• Title/Summary/Keyword: Water lettuce

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Relationship Between Relative Water Content and Ascorbate Redox Enzymes Activity in Lettuce Leaves Subjected to Soil Water Stress (토양 수분 Stress에 따른 상추의 엽중 상대수분 함량과 아스코브산 관련 효소 활성도)

  • Kang, Sang-Jae;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.32-39
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    • 2013
  • The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

Evaluation of Environmental Toxicities for Priority Water Pollutants in a Small Watershed by Bioassays - Comparision between Lettuce Seed Germination Test and Microtox Bioassay - (생물학적 검정법을 이용한 소규모 수계내 수질 오염물질의 환경독성 평가 -상추씨 발아시험과 Microtox 시험 비교-)

  • 이지나;황인영
    • Environmental Analysis Health and Toxicology
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    • v.14 no.4
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    • pp.135-144
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    • 1999
  • Environmental toxicities of priority water pollutants were evaluated by two selected bioassays, Lettuce seed germination/elongation test and Microtox acute toxicity test. Toxic chemicals (heavy metals, polycyclic aromatic hydrocarbons, and phenolic compounds) inhibited the germination rate and root elongation of Lettuce seed, as well as the bioluminescence of Microtox bacteria. When test biota were exposed to target chemicals, the sensitivity of Lettuce bioassay was relatively lower than that of Microtox bioassay. However, Lettuce bioassay may be a good candidate for prescreening the environmental toxicities of priority water pollutants, since the testing method with Lettuce seed was relatively easier and more economic than with Microtox bacteria. Toxicity tests were conducted to compare the validity and sensitivity of both bioassays for sediment from a small stream passed through urban area as well as leachate from a municipal solid waste landfill. From experimental results, we found that Lettuce test and Microtox test are compensated each other as a battery of bioassay for evaluating the environmental toxicities of field samples obtained from a small stream contaminated by pollutants.

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Antioxidant Potential and Chlorogenic Acid Level of Lettuce (Lactuca sativa L.) Cultivars

  • Chon Sang-Uk
    • Plant Resources
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    • v.8 no.2
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    • pp.103-109
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    • 2005
  • Lettuce (Lactuca sativa L.) is known to contain water-soluble substances that improve antioxidant status due to the richness in antioxidants. Greenhouse experiment was carried out under different shading conditions during spring lettuce growing season. Shade significantly reduced shoot weight, number of leaves and chlorophyll content, while it increased shoot length of lettuce plants. The antioxidant potential of the individual fraction was in order of n-butanol > ethyl acetate > water > n-hexane fraction, although was less than that of commonly used antioxidants, BHT and ascorbic acid. Fractions from lettuce plants dose-dependently increased DPPH free radical scavenging activity, in vitro test. By means of HPLC analysis, BuOH fraction of cultivar 'Hwahyang' (57.93 mg $100g^{-1}$) had the highest amount of antioxidant chi orogenic acid. Shading treatment increased average amount of chlorogenic acid of all cultivars in BuoH, EtOAc, hexane and water fractions by 33, 120, 144, and $58\%$, respectively. These results suggest that lettuce plants had potent antioxidant activity, and their activities were differently exhibited depending on cultivar and fraction.

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Microbial Removal Effects of Electrolyzed Acid Water on Lettuce by Washing Methods and Quality Changes during Storage (전해산화수에 의한 상치의 세척방법별 제균효과와 저장중 품질변화)

  • Jeong, Seong-Weon;Jeong, Jin-Woong;Park, Kee-Jai
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1511-1517
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    • 1999
  • Effects of washing methods using electrolyzed acid water on lettuce(Lactuca sativa) and quality changes during storage were investigated. The multi-stage immersion treated 3 times in 2 min showed more effective than others to remove microorganisms. Total count of lettuce after immersion in electrolyzed acid water was decreased to 1/100-1/300 of $5.8\;{\times}\;l0^5CFU/g$ of non-immersed lettuce and $2.3\;{\times}\;l0^5CFU/g$ of tap water immersed lettuce. Also coliforms was significantly decreased to 1/3,000 of $3.1\;{\times}\;10^3CFU/g$ after electrolyzed acid water washing. However, microbial levels of electrolyzed acid water treated one became to be similar to those of non-treated lettuce after 3 days of storage at $10^{\circ}C$. The color values of L and b of lettuce treated with electrolyzed acid water were somewhat higher than those of others. Though chlorophyll content of lettuce just after immersion in electrolyzed acid water was 9% lower than those of non-treated one, the content was decreased to the same level of other treatments during storage. Decaying ratio showed the lowest value in lettuce immersed in electrolyzed acid water until 6 days of storage. The sensory tests for overall acceptability and appearance of lettuce immersed in electrolyzed acid water showed higher than those of others until $3{\sim}6$ days of storage.

