Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Effects of Chlorine Water and Plasma Gas Treatments on the Quality and Microbial Control of Latuca indica L. Baby Leaf Vegetable during MA Storage

염소수와 플라즈마 가스 처리가 왕고들빼기 어린잎채소의 MA저장 중 품질과 미생물 제어에 미치는 영향

  • Kim, Ju Young (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University) ;
  • Han, Su Jeong (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University) ;
  • Whang, Lixia (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University) ;
  • Lee, Joo Hwan (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University) ;
  • Choi, In-Lee (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University) ;
  • Kang, Ho-Min (Division of Horticulture and Systems Engineering, Program of Horticulture, Kangwon National University)
  • 김주영 (강원대학교 원예.시스템공학부 원예과학전공) ;
  • 한수정 (강원대학교 원예.시스템공학부 원예과학전공) ;
  • 왕립 (강원대학교 원예.시스템공학부 원예과학전공) ;
  • 이주환 (강원대학교 원예.시스템공학부 원예과학전공) ;
  • 최인이 (강원대학교 원예.시스템공학부 원예과학전공) ;
  • 강호민 (강원대학교 원예.시스템공학부 원예과학전공)
  • Received : 2019.03.13
  • Accepted : 2019.05.27
  • Published : 2019.07.30
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Abstract

This study was carried out to investigate the effect of chlorine water and plasma gas treatment on the quality and microbial control of Latuca indica L. baby Leaf during storage. Latuca indica L. baby leaves were harvested from a plant height of 10cm. They were sterilized with $100{\mu}L{\cdot}L^{-1}$ chlorine water and plasma-gas (1, 3, and 6hours), and packaged with $1,300cc{\cdot}m^{-2}{\cdot}day^{-1}{\cdot}atm^{-1}$ films and then stored at $8{\pm}1^{\circ}C$ and RH $85{\pm}5%$ for 25days. During storage, the fresh weight loss of all treatments were less than 1.0%, and the carbon dioxide and oxygen concentrations in packages were 6-8% and 16-17%, respectively for all treatments in the final storage day. The concentration of ethylene in the packages fluctuated between $1-3{\mu}L{\cdot}L^{-1}$ during the storage and the highest concentration of ethylene was observed at 6 hours plasma treatment in the final storage day. The off-odor of all treatments were almost odorless, the treatments of chlorine water and 1 hour plasma maintained the marketable visual quality until the end of storage. Chlorophyll content and Hue angle value measured at the final storage day were similar to those measured before storage in chlorine water and 1 hour of plasma treatments. E. coli was not detected immediately after sterilization in all sterilization treatments. After 6 hours of plasma treatment, the total bacteria fungus counts were lower than the domestic microbial standard for agricultural product in all sterilization treatments. The total aerobic counts in the end storage day increased compared to before storage, whereas E. coli was not detected in all sterilization treatments. The sterilization effect against bacteria and fungi was the best in chlorine water treatment. Plasma treatment showed sterilization effects, but within a prolonged period of time. In addition, the sterilization effect decreased gradually. These results suggest that chlorine water and plasma treatment were effective in maintaining Latuca indica L. baby Leaf commerciality and controlling microorganisms during postharvest storage.

본 연구는 왕고들빼기 어린잎의 염소수 및 플라즈마 처리가 저장 중 품질 및 미생물 제어에 미치는 영향을 알아보고자 수행하였다. 초장이 10cm 수준에서 수확한 왕고들빼기 어린잎을 100ppm의 염소수와 플라즈마 가스로 1, 3, 6시간동안 살균 처리한 후 산소투과도가 $1,300cc{\cdot}m^{-2}{\cdot}day^{-1}{\cdot}atm^{-1}$인 OTR(oxygen transmission rate) 필름을 사용하여 포장하였고, $8{\pm}1^{\circ}C$의 저장 온도와 상대 습도 $85{\pm}5%$의 조건에서 25일간 저장하였다. 저장 중 생체중 감소율은 모든 처리구가 1.0% 미만을 보였고, 저장 종료일 모든 처리구의 포장 내 산소 농도는 16-17%을 보였고, 포장 내 이산화탄소 농도는 6-8%의 수준을 보였다. 포장 내 에틸렌 농도는 저장 기간 중 $1-3{\mu}L{\cdot}L^{-1}$의 수준으로 증감을 반복하였는데, 저장 10일째부터 저장 종료일까지 플라즈마 6시간 처리구가 가장 높은 농도를 보였다. 모든 처리구의 이취는 거의 느껴지지 않는 수준이었고, 염소수 및 플라즈마 가스 1시간 처리가 저장 종료일까지 상품성을 유지하였다. 저장 종료일에 조사한 엽록소 함량과 Hue angle 값은 염소수와 플라즈마 1시간 처리가 저장 전과 유사한 수준으로 높은 수준을 유지하였다. 살균 처리 직후 모든 살균 처리구에서 대장균은 검출되지 않았고, 총 세균 및 총 곰팡이 수는 플라즈마 6시간 처리구를 제외한 모든 살균 처리구에서 국내 미생물 허용 기준을 충족하였다. 저장 종료일 조사한 총 미생물수는 저장 전에 비해 증가하였으나 대장균은 모든 살균 처리구에서 검출되지 않았다. 세균과 곰팡이에 대한 살균효과는 염소수 처리가 가장 우수하였고, 플라즈마 처리구는 살균효과는 나타났으나 처리 시간이 길어짐에 따라 그 효과는 미비하였다. 위의 결과를 종합해보면, 왕고들빼기 어린잎은 염소수 처리 및 단시간 플라즈마 처리 시 황화 및 부패 억제를 통한 상품성 유지 및 미생물 제어에 효과가 있는 것으로 판단된다.

