• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.5
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• pp.857-862
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• 2011

The purpose of this study is to investigate the anti-proliferative mechanism by Trichosanthes kirilowii (TCK) in MCF-7 human breast carcinoma cell. In this study, we used human breast cancer cell line, Michigan cancer foundation-7 cells (MCF-7 cells). They were co-incubated with 30~200 ${\mu}g$/ml TCK for 48 hours, and cell viability was measured by Water-soluble tetrazolium salt-1 (WST-1) assay. After MCF-7 cells were exposed to 60 ${\mu}g$/ml of TCK for 0, 3, 6, 12, 24, 48 hours, We performed flow analysis cytometry sorting(FACS) and western blot analysis. We investigated the effect of dose-dependent cell growth inhibition by TCK, which could be proved by WST-1 assay. Also, flow cytometry analysis showed that TCK increased percentage of subG1 phase and G2/M phase cell cycle. In addition, TCK induced apoptosis through the expression of caspase-9, -3 and poly(ADP-ribose) polymerase(PARP) activation. Moreover, we showed that ATM-dependent G2/M phase arrest by DNA damage and phosphorylation of chk2, cdc25C, cdc2(Tyr15). Taken together, these results suggest that by G2/M phase arrest through DNA damage and inducing of apoptosis through intrinsic pathway, TCK may have potential tumor suppressor in breast cancer.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.363-368
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• 2016

We describe here the mass death of predatory carp, Chanodichthys erythropterus, in Korea induced by plerocercoid larvae of Ligula intestinalis as a result of host manipulation. The carcasses of fish with ligulid larvae were first found in the river-edge areas of Chilgok-bo in Nakdong-gang (River), Korea at early February 2016. This ecological phenomena also occurred in the adjacent areas of 3 dams of Nakdong-gang, i.e., Gangjeong-bo, Dalseong-bo, and Hapcheon-Changnyeong-bo. Total 1,173 fish carcasses were collected from the 4 regions. To examine the cause of death, we captured 10 wondering carp in the river-edge areas of Hapcheon-Changnyeong-bo with a landing net. They were 24.0-28.5 cm in length and 147-257 g in weight, and had 2-11 plerocercoid larvae in the abdominal cavity. Their digestive organs were slender and empty, and reproductive organs were not observed at all. The plerocercoid larvae occupied almost all spaces of the abdominal cavity under the air bladders. The proportion of larvae per fish was 14.6-32.1% of body weight. The larvae were ivory-white, 21.5-63.0 cm long, and 6.0-13.8 g in weight. We suggest that the preference for the river-edge in infected fish during winter is a modified behavioral response by host manipulation of the tapeworm larvae. The life cycle of this tapeworm seems to be successfully continued as the infected fish can be easily eaten by avian definitive hosts.

• Journal of Soil and Groundwater Environment
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• v.18 no.3
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• pp.109-118
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• 2013

Characteristics of the transport of zero-valent iron nanoparticles (nZVI) in an aquifer were investigated to evaluate an application of nZVI-based reactive zone technology. Main flow direction of groundwater was north. Preferential flow paths of the groundwater identified by natural gradient tracer test were shown northeast and northwest. The highest groundwater velocity was $4.86{\times}10^{-5}$ m/s toward northwest. When the breakthrough curves obtained from the gravity injection of nZVI were compared with the tracer curves, the transport of nZVI was retarded and retardation factors were 1.17 and 1.34 at monitoring wells located on the northeast and northwest, respectively. The ratios of the amount of nZVI delivered to the amount of tracer delivered at the two wells mentioned above were 24 and 28 times greater than that of the well on the main flow direction, respectively. Attachment efficiency based on a filtration theory was $4.08{\times}10^{-2}$ along the northwest direction that was the main migration route of nZVI. Our results, compared to attachment efficiencies obtained in other studies, demonstrate that the mobility of nZVI was higher than that of results reported in previous studies, regardless of large iron particle sizes of the current study. Based on distribution of nZVI estimated by the attachment efficiency, it was found that nZVI present within 1.05 m from injection well could remove 99% of TCE within 6 months.

