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Optimization of Antimicrobial Activity Against Food-borne Pathogens in Grapefruit Seed Extract and a Lactic Acid Mixture (식품위해미생물에 대한 자몽종자 추출물과 젖산 혼합물의 항균효과 최적화)

  • Kim, Hae-Seop;Park, Jeong-Wook;Park, In-Bae;Lee, Young-Jae;Kim, Jeong-Mok;Jo, Yeong-Cheol
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.472-481
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    • 2009
  • Response surface methodology (RSM) is frequently used for optimization studies. In the present work, RSM was used to determine the antimicrobial activitiesof grapefruit seed extract (GFSE) and a lactic acid mixture (LA) against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella typhimurium, Pseudomonas fluorescens, and Vibrio parahaemolyticus. A central composite design was used to investigate the effects of independent variables on dependent parameters. One set of antimicrobial preparations included mixtures of 1% (w/w) GFSE and 10% (w/w) LA, in which the relative proportions of component antimicrobials varied between 0 and 100%. In further experiments, the relative proportions were between 20% and 100%. Antimicrobial effects against various microorganisms were mathematically encoded for analysis. The codes are given in parentheses after the bacterial names, and were S. aureus ($Y_1$), B. cereus ($Y_2$), E. coli ($Y_3$), S. typhimurium ($Y_4$), P. fluorescens ($Y_5$), and V. parahaemolyticus ($Y_6$). The optimum antimicrobial activity of the 1% (w/w) GFSE:10% (w/w) LA mixture against each microorganism was obtained by superimposing contour plots ofantimicrobial activities on measures of response obtained under various conditions. The optimum rangesfor maximum antimicrobial activity of a mixture with a ratio of 1:10 (by weight) GFSE and LA were 35.73:64.27 and 56.58:43.42 (v/v), and the optimum mixture ratio was 51.70-100%. Under the tested conditions (a ratio of 1% [w/w] GFSE to 10% [w/w] LA of 40:60, and a concentration of 1% [w/w] GFSE and 10% [w/w] LA, 70% of the highest value tested), and within optimum antimicrobial activity ranges, the antimicrobial activities of the 1% (w/w) GFSE:10% (w/w) LA mixture against S. aureus ($Y_1$), B. cereus ($Y_2$), E. coli ($Y_3$), S. typhimurium ($Y_4$), P. fluorescens ($Y_5$), and V. parahaemolyticus ($Y_6$) were 24.55, 25.22, 20.20, 22.49, 23.89, and 28.04 mm, respectively. The predicted values at optimum conditions were similar to experimental values.

Influence of Cultivated Regions in Organic and Conventional Farming Paddy Field (벼 유기농업과 관행농업에 미치는 재배지역의 영향)

  • Lee, Seong-Tae;Seo, Dong-Cheol;Cho, Ju-Sik;Kim, Eun-Seok;Song, Won-Doo;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.408-414
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    • 2011
  • The purpose of this study was to find out optimum cultivated regions for rice organic farming. The organic and conventional rice as control were grown in three different places : secluded hill paddy field for Hapcheon, normal rural paddy field for Sancheong, and suburban paddy field for Jinju from 2005 to 2006. In secluded hill paddy field, the organic material and pesticide to control pest and disease were input twice for organic and conventional rice cultivation. However, in normal rural and suburban paddy field, those were input three times for organic and conventional rice cultivation. The occurrence of sheath blight in organic farming was higher than in conventional farming. Whereas brown planthopper population per 20 plant was significantly high 10.1~19.5 for conventional farming compared with 4.4~10.0 for organic farming. For that reason, the density of the brown planthoppers was higher in organic farming than those in conventional farming. Dominated weeds occurred in organic and conventional paddy field were namely Monochoria vaginalis, Ludwigia prostrata, and Cyperus difformis. The population per 20 plant and dried weight per $m^2$ of weeds were higher in 121 and 50.5 g for organic paddy field. The productivity of rice in different cultivated regions for organic farming was $2.96Mg\;ha^{-1}$ in hill paddy field, $4.03Mg\;ha^{-1}$ in normal rural and suburban paddy field. Toyo-taste value and ratio of perfect grain of milled rice were not different by cultivated regions in both farming system.

