• Asian Pacific Journal of Cancer Prevention
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• 제16권11호
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• pp.4543-4548
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• 2015

Background: To investigate the effects of ASMase mediated endothelial cell apoptosis in multiple hypofractionated irradiations in CT26 tumor bearing mice. Materials and Methods: Thirty-five CT26 tumor bearing mice were subjected to single ionizing radiation (IR) of 0, 3, 6, 9, 12, 15, 18 Gy. Eight hours after IR, the mice were sacrificed and tumor tissues were used for CD31 immunohistochemistry staining, TUNEL and CD31 double staining, ASMase activity assay. Then 6 and 12 Gy were chosen for multiple hypofractionated IR experiments according to the above results. Each time after IR, 5 mice were sacrificed and assayed as above. Results: The ASMase activities were increased significantly after a single IR of 12 Gy or higher which was accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. For 6 Gy which was not high enough to trigger ASMase activation, after 2 or more times of IR, the ASMase activities were significantly increased accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. While for 12 Gy, after 2 or more times of IR, the ASMase activities and endothelial cell apoptosis rates were maintained without remarkable increase; however, the MVD was significantly decreased. What's more, the cancer cell apoptosis rates were significantly increased after multiple IR for both 6 Gy and 12 Gy. Conclusions: ASMase mediated endothelial cell apoptosis may play an important role in the process of multiple hypofractionated IR for CT26 colorectal carcinoma.

• 복식
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• 제3권
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• pp.29-46
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• 1980

The purpose of this thesis is to find out how the upper garment styles in the Ancient East and est had been influenced with each other. Analytical studies conclude the fellowing findings: 1) Upper garment styles in the feat Asia and the Egypt already highly developed in 28th century B.C. and show us the original style of the wrap-over to the left and that of the round neckline(曲領). Upper garment of the open in the center front shown in Babylonia in 18th century B.C. had been inherited to the caftan of the Hebrew and later succeeded to the Persia. 2) The tunic styles of the round neckline, the wrap-over to the left and the open in the cotter front, which were the basic styles of the upper garment, had teen widely accepted to the central Asia and the East Asia, as well as the Northern Europe, from the West Asia. 3) The styles of the wrap-over to the right originated from China since it had begun to show in the Shang Dynasty(商代, 殷代). 4) The East and the West costumes had been very much intermixed in 4th century B.C. Alexander the Great of Macedoria in 4th century B.C. expanded his territory to the central Asia and built up the Bacteria, when the most western civilization had been greatly transmitted to the Orient. Meanwhile the tunic being clad in the West and Central Asia began to be worn by soldiers in the period of the Warring States in China (326-299 B.C.) and afterwards worn even by civil officials since the age of the T'ang Dynasty of China. 5) The Upper garments of the open in the center front, the wrap-over to the right, the wrap-over to the left and the round neckline were found in Korea, which mean that the upper garment styles in the Ancient Korea were intermixed of the factors from the West Asia, the central Asia and the East Asia. 6) The styles of costume in the East Asia were influenced by the West Asia through the central Asia. The upper garment styles Europe were also influenced by the West Asia. Thus the upper garment styles in the Ancient East and West had been mutually affected with each other.

• BMB Reports
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• 제43권6호
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• pp.400-406
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• 2010

Cancer/testis (CT) antigens exhibit highly tissue-restricted expression and are considered promising targets for cancer vaccines. Here we identified a novel CT gene ZNF645 which restrictively expresses in normal human testes and lung cancer patients (68.3%). To investigate the promoter methylation status of ZNF645, we carried out bisulfite genomic sequencing and found that the CpG island in its promoter was heavily methylated in normal lung tissues without the expression of ZNF645, whereas there was high demethylation in normal human testes and lung carcinoma tissues with its expression. Also ZNF645 could be remarkably activated in A549 and HEK293T cells treated by DNA demethylation agent 5'-aza-2'-deoxycytidine. And the dual luciferase assay revealed that the promoter activity of the ZNF645 was inhibited by methylation of the CpG island region. Therefore, we proposed that ZNF645 is a CT gene and activated in human testis and lung cancers by demethylation of its promoter region.