• Journal of Wetlands Research
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• v.13 no.3
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• pp.471-479
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• 2011

Evapotranspiration (ET) including evaporation from a land surface and transpiration from photosynthesis of vegetation is a hydrological factor that has an important role in water cycle. However, there is a limitation to understand it due to heterogeneity of land cover and vegetation. In this study, Mapping EvapoTRanspiration with Internalized Calibration (METRIC) model, one of the energy balance models, and MODerate resolution Imaging Spectroradiometer (MODIS) satellite based well-known Penman-Monteith algorithm were compared. Two ET maps were categorized and compared by land cover classification. The results represented overall applicability of the two models with the highest correlation coefficients in needleleaf and broadleaf forests. This study will be useful to estimate remote sensing based ET maps with high resolution and to figure out spatio-temporal variability and seasonal changes.

• Membrane Journal
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• v.28 no.1
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• pp.37-44
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• 2018

Polysulfone composites including graphene were prepared, and their thermal characteristics in membrane states were analyzed by using a custome-made residual stress analyzer and a thermal diffusivity analyzer based on laser flash method. The residual stress analysis was carried out on the polysulfone composite films deposited on Si (100) substrates for 1 cycle of heating and cooling runs. The flat membrane of graphene-embedded polysulfone composites were prepared by the phase transfer method in distilled water and the thermal conductivity was separately measured in the out-of-plane and the in-plane directions. The residual stress of the graphene-embedded polysulfone film was gradually decreased with increasing graphene loading and the out-of-plane thermal conductivity was distinguished from the in-plane thermal conductivity in the flat membranes. These thermal characteristics are caused by the structural uniqueness of graphene and the micro-void structures formed during membrane fabrication.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.297-309
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• 1990

Ginseng is renowned for both its medicinal and herbal uses and successful cultivation of Panax ginseng in Asia and Panax Vtiinvtiefolilim in North America has until recently taken place in the native geographical ranges of the plants. As a consequence of the potential high capital return and anticipated increases in consumer consumption, commercial cultivation of American ginseng now occurs well outside the native range of the plant in North America. In fact, the region of greatest expansion of cultivation is in the semi-arid interior region of British Columbia, Canada. Linked with this expansion is the potential domination of the ginseng industry by agricultural corporations. In the interior of British Columbia, the native decidous forest environment of eastern North America is simulated with elevated polypropylene shade and a sllrface covering of straw mulch. The architecture of these environments is designed to permit maximillm machinery useage and to minimize labour requirements. Further, with only a four-year growth cycle, plant densities in the gardens are high. In this hot, semiarid environment, producers believe they have a competitive advantage over other regions in North America because of the low precipitation rates. This helps to minimize atmospheric humidity such that the conditions for fungal disease development are reduced. If soil moisture levels become limited, supplemental water can be provided by irrigation. The nature of the radiation and energy balance regimes of the shade and much environment promotes high soil moistilre levels. Also, the modified environment reduces soil heating. This can result in an aerial environment for the plant that is stressful and a rooting zone environment that is sub-optimal. The challenge of further refining the man modified environment for enhanced plant growth and health still remains.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.9
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• pp.4045-4052
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• 2012

The aim of this single blind intervation study was to compare the analgesic effects of transcutaneous electrical nerve stimulation (TENS) and interferential currents (IFC) on cold-induced pain in healthy volunteers. Sixteen subjects completed six cycles of the cold-induced pain test. During each cycle pain threshold was recorded as the time from immersion of the subject is hand in cold water to the first sensation of pain and pain intensity and unpleasantness ratings were recorded using visual analogue scales. Subjects were randomly allocated to receive each 50 Hz-TENS, 50 Hz-IFC, 100 Hz-TENS and 100 Hz-IFC. Statistical analysis showed that four interventions elevated the cold pain threshold significantly and the difference between interventions was not simply significant. But, no significant differences were identified in pain intensity and unpleasantness ratings. We conclude that there were no differences in the analgesic effects of the four interventions under the present experimental conditions. But, 50 Hz-IFC has been shown to be more comfortable than other interventions.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.11 no.1
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• pp.31-39
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• 2013

