• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.365-373
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• 2020

We previously reported the potential of Senna tora L. seeds fermented by Lactobacillus casei (FSL) as a laxative agent in a loperamide-induced constipation rat model. Here, we examine the mechanism of action of FSL and its bioactive compound, revealed herein, on loperamide-induced constipation Sprague Dawley rat model. We identified the compound aurantio-obtusin (AO) using HPLC quantitative analysis. Rats were randomly assigned to six experimental groups (eight rats each)-normal and constipated groups (loperamide, FSL [100, 300, 500 mg/kg], and AO [1 mg/kg]). The FSL and AO-treated group showed an increase in the frequency, amount, and water content of feces in the constipated rat. Moreover, FSL and AO increased the intestinal transit speed in the constipated rat. Histological analysis revealed that FSL and AO recovered the intestinal mucus, the number of goblet cells, as well as thickness of the mucosa layer and muscle. Furthermore, the protein levels of the muscarinic acetylcholine receptor M3, which is involved in intestine contraction, were recovered in the FSL and AO-treated group. Its downstream signaling pathway (p-protein kinase C) was recovered by FSL and AO treatment. In conclusion, fermentation of S. tora L. seeds increases AO, which improves intestinal function, indicating that FSL is effective for treating constipation.

• 대한건축학회논문집:계획계
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• 제34권6호
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• pp.93-103
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• 2018

In the past, the rooftop was a place where a water tank was to be installed, and a space of secretion and concealment such as a warehouse exposed in the air. It was because it was judged to hinder the beauty of a city as a whole without having any functional value per se. However, the roof portion is increasingly being utilized for a space such as a cafe, a pub, and a lounge as the rooftop is highly recognized as a place for leisure activities other than an idle space in modern times. Nonetheless, there are no detailed ordinances and criteria corresponding to such circumstances. For example, in case of the rooftop space, the building code applied to the rooftop space is a legal system when the rooftop was an idle space in the past though people have now accessed more increasingly to the rooftop than in the past. This study investigated the definition on the rooftop space evolved in accordance with the change of the rooftop space and the related legal system in Korea, and conducted a survey for the purpose of performing the field investigation for the analysis of physical status in 117 spaces that form the rooftop space in the rooftop of small-scale commercial facilities with 2 stories through 4 stories in Korea and analyze the recognition of users and analyzing the recognition of users. We analyzed the problems in the rooftop space through analysis of derived data, and suggested improvement plans.

• 문화기술의 융합
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• 제7권1호
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• pp.256-263
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• 2021

본 논문은 정부 부동산 정책의 문제점과 개선방안을 연구하였다. 문재인 정부는 세금 중과로 투기 세력을 잡겠다고 하며 규제 일변도로 나아가고 있다. 공급보다는 재건축 규제나 은행 대출을 강화했고 양도소득세도 인상했다. 정부가 경제보다 정치 논리를 강조하며 대책을 시행하자 시장은 불안하고 경기는 침체에 빠지고 있다. 토지는 인구증가라든지 산업화, 도시화, 부의 증대로 인하여 문제의 악순환을 증가시켜왔다. 부동산 문제는 토지정책을 잘못 수립하면 토지가격 상승뿐만 아니라 무질서한 국토이용이 가속화되고 거래 질서의 문란이 파생된다. 또한 부동산은 한번 엎지른 물은 담기 어려울 정도로 토지문제를 회복하기 어렵다. 이것을 토지의 비가역성이라고 한다. 지가가 한번 폭등하면 다시 잡기 어렵고 무분별한 개발은 생태계의 파괴로 이어지어 되돌기가 여간 쉽지 않다. 이렇게 복잡다단한 부동산 문제을 정부 정책으로 단기간에 단시일에 벌주듯 시행해서는 안 되는 이유다. 이번 논문은 부동산정책의 문제점을 고찰하고 이에 대한 개선방안을 살펴보고자 한다.

• 대한건축학회논문집:구조계
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• 제34권2호
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• pp.75-82
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• 2018

Green roof in Korea has been recognized as part of landscape area and local governments enacted municipal ordinance for supporting green roof thereby assisting part of expenses spent for green roof within a range of budget for buildings which are eligible for the green roof support of existing buildings. However, it is necessary to enforce a regulation of mandatory green roof for new buildings in order to expand green roof application and provide a variety of assistances such as subsidy payment, tax relief, and mitigation of floor area ratio-related regulation as done in other advanced nations. In particular, LEED(Leadership in Environmental and Energy Design) in the USA applies additional point directly for green roof in terms of the reduction in heat island effects. For example, the following items are beneficial to have additional points: habitat preservation or restoration, external space, rainwater management, reduction in outdoor water usage, minimum energy performance, and optimum energy performance. In addition, the BREEAM(Building Research Establishment Environmental Assessment Method) in the UK specifies green roof as one of assessment items to be complied in terms of diversity of species. As such, LEED and BREEAM reflect direct and indirect effects of green roof on assessment criteria, which suggest a direction to green building certification criteria in Korea where only additional points are given for green roof according to soil depth.

