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Pathogenesis on enteritis induced by Cryptosporidium parvum alone and combined with porcine rotavirus in piglets (Cryptosporidium parvum 단독 및 돼지 rotavirus와 혼합 감염시킨 자돈 장염의 병원성)

  • Han, Dong-un;Kang, Mun-il;Park, Nam-yong;Wee, Sung-hwan
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.149-158
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    • 1995
  • The purpose of the present study was to understand the pathogenesis of infections in piglets inoculated with C parvum isolated from mice alone and combined with porcine rotavirus (S-80). Thirteen 10-day piglets were divided in four groups; Three, A group, were only given by C parvum. Four, B group, were orally administrated with firstly porcine rotavirus and then C patvum. Three, C group, were orally inoculated with porcine rotavirus alone. The rest, D group, were used as controls. During the experiment, there were daily recorded clinical signs including diarrhea to each pig. According to the periodic intervals for necropsy, all pigs were sacrificed from 4 to 12 days after the final inoculation of C parvum. Location and distribution of two pathogens, C parvum and rotavirus, in the intestinal mucosa of piglets tested were examined by pathological and immunohistological means. In addition, parasitological test using the feces of piglets was applied for the detection of cryptosporidial oocysts as well. A group showed diarrhea from 4 to 6 days post-inoculation(PI) and also discharged C parvum oocysts in feces during the day 4 to 7 PI. In tissue sections of jejunum and ileum, cryptosporidial oocysts were observed a few on the top of villi with slightly fusion. B group represented sign of diarrhea and discharge of oocysts from 2 to 11 days PI. There were some cryptosporidial oocysts both in the jejunal lumen and in the lumen of mucosal glands. As progressed, oocysts were most commonly distributed on the tip of villi of jejunum. Histopathologically there were also mild to moderately fused, attenuated focal desquamated, congested villi and mononuclear cell infiltration of varying degrees in the lamina propria of small intestine and colon at the day 4 and 7 PI. C group showed slightly to mildly attenuated and fused top of villi and mildly mucosal congestion. D group as controls was grossly and histopathologically normal in all parts of intestine. The present results indicate that the piglets inoculated with C parvum only are certainly milder in pathogenesis including duration of clinical course and severity of lesion than those in piglets concurrently infected with porcine rotavirus and C parvum. Also the strain (VRI-CN91) of C parvum used in the study has very low pathogenicity to occur enteritis of piglets.

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Epidemiological characteristics of 2002 outbreak of classical swine fever in Korea (2002년 한국에서 발생한 돼지콜레라의 역학적 특성)

  • Park, Choi-Kyu;Song, Jae-Young;Wee, Sung-Hwan;Lee, Eune-Sub;Yoon, Hachung;Moon, Oun-Kyeong;Choi, Eun-Jin;Nam, Hyang-Mi
    • Korean Journal of Veterinary Research
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    • v.46 no.2
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    • pp.107-117
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    • 2006
  • This paper described the epidemiological characteristics of 2002 outbreak of classical swine fever (CSF) in Korea. A total of thirteen CSF-infected farms could be classified into two clusters according to the location and time of outbreak. Two farms located in the same county of Gangwon province and 11 farms located in several different districts of Incheon metropolitan/Gyeonggi province were identified as CSF-infected from April 16 to 30 and from October 7 to December 21 in 2002, respectively. As the result of epidemiological analysis, the two clusters of outbreaks were turned out to be independent epidemics which had different sources of virus introduction. Three farms were found to have been infected primarily; one located in Cheolwon county of Gangwon province and two located in Kangwha county of Incheon metropolitan area. The most likely factors of virus introduction into these primary infected farms were considered to be direct or indirect contact by foreign workers and/or owners of the infected farms who had come back from traveling in China before outbreaks. This was supported by the genetic typing of CSF viruses isolated from the pigs of infected farms. All the virus isolates of 2002 outbreak were found to be genetic type 2, whereas the viruses isolated before 2000 were type 3 and the reference strains, such as attenuated live vaccine virus (LOM strain) and high virulent challenge virus (ALD strain), were type 1. Accordingly, we concluded that the 2002 CSF outbreak must have been caused by a newly introduced virus from overseas and the type 3 virus must have been eradicated after the last outbreak of 1999 by the national CSF eradication campaign which were implemented since 1996. Based on the combined analysis of epidemiological data and genetic typing, the transmission routes of classical swine fever virus were found to be the movement of vehicles (60%) and persons (10%), neighbourhood spread (20%) and unknown (10%). It is expected that the analyzed data and findings of classical swine fever outbreak epidemic could be very useful to establish the disease control and eradication program for the country in the future.

