• Journal of Marine Bioscience and Biotechnology
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• v.2 no.2
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• pp.108-112
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• 2007

A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

• Wind and Structures
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• v.24 no.6
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• pp.613-635
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• 2017

Strong downslope windstorms often occur in the Liguria Region. This part of North-Western Italy is characterised by an almost continuous mountain range along its West-East axis consisting of Maritime Alps and Apennines, which separate the Padan Plain to the North from the Mediterranean Sea to the South. Along this mountain range many valleys occur, frequently perpendicular to the mountain range axis, where strong gap flows sometimes develop from the top of the mountains ridge to the sea. In the framework of the European projects "Wind and Ports" and "Wind, Ports, and Sea", an anemometric monitoring network made up of 15 (ultra)sonic anemometric stations and 2 LiDARs has been realised in the three main commercial ports of Liguria. Thanks to this network two investigations are herein carried out. First, the wind climatology and the main statistical parameters of one Liguria valley have been studied through the analysis of the measurements taken along a period of 4 years by the anemometer placed at its southern exit. Then, the main characteristics of two strong gap flows that occurred in two distinct valley of Liguria are examined. Both these studies focus, on the one hand, on the climatological and meteorological characterisation of the downslope wind events and, on the other hand, on their most relevant quantities that can affect wind engineering problems.

• Architectural research
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• v.22 no.4
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• pp.135-143
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• 2020

The Netherlands is a human-made country and an extremely well-designed European country as well. The general Dutch spatial planning for the city and environment takes place at a regional level. The local community determines the primary development conditions, and the prospect is included in a legally binding land-use plan. Especially, Rotterdam is a representative port city as the center of world trade and the gateway to western Europe. According to the history of war, the city reconstruction and the movement of the port area have led to a general change in Rotterdam and the regional redevelopment project on the southern port area of Mass river for the expansion of city functions and the balanced development. The research purpose is to understand the spatial development of the Netherlands city-region based on the analysis regarding the Kop van Zuid project, which is a representative implemented case in Rotterdam. The theoretical framework is the five dimensions and twelve indicators of territorial governance from the TANGO research project by the EU. The target case is assessed by planning and social aspect, respectively, and the results are discussed based on the theoretical framework. This research has the possibility to be utilized as advanced research by the European perspective for spatial development in other city-regions with the port area, such as Incheon and Busan in Korea.

• Journal of Korea Society of Digital Industry and Information Management
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• v.20 no.1
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• pp.149-160
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• 2024

This paper was conducted to improve understanding of how the start-up process affects the local community and how young entrepreneurs build and grow their businesses in the process through analysis of young people who started businesses in the region. The research method was conducted through in-depth interviews with 10 young entrepreneurs who started businesses in the western Gyeongsangnam-do region, where population decline is prominent, and the collected data was analyzed through a grounded theory approach to derive common and meaningful themes between each case. proceeded. As a result of the study, insights were derived about the background of entrepreneurs settling in the region, the motivation for starting a business using local resources, the impact of government and local government support, business strategies for sustainable growth, and the entrepreneurs' personal performance. Through this analysis, we hope to provide important insights into the positive impact local entrepreneurship has on the community and how young entrepreneurs contribute to local development.

• Ocean and Polar Research
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• v.35 no.3
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• pp.259-272
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• 2013

One of the factors influencing the climate around Korea is the oceanic-atmospheric variability in the tropical region between the eastern Indian and the western Pacific Oceans. Lack of knowledge about the air-sea interaction in the tropical Indo-Pacific region continues to make it problematic forecasting the ocean climate in the East Asia. The 'Tropical Indo-Pacific water transport and ecosystem monitoring EXperiment (TIPEX)' is a program for monitoring the ocean circulation variability between Pacific and Indian Oceans and for improving the accuracy of future climate forecasting. The main goal of the TIPEX program is to quantify the climate and ocean circulation change between the Indian and the Pacific Oceans. The contents of the program are 1) to observe the mixing process of different water masses and water transport in the eastern Indian and the western Pacific, 2) to understand the large-scale oceanic-climatic variation including El Nino-Southern Oscillation (ENSO)/Warm Pool/Pacific Decadal Oscillation (PDO)/Indian Ocean Dipole (IOD), and 3) to monitor the biogeochemical processes, material flux, and biological changes due to the climate change. In order to effectively carry out the monitoring program, close international cooperation and the proper co-work sharing of tasks between China, Japan, Indonesia, and India as well as USA is required.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.27 no.1
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• pp.25-37
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• 2015

Tide-surge interaction during typhoon periods has been analyzed. The quantitative analysis of the Chi-square test shows that the interaction is most prominent at the Southwestern coast whereas the Western and the Southeastern regions are not. Patterns of surge type were divided into two groups, that is, steep type and mild type. Then, the interaction was turned out to be more prominent for mild type data. The weak interaction at the Western region is considered due to negative surges when the south-track typhoons attack. However, the interaction is remarkable when the west-track typhoons attack. The weak interaction at the Southeastern coast is, on the other hand, considered due to abundance of the steep type typhoons. Thus, inundation risk would be so apprehensive at that region because large-scale surge might be caused even at high tide.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.97-97
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• 2003

