• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1257-1261
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• 2006

Pleiomorphic adenoma gene-like1 (PLAGL1) encodes a zinc-finger (ZF) protein with seven ZFs of the C2H2-type which is a regulator of apoptosis and cell cycle arrest, and also regulates the secretion of insulin. In both human and mouse, PLAGL1 is a candidate gene for tumor suppressor and transient neonatal diabetes mellitus (TNDM). In this study, a 2,238 bp fragment covering the complete coding region was obtained and deposited to GenBank (accession number: DQ288899). The reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that PLAGL1 was expressed almost equally in heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus and ovary. Comparing the sequences of Large White and Meishan pigs, a C-T transition in exon 6 was found. The polymorphism could be detected by TaqI and was genotyped in five purebreds (Large White, Landrace, Meishan, Tongcheng and Bamei). Association analysis was performed between the polymorphism and carcass traits in 276 pigs of a "Large White${\times}$Meishan" F2 resource population. As a consequence, significant associations of the genotypes with shoulder backfat thickness (SFT) and internal fat rate (IFR) were observed. Pigs with TT genotype had low SFT and high IFR compared with TC or CC genotypes.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1485-1489
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• 2007

Achaete-scute like 2 (ASCL2) gene encodes a member of the basic helix-loop-helix transcription factor which is essential for the maintenance of proliferating trophoblasts during placental development. ASCL2 gene preferentially expresses the maternal allele in the mouse. However, it escapes genomic imprinting in the human. In this study, the complete open reading frame consisting of 193 amino acids of ASCL2 gene was obtained. Sequence analysis indicated that a C-G mutation existed in the 3' region between Meishan and Large White pigs. The polymorphism was used to determine the monoallelic or biallelic expression with RT-PCR-RFLP in pigs of Large $White{\times}Meishan$ $F_1$ hybrids. Imprinting analysis indicated that the ASCL2 gene expression was biallelic in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). PCR-RFLP was used to detect the polymorphism in 270 pigs of the "$Large\;White{\times}Meishan$" $F_2$ resource population. The statistical results showed highly significant associations of the genotypes and fat meat percentage (FMP), lean meat percentage (LMP) and ratio of lean to fat (RLF) (p<0.01), and significant associations of the genotypes and loin eye area (LEA) and internal fat rate (IFR) (p<0.05).

• The Journal of Korean Physical Therapy
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• 제22권3호
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• pp.17-21
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• 2010

Purpose: This study was designed to investigate possibilities for quantitative analysis using the electromyography and sonography. For better understanding, we evaluated the correlation between the pressure pain threshold and sonography, spontaneous electrical activity in trigger points located in the upper trapezius muscle. Methods: Thirty three active subjects volunteered to participate in this study (n=33). They had a palpable taut band, exquisite spot tenderness of a nodule in a taut band, spontaneous pain, referred pain, jump sign, local twitch response, and a painful limit to full stretch range of motion. We measured Pressure pain threshold, density, white area index, root mean square, and reaction. Pearson’s correlation coefficient was calculated to estimate the relationship between the pressure pain threshold and other variables including density, white area index, root mean square, and reaction time. Results: There were significant correlations between pressure pain threshold and density (r=-0.75, p<0.01), and between pressure pain threshold and white area index (r=-0.74, p<0.01). A significant correlations between pressure pain threshold and root mean square (r=-0.59, p<0.01). The significant correlation was found between pressure pain threshold and reaction time (r=-0.64, p<0.01). Conclusion: These should indicate whether quantitative analysis can be done using the characteristics of electromyography and sonography.

• Journal of Periodontal and Implant Science
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• 제28권4호
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• pp.559-568
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• 1998

direct bone apposition during bone remodelling. To address these problem, we developed a new ceramic, calcium metaphosphate(CMP), and report herein the biologic response to CMP in subcutaneous tissue, muscle and bone. Porous CMP blocks were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. Macroporous scaffolds were made using a polyurethane sponge method. CMP block possesses a macroporous structure with approximate pore size range of 0.3-1mm. CMP blocks were implanted in 8mm sized calvarial defect, subcutaneous tissue and muscle of 6 Newzealand White rabbits and histologic observation were performed at 4 and 6 weeks later. CMP blocks in subcutaneous tissue and muscle were well adapted without any adverse tissue reaction and resorbed slowly and spontaneously. Histologic observation of calvarial defect at 4 and 6 weeks revealed that CMP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CMP blocks didn't show any adverse tissue reaction and resorbed spontaneously also in calvarial defect. This result revealed that CMP had a high affinity for bone and was very biocompatible. From this preliminary result, it was suggested that CMP was a promising ceramic as a bone substitute and tissue engineering scaffold for bone formation.