A Study on Children(3~6 years old)'s Behavior Development and Parent's Expectation (3~6세(歲) 아동(兒童)의 행동발달(行動發達)과 부모(父母)의 기대(期待)에 관(關)한 연구(硏究))

  • Park, Wee Sang
    • Korean Journal of Child Studies
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    • v.2
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    • pp.17-30
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    • 1981
  • This study is designed to analyze the child's behaviour development and consider parent's expectation toward it. In the research of the aspect of child's behaviour development, first, this study will analyze the change and development a child shows according to his age, sex, and the community where he is brought up, and then, the relationship between child's behaviour development and parent's expectation toward it. (1) Child's Behaviour Development. a) According to Age. At the age of three ; In such behaviours as wiping nose, dressing, brushing teeth, putting on coat alone, going to toilet for B.M., going on errands, and playing contentedly alone, washing hands, greeting his elders, a child at the age of three to four shows a rapid development, and therefore it seems better to train a child in these behaviors age of three. At the age of four ; In such behaviors sharing cakes with friends, riding on a tricycle, a child shows great development, at the age of four to five, and therefore it seems better to train him in them at the age of four. At the age of five ; In such behaviors as combing hair, putting toys away in proper place, telephoning a child shows great development at the age of five to six, and therefore it seems better to train him in them at the age of five. b) According to sex and community. As a whole, there is little difference between sexes and the communities. It can be estimated from this that sex or the community has on the whole no great influence on the basic aspects of child's behavior development. (2) Parent's expectation. On the whole parent's expectation falls short of a child's development in behaviors ; especially, bathing himself, dressing, going to toilet for B.M, going to toilet to urinate, telephoning, etc. This tendency is consficuous in the case of three or four year old children. This results from parent's protecting in excess their children and reducing the opportunities to encourage the children's independence by delaying the babyhood. (3) The relationship between child's behaviour development and parent's expectation. a) On the whole, the behaviour of a child is at a higher level than parent's expectation. b) As a child grows from three years to six, his behaviour develops on the whole in accordance with the increase of parent's expectation. But the level of the former is lower than that of the latter. c) According to computing a coefficient of correlation is as follows. 3 years old ; .84 4 years old ; .90 5 years old ; .87 6 years old ; .89 It shows there is a high correlation between child's Behaviour and Parent's Expectation.

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Studies on the Landslides Caused by Typhoon No. 875 in Gyeongsangnam-do Distiricts (87년(年) 태풍(颱風) 5호(號)에 의(依)한 경남지구(慶南地區)의 산사태(山沙汰)에 관(關)한 연구(硏究))

  • Kang, Wee Pyeong;Ma, Ho Seop;Jeong, Mo Geun
    • Journal of Korean Society of Forest Science
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    • v.77 no.3
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    • pp.276-282
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    • 1988
  • Many landslides occurred at the western part of Gyeongnam province by the typhoon No. 875. Their conditions and causes were investigated and were summarized as follows : The total number of landslide occurred was 2,490 and its area was 156.53ha. In about 94% of landslides and about 97% of landslides area occurred in 3 counties, Sancheong, Hamyang and Geochang. The landslides smaller than 0.01ha was 49% of the total indicating that about half of them were the small landslides. Considering homogenous topography amount of precipitation and forest type landslides appeared to be mainly has a high affected by the geological factor because the landslides mainly occurred in the deep weathered soil of igneous rock. The landslides occured on concave slopes of 31 to $35^{\circ}$ in steepness with low crown density. In the area of the deep weathered soil, landslide likely occur repeatedly. Therefore, regular erosion control works are recommended in the area of pos for disaster prevention and counter-measures.

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The Spatial Characteristics of Clinic Distribution by Specialty Subject (진료 전문과목별 개원 의원의 공간적 분포 특성)

  • Seo, Wee-Yeun;Lee, Keum-Sook
    • Journal of the Economic Geographical Society of Korea
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    • v.10 no.2
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    • pp.153-166
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    • 2007
  • This study attempts to investigate the spatial characteristics of medical hospital distributions in Korea. For the purpose we examine the inter- and intra regional variations in the distributions of clinics by specialty subject. In particular, we analyze the distribution patterns of Location Quotients of clinics for 12 specialty subjects in Seoul. Medical services tend to be concentrated on the large cities, especially in the Metropolitan Seoul Area. In particular, clinics and medical doctors as well as large scale general hospitals have been strongly concentrated on the Metropolitan Seoul area. These circumstace may be related with the fact that medical hospitals are established and operated by private sector in Korea, and thus they attempt to find their location where they can get maximum profit. The distribution patterns of clinics of 12 specialty subjects can be classified to several characteristic patterns. In particular, clinics of plastic surgeon tend to be strongly concentrated on the Gangnam area in Seoul. Finally, clinics of plastic surgeon tend to be located on the areas near the subway stations along the subway Line 2 and Line 3 in the beginning. The existence of plastic surgeons turns out to have significant role on determining the location for the newly opening plastic surgeons in the later. Therefore, their agglomeration has been getting more strongly.