For many animals the centrosome consists of a pair of centrioles and surrounding pericentriolar materials (PCMs). PCMs have been known to play roles during cell division. It is known that centrioles are necessary to assemble centrosomal components. However, many types of oocytes undergo meiosis without centrioles. It is known that in nonmurine mammalian species, the sperm introduces an intact proximal centriole unlike sea urchin where two centrioles are introduced. In case of mouse sperm, the presence of centrosome is not clear In this study, a monoclonal antibody was developed to investigate centrosome during mouse germ cell and early embryo development. Results of immunostaining and Western blotting in CHO cells suggest that the monoclonal antibody recognizes a nuclear and centrosomal protein, thus called NCP. The NCP monoclonal antibody was used to screen a cDNA expression library prepared from 12.5 mouse brain to isolate NCP gene. Nucleotide size of NCP gene obtained from immunoscreening was about 5.5kb. It is determined that the NCP may be closely related with pericentriolar material -1 gene (Pcm-1) from the result of sequencing analysis. The molecular weight, 66kDa, calculated by known DNA sequence in database is consistent with that of detected from Western blotting using CHO cell lysates. Therefore, it is assumed that NCP may be alternative splicing form of Pcm-1 of which molecular weight is 228kDa. In mouse oocytes, NCP was distributed in nucleus as in CHO cells. It was shown that the NCP was localized around neck region, probably the centrosome in mouse neck region. Interestingly, dramatic change in distribution of NCP was also shown in male germ cell development. Finally, we observed the cellular distribution of NCP during early embryo development. NCP was detected in nucleus as well as centrosome foci. It is suggested that the centrioles reassembly we occurring in blastocysts and then affects the distribution of NCP.

• Ocean and Polar Research
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• v.36 no.1
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• pp.1-12
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• 2014

This study investigates spatial and temporal variations in the generation and propagation of internal tides around the Korea Strait using a three-dimensional high resolution model (Regional Ocean Modeling System; ROMS). The model results were verified through comparison with in-situ current measurements from an array of 12 acoustic Doppler current profilers (ADCPs) deployed in the Korea Strait. Fluxes and distributions of internal tidal energy were calculated using simulation results gathered in February and August. Our analyses reveal that energetic semidiurnal internal tides are generated in a region around the Korea Strait shelf break ($35.5^{\circ}N$, $130^{\circ}{\sim}130.5^{\circ}E$), where the strong cross-slope semidiurnal barotropic tidal currents interact with a sudden topographical change. The semidiurnal internal tidal energy generated in summer displays values about twice as large as values in winter. Propagation of semidiurnal internal tides also reveals seasonal variability. In February, most of the semidiurnal internal tides propagate only into the open basin of the East Sea due to weak stratification in the Korea Strait, which inhibits their southwestward propagation. In August, they propagate southwestward to $35.2^{\circ}N$ along the western channel of the Korea Strait because of strong stratification. In addition, semidiurnal internal tides generated in a region west of Tsushima Island are found to propagate to the coast of Busan. This can be explained by the intensified stratification due to the strong intrusion of bottom cold water in the western channel of the Korea Strait during summer.

• Ocean and Polar Research
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• v.23 no.4
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• pp.361-376
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• 2001

The characteristics of SST variability in the East Sea are analyzed using NOAA/AVHRR weekly SST data with about $0.18^{\circ}{\times}0.18^{\circ}$ resolution ($1981{\sim}2000$) and reconstructed historical monthly SST data with $2^{\circ}{\times}2^{\circ}$ resolution $(1950{\sim}1998)$. The distinct feature of wintertime SST is high variability in the western and eastern parts of $38^{\circ}{\sim}40^{\circ}$ latitudinal band, which are the northern boundary of warm current in the East Sea during winter. However, summertime SST exhibits variability with similar magnitude in the entire region of the East Sea. The analysis of remote correlation also shows that SST in the East Sea is closely correlated with that in the region of Kuroshio in winter, but in summer is related with that in the western and eastern regions of the same latitudes. From these results it is postulated that the SST variability in the East Sea may be related with the variations of East Korean Warm Current and Tsushima Warm Current in winter, but in summer probably with the variations of atmospheric components. In the analysis of ENSO related SST anomaly, a significant negative correlation between SST anomalies in the East Sea and SST anomalies in the tropical Pacific is found in the months of August-October (ASO). The SST in the ASO period shows more significant cooling in E1 $Ni\~{n}o$ events than warming in La $Ni\~{n}a$ events. Also, the regional analysis shows by the Student's t-test that the negative SST anomalies in the E1 $Ni\~{n}o$ events are more significant in the southwestern part of the East Sea.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4289-4294
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• 2014

Background: One of the most important reasons for the high mortality rates of cancer is the low level of awareness, which can lead to a late diagnosis and treatments starting too late. Therefore, it is necessary for individuals, especially at younger age, to gain awareness and integrate taking the necessary precautions into their lifestyle in order to prevent cancer and ensure early diagnosis. The aim of this study was to assess the levels of awareness of factors for major cancers among students studying in two different campuses of a university in the Western Black Sea Region. Materials and Methods: This descriptive/cross-sectional study was performed between January-March 2014. Students studying in the 2013-2014 academic year in a university in the Western Black Sea Region in two separate campuses were the subjects. Results: The mean age was $21.01{\pm}3.63$. While female students (51.2%) were the majority in the health sciences campus (74.8%), male students (48.8%) were the majority in the social sciences campus (76.5%). Some 9.6% of the students from the health sciences campus and 12.4% of the students from the social sciences campus thought that cancer was communicable, while most of the students from both campuses knew that smoking caused cancer. It can also be seen that the rate of answering questions regarding cancer correctly was higher among students studying in the health sciences campus. Conclusions: It was determined that students who do not study at the health sciences campus have insufficient information on cancer, cancer symptoms, and the possibility of breast cancer in males and approximately half of them regarded obesity as a risk factor for cancer.