• Title/Summary/Keyword: Water microbiology

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Isolation and Characterization of Biofouling Bacteria in Ultra-high Purity Water Used in the Semiconductor Manufacturing Process

  • Kim, In-Seop;Lee, Kye-Joon
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.554-558
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    • 2000
  • Bacteria were isolated and identified from an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16-megabyte DRAM semiconductor manufacturing. Scanning electron microscopic and microbiological observations revealed that the primary source of the bacteria isolated from the UHPW was detached cells from biofilms developed on the pipe wall through which the UHPW, a man-made and extremely nutrient poor environment, was passing. About 63-65% of the bacteria isolated from the UHPW and the pipe wall were Gram-positive, whereas only 10% of the bacteria isolated from the feed water were Gram-positive. The of Gram-positive bacteria and seven genera of Gram-negative bacteria. Strains of the UHPW bacteria effectively adhered to and formed a biofilm on the surface of polyvinyl chloride (PVC) pipe.

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Enhancement of the Escherichia coli Floc Strength with Water Soluble Polymers

  • KIM, CHAN-WHA;CHOKYUN RHA
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.282-286
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    • 1997
  • The floc strength of Escherichia coli was enhanced by adding water soluble polymer flocculants (BPA-5020 and BPA-5000) to the particulate flocculant (BPA-1000) as indicated by the increase in the shear index. The shear index of the E. coli flocs increased from 0.39 with the particulate flocculant alone to 0.94 with the particulate flocculant in conjunction with the water soluble polymer flocculant. In addition, the sedimentation rate of flocs was higher and the sedimented volume of flocs was smaller when the particulate flocculant was used with the water soluble polymer flocculant. When E. coli was flocculated first with the water soluble flocculant and the particulate flocculant was added later into the E. coli flocs formed, the sedimentation rate of the flocs was greater than that of any other combination. The shear index of the flocs was, however, independent of the sequence of the flocculant addition.

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Production of Photosynthetic Bacterial Cells of Rhodospirillum rubrum P17 from Soybean Curd Waste Water (두부공업폐수를 이용한 광합성세균 Rhodospirillum rubrum P17의 균체생산)

  • 강성옥;조경덕;임완진;조흥연;양한철
    • Microbiology and Biotechnology Letters
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    • v.21 no.6
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    • pp.622-627
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    • 1993
  • Rhodospirillum rubrum P17 was used to investigate the pontential for the treatment of soybean curd waste and for the utilization of the biomass produced. The maximal biomass production and COD removal from the waste water were obtained at 30C, pH 7.0 under 2,500lux production and 50 rpm of agitation. The initial COD level of the soybean curd waste water was 3,240mg/l, and after 4 days of cultivation in batch culture, 3.46g/l of cells was obtained and COD level of the waste water reduced to 150mg/l (COD removal rate 95.4%).

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Improved Performance of Microbial Fuel Cell Using Membrane-Electrode Assembly

  • PHAM THE HAl;JANG JAE KYUNG;MOON HYUN SOO;CHANG IN SEOP;KIM BYUNG HONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.438-441
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    • 2005
  • A mediator-less microbial fuel cell (MFC) was used to determine the performance effects of a membrane­electrode assembly (MEA). The MFC with an MEA generated a higher current with an increased coulomb yield when compared to an MFC with a separate cathode. Less oxygen was diffused through an MEA than through a Nafion membrane. The MFC performance was improved with a buffer, although a high-strength buffer reduced the performance.

Relationship between the Organic Content and the Number of Sdphate-Reducing Bacteria in the Tributaries to the Han River (한강 지류의 유기물 농도와 황산염 환원세균의 상관관계)

  • 김해영;김태성;김병홍;김재문
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.233-235
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    • 1992
  • The number of sulphate-reducing bacteria (SRB) in some of the tributaries to the Han River was determined by the most probable number method using Postgate's Medium E.Higher number of SRB were obtained in the streams to which industrial waste water is discharged than those receiving only domestic waste water.

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Occurrence and Molecular Differentiation of Environmental Mycobacteria in Surface Waters

  • Lee, Eun-Sook;Lee, Mok-Young;Han, Sun-Hee;Ka, Jong-Ok
    • Journal of Microbiology and Biotechnology
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    • v.18 no.7
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    • pp.1207-1215
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    • 2008
  • To investigate the occurrence and species diversity of mycobacteria in waters, surface water samples were collected monthly from the Han River and tap water samples at the terminal sites of the distribution system. Mycobacteria in each water sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Middlebrook 7H10 agar, and then identified by polymerase chain reaction-restriction fragment length polymorphism analysis (PRA) and sequencing of the 65-kDa heat-shock protein gene (hsp65 gene). Mycobacteria were detected in 59% of the surface water samples and 26% of the tap water samples. Over half of the 158 isolates could not be identified by hsp65 PRA and gene sequencing, and several identification discrepancies were observed between the two methods. The most frequently isolated species was Mycobacterium gordonae in surface water and M. lentiflavum in tap water. M. avium complex (MAC), the most important pathogen among environmental mycobacteria, was detected in the surface water samples but not found in the tap water samples. The result demonstrated that water is an important environmental source of mycobacteria and the combined application of hsp65 PRA and sequencing was more reliable than hsp65 PRA alone to accurately identify mycobacteria present in water.

Bacterial Mixture from Greenhouse Soil as a Biocontrol Agent Against Root-Knot Nematode, Meloidogyne incognita, on Oriental Melon

  • Seo, Byoung-Joo;Kumar, V.J. Rejish;Ahmad, Rather Irfan;Kim, Byung-Chun;Park, Wan;Park, So-Deuk;Kim, Se-Eun;Kim, Sang-Dal;Lim, Jeong-Heui;Park, Yong-Ha
    • Journal of Microbiology and Biotechnology
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    • v.22 no.1
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    • pp.114-117
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    • 2012
  • The biological control efficacy of a greenhouse soil bacterial mixture of Lactobacillus farraginis, Bacillus cereus, and Bacillus thuringiensis strains with antinematode activity was evaluated against the root-knot nematode Meloidogyne incognita. Two control groups planted in soil drenched with sterile distilled water or treated with the broad-spectrum carbamate pesticide carbofuran were used for comparison. The results suggest that the bacterial mixture is effective as a biocontrol agent against the root-knot nematode.

Electron Microscopic Study for the Influence of Soaking in Hot Water and Prochloraz Solution on Spore and Mycelium of Fusarium fujikuroi Infected in Rice Seed (온탕소독과 prochloraz 침지소독이 벼 종자에 감염된 Fusarium fujikuroi의 포자와 균사의 형태에 미치는 영향에 대한 전자현미경적 연구)

  • Park, Woo-Sik;Yeh, Wan-Hae;Lee, Se-Weon;Han, Seong-Suk;Lee, Jun-Seong;Lim, Chun-Keun;Lee, Yong-Hwan
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.176-181
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    • 2008
  • This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.