• Food Science and Biotechnology
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• 제15권1호
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• pp.70-76
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• 2006

Anti-obesity effect of biocellulose and diet formulation containing it was evaluated using obese rats. Thirty male Sprague-Dawlely (SD) rats were randomly assigned to high-fat diet group (CON), high-fat diet group containing 5% biocellulose (BIO), and high-fat diet group containing 5% dietary formulation (DF). After 10 weeks, body weight gains of BIO and DF groups were reduced by 15.1 and 6%, respectively, as compared with CON group. Epididymus, parirenal, and visceral fat pads of BIO and DF groups were lower than those of CON group. Weight of interscapular brown adipose tissues increased slightly in BIO group and significantly in DF group. Size of adipocyte in rats decreased in BIO and DF groups. Results indicate biocellulose-containing diet has anti-obesity effect by reducing body weight gain and white adipocytes depots in rats fed high-fat diet.

• 대한치과의사협회지
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• 제18권8호통권137호
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• pp.657-667
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• 1980

This study was undertaken to observe the salivary gland of the white rat when exposed to single and fractionated doses of Cobalt-60 irradiation. One hundred fifty white rats of the experiment were devided into control and 3 experimental groups. In experimental groups, group I receivcd 1200 rads everyweek untill 4800 rads reached, group II received 1500 rads and group III received 2000 rads with single dose. irradiation was carried out using a RAC-120 Cobalt-60 Teletherapy Unit with a dose rate 84.3 r/min, field size 4×5 cm measured at 80 from source. Rats were serially sacrificed at the following postirradiation time intervals: 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42days. At sacrifice, the parotid and submandibular glands were dissected out in toto, and stained with: 1) hematoxylin and eosin; 2) periodic acid Shiff; 3) toludine blue.

• 대한한의학회지
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• 제25권2호
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• pp.87-97
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• 2004

Objectives : To identify the effect of Taeyeumjowee-tang on the treatment of obesity Methods : After having been fed a high-fat diet for 6 weeks, obese white rats were classified into three groups. Control group 1 was fed a high-fat diet for 2 more weeks. Control group 2 was fed a normal diet for 2 weeks. The Sample group was fed Taeyeumjowee-tang extract with a normal diet for 2 weeks. The result of the comparison among the three groups was as follows. Results : The Sample group decreased significantly as compared with the Control 1 and 2 groups in changes of the body weight, fat cell size, fat accumulation, insulin, triglyceride, total cholesterol, total lipid, free fatty acid and UCP1. Conclusions : Taeyeumjowee-tang has significant effect on the treatment of obesity resulting from a high-fat diet. Especially, it was a sure remedy for Control group 2 that was fed normal1y after having a high-fat diet.

• Journal of Ginseng Research
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• 제41권3호
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• pp.347-352
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• 2017

Background: Panax ginseng (PG) has a long history of use in Asian medicine because of its multiple pharmacological activities. It has been considered that PG in a type of white ginseng may induce undesirable thermogenic effects, but not in a type of red ginseng. However, there is a lack of evidence about the correlation between ginsenoside and thermogenesis. Methods: We investigated the effects of PG with different ginsenoside compositions on body temperature, blood pressure, and thermogenesis-related factors in rats. Results: With increasing steaming time (0 h, 3 h, 6 h, and 9 h), the production of protopanaxadiol ginsenosides increased, whereas protopanaxatriol ginsenosides decreased in white ginseng. In both short- and long-term studies, administration of four ginseng extracts prepared at different steaming times did not induce significant changes in body temperature (skin, tail, and rectum) and blood pressure of rats compared to saline control. In addition, there were no significant differences in the molecular markers related to thermogenesis (p > 0.05), mRNA expressions of peroxisome proliferator-activated receptor-gamma coactivator-$1{\alpha}$ and uncoupling protein 1 in brown adipose tissue, as well as the serum levels of interleukin-6, inducible nitric oxide synthase, and nitrite among the treatment groups. Conclusion: These observations indicate that the potential undesirable effects of PG on body temperature could not be explained by the difference in ginsenoside composition.

• 디지털융복합연구
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• 제12권6호
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• pp.433-438
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• 2014

본 연구는 흰쥐의 정소에 방사선을 조사하였을 때 시간이 경과함에 따라 나타나는 정소세포 및 정자의 변화를 현미경학적으로 얻은 영상을 기초로 영상처리에 의한 정량분석을 통하여 정자의 변화에 대한 결과를 정량화함으로써 더 정확하고 객관적인 결과를 얻고자 하는데 그 목적을 두고 있다. 연구에는 8주 수령의 흰쥐를 대상으로 하였으며, 6 MV의 X선을 1회 2 Gy를 전신 조사하였다. 총 30 마리의 흰쥐를 대상으로 방사선을 조사하지 않은 정상대조군과 실험군을 조사 즉시, 조사 2시간, 4시간, 8시간, 24시간 후에 각 실험군 마다 5마리의 정소를 각각 적출하였다. 적출 후 Periodic acid Schiff 염색을 시행하여 정상대조군과 실험군에서의 정소세포와 정자의 상태를 관찰하였다. 방사선조사 후 24시간까지, 정소세포수와 정자수가 점차 감소하는 것을 정성적 및 정량적으로 유의함을 확인하였다.

