• Title/Summary/Keyword: Water purification

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방선균이 생성하는 단백질 가수분해효소 저해물질의 정제 및 특성 (Purification and Properties of Protease Inhibitor from Streptomyces sp. SK-862)

  • 김중배
    • 한국식품영양학회지
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    • 제11권6호
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    • pp.678-682
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    • 1998
  • A strain of Streptomyces sp. SK-862, isolated from soil in Wonju city, was able to prodce a biologically active substance that has a strong inhibitory activity against proteolsis by trypsin. The inhyibitory substance was extracted by n-butanol, and then purified by the adsorption chromatography followed by the reverse-phase high performacne liquid chromatography. The purified substance was stable over the pH range from 2 to 10, but was unstable when treated at 8$0^{\circ}C$ for 60 min. This substance was soluble in water, methanol, ethanol nd butanol, but insoluble in chlorofrom and ethylacetate. The Rf value of the purified substance on the thin layer chromatography were 0.56 in n-butanol : methanol : water(5 : 3 : 1v/v) solvent system compare dto 0.23 in ethanol : ammonium hydoxide : water(8 : 1 : 1v/v) solvent system. This substance has maximum absorption at 259 nm. The chemical reaction of the substance was negative for sugar but positive for ninhydrine and iodine reaction.

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A Study on the Purification of Water-Pool in Irradiated Materials Examination Facility

  • Song, Ung-Sup;Lee, Jong-Heon;Lee, Hong-Gyee;Hong, Kyon-Pyo
    • 한국방사성폐기물학회:학술대회논문집
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    • 한국방사성폐기물학회 2004년도 Proceedings of the 4th Korea-China Joint Workshop on Nuclear Waste Management
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    • pp.42-50
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    • 2004
  • The pool $(3m{\times}6m{\times}10m{\times}$ in Irradiated Materials Examination Facility is generally used to transport irradiated materials between a moving cask and hot-cell. During the operation in the pool such as loading/unloading the cask, holding specimen and bucket elevation, water maybe contaminated by radioactive or contaminated impurities from irradiated materials. Then, it must be purified and filtered continuously to keep lower radioactivity than that of regulation prescribed by RCA Korea Activity in a part of radioactive contamination control. This paper described radioactive contamination distribution of water as transported materials, which is related to effective operation of purification and filtration system.

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Purification, Characterization and Application of a Cold Active Lipase from Marine Bacillus cereus HSS

  • Hassan, Sahar WM.;Abd El Latif, Hala H.;Beltagy, Ehab A.
    • 한국미생물·생명공학회지
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    • 제50권1호
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    • pp.71-80
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    • 2022
  • Lipases (triacylglycerol acylhydrolases [EC 3.1.1.3]) are water-soluble enzymes. They catalyze the hydrolysis of fats and oils. A cold-active lipase from marine Bacillus cereus HSS, isolated from the Mediterranean Sea, Alexandria, Egypt, was purified and characterized. The total purification depending on lipase activity was 438.9 fold purification recording 632 U/mg protein. The molecular weight of the purified lipase was estimated to be 65 kDa using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The optimum substrate concentration, enzyme concentration, pH, and temperature were 1.5 mM, 100 µl, pH 6 and 10℃, respectively. The lipase was tolerant to NaCl concentrations ranging from 1.5 to 4.5%. The lipase was affected by the tested metal ions, and its activity was inhibited by 16% in the presence of 0.05 M SDS. The application of the cold-active lipase for the removal of an oil stain from a white cotton cloth showed that it is a promising biological agent for the treatment of oily wastes and other related applications. To the best of our knowledge, this is the first report of the purification and characterization of a lipase from marine B. cereus HSS isolated from the Mediterranean Sea.

기후변화 대응을 위한 미국 지하수 기술 특허네트워크 분석과 주요 특허 기술 동향 (U.S.'s Patent Network Analysis and Technology Trends on Underground Water for the Response of Climate Change)

  • 윤순욱;최한나;김민철
    • 에너지공학
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    • 제28권3호
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    • pp.55-64
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    • 2019
  • 본 연구에서는 기후변화 대응의 관점에서 미국 지하수 기술을 특허 네트워크분석을 통해 핵심특허를 파악 할 수 있었다. 분석된 핵심 특허를 살펴보면, 오염된 지하수를 중금속 제거기술을 사용하여 지하수가 오염되지 않게 하여 음용수와 오염 지하수 문제를 해결할 수 있는 방법으로 높은 영향력을 보였고 기술 간 전파 되고 있었다. 또한 지하수 기술 특허 간의 특허들이 매개역할을 담당하고 있었지만 지하수 기술 간의 특허의 연결성은 높지 않게 나타나고 있다. 지하수 관련 특허 기술은 크게 양수, 모니터링, 오염정화로 구분이 가능하였다. 모니터링은 지하수 유량, 흐름 또는 수질 분석을 위한 지표, 지리적인 특성뿐만 아니라 오염물질의 종류, 농도 등 물리화학 및 생물학적 특성 파악을 가능하게 하는 기술을 포함한다. 오염정화 기술은 토양 및 지하수 정화를 위한 원위치 또는 비원위치에 적용된 물리화학적 및 생물학적 정화 처리 공정을 말한다. 미국의 기술적 사례를 발굴하여 분석하면서 미국이 수처리 분야에서 기술력이 높았고 기후변화로 인한 물부족 문제에 대응하기 위해서는 이러한 기술진흥과 특허를 통한 지재권보호도 필요한 것으로 보인다.