Effect of 10 Weeks Smart Machine Circulation Exercise on Body Composition, Lung Function, Blood Lipids and Insulin Resistance in Obesity Middle-aged Women (10주간 스마트머신 순환운동이 비만 중년여성의 체조성, 폐기능, 혈중지질 및 인슐린 저항성에 미치는 영향)

  • Kim, Min-Chan;Ha, Soo-Min;Koh, Su-Han;Kim, Jong-Won;Kim, Do-Yeon
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.4
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    • pp.951-962
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    • 2021
  • The purpose of this study was to analyze the effects of smart machine circulation exercise on body composition, lung function, blood lipid and insulin resistance in obesity middle-aged women among 40-60 years by dividing them into a smart machine circulation exercise group(n=8), and control group(n=6). The smart machine circulation exercise program included 55-minutes sessions thrice each week at the following intensities: The strength of aerobic exercise is applied to smart machines by linking the smart machine with the POLAR T31; the 1-4 week is 40-50%HRR, 5-8 week is 50-60%HRR, and 9-10 week is 60-70%HRR. The strength of the resistance exercise was tested using a smart machine based on the constant velocity motion, and then, using the 1-RM data value, applied 40% 1-RM for 1-4 weeks, 60% 1-RM for 5-8 weeks, and 80% 1-RM for 9-10 week. As a results, body composition indicated that weight, BMI, %BF, WHR had a significant interaction effect. Lung function indicated that FVC levels significantly changes in the exercise group and the between groups difference in changes at 10week was significant. Also, FVC and FEV1 significantly showed interaction effect. TC, TG and HDL-C levels significantly changes in smart machine circulation exercise group and the between-group difference in changes after 10 weeks was significant. TC, TG and HDL-C significantly showed interaction effect. Insulin resistance demonstrated that Insulin, Glucose and HOMA-IR levels significantly showed difference over 10 weeks between group. Therefore, the 10 weeks smart machine circulation exercise positively effects on the body composition, lung function, blood lipids, and insulin resistance in obesity middle-aged women and this smart machine circulation exercise can improve their obesity and prevent obesity.

Assessment Selective Breeding Effect of Israeli carp (Cyprinus carpio) from Korea (국내 이스라엘 잉어의 선발육종효과 평가)

  • Kim, Jung Eun;Hwang, Ju-ae;Kim, Hyeong Su;Im, Jae Hyun;Lee, Jeong-Ho
    • Korean Journal of Ichthyology
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    • v.32 no.4
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    • pp.210-221
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    • 2020
  • Since the introduction of Israeli carp into Korea for farming in 1973, there are no breeding studies on developing Korea Israeli carp (domestic) so far. This study performed gene-based cross-breeding studies to restore genetic diversity of lowered Israeli carp through continuous inbreeding, and for rapid growth and better scales. This study produced four cross-breeding groups (F1) using Koean Israeli carp and Chinese Songpu mirror carp for the improvement of growth and scale of Israeli carp in Korea. And mating scheme for breeding groups was set in consideration of the morphological analysis and genetic distance of broodstock. In addition, this study used microsatellite markers and genotype data to analyze genetic diversity and parentage analysis. As a result, the average NA and HE values of Korean select broodstock are 8.3 and 0.743, and F1 is 13.0 and 0.764. This study shows that the genetic diversity of F1 has been recovered over Korean Israeli carp through breeding between Korean Israeli carp and Chinese Songpu mirror carp. Common Israeli carp in Korea reached 1.7 kg in 17 months, and improved Israeli carp reached to 2.2 kg. The KC (Korea×China, KC) group was 2.52 and broodstock group was 3.15. F1 showed lower scale score (0.63) than broodstock. The improved carp (F1; CK, KC) had 20% better scales than the parent group (F0), which improved 27% in weight and 25% in scales compared to common Israeli carp. The Israeli carp developed by the genetics-based breeding grew quicker and had improved genetic diversity and fewer scales, which will be of great value for Korean Israeli aquaculture industry due to good marketability.

