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http://dx.doi.org/10.5713/ajas.2006.1257

cDNA Cloning, Tissue Expression and Association of Porcine Pleiomorphic Adenoma Gene-like 1 (PLAGL1) Gene with Carcass Traits  

Zhang, F.W. (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture Huazhong Agricultural University)
Cheng, H.C. (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture Huazhong Agricultural University)
Deng, C.Y. (College of Animal Science, Huazhong Agricultural University)
Xiong, Y.Z. (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture Huazhong Agricultural University)
Li, F.E. (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture Huazhong Agricultural University)
Lei, M.G. (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture Huazhong Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.9, 2006 , pp. 1257-1261 More about this Journal
Abstract
Pleiomorphic adenoma gene-like1 (PLAGL1) encodes a zinc-finger (ZF) protein with seven ZFs of the C2H2-type which is a regulator of apoptosis and cell cycle arrest, and also regulates the secretion of insulin. In both human and mouse, PLAGL1 is a candidate gene for tumor suppressor and transient neonatal diabetes mellitus (TNDM). In this study, a 2,238 bp fragment covering the complete coding region was obtained and deposited to GenBank (accession number: DQ288899). The reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that PLAGL1 was expressed almost equally in heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus and ovary. Comparing the sequences of Large White and Meishan pigs, a C-T transition in exon 6 was found. The polymorphism could be detected by TaqI and was genotyped in five purebreds (Large White, Landrace, Meishan, Tongcheng and Bamei). Association analysis was performed between the polymorphism and carcass traits in 276 pigs of a "Large White${\times}$Meishan" F2 resource population. As a consequence, significant associations of the genotypes with shoulder backfat thickness (SFT) and internal fat rate (IFR) were observed. Pigs with TT genotype had low SFT and high IFR compared with TC or CC genotypes.
Keywords
PLAGL1; Porcine; Polymorphism; Carcass Traits;
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1 Bilanges, B., A. Varrault, E. Basyuk, C. Rodriguez, A. Mazumdar, C. Pantaloni, J. Bockaert, C. Theillet, D. Spengler and L. Journot. 1999. Loss of expression of the candidate tumor suppressor gene ZAC in breast cancer cell lines and primary tumors. Oncogene. 18:3979-3988   DOI
2 Gardner, R. J., A. J. Mungall, I. Dunham, J. C. Barber, J. P. Shield, I. K. Temple and D. O. Robinso. 1999. Localisation of a gene for transient neonatal diabetes mellitus to an 18.72 cR3000 (approximately 5.4 Mb) interval on chromosome 6q. J. Med. Genet. 36:192-196
3 Lee, H. K., S. S. Lee and T. H. Kim. 2003. Detection of imprinted quantitative trait loci (QTL) for growth traits in pigs. Asian-Aust. J. Sci. 16:1087-1092   DOI
4 Yang, Q., C. Q. Lai, L. Parnell, L. A. Cupples, X. Adiconis, Y. Zhu, P. W. Wilson, D. E. Housman, A. M. Shearman, R. B. D'Agostino and J. M. Ordovas. 2005. Genome-wide linkage analyses and candidate gene fine mapping for HDL3 cholesterol: the Framingham Study. J. Lipid. Res. 46:1416-1425   DOI   ScienceOn
5 Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Andersson, L. Andersson-Eklund, I. Edfors-Lilja, M. Fredholm and I. Hansson. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Sci. 263:1771-1774   DOI
6 Spengler, D., M. Villalba, A. Hoffmann, C. Pantaloni, S. Houssami, J. Bockaert and L. Journot. 1997. Regulation of apoptosis and cell cycle arrest by Zac1, a novel zinc finger protein expressed in the pituitary gland and the brain. EMBO. J. 16:2814-2825   DOI   ScienceOn