시설원예단지의 생태계서비스 기능 증진을 위한 개선방안 연구 (A study on the improvement of Ecosystem Service Function for the Protected Horticulture Complex in Agricultural Landscape)

  • 손진관;공민재;강동현;이시영
    • 농촌계획
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    • 제21권4호
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    • pp.45-53
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    • 2015
  • Agriculture, rural landscapes are accompanied by a variety of environmental issues. Therefore, it is necessary to study on biodiversity and ecosystem services. Horticulture complex is low groundwater recharge function, it can be evaluated as a facility that biodiversity is impaired. The ecosystem services in agricultural landscapes were obtained 19 kinds of functions. Experts survey Groundwater recharge function (4.13) teeth chapter higher, Water storage (4.05), Amphibian & Reptile habitat (3.96), Aquatic insect habitat (3.92), Flood control (3.87), Water purification (3.86), Avian habitat (3.76 ), Creating landscape (3.74), Vegetation diversity (3.71), Experience, Education (3.69), Biological control (3.48), Fishery habitat (3.42), Climate regulation (3.30), Mammal habitat (3.30), Air quality regulation (3.25 ), Mainenance of genetic diversity (3.25), were analyzed in order Rest area (3.14). Improving capabilities in the Detention Pond, Wetland, Green space, Corridor, Non-Chemical, Program development, Green spaces, Rainwater storage facilities, Water cycle system, Surface water storage facilities, Infiltration trench, Water purification facilities, Permeable pavement. Environmentally friendly, and to contribute to sustainable agricultural development through ecological planning.

옹기 필터를 이용한 무 전원 정수 장치에 관한 연구 (Characteristics of the Non-electric Water Purification System Using Onggi Filter)

  • 위인희;신동욱;한규성;김진호;조우석;황광택
    • 한국세라믹학회지
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    • 제51권4호
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    • pp.332-336
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    • 2014
  • A non-electric water purification system using the Korean traditional ceramic ware Onggi, was demonstrated as an appropriate technology to solve water shortages in under developed regions. Generally, Onggi is produced using large size raw materials that are sintered at low temperature, resulting in a porous body that shows air and water permeability. An Onggi filter was prepared using a spinning wheel with the addition of rice bran to the body to increase porosity. The porosity of the obtained Onggi filter was 25.1%; the water permeability was 85.1 $L/m^2h$. Turbidity and TDS of the purified water using Onggi filter were decreased by 97.7% and 29.1%, respectively.

수산화라디칼과 오존에 의한 수중 천연 지방산 분해 제거 연구 (Purification of the Waste Water Containing Natural Fatty Oil by Hydroxy Radical and Ozone)

  • ;원정하;김용주;고장면;송근한;이창훈
    • Korean Chemical Engineering Research
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    • 제51권4호
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    • pp.523-526
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    • 2013
  • 본 연구에서는 수질정화 기술개발을 위하여 수산화라디칼 및 오존 발생기를 이용하여 수중에 존재하는 천연 지방산 분해 제거 연구를 수행하였다. 천연 지방산은 수산화라디칼 및 오존에 의하여 1차 분해반응 형태로 제거되었으며, 천연 지방산의 분해반응에서 수산화라디칼 단독으로 사용하는 것 보다 오존과 함께 사용한 경우 분해 효율을 크게 향상시킬 수 있음을 알 수 있었다. 또한, 천연 지방산이 수산화라디칼과 오존에 의해 분해되는 화학반응 기구를 제안하였다.

소독부산물 최소화를 위한 운영조건 연구 (Operating Conditions for Minimization of DBPs (Disinfection by-Products) in Drinking Water Supply System)

  • 신형순;최필권;김종수;최일우;김상훈;김태현;이경희;이수문;장은아;정연훈;김주열
    • 상하수도학회지
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    • 제19권3호
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    • pp.330-337
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    • 2005
  • This study was carried out to propose the managemental improvement of the purification plants and the distribution facilities which can minimize the formation of disinfection by-products in drinking water distribution system. The disinfection by-products were highly created in the water treatment plant that the organic matters were high and the chlorine dosage was excessive. The concentration of DSPs was shown the highest value in August and the lowest value in December, because of temperature and pre-chlorine dosage effect. From the result of tracer test, the travel time from the treatment plant to the end of pipeline was around 3-4 days in summer, 5-6 days in winter, respectively, and the DSPs concentration of the reservoir(end of pipe) was 2-3 times higher than that of the beginning point. The improvement of the chlorination process and structural reformation of distribution facility was demanded to minimize the DSPs increase from purification plant to the end of pipe.