• Journal of Korea Technology Innovation Society
• /
• v.12 no.4
• /
• pp.840-864
• /
• 2009

At the time of so-called localization, the start-up and successful management of NTVs (New technology ventures) might be a key factor of regional economic development. The research investigated the factors influencing the performance of NTVs South-western Area. According to the research result, the capability of CEO and R&D cooperation, supply chain integratim, information searching activities, market orientation are the factors which have positive correlation with the performance of NTVs in a statistically significant way in this region.

• The Plant Pathology Journal
• /
• v.32 no.5
• /
• pp.452-459
• /
• 2016

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-${\beta}$-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting. The expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonal antibodies and application for the virus detection.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.15 no.6
• /
• pp.2031-2048
• /
• 2021

Not only can air pollution reduce the overall competitiveness of tourist destinations, but also changes tourists' travel decisions, thereby affecting the tourism flows. The study presents a machine learning method to analyze how the haze pollution puts spatial effect on tourism flows in China from 2001 to 2018, and reveals the regional differences in heterogeneity among eastern, central, and western China. Our investigation reveals three interesting observations. First, the Environmental Kuznets Curve of the impact of haze pollution on tourism flows is not significant. In the eastern and western regions, the interaction between haze pollution and domestic tourism flows as well as inbound tourism flows shows an inverted U-shaped curve respectively. Second, there is an significantly positive spillover effect of tourism flows in all of the eastern, central, and western regions. As to the intensity of spillover, domestic tourism flows is higher than that of the inbound tourism flows. Both of the above figures are greatest in the eastern. Third, the Chinese haze pollution mainly reduces the inbound tourism flows, and only imposes significantly negative direct effects on the domestic tourism flows in the central region. In the central and eastern regions, significantly negative direct effects and spillover effects are exerted on inbound tourism.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.6
• /
• pp.912-918
• /
• 2003

Mitotic centromere-associated kinesin (MCAK), which is a novel kinesin with a central motor domain, is believed to playa role in mitotic segregation of chromosome during the M phase of the cell cycle. In the present study, it is shown that a rabbit polyclonal antibody has been produced using the N-terminal region (187 aa) of human MCAK expressed in E. coli as the antigen. To express the N-terminal region in E. coli, the MCAK cDNA fragment encoding N-terminal 187 aa was obtained by PCR and was then inserted into the pET 3d expression vector. Molecular mass of the N-terminal region overexpressed in the presence of IPTG was 23.2 kDa on SDS-PAGE, and the protein was insoluble and mainly localized in the inclusion body that could be easily purified from the other cellular proteins. The N-terminal region was purified by electro-elution from the gel after the inclusion body was resolved on the SDS-PAGE. The antiserum obtained after tertiary immunization with the purified protein specifically recognized HsMCAK when subjected to Western blot analysis, and showed a fluctuation of the protein level during the cell cycle of human Jurkat T cells. Synchronization of the cell-cycle progression required for recovery of cells at a specific stage of the cell cycle was performed by either hydroxyurea or nocadazole, and subsequent release from each blocking at 2, 4, and 7 h. Northern and Western analyses revealed that both mRNA and protein of HsMCAK reached a maximum level in the S phase and declined to a basal level in the G1 phase. These results indicate that a polyclonal antibody raised against the N-terminal region (187 aa) of HsMCAK, overexpressed in E. coli, specifically detects HsMCAK (81 kDa), and it can analyze the differential expression of HsMCAK protein during the cell cycle.

• Wind and Structures
• /
• v.9 no.6
• /
• pp.419-440
• /
• 2006

This article synthesizes the literature on the meteorology, experimental simulation, and wind engineering ramifications of intense downburst outflows. A novel design of a large-scale test facility and experimental evidence of its validity are presented. A two-dimensional slot jet is used to simulate only the outflow region of a downburst. Profiles of mean velocity and turbulence quantities are acquired using hot-wire anemometry. Comparison with the literature provides empirical evidence that supports the current approach. A geometric analysis considers the validity of applying a two-dimensional approximation for downburst wind loading of structures. This analysis is applicable to power transmission lines in particular. The slot jet concept can be implemented in a large boundary layer wind tunnel to enable large-scale laboratory experiments of thunderstorm wind loads on structures.

