• 한국물환경학회지
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• 제36권6호
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• pp.636-650
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• 2020

A modeling system that can consider the overall water environment and be used to integrate hydrology, water quality, and aquatic ecosystem on a watershed scale is essential to support decision-making in integrated water resources management (IWRM). In adapting imported models for evaluating the unique water environment in Korea, a platform perspective is becoming increasingly important. In this study, a modeling platform is defined as an ecosystem that continuously grows and provides sustainable values through voluntary participation- and interaction-of all stakeholders- not only experts related to model development, but also model users and decision-makers. We assessed the conceptual values provided by the IWRM modeling platform in terms of openness, transparency, scalability, and sustainability. I We also reviewed the technical aspects of functional and spatial integrations in terms of socio-economic factors and user-centered multi-scale climate-forecast information. Based on those conceptual and technical aspects, we evaluated potential modeling platforms such as Source, FREEWAT, Object Modeling System (OMS), OpenMI, Community Surface-Dynamics Modeling System (CSDMS), and HydroShare. Among them, CSDMS most closely approached the values suggested in model development and offered a basic standard for easy integration of existing models using different program languages. HydroShare showed potential for sharing modeling results with the transparency expected by model user-s. Therefore, we believe that can be used as a reference in development of a modeling platform appropriate for managing the unique integrated water environment in Korea.

• 한국인터넷방송통신학회논문지
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• 제21권3호
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• pp.91-98
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• 2021

대부분 해킹 과정에서 공격자는 자신의 원점 IP(Internet Protocol) 노출을 방지하고자 다양한 우회접속 방법을 이용한다. 따라서 방어자 입장에서 공격자의 원점 IP를 식별하는 것은 공격자를 인식하는데 있어 중요한 이슈이다. 공격자가 프록시(proxy)를 이용할 경우에는 웹 브라우저가 아닌 다른 응용프로그램을 통해서 원점 IP 확인이 가능하다. 그러나 이러한 방법은 VPN(Virtual Private Network)을 이용할 경우에는 아무런 효과가 없다. VPN은 모든 애플리케이션에 영향을 미치기 때문이다. 학술적으로는 네트워크 장비를 이용하여 IP를 역추적하는 다양한 방법이 연구되고 있으나 표준화, 개인정보보호 등의 문제로 인해 실현되기에는 아직 요원한 상태이다. 이러한 한계를 극복하기 위해 본 논문에서는 VPN을 사용하는 접속자의 네트워크 라우팅 테이블 정보를 이용하여 원점 IP를 탐지하는 실제적인 방법을 제안한다. 이 방법은 접속자의 네트워크 사용에는 영향을 미치지 않으며 개인정보 또한 수집하지 않는다. 더구나 다양한 VPN 툴을 사용하여 실제 인터넷에서 제안된 방법을 구현하고 검증하였다.

• Entomological Research
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• 제48권5호
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• pp.390-399
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• 2018

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real-time reverse transcription polymerase chain reaction (RT-qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including ${\beta}$-actin (${\beta}$-ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde-3-phosphate (GAPDH), elongation fator-alpha ($EF1{\alpha}$), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha-tubulin (${\alpha}$-TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software-based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web-based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both $RPL7/EF1{\alpha}$ were suitable for experiments of different tissues, whereas for insecticide treatments, $28S/{\alpha}-TUB$ were suitable for normalizations of expression data. In addition, $28S/{\alpha}-TUB$ were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

• 정보보호학회논문지
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• 제22권2호
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• pp.379-390
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• 2012