• 한국지반환경공학회 논문집
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• 제12권10호
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• pp.5-12
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• 2011

최근 국내에서는 지반개량 재료로 무기질계 급결재와 초미립자 시멘트를 주원료로 하는 NDS(Natural and Durable Stabilizer)공법 등의 개발이 활발하게 연구되어 왔다. 하지만 기존의 연구에서는 NDS의 재령일별 강도발현 과정에 있어서의 화학적인 변화과정 및 강도발현의 원리를 설명해 주지는 못하고 있는 실정이었다. 따라서 무기질계 주입재의 대중화를 위해서는 경화과정의 메커니즘 규명이 확실하게 선행되어야 한다고 판단하였고, 일축압축시험, SEM분석, XRD분석을 재령일 별로 실시하여 각각의 결과값을 분석하였다. 또한 그 특징을 더욱 분명히 구별하기 위하여 물유리계 주입재의 대표적인 예인 SGR 또한 동일한 시험을 실시하여 비교대상으로 하였다. 시험결과 NDS의 강도발현 메커니즘을 일축압축강도-SEM-XRD의 유기적 상관성을 통해 도출할 수 있었고 그 성능의 우수함을 확인하였다.

• 한국지반환경공학회 논문집
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• 제12권11호
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• pp.13-21
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• 2011

하천의 기본기능인 치수, 이수, 환경 및 문화 등을 되살리기 위한 국가적 사업인 4대강 국가하천 정비사업은 홍수에 안전한 문화 및 생태가 살아있는 친환경 하천 공간을 정비하기 위한 것으로 건설재료의 친환경성이 강조되고 있다. 본 연구에서는 다당류 친환경 개량재인 콘알파를 활용한 토양 및 시멘트 혼화제를 개발하여 다짐시험, 투수시험 및 일축압축강도시험을 통하여 친환경 하천 공간을 위한 건설재료로의 적합성을 평가하고자 하였다. 일축압축시험의 결과로는 다당류 지반개량재가 강도 증진에 큰 영향을 끼치지 않음을 알 수 있다. 이에 대한 원인을 분석한 결과, 일반적으로 자연 상태에서 토사에 서식하고 있는 각종 균들이 본 연구에서 정확한 배합비를 얻기 위해 건조로에서 고온으로 건조를 시키는 과정에서 활동성이 극 저하되어 강도증진 효과에 반영되지 않은 것으로 나타났다. 향후, 다당류 친환경 지반개량재와 관련된 시험에서는 각 시료를 자연상태에서 함수비를 측정하여 적정함 수비로 다진 뒤, 다당류 지반개량재를 혼합하여 공학적 시험을 실시하는 것이 바람직할 것으로 사료된다.

• 수산해양기술연구
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• 제57권3호
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• pp.194-204
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• 2021

The research was analyzed the catch data of the five years (2016-2020) for six joint venture company tuna purse seiners in the Atlantic Ocean, with the aim of suggesting improvement measures for responsible and sustainable fishing according to changes of recommendation by International Commission for the Conservation of Atlantic Tuna (ICCAT) on the tropical tunas. In the last five years, the average catch of six tuna purse seiner gradually increased to 7,745 tons, 8,364 tons and 9,053 tons from 2016 to 2018, but decreased to 7,761 tons in 2019 and 6,214 tons in 2020. The reason for the decrease in fishing volume in 2019 and 2020 was the fluctuation of the formation of the cold water zone (22-23℃), and the total ICCAT convention area of FAD closure in January and February due to the expansion of the FAD closure area and poor free school catching during two months period, respectively. The analysis on fishing area showed that the percentage of fishing in the high sea was about 85% although the FAD closure area included the EEZ zone in coastal countries; the rise of the fishing license in coastal countries is also believed to be a factor. In order to overcome such situations and improve catching volume, it will be possible if excellent manpower is secured, school fishing is expanded, and the production of high value-added catch (purse seine special: PS).

