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Effects of Root of Scutellariae Radix against Inflammatory Response in the Spinal Cord Contusion Injury in Rats  

Yang, Kee-Young (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Choi, Won-Ik (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Shin, Jung-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Park, Seong-Ha (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University)
Kim, Seong-Joon (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Lee, Jong-Soo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Hee University)
Sohn, Nak-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.21, no.3, 2011 , pp. 1-11 More about this Journal
Abstract
Objectives : This study was performed to evaluate the effects of root of Scutellariae Radix(SR) water extract against inflammatory response in the spinal cord injury(SCI). Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rat. SR was orally given once a day for 7days after SCI. Myeloperoxidase(MPO) positive neutrophils infiltration was examined. Inducible nitric oxide synthase(iNOS) and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) expressions were observed with immunohistochemistry. Glial fibrillary acidic protein(GFAP) positive astrocytes were examined using immuno-fluorescence. Results : 1. SR reduced MPO-positive neutrophils infiltration in peri-damage regions of the contusive SCI-induced rats. 2. SR reduced iNOS positive cells in the white matter of the contusive SCI-induced rats. 3. SR reduced TNF-${\alpha}$ positive cells in the gray and white matter of the contusive SCI-induced rats. 4. SR reduced cell number and size of astrocytes in peri-damage regions of the contusive SCI-induced rats. Conclusions : These results suggest that SR plays an inhibitory role against inflammatory response in the SCI.
Keywords
Spinal cord injury; Scutellariae Radix; Anti-inflammation; Neutrophil infiltration; iNOS; TNF-${\alpha}$;
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