본 연구는 2008년부터 2011년까지 행정안전부에서 시행하고 있는 공공기관의 개인정보보호 수준진단 지표의 정책적 의미를 고찰하고, 각 지표의 중요도 선정에 따른 개인정보보호의 정책적 문제를 다루었다. 우선, 개인정보보호수준진단 지표를 선정하기 위해 개인정보보호에 대한 정책 방향을 개인정보보호의 정책 및 기술적 측면, 개인정보의 관리적 측면, 개인정보 침해 대응적 측면인 세 분야로 구분하였다. 그리고, 각 정책방향에 따라 선정된 지표의 세부사항에 대해 학자, 전문가, 실무자를 대상으로 AHP 조사를 실시하여 우선적으로 추진해야 할 정책을 정하도록 하였다. AHP를 통해 개인정보보호 수준 진단 지표의 중요도를 도출하였으며, 개인정보보호정책의 일반적인 체계를 갖추기위해 필요한 정책과제를 도출하였다. 결과적으로 공공기관의 개인정보보호의 제도적 기반 확충을 위해서는 웹사이트상의 개인정보관리, 개인정보보호를 위한 전담 조직 및 인력, 그리고 개인정보보호시스템 도입 등이 우선 시 된다. 본 연구의 정책과제를 기초로 공공기관의 개인정보보호 수준진단 체계가 지속된다면 개인정보를 취급하는 공공기관에 대한 국민의 신뢰가 높아지고, 개인정보의 안전한 유통 및 가치가 향상되리라 기대한다.

• Journal of Microbiology and Biotechnology
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• 제31권6호
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• pp.882-889
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• 2021

In order to use an enzyme industrially, it is necessary to increase the activity of the enzyme and optimize the reaction characteristics through molecular evolution techniques. We used the error-prone PCR method to improve the reaction characteristics of LipCA lipase discovered in Antarctic Croceibacter atlanticus. Recombinant Escherichia coli colonies showing large halo zones were selected in tributyrin-containing medium. The lipase activity of one mutant strain (M3-1) was significantly increased, compared to the wild-type (WT) strain. M3-1 strain produced about three times more lipase enzyme than did WT strain. After confirming the nucleotide sequence of the M3-1 gene to be different from that of the WT gene by four bases (73, 381, 756, and 822), the secondary structures of WT and M3-1 mRNA were predicted and compared by RNAfold web program. Compared to the mean free energy (MFE) of WT mRNA, that of M3-1 mRNA was lowered by 4.4 kcal/mol, and the MFE value was significantly lowered by mutations of bases 73 and 756. Site-directed mutagenesis was performed to find out which of the four base mutations actually affected the enzyme expression level. Among them, one mutant enzyme production decreased as WT enzyme production when the base 73 was changed (T→ C). These results show that one base change at position 73 can significantly affect protein expression level, and demonstrate that changing the mRNA sequence can increase the stability of mRNA, and can increase the production of foreign protein in E. coli.

• 대한통합의학회지
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• 제9권1호
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• pp.59-67
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• 2021

Purpose : This study examined the factors related to sexual intercourse among adolescents in South Korea. Methods : The study sample was comprised of 65,528 middle and high school students aged 12 to 18 years in 2016. For this study, using the data from the 12th Korea youth risk behavior web-based survey, a chi-squared test, and hierarchical logistic regression analysis we reconducted with the SPSS 22.0 program considering personal, family, school, and regional characteristics. Results : The proportion of adolescents who had sexual intercourse was 6.0 % of boys and 2.7 % of girls, and 6.3 % of high school students and 2.4 % of middle school students. The statistically significant related factors of sexual intercourse were gender, grade, attempted suicide, ever drinking, ever smoking, habitual or purposeful drug use, weekly allowance, and coed school in the final model 4. Adolescents with suicide attempts had 2.49 times more sexual intercourse than adolescents with no experience of suicide attempts. Compared to adolescents without alcohol, smoking, and habitual drug use, those who have experienced alcohol, smoking, habitual or purposeful drug use were 2.22 times, 3.76 times, and 3.39 times more likely to have had sexual intercourse, respectively. Adolescents with a weekly allowance of more than 100,000 won per week and adolescents in coed schools were 2.84 times and 1.40 times more likely to have had sexual intercourse, respectively. Conclusion : Multi-level interventions should be considered in school sexual education programs while considering the substantiality of sex education and the actual adolescents' needs. In addition, sexual health interventions in cyberspace for high-risk groups may be needed to consider the emotional and mental characteristics, given the factors related to sexual intercourse in adolescents.

