• Nutrition Research and Practice
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• 제11권2호
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• pp.121-129
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• 2017

BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets. MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a $2{\times}2$ factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication. RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), ${\alpha}$-ketoglutarate dehydrogenase (${\alpha}$-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-${\gamma}$ coactivator $1{\alpha}$ (PGC-$1{\alpha}$). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, ${\alpha}$-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-$1{\alpha}$, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, $H^+$ transporting, mitochondrial F1 complex, ${\beta}$ polypeptide in liver. CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1325-1335
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• 2018

Objective: This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods: Eighty female piglets were randomly distributed to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight/24 h) or to be provided with restricted nourishment supplemented with normal saline for 7 h or 3, 6, or 9 d in pairs. Results: Regardless of administration time, spermine supplementation significantly up-regulated cyclin A2 gene expression but down-regulated p21 and cyclin D3 mRNA levels in the thymus and spleen and reduced cyclin E2 gene expression in the thymus of piglets (p<0.05). Irrespective of the treatment period, the reduced Bax and caspase-3 gene expressions and improved Bcl-2 mRNA level were observed in the thymus and spleen of spermine-administrated piglets (p<0.05). Regardless of supplementation time, spermine intake significantly enhanced the expressions of amino acid transporter-related genes (SLC1A1, SLC1A5, SLC7A1, SLC7A7, and SLC15A1) in both thymus and spleen, as well as SLC7A9 in the spleen of piglets (p<0.05). In addition, extended spermine administration also markedly promoted cell proliferation, depressed apoptosis and modulated amino acid transport (p<0.05), and such effects were the greatest during prolonged spermine supplementation (6 d) compared to the other time periods (p<0.05). Conclusion: Spermine supplementation may regulate cell cycle during the G1/S phase, suppress apoptosis and modulate amino acid transport. A period of 6 d of spermine supplementation is required to produce the optimal effects on nutritional implications.

• 한국동물위생학회지
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• 제34권4호
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• pp.341-352
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• 2011

Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 ($1{\times}10^8$ CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C, $1.0{\times}10^6$ PFU/g; Group D, $1.0{\times}10^8$ PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P<0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P<0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P<0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.

• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1734-1744
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• 2019

Objective: In this study, two glycosidases (XMosidases), ${\beta}$-xylosidase and ${\beta}$-mannosidase, were investigated on their in vitro hydrolysis activities of feed and on the improvement of growth performance in vivo in weanling pigs. Methods: Enzyme activities of XMosidases in vitro were evaluated in test tubes and simulation of gastric and small intestinal digestion, respectively, in the presence of NSPase. In vivo study was performed in 108 weaned piglets in a 28-d treatment. Pigs were allotted to one of three dietary treatments with six replicate pens in each treatment. The three treatment groups were as follows: i) Control (basal diet); ii) CE (basal diets+CE); iii) CE-Xmosidases (basal diets+ CE+${\beta}$-xylosidase at 800 U/kg and ${\beta}$-mannosidase at 40 U/kg). CE was complex enzymes (amylase, protease, xylanase, and mannanase). Results: In vitro XMosidases displayed significant activities on hydrolysis of corn and soybean meal in the presence of non-starch polysaccharide degrading enzymes (xylanase and ${\beta}$-mannanase). In vitro simulation of gastric and small intestinal digestion by XMosidases showed XMosidases achieved $67.89%{\pm}0.22%$ of dry matter digestibility and $63.12%{\pm}0.21%$ of energy digestibility at $40^{\circ}C$ for 5 hrs. In weanling pigs, additional XMosidases to CE in feed improved average daily gain, feed conversion rate (p<0.05), and apparent total tract digestibility of crude protein (p = 0.01) and dry matter (p = 0.02). XMosidases also altered the gut bacterial diversity and composition by increasing the proportion of beneficial bacteria. Conclusion: Addition of a complex enzyme supplementation (contained xylanase, ${\beta}$-mannanase, protease and amylase), XMosidases (${\beta}$-xylosidase and ${\beta}$-mannosidase) can further improve the growth performance and nutrient digestion of young pigs.

• 농업과학연구
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• 제48권3호
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• pp.397-412
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• 2021

In Experiment 1, the impact of using diets sufficient in P on the performance of weaned piglet growth with or without a super dose of phytase was investigated. One hundred and twelve piglets were randomized into four treatments with 7 replicates (4 piglets·cage^-1). Bodyweight (BW), daily gain (ADG), daily intake (ADFI) and feed conversion ratios (FCR) were measured over three weeks. Treatments included a positive control (PC) with a Ca : P ratio of 0.80 : 0.50%. and the negative control (NC) had a Ca : P ratio of 0.60 : 0.50%. The PC plus phytase was denoted as PC+, and the NC with the phytase diet was designated as NC+. The PC diet was formulated to meet or exceed the NRC (2012) standards for pigs weighing 7 - 25 kgs. Better results were obtained with the PC diet (p > 0.1) compared to the NC diet. The super dose of phytase in the PC+ diet significantly improved the overall FCR (p < 0.1). For Experiment 2, the impact of different calcium (Ca)/phosphorous (P) ratios with or without supplementation of vitamin D₃ was determined. Ninety-six piglets were randomized into four treatments with 6 replicates (4 piglets·cage^-1). The PC, NC1 and NC2 diets had ratios of 0.80 : 0.50, 0.80 : 0.45, and 0.60 : 0.45%, respectively. The fourth diet (PCV) was the PC diet fortified with vitamin D₃. Improved results were observed with the PC diet (p > 0.1) compared to the NC diets. Supplementing dietary 25-OH-D₃ in the PCV diet led to numerically higher ADG and ADFI scores with lower FCR values at the early nursery stage (p = 0.0044), but the vitamin supplement did not significantly influence the growth.