• 대한치과보철학회지
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• 제36권3호
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• pp.427-452
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• 1998

To elucidate the effects of food texture such as hardness, cohesiveness, adhesiveness on mastication procedure, the electromyographic activity (EMG) of the masticatory muscles and the chewing movements were analyzed in eighteen young subjects during ordinary chewing. Seven different foods were selected by sensory texture profiling and mechanical test with texturometer; egg white, sausage, pizza cheese, yang-geng, biscuit, peanut and almond. The indexes of jaw movements used were the chewing number, chewing time, maximum opening, average opening, closing velocity and opening velocity. The EMG indexes analyzed were maximum and average integrated amplitude of the anterior temporal and masseter muscles. The results obtained were as follows. 1. The chewing time and chewing number, mandibular displacement and mandibular movement velocity were not changed by the hardness, cohesiveness and adhesiveness of the food (p>0.05). 2. The harder food materials showed a higher amplitude of the anterior temporal muscle integrated EMGs than the softer ones (p<0.05). 3. The maximum and average integrated EMGs of the masseter muscle increased with the increase of hardness of the food (p<0.05). 4. The integrated EMGs of preferred side and non-preferred side masticatory muscles increased with the increase of hardness of the food (p<0.05). 5. The adhesiveness and cohesiveness of the food were not the determining factor to the integrated EMGs of masticatory muscles (p<0.05).

• 대한의용생체공학회:의공학회지
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• 제30권2호
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• pp.179-184
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• 2009

Magnetic Resonance Electrical Impedance Tomography (MREIT) is a new bio-imaging modality providing cross-sectional conductivity images from measurements of internal magnetic flux densities produced by externally injected currents. Recent MREIT studies demonstrated successful conductivity image reconstructions of postmortem and in vivo canine brain. However, the whole head imaging was not achieved due to technical issues related with electrodes and noise in measured magnetic flux density data. In this study, we used a new carbon-hydrogel electrode with a large contact area and injected 30 mA imaging current through a canine head. Using a 3T MREIT system, we performed a postmortem canine experiment and produced high-resolution conductivity images of the entire canine head. Collecting magnetic flux density data inside the head subject to multiple injection currents, we reconstructed cross-sectional conductivity images using the harmonic $B_z$ algorithm. The conductivity images of the canine head show a good contrast not only inside the brain region including white and gray matter but also outside the brain region including the skull, temporalis muscle, mandible, lingualis proprius muscle, and masseter muscle.

• Journal of Chest Surgery
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• 제25권6호
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• pp.598-604
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• 1992

Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood and acounts for 6% to 15% of all cases of childhood cancer, Rhabdomyosarcoma in seventh most common form of childhood neoplasms, following acute leukemia, tumors of the central nervous system, lymphoma neuroblastoma, Wilm`s tumor, bone tumor. Rhabdomyosarcoma can arise anywhere in the body, but primary site in the thorax is relatively rare. We experienced a case of aveolar rhabdomyosarcoma arising from intercostal muscle, A 12 year-old woman was suffered from the intermittent left chest pain radiating to the scapular area and dyspnea, On physical examination, pulmonary friction rub was heard on the left upper lobe area. Qn adimission, the chest simple radiography revealed a 7 x 6, 5cm sized radio-opaque mass with pleural effusion in the superior mediastinum and the CT showed a well difined radio-opaque mass including the destructed 2nd rib and pleural effusion. The percutaneous tra-nsthoracic needle aspiration biopsy was likely to show blastoma. After the chemotherapy[vincristine, actinomycin-D, cyclophosphamde] was done to treat blastoma, the pleural effussion was subsided and the mass was slightly decreased by 4.5x 4. 5cm. For treatment and diagnosis, we performed en-bloc resection and the defected chest was reconstucted with Gortex patch. Grossly, the specimen was colored graysh-white and arised in between two ribs The microscopic findings showed that the tumor cells were small round with scant pinkish cytoplasm on the H-E stain and the tumor cell nests were grouped by reticulum fibers and showed alveolar pattern on the silver stain The electromicroscopic finding presented that the cytoplasm contained tangled fibrillar and flocculent materials. The histopathologic findings were compatable with laveolar rhabdomyosarcoma. She was discharged without any complication. After discharge, she has been treated with radiation theraphy and chemotheraphy, and not recurred untill last follow-up We report a case of alveolar rhabdomyosarcoma arising to intercostal muscle, developed in 12 year-old waman, with brief review of literatures.