• 문화기술의 융합
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• 제8권4호
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• pp.375-380
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• 2022

본 연구는 빅데이터를 활용하여 편의점 간편식에 대한 소비자들의 인식과 의미를 알아보기 위한 목적이 있다. 이 연구를 위하여 네이버(NAVER)와 다음(Daum)에서 뉴스, 지식인, 블로그, 카페, 지식인(팁), 웹 문서를 대상으로 분석하였고 자료 검색을 위한 키워드로는 '편의점 간편식'을 사용하였다. 자료 분석 기간은 2019년 1월1일부터 2021년 12월 31일까지 3년으로 선정하였다. 자료수집 및 분석을 위해서는 텍스톰(TEXTOM)을 사용하여 빈도 및 매트릭스 데이터를 추출하였고 UCINET 6 프로그램의 NetDraw 기능을 이용해 네트워크 분석과 시각화 분석을 실시하였다. 그 결과 편의점 간편식을 소비자들의 선택속성에 따라 건강성, 다양성, 간편성, 경제성으로 군집화 하였다. 직접 조리한 음식에 뒤떨어지지 않고 한 끼 식사로 그 종류가 다양하며, 적절한 가격, 할인 쿠폰, 이벤트 등 편의점 간편식에 대한 소비자들의 의미와 선택속성을 바탕으로 간편성과 편의성을 추구하는 변화된 생활방식에 맞는 새로운 간편식 메뉴 개발에 기초 자료가 되기를 기대한다.

• 산업융합연구
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• 제20권1호
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• pp.87-96
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• 2022

본 연구는 알레르기 질환을 가진 청소년들의 실태와 정신건강과 관련된 요인을 파악 하고자 한다. 질환 관리를 위한 기초자료를 제공 하고자 함이다. 연구방법은 제16차 청소년 건강행태 조사 빅데이터를 이차분석 하였으며, SPSS 25 프로그램을 이용하여 x²-test를 사용하고 연관 정도를 파악하기 위해 오즈비와 95% 신뢰구간을 구하였으며, 다중 로지스틱 회귀분석을 시행하였다. 연구 결과 알레르기 질환을 가지고 있는 청소년의 37.1%가 스트레스 인지가 있는 것으로 나타났으며, 우울감 경험률이 27.6%, 자살 생각률이 12.5% 처럼 알레르기 질환을 가지고 있는 청소년의 스트레스 인지, 우울감 경험 및 자살생각의 가능성이 알레르기성 질환을 가지고 있지 않은 청소년에 비해 높았다. 본 연구 결과로 청소년의 알레르기 질환 유무에 따라 정신건강에 많은 영향을 미치는 것을 알 수 있었고 정신질환을 초래할 수 있는 질환 및 청소년 정신건강 관련 예방과 연구에 기초 자료로 삼고자 한다.

• 한국미생물·생명공학회지
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• 제49권1호
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• pp.65-74
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• 2021

Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.

• 대한통합의학회지
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• 제10권2호
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• pp.61-72
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• 2022

Purpose : This study aimed to identify the educational effects of the International Development Cooperation and Healthcare course from 2018 to 2020. Methods : Changes in awareness of international development cooperation (understanding international development cooperation as well as government Official Development Assistance [ODA], thoughts on aid, and thoughts on government's aid scale) were analyzed using web-based surveys. The pre-course survey was conducted 1-2 weeks before the semester started, and the post-course survey was conducted 1-2 weeks after the semester ended. All data were analyzed using the SPSS 26.0 program, and statistical significance was set at p< .05. Results : A total of 314 people participated in the survey before taking the course, and 286 people participated in the survey after taking the course. Analysis of the changes in students' awareness of international development cooperation showed that all items (understanding international development cooperation and government ODA, as well as having thoughts on aid and government's aid scale) were improved. Regarding changes in awareness according to general characteristics, thoughts on aid were statistically significant according to all characteristics except for 2018. Regarding government's aid scale, awareness scores increased after taking the course in students who majored in healthcare, fine arts and athletics, broadcasting and media studies, and digital contents. Conclusion : This study showed that changes in awareness of international development cooperation were largest in second-year students. Compared to changes regarding international development cooperation, government ODA, and thoughts on aid, changes regarding thoughts on government's aid scale were minor. In the future, it is necessary to develop class content suitable for the first-year level and to supplement and operate content that can change the way of thinking on government's aid scale.