Effects of Chlorine Water and Plasma Gas Treatments on the Quality and Microbial Control of Latuca indica L. Baby Leaf Vegetable during MA Storage (염소수와 플라즈마 가스 처리가 왕고들빼기 어린잎채소의 MA저장 중 품질과 미생물 제어에 미치는 영향)

  • Kim, Ju Young;Han, Su Jeong;Whang, Lixia;Lee, Joo Hwan;Choi, In-Lee;Kang, Ho-Min
    • Journal of Bio-Environment Control
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    • v.28 no.3
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    • pp.197-203
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    • 2019
  • This study was carried out to investigate the effect of chlorine water and plasma gas treatment on the quality and microbial control of Latuca indica L. baby Leaf during storage. Latuca indica L. baby leaves were harvested from a plant height of 10cm. They were sterilized with $100{\mu}L{\cdot}L^{-1}$ chlorine water and plasma-gas (1, 3, and 6hours), and packaged with $1,300cc{\cdot}m^{-2}{\cdot}day^{-1}{\cdot}atm^{-1}$ films and then stored at $8{\pm}1^{\circ}C$ and RH $85{\pm}5%$ for 25days. During storage, the fresh weight loss of all treatments were less than 1.0%, and the carbon dioxide and oxygen concentrations in packages were 6-8% and 16-17%, respectively for all treatments in the final storage day. The concentration of ethylene in the packages fluctuated between $1-3{\mu}L{\cdot}L^{-1}$ during the storage and the highest concentration of ethylene was observed at 6 hours plasma treatment in the final storage day. The off-odor of all treatments were almost odorless, the treatments of chlorine water and 1 hour plasma maintained the marketable visual quality until the end of storage. Chlorophyll content and Hue angle value measured at the final storage day were similar to those measured before storage in chlorine water and 1 hour of plasma treatments. E. coli was not detected immediately after sterilization in all sterilization treatments. After 6 hours of plasma treatment, the total bacteria fungus counts were lower than the domestic microbial standard for agricultural product in all sterilization treatments. The total aerobic counts in the end storage day increased compared to before storage, whereas E. coli was not detected in all sterilization treatments. The sterilization effect against bacteria and fungi was the best in chlorine water treatment. Plasma treatment showed sterilization effects, but within a prolonged period of time. In addition, the sterilization effect decreased gradually. These results suggest that chlorine water and plasma treatment were effective in maintaining Latuca indica L. baby Leaf commerciality and controlling microorganisms during postharvest storage.

How the Information Location (up vs. down) Impacts Promotion Attractiveness and Amount Perception (판매촉진 정보의 위치(위vs.아래)가 판매촉진 매력도 및 증정량 지각에 미치는 영향)

  • Jang, JungMin;Kim, Yeong-Ju
    • (The) Korean Journal of Advertising
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    • v.27 no.3
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    • pp.7-25
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    • 2016
  • According to recent studies, the location of visual information has a significant impact on consumers' interpretation and judgment in various contexts. In particular, vertical positioning is relevant to the perception of weight: consumers tend to interpret that products are heavier when information is placed at the bottom of a page or section rather than the top. This is due to the common perception that heavy things would sink to the bottom and light things would float up. Consistent with this research stream, the current study investigates how the vertical location of freebie information influences promotion evaluation. We hypothesize that consumers assume that the complimentary product is heavier when the information about it is displayed at the bottom compared to when the information is displayed at the top. Two experiments were conducted. In experiment 1, we show how the location of "get one free" information impacts the evaluation of the sales promotion in a "Buy two and get one free" context. When the complimentary information was displayed below rather than above the main product information, consumers evaluated the sales promotion more favorably. Experiment 2 tests the generalizability of the vertical location effect by using a different type of information and product: a visual picture of vitamin tablets rather than the numeric information as in Experiment 1. The dependent variable was also extended to the perceived amount of the promoted product. We suggest the boundary condition that the proposed effect is moderated by the presence or absence of an explicit message about the amount. Without an explicit message about the amount of the product, the results were similar to those of experiment 1 in that consumers perceived that the product offered a greater amount when the complimentary information was displayed at the bottom rather than at the top. However, the location effect disappeared with an explicit message about the amount. This confirms the moderating effect that presenting an explicit message about the amount is a crucial boundary condition for location effect in a value added promotion. Marketers can use this knowledge to formulate strategies in a variety of sales promotion conditions.

