The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 11 Issue 6
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- Pages.678-682
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- 1998
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Purification and Properties of Protease Inhibitor from Streptomyces sp. SK-862
방선균이 생성하는 단백질 가수분해효소 저해물질의 정제 및 특성
Abstract
A strain of Streptomyces sp. SK-862, isolated from soil in Wonju city, was able to prodce a biologically active substance that has a strong inhibitory activity against proteolsis by trypsin. The inhyibitory substance was extracted by n-butanol, and then purified by the adsorption chromatography followed by the reverse-phase high performacne liquid chromatography. The purified substance was stable over the pH range from 2 to 10, but was unstable when treated at 8