• 동의생리병리학회지
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• 제23권5호
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• pp.980-985
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• 2009

The study was designed to test anti-platelet effect and find out anti-platelet mechanism of extract from Gamisopunghwalheol-tang in vitro. The extract was investigated for the inhibition against the aggregation of human platelet suspensions induced from collagen by aggregometer. And also the extract was investigated for the inhibition against the aggregation of human platelet suspensions who is taking aspirin or clopidogrel induced from collagen by aggregometer. In collagen-induced platelet aggregation test, the extract significantly inhibited collagen-induced platelet aggregation in a concentration-dependent manner(p<0.05). The extract significantly inhibited collagen-induced platelet aggregation of human platelet who is taking aspirin or clopidogrel ing 0 mg/ml concentration(p<0.05). And the extract inhibited more ingpatients who is taking aspirin. These results show that the extract from Gamisopunghwalheol-tang has anti-platelet aggregation effect.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.877-881
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• 2014

Objective: To evaluate risk factors for gallstones after gastrectomy. Methods: To identify documents published from 1990 to 2011 the Pubmed, Cochrane Library, Springer Link, CBM and WanFang databases were searched and a meta-analysis was performed with RevMan 5.2 software for odds ratios and 95%CIs. Results: Fifteen studies were selected for the meta-analysis. The pooled ORs [95%CIs] were 0.56 [0.43, 0.73], (P<0.0001) for digestive tract reconstruction, 0.80 [0.54, 1.17], (P=0.25) for pylorus preservation, 0.33[0.15, 0.75], (P=0.008) for resection scope of stomach, 0.33 [0.15, 0.75], (P=0.008) for lymphadenectomy, and 0.13 [0.05, 0.33], (P<0.0001) for vagotomy. Conclusions: Digestive tract physical reconstruction and vagus nerve preservation can reduce the morbidity of gallstones after gastrectomy. Total gastrectomy can add to the morbidity of galltones as does increasing the degree of lymph node dissection. There was no significant difference in gallstones with or without pylorus preservation.

• BMB Reports
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• 제41권9호
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• pp.664-669
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• 2008

Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.

• BMB Reports
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• 제43권9호
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• pp.635-641
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• 2010

Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control in germline from Drosophila to mammals. To examine the profile of small RNA expression in human cancer cells and explore difference in small RNA transcriptome, small RNA libraries prepared from wildtype, HILI overexpressed and HILI knockdowned Hela cells were sequenced using Solexa technology. piRNAs and other repeat-associated small RNAs were observed in Hela cells. By using in situ hybridization, piR-49322 was localized in the nucleolus and around the periphery of nuclear membrane in Hela cells. Following the overexpression of HILI, the retrotransposon elements LINE1 was significantly repressed, while LINE1-associated small RNAs decreased in abundance. The present study demonstrated that HILI along with piRNAs plays a role in LINE1 suppression in Hela cancer cell line.

• 한국지역사회생활과학회지
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• 제16권4호
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• pp.125-137
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• 2005

The purpose of this study was to analyze the regional ripple effects and residents' perceptions of the West Coast Highway on four regions of Chung-nam province. The data was collected through questionnaire interviews with 400 residents in four regions of the west coast area. After data cleaning, the statistical methods used were confirmative factor and cluster analysis with 309 sampled from four regions of Dangjin, Boryong, Seosan and Taean. The results of this study were as follows. First, it is classified, according to the residents' perceptions against the opening of the West Coast Highway, into four groups. Group A is the people who haven't lived there long and mainly consists of relatively young (30's-40's) males, while Group B is the people with lower incomes and middle-aged males (40's) who have lived there for 11 to 20 years. Group C is mainly the people who are middle-aged housewives in their 40's and who haven't lived there long and with a relatively higher income. Group D is the people who lived for less than five years and had lower incomes than other groups. The results show that their perceptions against the influence of regional development differ demographically from each other. In conclusion, it was determined that most of them were concerned that it, coupled with the construction of the West Coast Highway, would damage the surrounding countryside but believed it would contribute to the growth of the region's economy. Whereas groups A and B seem to acknowledge the necessity of regional development and environmental protection, groups B and D appear to be more content with the tourism development derived from the West Coast Highway and are paying more attention to such plans than other two groups.

• Animal cells and systems
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• 제5권2호
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• pp.157-161
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• 2001

Mice homozygous for the lethal autosomal recessive anorexia mutation (anx) present with premature death around postnatal day 22. The anorexia mutant mice also present phenotypes such as reduced body weight, decreased food intake, and abnormal behavior characteristics such as body tremors, hyperactivity, uncoordinated gait, and head weaving. In order to investigate the expression of c-Fos in the hippocampus of anorexia mutant mice, the immunohistochemistry was performed in this study. The anorexia mutant mice exhibited lower expression of c-Fos in the hippocampus regions thBn the control group. In the CA3 and dentate gyrus, the number of c-Fos-positive cells in anorexia mutant mice was noticeably lower than that in control mice. However, no significant difference was found in the number of c-Fos-positive cells in CA1 of the two groups. The result suggests that the phenotypic characteristics of anorexia mutant mice may be associated with the hippocampal deficits of c-Fos expression.