• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.71-77
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• 2012

Many of biochemical or physiological processes can be regulated by non-coding RNAs as well as coding RNAs in plants, animals and microbes. Recently, many small RNAs including microRNAs (miRNAs) and endogenous small interference RNAs (siRNAs) and long non-coding RNAs have been discovered from ubiquitous organisms including plants. Biotic and abiotic stresses are main causal agents of crop losses all over the world. Much efforts have been performed for understanding the complex mechanism of stress responses. Up to date, many of these researches have been related with the identification and investigation of stress-related proteins, showing limitation to resolve the complex mechanism. Recently, non-coding RNAs as well as coding genes have been gradually interested because of its potential roles in plant stress responses as well as other biophysical aspects. In this review, various potential roles of non-coding RNAs, especially miRNAs and siRNAs, are reviewed in relation with plant biotic and abiotic stresses.

• Journal of Periodontal and Implant Science
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• 제37권sup2호
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• pp.447-463
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• 2007

치주인대세포는 시험관적 실험에서 광물화 결절형성을 유도할 수 있으므로 광물화 결절형성에 관여하는 유전자들을 특이하게 발현할 것으로 여겨진다. 이에 본 실험은 cDNA microarray를 이용한 동시 유전자분석을 시행하여 치주인대세포의 분화에 의한 광물화 결절형성시 나타나는 유전자의 특징적 발현 양상을 알아보고자 하였다. 교정치료를 목적으로 경북대학교병원에 내원한 환자의 제일소구치를 발치하여 통상적 방법으로 치주인대세포를 분리, 배양하였고, 3세대의 치주인대세포를 사용하여 실험을 시행하였다. 치주인대세포를 100mm 배양접시에 넣고 배양하여 매 2일 마다 배지를 교환해 주고, 10% FBS만을 투여한 대조군으로, ascorbic acid $(50\;{\mu}g/ml)$, ${\beta}-glycerophosphate$ (10 mM) 및 100 nM dexamethasone을 투여한 군을 실험군으로 하였다. 배양된 치주인대세포에 ascorbic acid, ${\beta}-glycerophosphate$, 그리고 dexamethasone을 투여한 실험군에서 21일째 광물화된 결정을 관찰할 수 있었으나 대조군에서는 관찰할 수 없었다. 3063개의 유전자를 분석한 결과 35개 유전자가 대조군에 비해 2배이상 발현이 증가하였고, 38개 유전자는 2배이상 발현이 감소하였다. 형태학적 검사에서 보여준 바와 같이 광물화 형성과정시 관여하는 JGF-2과 IGFBP2와 같은 유전자가 실험군에서 증가하였으며, 세포골격과 세포외기질 형성에 관여하는 proteogycan 1, fibulin-5, keratin 5, ${\beta}-actin$, ${\alpha}-smooth$ muscle actin, capping protein 등도 발현이 실험군에서 증가하였다. 한편 periostin and S100 calcium-binding protein A4는 대조군에서 오히려 높게 나타나므로 이는 배양된 치주인대세포가 그 자체의 표현형을 유지하고 있음을 보여 주고 있다. 그 외 apoptosis를 유발시키는데 관여하는 Dkk-1와 Nip3는 실험군에서 높게 발현되었고, apoptosis를 억제시키는데 관여하는 Btf와 TAX1BP1는 오히려 낮게 발현됨을 알 수 있으므로 이는 실험군에서 치주인대세포가 골아세포로의 분화되었음을 나타낸다.

• 생태와환경
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• 제34권4호통권96호
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• pp.310-318
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• 2001