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Inorganic Nutrient Removal Efficiency of Aquatic Plants from Recirculating Aquaculture System (수생식물을 이용한 담수 순환여과식 양식용수내의 무기영양염 처리 효율)

  • 마진석;오승용;조재윤
    • Journal of Aquaculture
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    • v.16 no.3
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    • pp.171-178
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    • 2003
  • Inorganic nutrients such as nitrogen and phosphate compounds accumulate in recirculating aquaculture systems. These nutrients must be removed from the system before they affect pH and fish health. For this purpose, aquatic plants are a simple and inexpensive method of removal. There are four commonly used aquatic plants: Eichhornia crassipes (water hyacinth), Pistia stratiotes (water lettuce), Hygrophila angustifolia, and Hydrocotyle leucocephala in freshwater recirculating aquaculture systems in Korea, but their efficiencies are not known. Therefore, removal efficiencies of inorganic nutrients from a freshwater recirculating aquaculture water with four commonly used aquatic plants were tested. Removing efficiencies of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N of the plants in 210 L aquaria for 48-hour period were tested. The removing efficiencies of TAN, N $O_3$$^{[-10]}$ -N, and P $O_4$$^{3-}$-P of the two most effective plants, water hyacinth and water lettuce, were also tested in 690 L (surface area of 1.55 $m^2$) tanks under 2 different initial stocking densities, 4 kg and 6 kg, for 22 days. Proximate analysis major nutrients and N and P contents of the all plants were analyzed for calculating net removal weight of N and P by the plants. Water lettuce was the most effective for removing TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N from the water for 48-hour period tested followed by water hyacinth and Hygrophila angustifolia. Water lettuce reduced TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N concentration from 2.3 mg/L, 0.197 mg/L, and 21.4 mg/L to 0.4 mg/L, 0.024 mg/L and 17.4 mg/L, respectively while water hyacinth reduced them down to 0.6 mg/L, 0.029 mg/L and 17.9 mg/L, respectively. The concentrations of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N in Hydrocotyle leucocephala group were rather increased up to 3.7 mg/L, 5.7 mg/L and 48.2 mg/L, respectively. This is because the creeping stem of Hydrocotyle leucocephala had to be cut to meet stocking weight resulting in decaying of the stem in the aquaria during experiment. The net growth in weight of water hycinth and water lettuce of 4 kg each in the 1.55 $m^2$ tanks for 22 days were 9.768 kg and 10.803 kg respectively, and those at initial weight of 6 kg each were 8.393 kg and 9.433 kg, respectively. The reason of lower net growth in the later group was restricted growth space. Nitrogen and phosphorus contents in water hyacinth were 2.89% and 0.27%, and those in water lettuce were 3.87% and 0.36%, respectively. Average quantities of removed N and P from 1.55 $m^2$ tanks by water hyacinth for 22 days were 18.9 g and 1.75 g, while those by water lettuce were 36.8 g and 3.5 g, respectively. Therefore water lettuce showed much higher efficiencies for removing both N and P from recirculating aquaculture water than water hyacinth.

Effect of Chemical Treatment with Citric Acid or Ozonated Water on Microbial Growth and Polyphenoloxidase Activity in Lettuce and Cabbage

  • Youm, Hyoung-Jun;Jang, Jae-Won;Kim, Kyu-Ri;Kim, Hyo--Jjung;Jeon, Eun-Hee;Park, Eun-Kyoung;Kim, Mee-Ree;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • v.9 no.2
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    • pp.121-125
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    • 2004
  • Effects of chemical treatment with a citric acid solution or ozonated water on microbiological changes in lettuce and cabbage during storage were studied. Fresh lettuce and cabbage samples were cut into small pieces and treated by soaking in either ozonated water or a citric acid solution. After treatment, populations of total bacteria, yeast and mold, and E. coli were determined. Numbers of microorganisms increased during storage, but ozonated water and citric acid treatments retarded the increase in microbial growth. Among treatments, 1 % citric acid treatment was the most effective in terms of microbiological change and inhibition of polyphenoloxidase (PPO). For lettuce, citric acid treatment decreased the microbial growth overall by 1.5 log CFU/g and inhibited the PPO activity by 80%. These results indicate that chemical-treated lettuce and cabbage retained a better quality than those of the control during storage.

Study on the Lettuce Growth Using Different Water Sources in a Hydroponic System (수경재배용 용수 종류에 따른 상추 생장 연구)

  • Heo, Jeong Min;Kim, Ga Eun;Kim, Jin Hwang;Choi, Byeongwook;Lee, Sungjong;Lee, Byungsun;Jho, Eun Hea
    • Korean Journal of Environmental Agriculture
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    • v.41 no.3
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    • pp.191-198
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    • 2022
  • BACKGROUND: Plants can be grown using a culture medium without soil using a hydroponic system. Crop production by the hydroponic system is likely to increase as a means of solving various problems in the agricultural sector such as aging of rural population and climate change. Different water sources can be used to prepare the culture medium used in the hydroponic system. Therefore, it is necessary to study the effect of different water sources on crop production by the hydroponic system in order to explore the applicability of various water resources. METHODS AND RESULTS: Lettuce was cultivated by the hydroponic system and three different water sources [tap water (TW), bottled water (BW), and groundwater (GW)] were used to compare the effect of water sources on lettuce growth. The three kinds of waters with a nutrient solution (TW-M, BW-M, GW-M) were also used as the media. After the six-week growth period, the lettuce length and weight, the number of leaves, and the contents of chlorophylls and polyphenols were compared among the different media used. The lettuces did not grow in the waters without the nutrient solution. In the media, the lettuce growth and the contents of chlorophylls were affected by the different water sources used to prepare the media, while the contents of polyphenols were not affected. The absorbed amounts of ions by lettuces, especially Ca and Zn ions, and the dry weight of the harvested lettuces showed a strong positive correlation. CONCLUSION(S): Overall, this study shows that different water sources used for growing lettuce in a hydroponic system can affect lettuce growth. Further studies on the enhancement of crop qualities using different water sources may be required in future studies.