• 한국축산식품학회지
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• 제41권5호
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• pp.869-882
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• 2021

Sodium chloride (NaCl) replacement with calcium chloride (CaCl₂) effect on protein solubility, proteolytic enzyme and quality characteristics of a chicken soup prepared from spent hen (SH) chicken were investigated. By means of immerse marination prior to cooking, a total of 60 skinless SH breast meat were randomly allocated into ten groups admitted to treatments with marinade solution containing sodium tripolyphosphate (STPP) and reduced percentage of NaCl with CaCl₂ at 0%, 25%, 50%, 75%, and 100% at 4±2℃ for 20 h. STPP was adjusted to 0.5% for all treatments and NaCl replacement at 0% was used as control. The different methods, particularly boiling at 100℃ and retorting at 121℃, 1.5 kgf/cm² for 60 minutes, were applied following marination. An upregulation of cathepsin-B and caspase-3 enzymes were a consequences from a higher percentage of CaCl₂ within meat environment. Accordingly, modified the protein solubility in particular the myofibrillar and total protein solubility. In addition, a significant increase in water holding capacity (WHC), pH value, myofibril fragmentation index (MFI), and moisture content was obtained due to salt replacement (p<0.05). Limited effect was observed for shear force value, collagen content and cooking yield. Eventually, this study implied that although protelytic enzyme and protein solubility was upregulated by the replacement of NaCl with CaCl₂ at >75%, extensive effect on texture properties was not observed. Therefore, NaCl replacement at 75% could be a promising strategy for quality improvement of SH chicken soup.

• 동의생리병리학회지
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• 제35권5호
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• pp.169-176
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• 2021

This study was conducted to suggest the Cudrania tricuspidata leaf and Achyranthes japonica Nakai Complex (CAC) possibility of use as a functional natural material for improving bone disease. Cudrania tricuspidata leaf and Achyranthes japonica Nakai were mixed in the same amount, extracted with hot water, and then powdered and used in the study. After, the cytotoxicity of CAC for osteoblasts (MG63 cell), osteoclasts (differentiated RAW264.7 cell), and macrophages (RAW264.7 cell) were evaluated by MTT assay, and ALP assay and TRAP assay were performed to confirm the differentiation capacity of osteoblasts and osteoclasts, respectively. In addition, the anti-inflammatory effect in macrophages was evaluated by ELISA, qRT-PCR, and western blot assay. CAC did not proliferated osteoblasts and osteoclasts, but increased ALP activity against osteoblasts differentiation and decreased TRAP activity against osteoclasts differentiation. CAC did not proliferated macrophages but decreased nitric oxide production. Also, decreased NOS2, IL1B, IL6, PTGS2, and TNFA gene expression, and JNK and p38 protein phosphorylation in a concentration-dependent manner, but ERK protein phosphorylation was not changed. As a result, CAC increased the differentiation and activation of osteoblasts, inhibited the differentiation and activation of osteoclasts, and regulated the expression of inflammatory cytokines in macrophages. Therefore, it is thought that CAC can be used as a functional natural material that prevents bone disease and has an anti-inflammatory effect.

• Nutrition Research and Practice
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• 제16권2호
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• pp.173-193
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• 2022

BACKGROUND/OBJECTIVES: Alzheimer's disease (AD) is one of the most representative neurodegenerative disease mainly caused by the excessive production of amyloid beta (Aβ). Several studies on the antioxidant activity and protective effects of Populus tomentiglandulosa (PT) against cerebral ischemia-induced neuronal damage have been reported. Based on this background, the present study investigated the protective effects of PT against cognitive impairment in AD. MATERIALS/METHODS: We orally administered PT (50 and 100 mg/kg/day) for 14 days in an Aβ_25-35-induced mouse model and conducted behavioral experiments to test cognitive ability. In addition, we evaluated the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and measured the production of lipid peroxide, nitric oxide (NO), and reactive oxygen species (ROS) in tissues. RESULTS: PT treatment improved the space perceptive ability in the T-maze test, object cognitive ability in the novel object recognition test, and spatial learning/long-term memory in the Morris water-maze test. Moreover, the levels of AST and ALT were not significantly different among the groups, indicating that PT did not show liver toxicity. Furthermore, administration of PT significantly inhibited the production of lipid peroxide, NO, and ROS in the brain, liver, and kidney, suggesting that PT protected against oxidative stress. CONCLUSIONS: Our study demonstrated that administration of PT improved Aβ_25-35-induced cognitive impairment by regulating oxidative stress. Therefore, we propose that PT could be used as a natural agent for AD improvement.