• 한국식품영양과학회지
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• 제17권1호
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• pp.33-41
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• 1988

12종(種)의 활어(活魚) 또는 신선어(新鮮魚)의 근육(筋肉)에 있어서 K치(値)의 증가율(增加率) 및 IMP 분해효소(分解酪素)의 활성(活性)에 관(關)한 실험결과(實驗結果)는 다음과 같다. 1. K값은 붉은살어(魚)보다는 흰살어(魚)의 선도판정(鮮度判定) 지표(指標)로서 적당(適當)하나 적신어(赤身魚)라도 혈합육(血合肉)의 함량(含量)이 $0.5{\sim}31%$이므로 지장(支障)이 없을 것으로 사료(思料)된다. 2. K값의 변화속도(變化速度)와 IMP 분해효소(分解酪素) 활성간(活性間)의 상관계수(相關係數)는 0.77이며 고도(高度)의 유의성(有意性)이 인정(認定)된다. 3. 어육내(魚肉內)에는 IMP 분해효소(分解酪素) 활성(活性)의 저해제(沮害劑)가 내재(內在)할 것으로 사료(思料)된다 4. IMP에 대(對)한 PH 활성곡선(活性曲線)은 어종(魚種)에 따라 다른데, 이것은 5'-N tase나 P Mase의 양적(量的), 질적(質的), 존재양식(存在樣式)이 어종(魚種)에 따라 다르기 때문이라 생각된다.

• 한국식품영양과학회지
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• 제32권3호
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• pp.400-405
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• 2003

산과 알칼리 수리미 제조공정을 확립하기 위하여 단백질 가용화와 회수를 위한 최적 pH, 이온강도의 영향, 균질화 조건, 마쇄육에 대한 수도수의 비 및 수율을 검토하고, 산 및 알칼리 수리미 공정에 따른 폐수의 오염 저감 효과를 수세수리미와 비교하였다. 어육 단백질을 추출하기 위한 최적 pH는 2.5와 10.5부근이었으며, 가용성 단백질의 침전 회수를 위한 최적 pH는 5.0 부근이었다. 추출 용액의 이온강도 증가는 어육 단백질의 추출량을 현저히 감소시키는 것으로 나타났고, 균질화를 위한 최적 조건은 9500 rpm 이하에서 30초였다. 원심분리 부하량과 파괴강도 및 변형값을 고려한 최적수량은 어육에 대하여 6배량이었다. 산 혹은 알칼리 공정의 수리미 수율은 수세 공정에 비하여 높았으며, 폐수의 오염 부하량은 현저히 낮은 것으로 나타났다.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1032-1038
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• 2003

전갱이 육으로 제조한 알칼리 수리미의 가열 겔 강도 증강을 위한 최적 전분 및 비근육 단백질의 선정과 이들 성분의 최적 첨가량을 설정하기 위해 2수준 fractional factorial과 mixture design을 실시하였으며, 반응 값은 punch test에 의한 물성과 색차계로 색을 측정하였다. 감자, 옥수수 및 밀전분은 파괴 강도 값을 감소시키지만 변형 값에는 유의적인 영향을 미치지 않는 것으로 나타났다. 소혈청 단백질은 파괴강도 값을 크게 증가시킨 반면 분말 난백 단백질은 다소 효과가 인정되었고 유장단백질과 대두 농축 단백질은 효과가 없었다. 그리고 소 혈청 단백질은 변형값을 다소 증가시킨 반면 분말 난백 단백질을 크게 감소시켰다. 감자와 옥수수 전분과 비근육 단백질은 백색도를 다소 개선하였다. 파괴강도 110g 이상, 변형 값 4.2 mm이상, 백색도 22.5 이상을 보이는 수리미, 옥수수전분 및 소혈청의 최적 혼합 비율은 각각 89.5∼90.0%, 4.6∼6.0%, 4.3∼5.4%의 범위였다. 전분과 비근육 단백질을 첨가하여 제조한 전갱이 알칼리 수리미의 가열 겔이 수세 수리미 겔에 비하여 균일한 단백질 분포를 보이고 있었다.