Sensory and textural characteristics of wageobyung using varied levels of lettuce and different amount of sugars and water (와거병의 재료배합비에 따른 관능적ㆍ텍스쳐 특성)

  • 이효지;손희선
    • Korean journal of food and cookery science
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    • v.19 no.2
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    • pp.181-187
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    • 2003
  • The objective of this study was to investigate by a sensory evaluation and mechanical examination the sensory and quality characteristics of wageobyune containing different ratios of ingredients such as lettuce (50%, 60% and 70%), sugar and water. The results of the sensory evaluation showed that wageobyung containing 50% lettuce had higher scores in the overall acceptability, color, flavor and chewiness preference. In the textural analysis of wageobyung, the springiness, cohesiveness and hardness were the highest and the chewiness, gumminess and adhesiveness were the highest in the 70% added samples. Increasing the lettuce content decreased the hunter's color L-value of wageobyung. The redness and yellowness of wageobyung were increased as more lettuce was added. The moisture content of wageobyung was higher in the samples containing 70% lettuce than those containing 50%.

Effects of Saline Irrigation Water on Lettuce and Carrot Growth in Protected Cultivation (관개용수 염도수준에 따른 시설 상추 및 당근의 생육 영향 분석)

  • Jeon, Jihye;Jeong, Hanseok;Kim, Hakkwan
    • Journal of The Korean Society of Agricultural Engineers
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    • v.57 no.4
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    • pp.113-120
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    • 2015
  • The objectives of this study were to monitor and assess the effects of saline irrigation water on lettuce and carrot growth in protected cultivation. One control and 4 treatments with three replications, which were differentiated according to the level of salinity in irrigated water, were employed for each vegetable to assess the effects of the irrigation with saline water. Monitoring results showed that the use of irrigation water containing above a certain level of salinity was found to cause excessive accumulation of salts in the soil as saline irrigation water increased electrical conductivity (EC) and sodium ($Na^+$) content in both lettuce and carrot soil samples, while tap water irrigation used as control decreased the salinity in the samples. The salinity higher than the threshold level of irrigation water was found to reduce the yields of lettuce and carrot, while in less than the threshold level the higher the salinity of the irrigation water increased the yields. The salinity of the irrigation water also appeared to increase the internal salinity of the plant as the $Na^+$ content in plant increased as the salinity of irrigation water increase. Increased $Na^+$ content was analyzed to be able to increase the sugar content in carrot. This study could contribute to suggest water quality criteria for safe use of saline water in protected cultivation, although long-term monitoring is needed to get more representative results.

Effect of Soil Salinity on Nitrate Accumulation of Lettuce (토양 염류집적이 상추의 Nitrate 축적에 미치는 영향)

  • Jin, Sun-Jae;Cho, Hyun-Jong;Chung, Jong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.2
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    • pp.91-96
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    • 2004
  • Accumulation of nitrate in edible crops is undesirable due to potential risks to human health. Since nitrate has a role in the osmotic regulation of plants, salt accumulation in soil is expected to stimulate nitrate accumulation in plants. Lettuce (Lactuca sativa L.) was grown in soils of different salinities, 9.69 and $4.49dS\;m^{-1}$, in a greenhouse, and the effect of soil salinity on nitrate accumulation in lettuce was investigated. Content of nitrate in the lettuce increased significantly as soil salinity increased under low light intensity and ample supply of nitrate in root media. Soluble sugar and oxalate contents in lettuce were also significantly higher in the soil of higher salinity. Phosphate, Cl, and $SO_4$ contents in lettuce were not significantly different in soils of different salinities. Among the cations, K content in lettuce was significantly higher in the soil of higher salinity, but Na, Ca, and Mg comtents were not much influenced. Comparing to the lettuce grown in low salinity soil, although the growth of lettuce was decreased by 9% in the soil of higher salinity, nitrate accumulation in the lettuce was increased by 18.6%. These results indicate that higher nitrate content in lettuce of higher salinity soil is a positive accumulation to adapt to the water stress condition. The nitrate accumulation of vegetables grown in plastic film houses is known to be due to the heavy fertilization and low light intensity, but salt accumulation in the soil, which can lower soil water potential, is expected to stimulate the nitrate accumulation further.