• 생명과학회지
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• 제20권2호
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• pp.161-168
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• 2010

White-skinned sweet potato (WSP, Ipomoea batatas L.)는 일본, 인도네시아 등에서 당뇨병과 그 치료에 민간에서 사용되고 있다. 본 실험에서는 WSP의 메탄올 추출물을 체중 1 kg 당 Dose 100, 200 mg을 투여하여 streptozotocin으로 유발된 당뇨쥐에서 손상된 신장 보호효과를 평가하였고 그 효능을 인슐린 분비촉진제인 glimepiride (45 mg/kg 체중)와 비교해 보았다. WSP 메탄올 추출물과 glimepiride를 2주 투여 한 후 혈당, 혈중 요소성 질소(BUN), 크레아티닌, 젖산 탈수소효소(LDH), 지질 과산화물(LPO) 함량, 그리고 항산화효소들인 superoxide dismutase (SOD), 카탈라아제(CAT), 글루타치온 과산화물 분해효소(GPx), 글루타치온 S-전이효소(GST) 등의 활성도를 측정하였다. BUN, 크레아티닌, LDH, 혈당, LPO 함량 등은 대조군에 비하여 그 값이 증가하였고, SOD, CAT, GPx, GST 값은 감소하였다. WSP 메탄올 추출물(200 mg/kg)을 투여한 후 측정한 값은 혈당, LPO, 신장병 표지인자인 BUN, 크레아티닌, LDH, 그리고 지질 과산화물 함량에서 의미있는 개선효과를 볼 수 있었고 항산화효소, 항산화물질의 증가도 나타났다. 따라서 WSP 메탄올 추출물은 당뇨쥐의 혈당을 낮추며 산화적 스트레스를 약화시키고 당뇨로 유발된 신장 손상을 보호해 주는 효과가 있다는 결과를 얻었다. 또한 민간에서 사용하고 있는 WSP가 실제로 당뇨 치료에 효과가 있음을 과학적 증거로 제공해 주었다고 판단된다.

• Journal of Periodontal and Implant Science
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• 제10권1호
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• pp.37.1-37.1
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• 1980

• Maxillofacial Plastic and Reconstructive Surgery
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• 제20권2호
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• pp.158-165
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• 1998

This study was undertaken to observe the effects of the antineoplastic agent, 5-Fluorouracil(5 FU) on the hair in Sprague-Dawley white rats. Twenty four sprague-Dawley strain white rats, each weighing about 150-200 grams were used and divided into control and experimental groups. In the experimental group, eighteen rats were injected intraperitonially with 60 mg of 5-FU per killogram body weight with one time per two days, Six rats were injected with 0.5 cc of normal saline solution intraperitoneally as a placebo on this control group. Rats were serially sacrificed on the first, third, fifth, seventh, tenth and fourteenth day after 2 times of injection of 5-FU and saline. The hair were obtained and observed SEM. After examination and comparision of all specimens, the results of this study were as follows: 1. In the control group, the scale and cuticle of hair was observed smooth surface and equal interval 2. In the experimental group, the first day, scale change was seen from body of hair and crack was seen. from fifth day, and irregular scale and cuticle of hair was seen from 10, 14 days 3. The apperance of root of hair was not almost change From above results, 5-Fluorouracil was more effective on the hair body. The change was begun from first day and crack of scale was seen from fifth day and irregular scale and cuticle of hair was seen from 10,14 days. The.

• 한방재활의학과학회지
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• 제21권3호
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• pp.1-11
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• 2011

Objectives : This study was performed to evaluate the effects of root of Scutellariae Radix(SR) water extract against inflammatory response in the spinal cord injury(SCI). Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rat. SR was orally given once a day for 7days after SCI. Myeloperoxidase(MPO) positive neutrophils infiltration was examined. Inducible nitric oxide synthase(iNOS) and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) expressions were observed with immunohistochemistry. Glial fibrillary acidic protein(GFAP) positive astrocytes were examined using immuno-fluorescence. Results : 1. SR reduced MPO-positive neutrophils infiltration in peri-damage regions of the contusive SCI-induced rats. 2. SR reduced iNOS positive cells in the white matter of the contusive SCI-induced rats. 3. SR reduced TNF-${\alpha}$ positive cells in the gray and white matter of the contusive SCI-induced rats. 4. SR reduced cell number and size of astrocytes in peri-damage regions of the contusive SCI-induced rats. Conclusions : These results suggest that SR plays an inhibitory role against inflammatory response in the SCI.

• 대한수의학회지
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• 제23권1호
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• pp.57-67
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• 1983

The present study was carried out to observe the histopathological changes in the mammary gland of lactating rats and rabbits injected with dexamethasone. White rats were intramuscularly injected with 0.25mg, 0.5mg or 1.0mg of dexamethasone sodium phosphate (containing $9{\alpha}$-fluoro-$16{\alpha}$-methylprednisolone, 5.0mg/ml) daily for 3 to 10 days on the 3rd day after parturition and white rabbits were intramammary infused with 4mg or 20mg of dexamethasone daily for 4 days on 7th day after parturition. The histopathological changes of the mammary glands, ovaries and adrenal glands of rats and rabbits were observed with light microscope. In the mammary glands of rats, the microscopic findings encountered were decrease of the milk in the alveolar lumina, necrosis and desquamation of epithelial cells, atrophy of alveoli, proliferation of fibroblasts and thickness of alveolar walls, destruction of alveoli, presence of fat droplets within the glandular epithelial cells, infiltration of mononuclear cells and proliferation of adipose tissue, which were relative to the dose and duration of injection. Especially, in the cases of the administration of large doses or long duration, there were severe fibrosis and focal necrosis of glandular tissue. In the mammary glands of rabbits, the morphological changes were similar to those findings in the rats. The milk in the alveolar lumina was decreased gradually according to the dose and duration of injection, while milk fat concentration regarded to increase. In the histological findings of ovaries, necrosis of granulosa cellos, vacuolization and necrosis of luteal cells, atrophy and necrotic foci in the corpora lutes were observed. In the adrenal glands, hyperemia, hemorrhage, vacuolization of adrenal cortical cells, necrotic foci and atrophy of adrenal cortex were observed.