• Animal Bioscience
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• 제35권2호
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• pp.260-271
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• 2022

Objective: We aimed to investigate the effect of the differing amino acid (AA) release dynamics of two protein sources on the growth performance, nitrogen deposition, plasma biochemical parameters, and muscle synthesis and degradation of piglets when included in their diets at normal and low concentrations. Methods: Forty-eight piglets (Duroc×Landrace×Large White) with initial body weight of 7.45±0.58 kg were assigned to six groups and fed one of 6 diets. The 6 dietary treatments were arranged by 3×2 factorial with 3 protein sources and 2 dietary protein levels. They are NCAS (a normal protein content with casein), NBlend (a normal protein content with blend of casein and corn gluten meal), NCGM (a normal protein content with corn gluten meal), LCAS (a low protein content with casein), LBlend (a low protein content with blend of casein and corn gluten meal), LCGM (a low protein content with corn gluten meal). The release dynamics of AA in these diets were determined by in vitro digestion. The digestibility, utilization and biological value of nitrogen in piglets were determined by micro Kjeldahl method. Plasma insulin was measured by enzyme-linked immunosorbent assay kits. The protein expression of mediators of muscle synthesis and degradation was determined by western blotting. Results: Although the consumption of a low-protein diet supplemented with crystalline AA was associated with greater nitrogen digestion and utilization (p<0.05), the final body weight, growth performance, nitrogen deposition, and phosphorylation of ribosomal protein S6 kinase 1 and eIF4E binding protein 1 in the muscle of pigs in the low-protein diet-fed groups were lower than those of the normal-protein diet-fed groups (p<0.05) because of the absence of non-essential AA. Because of the more balanced release of AA, the casein (CAS) and Blend-fed groups showed superior growth performance, final body weight and nitrogen deposition, and lower expression of muscle ring finger 1 and muscle atrophy F-box than the CGM-fed groups (p<0.05). Conclusion: We conclude that the balanced release of AA from CAS containing diets and mixed diets could reduce muscle degradation, favor nitrogen retention, % intake and improve growth performance in pigs consuming either a normal- or low-protein diet.

• Journal of Animal Science and Technology
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• 제54권1호
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• pp.9-14
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• 2012

본 연구는 국내 특정 종돈장에서 보유하고 있는 종돈에 대한 번식형질 및 산육형질들에 대한 유전변이를 추정하고자 연구를 실시하였다. 본 분석에 이용된 자료는 2000년부터 2008년까지 개량을 진행한 국내 모종돈장에 있는 돼지(Landrace, Large white, Duroc) 2,447두에서 조사된 9,886복의 번식자료(실산자수, 이유자 돈수)와 10,181두의 산육검정자료(등심단면적, 90 kg 도달일령, 등 지방두께, 정육률)을 이용하여 분석을 실시하였다. 번식형질 및 산육형질에 적합한 모형을 찾기 위해 분산분석을 실시하였으며, 그 결과 번식형질에서 품종효과, 교배웅돈효과, 산차효과, 분만시 년도-계절효과, 산육형질에서 품종효과, 분만시 년도-계절효과, 성의 효과, 모산차 등이 환경요인으로 작용하는 것으로 나타나 이들을 혼합모형방정식에 적합시켜 유전모수를 추정하였다. 그 결과, 번식 형질의 실산자수에 대한 유전력은 0.07, 모체 유전효과에 대한 유전력은 0.02로 추정되었고 이유자돈수의 유전력은 0.03, 모체 유전효과에서 0.02로 추정되었으며 이들 두 형질 간의 유전상관은 0.14, 모체 유전효과에 대한 상관은 0.06로 추정되었다. 또한 등심 면적의 경우 0.19, 90 kg 도달일령은 0.39, 등지방두께 및 정육률은 각각 0.36 및 0.43으로 번식형질에 비해 높은 유전력을 나타내는 것으로 추정되었다. 또한 등심단면적과 등지방두께 간에는 0.04로 유전적 상관관계가 없으며, 등심단면적과 정육률 간에는 0.35로 중도의 유전적 상관관계를 갖는 것으로 추정되었다. 반면에 등지방 두께와 정육률 간은 -0.42로 중도의 부의 상관관계를 나타냈으며, 등지방두께와 90 kg 도달일령 간에는 유전상관이 없는 것(0.00)으로 추정되었다. 또한 번식형질과 산육형질 간 유전상관은 등지방두께를 제외한 나머지 산육형질에서 번식형질과의 부의 상관을 보여, 번식형질 또는 산육형질을 독자적으로 개량하고자 할 때, 부의 관계가 있는 형질과의 유전적 관계를 고려한 개량목표 설정이 필요할 것으로 사료된다.