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High Resolution Gyeonggi-do Agrometeorology Information Analysis System based on the Observational Data using Local Analysis and Prediction System (LAPS) (LAPS와 관측자료를 이용한 고해상도 경기도 농업기상정보 분석시스템)

  • Chun, Ji-Min;Kim, Kyu-Rang;Lee, Seon-Yong;Kang, Wee-Soo;Park, Jong-Sun;Yi, Chae-Yon;Choi, Young-Jean;Park, Eun-Woo;Hong, Sun-Sung
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.2
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    • pp.53-62
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    • 2012
  • Demand for high resolution weather data grows in the agriculture and forestry fields. Local Analysis and Prediction System (LAPS) can be used to analyze the local weather at high spatial and temporal resolution, utilizing the data from various sources including numerical weather prediction models, wind or temperature profilers, Automated Weather Station (AWS) networks, radars, and satellites. LAPS has been set to analyze weather elements such as air temperature, relative humidity, wind speed, and wind direction every hour at the spatial resolution of $100m{\times}100m$ for Gyeonggi-do on near real-time basis. The AWS data were revised by adding the agricultural field AWS data (33 stations) in addition to the KMA data. The analysis periods were from 1 to 31 August 2009 and from 15 to 21 February 2010. The comparison of the LAPS output showed the smaller errors when using the agricultural AWS observation data together with the KMA data as its input data than using only either the agricultural or KMA AWS data. The accuracy of the current system needs improvement by further optimization of analyzing options of the system. However, the system is highly applicable to various fields in agriculture and forestry because it can provide site specific data with reasonable time intervals.

Immediate breast reconstruction following nipple-sparing mastectomy in an Asian population: Aesthetic outcomes and mitigating nipple-areolar complex necrosis

  • Pek, Wan-Sze;Tan, Bien-Keem;Ng, Yvonne Ying Ru;Tan, Veronique Kiak Mien;Rasheed, Mohamed Zulfikar;Tan, Benita Kiat Tee;Ong, Kong Wee;Ong, Yee Siang
    • Archives of Plastic Surgery
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    • v.45 no.3
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    • pp.229-238
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    • 2018
  • Background Nipple-sparing mastectomies (NSMs) are increasingly performed to obtain the best aesthetic and psychological outcomes in breast cancer treatment. However, merely preserving the nipple-areolar complex (NAC) does not guarantee a good outcome. Darkly pigmented NACs and a tendency for poor scarring outcomes are particular challenges when treating Asian patients. Herein, we review the reconstructive outcomes following NSM at Singapore General Hospital. Methods All breasts reconstructed following NSM over an 11-year period from 2005 to 2015 were reviewed. Information was collected from the patients' records on mastectomy indications, operative details, and complications. Patient satisfaction, breast sensation, and aesthetic outcomes were evaluated in 15 patients. Sensation was quantified using the Semmes-Weinstein monofilament test. Results A total of 142 NSMs were performed in 133 patients for breast cancer (n=122, 85.9%) or risk reduction (n=20, 14.1%). Of the procedures, 114 (80.2%) were autologous reconstructions, while 27 (19.0%) were reconstructions with implants. Complications occurred in 28 breasts (19.7%), with the most common complication being NAC necrosis, which occurred in 17 breasts (12.0%). Four breasts (2.8%) had total NAC necrosis. The overall mean patient satisfaction score was 3.0 (good). The sensation scores were significantly diminished in the skin envelope, areola, and nipple of breasts that had undergone NSM compared to non-operated breasts (P<0.05). Half of the subset of 15 patients in whom aesthetic outcomes were evaluated had reduced nipple projection. Conclusions Immediate reconstruction after NSM was performed with a low complication rate in this series, predominantly through autologous reconstruction. Patients should be informed of potential drawbacks, including NAC necrosis, reduced nipple projection, and diminished sensation.