Conservation of Removing Surface Contaminants on Silla monument at Jungsung-ri using Nd:YAG Laser Cleaning System (Nd/YAG레이저를 이용한 포항중성리신라비 표면오염물 제거와 보존처리)

  • Lee, Tae Jong;Kim, Sa Dug;Lee, Joo Wan;Oh, Jung Hyeon;Lee, Myeong Seong
    • Korean Journal of Heritage: History & Science
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    • v.44 no.4
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    • pp.142-153
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    • 2011
  • 'Silla Monument Stone in Jungseong-ri, Pohang' was discovered in Pohang City, Gyeongsangbuk Province of Korea in 2009. The monument stone with irregular shape has dimensions of maximum height of 105cm, width of 47.6~49.6cm, thickness of 13.8~14.7cm and weight of 115kg. The results of monument stone was found to be granitite. Conservation treatment procedure was carried out in the order of production of Weathering map, cleaning of surface pollutants, consolidation using ethyl silicate. Black pollutant(crust) that covered more than 60% of the surface was analyzed first in order to remove the pollutants on the surface of the monumental stone by cleaning of surface pollutants using laser. The purpose on analysis was not only to verify the pollutants on the stone but also to carry out preliminary cleaning by securing rocks with same pollutant as the monumental stone. As the results of analysis using p-XRF(PMI. INNOV-X, USA) on the site, high level of Mn and Fe were detected, and the analysis of small section that had been fallen off with SEM/EDX for the purpose of quantitative analysis also detected high level of Mn. The Similar contaminants on Stone secured in the manner described above were made into 10 samples ($5cm{\times}5cm$) and was subjected to preliminary cleaning by Nd-YAG Laser(Laser&Physics, Korea). The results of surface observation through portable microscope during cleaning revealed that the power of 460mJ, wavelength of 1064nm and irradiation frequency of 1,800~2,300 per $25cm^2$ were most effective. Evaluation on the preservative treatment was made through confirmation of the extent of removal through color-difference meter measurement and component analysis prior to and following removal of the pollutants. As the result of color-difference meter measurement increase in the brightness was evidenced by the increase in the brightness ($L^*$)value from 33 to 49, and it was possible to ascertain the reduction in the pollutants as the content of Mn was reduced by about 80% from $50,000{\pm}5,000ppm$ to $10,000{\pm}2,000pmm$ from the result of component analysis.

Comparison of Agricultural Traits and Physicochemical Properties of Lentil (Lens culinaris Med.), Chickpea (Cicer aretinum L.), and Guar (Cyamopsis tetragonoloba L.) Germplasms Collected from Tropical and Subtropical Regions (열대, 아열대 지역 수집 렌즈콩, 병아리콩, 송이콩 유전자원의 농업형질과 이화학적 특성 비교)

  • Choi, Yu-Mi;Lee, Sukyeung;Lee, Myung-Chul;Oh, Sejong;Hur, Onsook;Cho, Gyu Taek;Yoon, Munsup;Hyun, Do Yoon
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.453-462
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    • 2018
  • This study was carried out to investigate the utilization value of legume crops collected in tropical and subtropical areas. We examined agronomic traits to assess domestic adaptability and evaluated useful components of foreign legumes. We used a total of 201 genetic resources of three legumes, consisting of 68 lentils, 72 chickpeas and 61 guars. The average number of days to flowering of the three legumes ranged from 56.7 to 60.8 days; the shortest in guar and longest in chickpea. The average number of days to growth of the three legumes ranged from the shortest 86.8 days in lentil, to the longest 163.9 days in guar. The maturation period of the three legumes lasted from the end of May until mid-September, based on sowing in March. However, the average yield of lentil was very low, ranging from 0.5 g to 30.6 g, with an average 16.4 g based on 10 plants per accession. The average 100 seed weight of the three legumes was 2.2 g for lentil, 22.9 g for chickpea, and 3.8 g for guar. The crude protein content ranged from 14.1% to 32.4% with an average of 20.4%, the highest for guar and the lowest for chickpea. The average crude oil content in the three legume crops was generally low, ranging from 0.8% in lentil, to 4.3% in chickpea. The average dietary fiber content in the three legume crops varied from 15.7% to 50.7%. Guar was the highest source of fiber, followed by chickpea (19.3%) and lentil (15.7%). From the agricultural traits analysis, chickpea and guar could grow domestically. However, lentil was difficult to flower and fruit normally during the warmer season after May. Therefore, lentil should be considered for late summer cropping during the cool season. The physicochemical properties of the three legumes seem to be useful as they are similar to, or better than, those of the control common bean.