• 한국축산시설환경학회지
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• 제7권1호
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• pp.1-12
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• 2001

조기이유 자돈용 액상사료 지동 급이기를 개발하기 위하여 물 가열 및 배출부, 가루사료 저장 및 배출부, 혼합부, 약상사료 이송부와 이들의 각 기능을 제어하는 중앙 제어부 및 급이기로 구성 하였다. 이러헥 개발한 조기이유 자돈용 액상사료 자동급이기의 실용성을 검토하기 위해 평균 19일령에 이유한 삼원교잡종(Lw$\times$D)ㅈ돈 18두(남9,여9)를 공시하여 3주 동안 액상으로 급여한 후 3주 동안 가루사료를 무제한 급여하였다. 결과는 아래와 같다. 개발한 액상사료 자동 급이기의 물 가열 및 배출부는 혼합부에 온수를 초당 27$m\ell$, 가루사료 저장 및 배출부는 가루사료를 초당 3.7g 씩 정확하게 중앙제어부의 작동에 따라 이송하였다. 일당증체량은 NRC의 표준 성장률과 비교해 액상사료를 급여한 3주 동안 자돈들이 10%정도 낮은 수준이었으나, 그후 가루사료를 급여한 3주 동안에는 24%, 시험전기간 17% 정도 높은 수준이었다. 사료요구율은 시험개시 후 3주 동안 1.09, 그 후 3주동안 2.14, 시험전기간에는 1.89 이었다. 설사 발생은 액상사료 급여후 3일부터 7일 까지 5일동안 발생하였고, 폐사 자돈은 없었다. 새로이 개발한 액상사료 자동 급이기 시작품의 성능 검정을 위한 예비적 시험으로 19일령에 이유한 자돈에게 적용해 본결과, 액상사료 자동급이기는 어떠한 문제없이 잘 작동되었으며, 자돈들의 액상사료 급여 초기 성장은 부진하였으나 차후 성장 촉진과 사료 이용율이 개선되는 것으로 나타났다. 따라서 본 연구에서 새로이 개발한 액상사료 자동 급이기를 이용한 자돈 사육이 가능할 것으로 사료 된다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1485-1491
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• 2005

This experiment was conducted to investigate the effects of iron from an amino acid complex (Availa-$Fe^{\circledR}$) on the iron status of neonatal and suckling piglets. A total of 24 gestating sows (Landrace${\times}$Large White) were randomly allocated to three dietary treatments. The control diet contained 80 mg $kg^{-1}$ Fe from ferrous sulfate heptahydrate ($FeSO_4$.$7H_2O$), while the two experimental diets were supplemented with an additional 120 mg $kg^{-1}$ Fe from Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$, respectively. The lactating sows remained the same iron treatments as gestating sows, while neonatal piglets of 24 litters born from the above sows were allotted to another three treatments. Piglets from the sows of the control treatment were fed basal diet with no supplemental Fe as control treatment, but were injected with 100 mg Fe as Fe dextran at birth. Piglets from the sows of Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$ treatments were supplemented with 120 mg $kg^{-1}$ iron from Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$, respectively. The total born alive and weaned, and the average piglets weight at birth and at weaning were not significantly affected by the sow' dietary treatments (p>0.05). Iron from Availa-$Fe^{(R)}$ did not demonstrate a statistically significant improvement in hemoglobin concentration, hematocrit and plasma iron of sows on day 90 and 105 of pregnancy and the milk iron of sows during lactation (p>0.05). Neonatal piglets in the Availa-$Fe^{(R)}$ treatment had a significantly higher hemoglobin concentration (p<0.05) and higher hematocrit and plasma iron (p>0.05) than those in the other two treatments, respectively. The hemoglobin of suckling piglets in the Availa-$Fe^{(R)}$ treatment was higher than that of piglets in $FeSO_4$.$7H_2O$ treatment on day 28 (p<0.05). The total iron binding capacity of piglets in Availa-$Fe^{(R)}$ treatment was lower than that of piglets in the control and $FeSO_4$.$7H_2O$ treatment on day 14 (p<0.05), but there was not a statistically significant difference among three treatments on day 28 (p>0.05). However, the hemoglobin and hematocrit of suckling piglets injected with Fe were higher than those of piglets in the other two treatments (p<0.05). This study indicated that the addition of 120 mg $kg^{-1}$ iron from amino acid complex into the diets improved iron status of neonatal and nursing piglets more effectively than the addition of 120 mg $kg^{-1}$ iron from $FeSO_4$.$7H_2O$, however, this improvement of the organic Fe was not sufficient to replace the Fe injection for prevention of iron-deficiency anemia.