A Study on Dose Distribution around Fletcher-Suit Colpostat Containing Cs-137 Source by a Computer (컴퓨터 의한 Fletcher-Suit Colpostat 주변의 Cs-137의 선량분포에 관한 연구)

  • Kang Wee-Saing
    • Radiation Oncology Journal
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    • v.7 no.2
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    • pp.305-311
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    • 1989
  • Fletcher-Suit colpostat has an internal structure to reduce dose to bladder and rectum. Some programs were developed to calculate dose at any point in water in three dimension around the colpostat containing Cs-137 tube, to find the shielding effect to dose by the internal structure, and to draw isodose curves and iso-shielding effect curves. Computer was an IBM compatible AT with EGA card and language was MS-Basic V6.0, Material, shape and geometry of the strucure, tube and colpostat were considered in algorithm for calculation of dose. Dose rates per unit mg. Ra. eq. in water calculated by a program were stored in auxiliary memory devices and retrieved in another programs. Isodose curves on medial side shrinked. Dose distribution was not symmetric about a transverse axis bisecting the colpostat. Reduction of dose was more excessive on top side than on bottom. Iso-shielding effect curve showed that the shielding effect was higher on top side than on bottom, and that there was shielding effect over almost all area of medial side. Such results were related to both shifted position of tube in the colpostat and asymmetric distribution of active source in the tube. Maximum of shielding effect was $49\%$ on top side and $44\%$ on bottom side. The direction of iso-shielding effect curve was generally radial from the center of active source. In treatment planning using Fletcher-Suit colpostat, the internal structure should be considered to find precise doses to bladder and rectum, etc.

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Characteristics of Spore Density and Colonization Pattern of Arbuscular Mycorrhizal Fungi on the No-tillage Soil under Greenhouse Condition (시설재배지 무경운 토양에서 녹비작물별 Arbuscular Mycorrhizal Fungi(AMF) 감염양상과 포자밀도 특성)

  • Yang, Seung-Koo;Seo, Youn-Won;Kim, Byeong-Ho;Sohn, Bo-Kyoon;Wee, Chi-Do;Lee, Jeong-Hyun;Jung, Woo-Jin;Park, Ro-Dong
    • Korean Journal of Organic Agriculture
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    • v.19 no.3
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    • pp.343-355
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    • 2011
  • This work was studied the effects of spore density and infection of arbuscular mycorrhizal fungi (AMF) for no-tillage organic cultivation of pepper with wintering green manure crops cultivation in greenhouse field. Spore density of arbuscular mycorrhizal fungi (AMF) in green manure crops was 189 spores/30g fresh soils in control including alive spore (82 spores). Spore density of AMF in all green manure crops was totally 196~226 spores/30g fresh soil and alive spore was 84~112 spores/30g fresh soil. Spore density of AMF in soils of Pepper crop was range of 48.0~56.7 spores/30g fresh soils after cultivation of green manure crops. Infection structure of AMF was not significantly difference in soils of green manure crops and Pepper crop after cultivation of green manure crops. Infection rate of AMF in roots of green crops was low level by 2.8% in giant chickweed, 7.4% in rye, 9.3% in hairy vetch. Infection rate of AMF in roots of barley was the highest level by 20.3%. Infection rate of AMF in roots of Pepper crop was range of 5.2~7.2% after cultivation of green manure crops Also, infection rate of AMF in roots of Pepper crop was 8.1% after the harvest of barley. Infection structure of AMF in barley very well consisted of network with internal hyphae, while hairy vetch and rye tended to no network. There was not a significant relationship between spore density in soils and infection rate of AMF in rhizosphere of Pepper.

A Study on School Uniform Modification According to Lifestyle in High School Girls (여고생의 라이프스타일에 따른 교복변형행동 비교)

  • Wee, Eun-Hah
    • Journal of Korean Home Economics Education Association
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    • v.20 no.3
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    • pp.201-213
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    • 2008
  • This study was intended to examine the relationship between lifestyle and school uniform design modification in high school girls and provide basic data for guidance in the wearing of school uniform. The subjects of this study were 585 girls who were in the first and second grades of high schools throughout Gwangju. This study was executed using a questionnaire. The data from this study was processed using SPSS WIN 12.0(Kr). The results were as follows; This study hypothesized that the perception and attitude on school uniform modification would be significantly difference depending on lifestyles in high school girls. The significant differences appeared in a few items. So the results suggested that school uniform modification was a common that appeared in most high school girls. However it was affected by their lifestyle. specifically, a digital-dependence lifestyle group has a few different perceptions and attitudes on school uniform modification than the others. In other words, school uniform modification in high school girls who spend their most time in school is a general and universal clothing behavior by psychological factors rather than the effect of lifestyle. It is considered that rules concerning school uniform modification should allow students to express their individuality within certain limits. Teachers should instruct students to wear appropriate underwear rather than keep students under control with a fixed set of rules. If High school girls have a negative body image as a result of their exposed body, they should receive active educational guidance.

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