낙동강에서 치어섭식이 동물플랑크톤에 미치는 영향을 채집된 치어의 소화관조사를 통해 고찰하였다. 조사지역인 낙동강 하류 물금에서는 유량이 감소하고 수체가 정체되는 5월에서 6월사이 5종류의 치어가 높은 밀도를 나타내었다 같은 시기, 동물플랑크톤 군집내에서는 대형윤충류인 Asplanchna spp.와 Brachionus spp., 지각류인 Moina micrura 및 Bosminopsis deitersi의 밀도가 큰 폭으로 감소하였다. 반면, Polyarthra속의 소형윤충류는 최대 밀도를 나타내었다. 채집된 치어의소화관 조사 결과, 15 mm이하의 치어는 대형윤충류 및 지각류를, 15mm 이상의 치어인 경우 지각류를 선택적으로 섭식하는 것으로 나타났다. 큰입배스의 치어의 경우, 같은 시기 채집된 치어에 비해 대형이었으며 동물플랑크톤 뿐 아니라 다른 치어를 포식하는 것으로 조사되었다. 조사 결과, 이시기의 낙동강 동물플랑크톤 군집내의 대형 윤충류와 지각류의 급격한 밀도 감소는 치어의 섭식으로 인한 것으로 사료된다. 반면, 이러한 밀도감소는 치어의 수가 감소함에 따라 회복되는 경향을 보였다. 부영양화된 낙동강의 경우 배스치어의 섭식으로 인한 치어밀도의 급속한 감소, 풍부한 먹이농도 등이 감소한 지각류의 밀도회복을 유도하는 것으로 사료된다.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2005년도 춘계 학술발표회 논문집
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• pp.209-216
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• 2005

The controller that can control the smart base isolation system consisting of M damper and friction pendulum systems(FPS) is developed in this study. A fuzzy logic controller (FLC) is used to modulate the M damper force because the FLC has an inherent robustness and ability to handle non-linearities and uncertainties. A genetic algorithm (GA) is used for optimization of the FLC. When earthquake excitations are applied to the structures equipped with smart base isolation system, the relative displacement at the isolation level as well as the acceleration of the structure should be regulated under appropriate level. Thus, NSGA-II(Non-dominated Sorting Genetic Algorithm) is employed in this study as a multi-objective genetic algorithm to meet more than two control objectives, simultaneously. NSGA-II is used to determine appropriate fuzzy control rules as well to adjust parameters of the membership functions. Effectiveness of the proposed method for optimal design of the FLC is judged based on computed responses to several historical earthquakes. It has been shown that the proposed method can efficiently find Pareto optimal sets that can reduce both structural acceleration and base drift from numerical studies.

• The Korean Journal of Physiology and Pharmacology
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• 제24권1호
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• pp.121-126
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• 2020

The ezrin-radixin-moesin (ERM) proteins are a family of membrane-associated proteins known to play roles in cell-shape determination as well as in signaling pathways. We have previously shown that amphetamine decreases phosphorylation levels of these proteins in the nucleus accumbens (NAcc), an important neuronal substrate mediating rewarding effects of drugs of abuse. In the present study, we further examined what molecular pathways may be involved in this process. By direct microinjection of LY294002, a PI3 kinase inhibitor, or of S9 peptide, a proposed GSK3β activator, into the NAcc core, we found that phosphorylation levels of ERM as well as of GSK3β in this site are simultaneously decreased. These results indicate that ERM proteins are under the regulation of Akt-GSK3β signaling pathway in the NAcc core. The present findings have a significant implication to a novel signal pathway possibly leading to structural plasticity in relation with drug addiction.

• 한국화재소방학회논문지
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• 제16권2호
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• pp.51-58
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• 2002

몬트리올 의정서에 의해서 규제받는 Halon-1301과 대체 물질중의 하나인 $CO_2$의 물성(밀도, 점도, 엔탈피)의 실험식을 구하였다. 문헌에서 얻은 실험값을 이용하여 온도에 대한 회귀분석에 의해서 실험식을 얻었다. 압축인자를 이용하여 온도에 대한 2차 함수로서 밀도에 관한 실험식을 제안하였다. 점도는 온도에 대한 지수함수로 표시되며, 보정인자를 사용하여 사용 온도범위를 확장하였다. 엔탈피는 열용량과 마찬가지로 온도에 대한 2차 함수로 정의하여 나타내었다. 본 연구에서 제안된 위의 실험식의 상관계수는 0.99 이상이었다.