Keywords

References

  1. Bae, Y.S., H.J. Choi, J.H. Lee, and J.W. Choi. 2017. Influence of ozone produced by non-thermal plasma on the inhibition of conidia germination of Botrytis cinerea and Alternaria sp. J. Kor. Hort. Sci. Technol. 35:223(Abstr.).
  2. Baur, S., R. Klaiber, W.P. Hammes, and R. Carle. 2004. Sensory and microbiological quality of shredded, packaged iceberg lettuce as affected by pre-washing procedures with chlorinated and ozonated water. Inno Food Sci Emerg Technol. 5:45-55. https://doi.org/10.1016/j.ifset.2003.10.002
  3. Chang, T.E., S.Y. Moon, K.W. Lee, J.M. Park, J.S. Han, O.J. Song, and I.S. Shin. 2004. Microflora of manufacturing process and final products of saengshik. J. Kor. Hort. Sci. Technol. 36:501-506.
  4. Choi, S.T., S.Y. Oh, J.E. Lee, J.G. Kim, H.E. Lee, and C.I. Lim. 2004. Effect of washing treatments on food safety of leafy vegetables. J. Kor. Hort. Sci. Technol. 22: 68(Abstr.).
  5. Choi. K.Y., S.H. Kim, J.K. Kim, H.J. Yoo, and I.S. Kim. 2016. Effect of light control on growth of baby leaf vegetables using rice seedling tray. Journal of Agricultural, Life and Environmental Sciences. 28:56-62.
  6. Critzer, F.J., K. Kelly-Wintenberg, S.L. South, and D.A. Golden. 2007. Atmospheric plasma inactivation of foodborne pathogens on fresh produce surfaces. J. Food Prot. 70:2290-2296. https://doi.org/10.4315/0362-028X-70.10.2290
  7. Hwang, T.Y. 2017. Effect of commercial sanitizers on microbial quality of fresh-cut iceberg lettuce during storage. Korean J. Food Preserv. 24:827-833. https://doi.org/10.11002/kjfp.2017.24.6.827
  8. Kabir, H. 1994. Fresh-cut vegetables. In: Modified atmosphere food packaging.
  9. Kays, S.J. and E.R. Paull. 2004. Postharvest biology. Exon Press, Athens, GA, USA.
  10. Kim, J.E., D.U. Lee, and S.C. Min. 2014. Microbial decontamination of red pepper powder by cold plasma. Food Microbiol. 38:128-136. https://doi.org/10.1016/j.fm.2013.08.019
  11. Kim, J.E., I.H. Kim, and S.C. Min. 2013. Microbial decontamination of vegetables and spices using cold plasma treatments. J. Kor. Food Sci. Technol. 45:735-741. https://doi.org/10.9721/KJFST.2013.45.6.735
  12. Kim, J.Y., S.J. Han, I.L. Choi, J.S. Yoon, Y.H. Moon, S.M. Kim, and H.M. Kang. 2018. Effects of OTR film type on the quality of Lepidium sativum L. baby leaf vegetable during MA storage. Protected Horticulture and Plant Factory, 27:180-184. https://doi.org/10.12791/KSBEC.2018.27.2.180
  13. Kim, H.J., H.J. Song, and K.B. Song. 2011a. Effect of combined treatment of aqueous chlorine dioxide with ultraviolet-c on the quality of red chicory and pak choi during storage. J. Kor. Soc. Food Sci. Nutr. 40:245-252. https://doi.org/10.3746/jkfn.2011.40.2.245
  14. Kim, H.Y, and Y.H. Lee. 2009. A study on the microbiological quality of vegetables in relation to the sanitization method used and vegetable types. J. Kor. Food Cookery Sci. 25:632-642.
  15. Kim, W.I., M.G. Gwak, A.R. Jo, S.D. Ryu, S.R. Kim, S.H. Ryu1, H.Y. Kim, and J.G. Ryu. 2017. Investigation of Microbiological Safety of on-farm Produce in Korea. J. Food Hygiene and Safety. 32:20-26. https://doi.org/10.13103/JFHS.2017.32.1.20
  16. Kim, K.S., S.J. Yoo, K.H. Lee, J.W. Kim, C.B. Park, D.G. Song, and K.S. Jin. Oct. 28, 2009. Sterilizing method using cold plasma. Korea Patent Application No. KR20090112364A.
  17. Kim, S.R., J.Y. Lee, S.H. Lee, W.L. Kim, K.H. Park, H.J. Yun, B.S. Kim, D.H. Chung, J.C. Yun, and K.Y. Ryu. 2011b. Evaluation of microbiological safety of lettuce and cultivation area. J. Fd Hyg. Safety. 26:289-295.
  18. Kwon, K.H., J.M. Sung, J.Y. Kim, B.S. Kim, and S.H. Kim. 2017. Quality characteristics of beef in thermoelectric cooling system combined with plasma during storage. J. Kor. Food Preserv. 24:52-59. https://doi.org/10.11002/kjfp.2017.24.1.52