A Study on the Possibility of Recycling Coir Organic Substrates for using Strawberry Hydroponics Media (토마토 폐배지를 딸기 수경재배 배지로 재이용 가능성 연구)

  • Lee, Gyu-Bin;Park, Young-Hoon;Choi, Young-Whan;Son, Beung-Gu;Kim, Jooh-Yup;Kang, Nam-Jun;Kang, Jum-Soon
    • Journal of Korea Society of Waste Management
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    • v.34 no.2
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    • pp.205-213
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    • 2017
  • The current study was performed to investigate the effect of recycling coir substrates on the growth, fruit yield, and quality of strawberry plants. Analysis of physical properties revealed that the pH of a fresh coir substrate was 5.04 while those of substrates reused for one and two years were 5.20 and 5.33, respectively. The electrical conductivity (EC) of a new substrate was as high as $4.58dS{\cdot}m^{-1}$. This can cause salt stress after transplanting. The EC tended to decrease as the substrate was recycled, and the EC of a two-year recycled substrate was $1.48dS{\cdot}m^{-1}$. The fresh substrate had lower nitrogen and calcium concentrations, but higher phosphate, potassium, and sodium concentrations than the recycled coir substrate. The coir substrates recycled for one or two years maintained better chemical properties for plant growth than the fresh substrate. Strawberry growth varied depending on the number of years that the coir substrate was recycled. In general, strawberries grown in substrates that had been reused for two years did better than those grown in substrates that had been reused once or were fresh. Ninety days after transplanting, a plant grown in a substrate that had been reused for two years contained 25 leaves, which was 3.6 more than with a fresh substrate. In addition, the plants grown in a substrate that had been reused for two years exhibited larger leaf areas than those grown in other substrates. Coir substrates that had been reused for one year increased the number and area of leaves, but not as much as the substrate that had been reused for two years. One- and two-year reused coir substrates increased the weight of strawberries produced relative to the unused substrate, but the difference was not statistically significant. The plants grown in two-year reused substrates were longer and wider, as well. Also, the number of fruits per plant was higher when substrates were reused. Specifically, the number of fruits per plant was 28.7 with a two-year reused substrate, but only 22.2 with a fresh substrate. The fruit color indices (as represented by their Hunter L, a, b values) were not considerably affected by recycling of the coir substrate. The Hunter L value, which indicates the brightness of the fruit, did not change significantly when the substrate was recycled. Neither Hunter a (red) nor b (yellow) values were changed by recycling. In addition, there were no significant changes in the hardnesses, acidities, or soluble solid-acid ratios of fruits grown in recycled substrates. Thus, it is thought that recycling the coir substrate does not affect measures of fruit quality such as color, hardness, and sugar content. Overall, reuse of coir substrates from hydroponic culture as high-bed strawberry growth substrates would solve the problems of new substrate costs and the disposal of substrates that had been used once.

Development of water quality and aquatic ecosystem model for Andong lake using SWAT-WET (SWAT-WET을 이용한 안동호의 수질 및 수생태계 모델 구축)

  • Woo, Soyoung;Kim, Yongwon;Kim, Wonjin;Kim, Sehoon;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.54 no.9
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    • pp.719-730
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    • 2021
  • The objective of this study is to develop the water quality and aquatic ecosystem model for Andong lake using SWAT-WET (Soil and Water Assessment Tool-Water Ecosystem Tool) and to evaluate the applicability of WET. To quantify the pollutants load flowing into Andong lake, a watershed model of SWAT was constructed for Andong Dam basin (1,584 km2). The calibration results for Dam inflow and water quality loads (SS, T-N, T-P) were analyzed that average R2 was more than 0.76, 0.69, 0.84, and 0.60 respectively. The calibrated SWAT results of streamflow and nutrients concentration was used into WET input data. WET was calibrated and validated for water temperature, dissolved oxygen, and water quality concentration (T-N, T-P) of Andong lake. The WET calibrated results was analyzed that PBIAS was +19%, -13%, +4%, and +26.5% respectively and showed that it was simulated to a significant level compared with the observation data. The observed dry weight (gDW/m2) of zoobenthos was less than 0.5, but the average value of simulation was analyzed to be 0.8, which is because the WET model considers zoobenthos with a broader concept. Although accurate calibration is difficult due to the lack of observed data, SWAT-WET can analyze the effects of environmental change in the upstream watershed on the lake based on long-term simulation based on watershed model. Therefore, the results of this study can be used as basic data for managing the aquatic environment of Andong lake.