• 경영과학
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• 제32권1호
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• pp.15-33
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• 2015

Under complete information, introducing additional constraints to a portfolio will have a negative impact on performance. However, real-life investments inevitably involve use of error-prone estimations, such as expected stock returns. In addition to the reality of incomplete data, investments of most Korean domestic equity funds are regulated externally by the government, as well as internally, resulting in limited maximum investment allocation to single stocks and risk free assets. This paper presents an investment framework, which takes such real-life situations into account, based on a newly developed portfolio selection model considering realistic constraints under incomplete information. Additionally, we examined the effects of additional constraints on portfolio's performance under incomplete information, taking the well-known Samsung and SK group stocks as performance benchmarks during the period beginning from the launch of each commercial fund, 2005 and 2007 respectively, up to 2013. The empirical study shows that an investment model, built under incomplete information with additional constraints, outperformed a model built without any constraints, and benchmarks, in terms of rate of return, standard deviation of returns, and Sharpe ratio.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.1001-1007
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• 2003

Metal ions contamination may inhibit microorganisms involved in the biodegradation of organic compounds and affect biodegradation rates. Therefore, it is likely that bioremediation of xenobiotics-contaminated soils and waste will require inoculation with efficient biodegrading microbial communities, with capabilities of being resistant to heavy metals as well. Two different transconjugants (Pseudomonas sp. KMl2TC and P. aeruginosa TC) were constructed by conjugation experiments. Results on MIC, induction and growth inhibition strongly indicated that arsenic-resistant plasmid, pKM20, could be mobilized, and the newly acquired phenotype of pKM20 was not only expressed but also well regulated, resulting in newly acquired resistances to $As^{5+},\;As^{3+},\;and\;Sb^{3+} in\;addition\;to\;Cd^{2+},\;Zn^{2+},\;and\;Hg^{2+}$. The phenol- degradation efficiencies of Pseudomonas sp. KMl2TC were maintained significantly even at high heavy metal concentrations at which these efficiencies of P. aeruginosa TC were completely impaired. The results in this study on the effects of heavy metals on phenol degradation, especially after conjugation, are the first ever reported. All the results described in this study encourage to establish a goal of making "designer biocatalysts" which could degrade certain xenobiotics in the area contaminated with multiple heavy metals.

• 한국환경성돌연변이발암원학회지
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• 제25권2호
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• pp.66-70
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• 2005

Telomerase, a specialized RNA-directed DNA polymerase that extends telomeres of eukaryotic chromosomes, has activity in most malignant tumors and provides a mechanism for the unlimited potential for division of neoplastic cells. Although telomerase is known to be a regulated enzyme, the factors and mechanisms involved in telomerase regulation are not well understood. In the present study, we compared the effect of 12-O­tetradecanoyl-phorbol-13-acetate (TPA) and non-phorbol ester tumor promoters such as okadaic acid, anthralin and benzoyl peroxide on the expression of telomerase in the mouse skin carcinogenesis system, a well characterized model for studying pre-malignant and malignant progression. We found that most early papillomas harvested after 10 weeks of TPA promotion showed telomerase activity. Other papillomas harvested after 10 weeks of okadaic acid, anthralin and benzoyl peroxide promotion and after single treatment of DMBA only also showed telomerase activity, respectively. On the other hand, normal and all skins surrounded by papillomas harvested after 10 weeks of these promoters has no telomerase activity. Taken together these results, there appears to be no clear association between the level of telomerase activity and protein phosphorylation in mouse skin papillomas and telomerase may be useful as bio-markers in early detection of tumors.

• KIEAE Journal
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• 제5권2호
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• pp.19-26
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• 2005

Every site has a different given geometrical and climatic condition, which influenced not only the lifestyle of the humanbeings but also the regional architecture. For example, on a cold region, the reduction of the energy loss is necessary, like an igloo, which has a littlest energy loss at hemisphere. Or on a warm region, the house must be protected thermally from the overheating at the sunshining. like a huge shading. An architectural interpretation in the (extreme) moderate climate, like Korea, has always tried to satisfy the both opposite demands simultaneously. A facade, which divides out- and inside, has an ideal position to lead the regulated regional climatic conditions into the room. The Double Facade System(DFS) is well known as an innovative solution in the european countries, like Germany. It provides an reasonable alternative, which can achieve these goals at the same time. A Double Facade System provides an effective sunshade, which means a cooling energy reduction at the warm season. In addition, it enables a natural ventilation at the cold season with the preheating at the system as well as spring and autumn. An ordinary Single Facade System with a inside or outside sun blind provides a solution just for a specified season, like a summer or winter. But the Double Facade System can deal with the various climatic conditions in the moderate climate.