 과속 방지턱을 검출하기 위해 잡음을 제거하고 이를 구성하는 형상과 패턴으로 여겨지는 점들을 우선적으로 탐지하여야 한다. 과속방지턱은 일정한 폭과 규칙적인 형태를 유지하며 흰색과 노란색의 영역을 가지고 있음에 착안하여 침식과 팽창을 이용한 형태학적 연산과 HSV칼라 모델을 적용하여 도로상의 과속방지턱을 추출하였다. 카메라에서 거대한 이미지 데이터를 수집하여 대상 객체를 검출하고 GPS 위치 정보를 이용하였다. 마지막으로 동시적 위치추정 및 지도작성 (SLAMs :Simultaneous Localization And Mapping) 시스템을 구현하여 탐지알고리즘과 취득결과의 정확성을 평가하였다.

• 한국동물위생학회지
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• 제33권1호
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• pp.67-74
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• 2010

To reduce an economic loss by swine disease, this study was designed to provide helpful data which are particularly useful for each individual farm. From february to December 2009, a total of 18,173 slaughter pigs (612 herds) were randomly sampled, which were produced just in southern region of Gyeonggi-do. We examined all of them for the slaughter lesions of 8 kind diseases such as swine enzootic pneumonia (SEP), pleuritis (PL), pleuropneumonia (PP), white milk spot in liver (WMS), papular dermitis (PD), pericarditis (PC), ileitis (IL) and peritonitis (PT). Twenty four percentages (4431/18173 pigs) of the examined pigs had no lesions about 8 kind diseases. Among the pigs with lesions, the numbers of the pigs with just one lesion were 7,637(42%), followed by 4,551(25%) pigs with 2 lesions. Average prevalence of pigs were 56.5% (10288/18173 pigs) in SEP, followed by 34% in PL, 12.4% in PP, 10.1% in WMS, 6.1% in PD, 4.7% in PC, 0.1% in IL and PT, respectively. Each prevalence of SEP, PL, PP and PD was higher in spring than in winter, respectively (P<0.01). Among the pigs (n=6,105) with 2 or more than 2 kinds of lesions the top (55.5%) was the pigs with SEP and PL, and the second was 1,179 (19.3%). Swine enzootic pneumonia was considered as one of the more likely risk factors for initiation or/and acceleration of other diseases such as PL, PP, WMS and PD. The lesion of SEP was relatively severe since the pigs with late stage were more (7,277 pigs) than those with early stage.

• 한국실내디자인학회논문집
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• 제21권5호
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• pp.264-271
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• 2012

As changed population structure variously, population decrease is one of the important problem in present urbanization. It resulted decline of the medium-large size housings that increasing of 1-2 person household need the variety of small housing with housing type. This study was researched the Gold-Generation on theme by Gold Miss, Gold Mr. who preferring urban small housings that it analyzed survey of Gold generation who be wanted urban small housing by interior coordination elements. Data to be analyzed that first, Gold Mr. and Miss are preferred two bedroom applied by spatial structure coordination. Especially it is based on the separation between livingroom and bedroom with duplex type which is bathroom formed single unit type(shower/toilet/basin). In interior coordination of spatial elements, Gold Mr. considered the form of kitchen with huge ㄷ type instead of Gold Miss are preferred alpha room or alpha space to use powder room or dress room. Second, In Preference of interior coordination elements, Gold Mr. and Miss are preferred bed-clothes and curtains by fabrics, lighting are hanging and spot lighting, accessories are carpet with rug and porcelains. Color are preferred bright scheme both white and ivory, however preference of furniture is different from Gold Mr. and Gold Miss that Gold Mr. are ordered desk, sofa and shelves but Gold Miss are preferred sofa, bed, and dressing table. It showed between Gold Mr. and Gold Miss are equal needs or differences. It expects the basic research for understanding the interior coordination elements for preference in urban small housing as focused on Gold generation(Gold Miss, Gold Mr.) that they will be applied the interior space on urban small housing.

• 한국동물위생학회지
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• 제39권4호
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• pp.231-237
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• 2016

The present study was conducted to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. During March to December 2015, a total of 1,160 lung samples out of 58 herds were collected randomly from pigs slaughtered in Jeonbuk province. In addition, 290 hilar lymph nodes from pig with pneumonic lung lesion (5 samples per herd) were screened for selected viral and bacterial pathogens. Gross lesions of lungs such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis and liver white spots were examined. The overall prevalence of SEP was 64.3% (746/1,160). In the analysis of seasonal prevalence, there was an increase of occurrence during the spring months (287/400, 71.8%) and decrease during the fall months (93/200, 46.5%) among the whole herds. The mean number of SEP score per pig was $1.20{\pm}1.28$. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 25.5% (296/1,160), 44.1% (512/1,160), 3.8% (44/1,160) and 17.6% (204/1,160), respectively. The most frequent region with lung lesion was diaphragmatic lobes (left 17.1%, right 17.3%). In the detection of viral pathogens by PCR, porcine circovirus type2 (PCV2) was positive in 86.9% (252/290), while porcine reproductive and respiratory syndrome virus (PRRSV) was not detected, In the case of bacterial pathogens, 50 microorganisms were isolated by PCR and/or microbiological test. The most frequently isolated bacteria was Streptococcus suis (20, 34.4%), followed by Pasteurella multocida (17, 29.3%), Streptococcus spp. (11, 3.4%), Actinobacillus pleuropneumoniae (2, 8.9%).