  19. Lee, K.A., Y.A. Lee, and I.S. Park. 2009. Sanitation effect of sprouts by chlorine Water. Journal of Life Science. 19:751-755. https://doi.org/10.5352/JLS.2009.19.6.751
  20. Lee, H.O., Y.J. Lee, J.Y. Kim, and B.S. Kim. 2018. Effect of combined pallet unit MAP and plasma treatment for extending the freshness of spring kimchi cabbage. J. Kor. Hort. Sci. Technol. 36:224-236.
  21. Loaiza, J. and M. Cantwell. 1997. Postharvest physiology and quality of cilantro (Coriandrum sativum L.). HortScience 32:104-107. https://doi.org/10.21273/HORTSCI.32.1.104
  22. McGuire, R.G. 1992. Reporting of objective color measurements. HortScience 27:1254-1255. https://doi.org/10.21273/HORTSCI.27.12.1254
  23. Mehmet, K. and Y.S. Ilkin. 2007. Antimicrobial effect of koruk (unripe grape-Vitis vinifera) juice against Salmonella typhimurium on salad vegetables. Food Control. 18:702-706. https://doi.org/10.1016/j.foodcont.2006.03.004
  24. Misra, N.N, K.M. Keener, P. Bourke, J.P. Mosnier, and P.J. Cullen. 2014. In-package atmospheric pressure cold plasma treatment of cherry tomatoes. Journal of Bioscience and Bioengineering. 118:177-182. https://doi.org/10.1016/j.jbiosc.2014.02.005
  25. Moreau, M., N. Orange, and M. Feuilloley. 2008. Non-thermal plasma technologies: new tools for bio-decontamination. Biotechnol. Advances. 26:610-617. https://doi.org/10.1016/j.biotechadv.2008.08.001
  26. Park, H.O., C.M. Kim, G.J. Woo, S.H. Park, D.H. Lee, E.J. Chang, and K.H. Park. 2001. Monitoring and trends analysis of food poisoning outbreaks occurred in recent years in korea. Journal of Food Hygiene and Safety. 16:280-294.
  27. Pasquali, F., A.C. Stratakos, A. Koidis, A. Berardinelli, C. Cevoli, L. Ragni, R. Mancusi, G. Manfreda, and M. Trevisani. 2016. Atmospheric cold plasma process for vegetable leaf decontamination: A feasibility study on radicchio (red chicory, Cichorium intybus L.). Food Control. 60:552-559. https://doi.org/10.1016/j.foodcont.2015.08.043
  28. Ragaert. P., W. Verbeke, F. Devlieghere, and J. Debevere. 2004. Consumer perception and choice of minimally processed vegetables and packaged fruits. Food Quality and Prefer. 15:259-270. https://doi.org/10.1016/S0950-3293(03)00066-1
  29. Solberg, M., J.J. Buckalew, C.C. Chen, D.W. Schaffner, K. O'Neil, J. Mcdowell, L.S. Post, and M. Boderck. 1990. Microbiological safety assurance system for foodservice facilities. Food Technol. 44(12):68-73.
  30. Stratakos, A.C, and A. Koidis. 2015. Suitability, efficiency and microbiological safety of novel physical technologies for the processing of ready to eat meals, meats and pumpable products. Int. J. Food Sci. Technol. 50:1283-1302. https://doi.org/10.1111/ijfs.12781
  31. Terada, N., A. Sanada, H. Gemma, and K. Koshio. 2017. Effect of TRANS-2-hecenal vapor pretreatment on alleviattion of heat shock in tomato seedlings (Micro tom). J. ISSAAS. 23:1-7.
  32. Torriani, S. and S. Massa. 1994. Bacteriological survey on ready-to-use sliced carrots. Lebensm-Wiss. Technol. 27:487-490. https://doi.org/10.1006/fstl.1994.1097
  33. Wills, R.B.H. and Warton, M.A., 2000. A new rating scale for ethylene action on postharvest fruit and vegetables. In:Artes, F., Gil, M.I., Conesa, M.A. (Eds.), Improving Postharvest Technologies of Fruits, Vegetables and Ornamentals. Institute International of Refrigeration, Murcia, Spain.
  34. Yoon. G.A. and C.Y. Mok. 2015. Microbial inactivation of grains used in saengshik by corona discharge plasma jet. J. Kor. Food Sci. Technol. 47:70-74. https://doi.org/10.9721/KJFST.2015.47.1.70
  35. Yoon, H.S., I.L. Choi, H.M. Kang. 2017. Different oxygen transmission rate packing films during modified atmosphere storage: Effects on Asparagus Spear Quality. Horticultural Science and Technology 35:314-322. https://doi.org/10.7235/HORT.20170034