Nuclear fuel can be damaged under unexpected circumstances in a nuclear reactor. Fuel rod failure can be occurred due to debris fretting or excessive hydriding or PCI (Pellet-to-clad Interaction) etc. It is important to identify the causes of such failed fuel rods for the safe operation of nuclear power plants. If a fuel rod failure occurs during the operation of a nuclear power plant, the coolant water is contaminated by leaked fission products, and in some case the power level of the plant may be lowered or the operation stopped. In addition, all spent fuels must be transferred to a dry storage. But failed fuel can not be transferred to a dry storage. Therefore, the purpose of this study is to develop a system which is capable of inspecting whether the spent fuel in the storage pool is failed or not. The sipping technology is to analyze the leakage of fission products in state of gas and liquid. The failed fuel inspection system with gamma analyzer has successfully demonstrated that the system is enough to find the failed fuel at Wolsong plant.

• Journal of Energy Engineering
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• v.7 no.2
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• pp.231-240
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• 1998

The global environmental problems such as CFC, energy losses in heat recovery system as well as summer peak time power demands, the development of high efficiency absorption refrigeration systems is one of the most promising method in this problems. The absorption refrigeration system to utilize treated sewage is available for environmental protection and energy conservation. Simulation analysis on the double-effect absorption refrigeration cucles with parallel or series flow type has been performed. LiBr+LiI+LiCl+LiNO$_3$ solution was selected as the new working fluid. The main purpose of this study is evaluating the possibilities of effective utilization of treated sewage as a cooling water for the absorber and condenser. The other purpose of the present study is to determine the optimum designs and operating conditions based on the operating constraints and the coefficient of performance in the parallel or series flow type. In this study, we found out the characteristic of new working solution through the cycle simulation and compared LiBr solution to evaluate. The absorption refrigeration machine using the new working fluid was obtained better results COP rise and compactness of system by comparison with LiBr solution.

• Ocean and Polar Research
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• v.36 no.1
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• pp.25-37
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• 2014

To understand the degradation processes of organic matter related to sulfate reduction by Sulfate Reduction Bacteria (SRB) in the tidal flat sediments of Hwang-do and Sogeun-ri, Tae-an Peninsula in Chungnam-do, biogeochemical characteristics were analyzed and highlighted using specific microbial biomarkers. The organic geochemical parameters (TOC, ${\delta}^{13}C_{org}$, C/N ratio, long-chain-n-alkane) indicate that most of the organic matter has been derived from marine phytoplankton and bacteria in the fine-grained sediment of Sogeun-ri, although terrestrial plant components have occasionally been incorporated to a significant degree in the coarse-grained sediment of Hwang-do. The concentration of sulfate in pore water is a constant tendency with regard to depth profile, while methane concentration appears to be slightly different with regard to depth profile at the two sites. Especially, the sum of bacteria fatty acid (a-C15:0 + i-C15:0 + C16:1w5) confirms that the these concentrations in Sogeun-ri are related to the degradation of Benzene, Toluene, Ethylbenzene and Xylene (BTEX) compounds from the crude oil retained in the sediments as a result of the Hebei Spirit oil-spill accident in 2007. The methane-related microbial communities as shown by lipid biomarkers (crocetane, PMI) are larger in some sedimentary sections of Hwang-do than in the Sogeunri tidal flat. These findings suggest that methane production by microbiological processes is clearly governed by SRB activity along the vertical succession in organic-enriched tidal flats.