7 Xiong, Y. Z. and C. Y. Deng. 1999. Technical testing rule of lean pig. Chinese Agricultural Press, Beijing
8 Ma, D., J. P. Shield, W. Dean, I. Leclerc, C. Knauf, R. R. Burcelin, G. A. Rutter and G. Kelsey. 2004. Impaired glucose homeostasis in transgenic mice expressing the human transient neonatal diabetes mellitus locus, TNDM. J. Clin. Invest. 114:339-348   DOI
9 Arima, T., T. Kamikihara, T. Hayashida, K. Kato, T. Inoue, Y. Shirayoshi, M. Oshimura, H. Soejima, T. Mukai and N. 2005. ZAC, LIT1 (KCNQ1OT1) and p57KIP2 (CDKN1C) are in an imprinted gene network that may play a role in Beckwith- Wiedemann syndrome. Nucleic. Acids. Res. 33:2650-2660   DOI   ScienceOn
10 Nezer, C., L. Moreau, B. Brouwers, W. Coppieters, J. Detilleux, R. Hanset, L. Karim, A. Kvasz, P. Leroy and M. Georges. 1999. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat. Genet. 21:155-156   DOI   ScienceOn
11 Reik, W., F. Santos and W. Dean. 2003. Mammalian epigenomics: reprogramming the genome for development and therapy. Theriogenol. 59:21-32   DOI   ScienceOn
12 Kamiya, M., H. Judson, Y. Okazaki, M. Kusakabe, M. Muramatsu, S. Takada, N. Takagi, T. Arima, N. Wake, K. Kamimura, K. Satomura, R. Hermann, D. T. Bonthron and Y. Hayashizaki. 2000. The cell cycle control gene ZAC/PLAGL1 is imprinteda strong candidate gene for transient neonatal diabetes. Hum. Mol. Genet. 9:453-460   DOI   ScienceOn
13 Alexander, L. J., D. L. Troyer, G. A. Rohrer, T. P. Smith, L. B. Schook and C. W. Beattie. 1996. Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites. Mamm. Genome. 7:368-372   DOI
14 Koning, D. J., A. P. Rattink, B. Harlizius, J. A. Arendonk, E. W. Brascamp and M. A. Groenen. 2000. Genome-wide scan for body composition in pigs reveals important role of imprinting. Proc. Natl. Acad. Sci. USA. 97:7947-7950
15 Thomsen, H., H. K. Lee, M. F. Rothschild, M. Malek and J. C. Dekkers. 2004. Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine. J .Anim. Sci. 82:2213-2228
16 Zuo, B., Y. Z. Xiong, C. Y. Deng, Y. H. Su, J. Wang, M. G. Lei, F. E. Li, S. W. Jiang and R. Zheng. 2004. cDNA cloning, genomic structure and polymorphism of the porcine FHL3 gene. Anim. Genet. 35: 230-233   DOI   ScienceOn
17 Estelle, J., A. Mercade, J. L. Noguera, M. Perez-Enciso, C. Ovilo, A. Sanchez and J. M. Folch. 2005. Effect of the porcine IGF2- intron3-G3072A substitution in an outbred Large White population and in an Iberian$\times$Landrace cross. J. Anim. Sci. 83:2723-2728
18 Khatib, H. 2004. Imprinting of Nesp55 gene in cattle. Mamm. Genome. 15:663-667   DOI
19 Liu, B. H. 1998. Statistical genomics: linkage, mapping, and QTL analysis. CRC Press, LLC
20 Abdollahi, A., D. Roberts, A. K. Godwin, D. C. Schultz, G. Sonoda, J. R. Testa and T. C. Hamilton. 1997. Identification of a zinc-finger gene at 6q25: a chromosomal region implicated in development of many solid tumors. Oncogene. 14:1973-1979   DOI
21 Zhang, Z. B., M. G. Lei, C. Y. Deng, Y. ZH. Xiong, B. Zuo and F. E. Li. 2005. Lipoprotein lipase gene and productive traits in pig resource family. Asian-Aust. J. Anim. Sci. 18:458-462   DOI
22 Jeon, J. T., O. Carlborg, A. Tornsten, E. Giuffra, V. Amarger, P. Chardon, L. Andersson-Eklund, K. Andersson, I. Hansson, K. Lundstrom and L. Andersson. 1999. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nat. Genet. 21:157-158   DOI   ScienceOn
23 Pira, G., A. El Kharroubi, S. Kozlov, D. Escalante-Alcalde, L. Hernandez, N. G. Copeland, D. J. Gilbert, N. A. Jenkins and C. L. Stewart. 2000. Zac1 (Lot1), a potential tumor suppressor gene, and the gene for epsilon-sarcoglycan are maternally imprinted genes: identification by a subtractive screen of novel uniparental fibroblast lines. Mol. Cell. Biol. 20:3308-3315   DOI