• Atmosphere
• /
• v.25 no.1
• /
• pp.179-183
• /
• 2015

Even though state-of-the-art general circulation models is improved step by step, the seasonal predictability of the East Asian summer monsoon still remains poor. In contrast, the seasonal predictability of western North Pacific and Indian monsoon region using dynamic models is relatively high. This study builds canonical correlation analysis model for seasonal prediction using wind fields over western North Pacific and Indian Ocean from the Global Seasonal Forecasting System version 5 (GloSea5), and then assesses the predictability of so-called hybrid model. In addition, we suggest improvement method for forecast skill by introducing the lagged ensemble technique.

• ALGAE
• /
• v.28 no.1
• /
• pp.53-62
• /
• 2013

Actinotrichia is a calcified galaxauracean red algal genus with temperate and tropical distributions in the Indian and Pacific Oceans. Morphological characteristics, along with rbcL and cox1 sequences, were analyzed from specimens collected in the western Pacific and the Indian Oceans. Both rbcL and cox1 data confirmed the occurrence of A. fragilis, A. robusta, and Actinotrichia sp. in this region. The presence of A. fragilis was verified in tropical Indo-Pacific and temperate northeast Asian waters and was characterized by high genetic diversity. Although A. robusta commonly occurs in the East China Sea, we confirmed its presence on rocks and crustose algae in the subtidal zone of three islands in the Andaman Sea. Actinotrichia sp. was similar to A. calcea in morphology and distribution, but with sufficiently different sequences, thus, additional sampling over the range will enable a more realistic evaluation of its taxonomic status.

• Journal of Mechanical Science and Technology
• /
• v.15 no.6
• /
• pp.764-775
• /
• 2001

Oscillartory thermocapillary flow of high Prandtl number fluids in the half-zone configuration is investigated. Based on experimental observations, one oscillation cycle consists of an active period where the surface flow is strong and the hot corner region is extended and a slow period where the opposite occurs. It is found that during oscillations the deformation of free surface plays an important role and a surface deformation parameter S correlates the experimental data well on the onset of oscillations. A scaling analysis is performed to analyze the basic steady flow in the parametric ranges of previous ground-based experiments and shows that the flow is viscous dominant and is mainly driven in the hot corner. The predicted scaling laws agree well with the numerical results. It is postulated that the oscillations are caused by a time lag between the surface and return flows. A deformation parameter S represents the response time of the return flow to the surface flow.

• The Korean Journal of Quaternary Research
• /
• v.21 no.2
• /
• pp.77-79
• /
• 2007

The source regions of Asian Dusts (Yellow Sands) in the western part of China are investigated, and the soil samples are collected samples for approximately 15 days during the spring of 2005. Particle sizes of sediments are analyzed and compared with each other. These grain-size analyses from the source areas can be compared Particle sizes between loess deposits and desert sands in western part of China and desert areas show distinctive differences. Loess deposits are predominantly composed of fine sands and silts. The distinction between the final characteristics of Asian Dust particles arrived in Korea and characteristics during transportation can be recognized comparison with the Asian Dust particles collected where the dust particles settled down. The characteristics of Asian Dust particle sizes can provide the basic information regarding the transportation history from the source region.

• Journal of Environmental Science International
• /
• v.14 no.5
• /
• pp.463-471
• /
• 2005

This study is designed to investigate the characteristics of $PM_{10}$ concentration and the chemical composition of heavy metallic components in the $PM_{10}$ sampled in western Busan from March to May, 2003. $PM_{10}$measurement was done during springtime of 2003, totaling 29 days: 9 days in March, 10 days in April and 10 days in May. With a sampling time of 24 hours, it started 9:00 AM on that day and ended 9:00 AM the next day. The mean contribution ratio of soil during springtime was $10.3\%$. Al had a significant correlation with Ca, Fe, Mg and Si and little correlation with Na, Ni and Zn.