• Fisheries and Aquatic Sciences
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• 제4권2호
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• pp.98-100
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• 2001

• 한국패류학회지
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• 제22권2호
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• pp.97-108
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• 2006

Genomic DNA samples isolated from geographical purple Washington clam (Saxidomus purpuratus) were obtained from two different regions in Korean Peninsula: Gunsan (Gunsan population; GSP), and Haeju (Haeju population; HJP), a collection area in the vicinity of the West Sea. The seven arbitrarily primers, OPA-07, OPA-09, OPA-18, OPA-20, OPC-03, OPC-06 and OPC-09 were shown to generate the total loci, loci observed per primer, shared loci by each population, specific, and polymorphic loci which could be clearly scored. We also generated the unique shared loci to each population and shared loci by the two populations in purple Washington clam. The size of the DNA fragments also varied wildly, from 50 to 2,400 bp. Here, 304 total loci were identified in the GSP purple Washington clam population, and 282 in the HJP: 91 polymorphic loci (29.9%) in the GSP and 47 (16.7) in the HJP. 198 shared loci, with an average of 28.3 per primer, were observed in the GSP population. The decamer primer OPA-07 generated the shared loci by the two populations, approximately 1,000 bp, between the two Saxidomus populations. The oligonucleotide primer OPC-03 also generated the shared loci by the two populations, approximately 500 bp and 1,000 bp, in GSP population from Gunsan and HJP population from Haeju. The other primer, OPC-06 generated the shared loci by two Gomphina populations (approximately 400 bp). The dendrogram, generated by seven reliable primers, indicates three genetic clusters. The dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (GUNSAN 01-GUNSAN 02), cluster 2 (GUNSAN 03-GUNSAN 11), and cluster 3 (HAEJU 12-HAEJU 22). The genetic distance between the two geographical populations ranged from 0.043 to 0.506. Especially, the longest genetic distance displaying significant molecular differences, 0.506, was found to exist between individuals GUNSAN no. 11 of Gunsan and HAEJU no. 17 of Haeju.

• 한국수산과학회지
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• 제36권5호
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• pp.495-499
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• 2003

Age and growth of purpulish Washington clam (Saxidomus purpuratus) was investigated from 480 samples monthly collected from January to December 2002 in Jinhae Bay, Korea. Examination of outer margins of the shell revealed that the translucent zone was formed once a year from March to April, it can be used as annulus. Ages were determined from ring radius of shell, and maximum age of the the clam was 9 years. The spawning period was from May to October, and the main spawning occurred in July. The relationship between shell length (SL) and shell height (SH) was $SH=0.8405{\times}SL^{-4.9709}\;(R^2=0.97)$ and that between the shell length (SL) and total weight (TW) was $TW=0.9580{\times}10-4{\times}SL^{3.220}(R^2=0.97).$ The von Bertalanffy growth equation were $SL_t=125.57(1-e^{-0.2523(t+0.5367)},\;TW_t=549.26(1-e^{-0.2523(t+0.5367)})^{3.220}.$

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.259-264
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• 2015

Genomic DNA samples isolated from geographical purplish Washington clam (Saxidomus purpuratus) were obtained from three different regions in the Korean Peninsula: Geoje (Geoje population; GJP), Gunsan (Gunsan population; GSP) and a site of North Korea (North Korea population; NKP). The seven primers generated the total 369 loci that can be scored from the GSP clam population. 356 fragments were generated from the NKP clam population. The complexity of the banding patterns varies dramatically between the primers and three localities. In this study, 319 loci were identified in the purplish Washington clam from Geoje and 369 in the clam population from Gunsan: 221 specific loci (69.3%) in the GJP clam population and 300 (81.3%) in the GSP population. These results demonstrate that the primer detected a large quantity of specific fragments, suggesting that the genetic variation in the GSP is higher than in the GJP population. In particular, the BION-28 primer gave DNA profiles with more fragments than the other six primers in the NKP population. The oligonucleotides primer BION-75 produced 21 unique loci to each population, which were ascertaining each population, approximately 250 bp, 300 bp and 400 bp, in the GJP population. Outstandingly, the primer BION-50 detected 21 shared loci by the three populations, major and/or minor fragments of sizes 150 bp, which were matching in all samples. With regard to average bandsharing value (BS) results, individuals from GJP population (0.743) displayed higher bandsharing values than did individuals from GSP population (0.606). In the present study, the dendrogram gained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (GEOJE 01 ~ GEOJE 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14), cluster 3 (N.KOREA 15 ~ N.KOREA 21). Among the twenty one clams, the shortest genetic distance that revealed significant molecular differences was between individuals 08 and 09 from the NKP population (genetic distance = 0.073), while the longest genetic distance among the twenty-one individuals that demonstrated significant molecular differences was between individuals GEOJE no. 03 and GUNSAN no. 09 (genetic distance = 0.669). Comparatively, individuals of GJP population were properly closely related to that of NKP population, as revealed in the hierarchical dendrogram of genetic distances. In due course, PCR analysis has revealed the significant genetic distance among three purplish Washington clam populations. PCR fragments discovered in this study could be valuable as a DNA marker of the three geographical clam populations to distinguish.

• 한국수산과학회지
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• 제57권1호
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• pp.53-59
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• 2024

The reproductive biology of the Washington clam Saxidomus purpuratus, from Yeosu, Korea was investigated based on 699 individuals collected from January to December 2022. The sex ratio of this species was not significantly different from a 1:1 (male:female) sex ratio (P>0.05). Monthly changes in gonadosomatic index were analyzed to estimate the spawning season. The reproductive cycle of this species can be divided into the following stages: active (Jan.-Mar.), ripe (Mar.-Nov.), spent (Apr.-Dec.), and degenerative (Jun.-Dec.) stages. The spawning period ranged from March to December with the main periods being from April to June and from September to November. The shell length at 50% female group maturity (SL₅₀) was estimated to be 60.2 mm.

• 한국패류학회지
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• 제23권1호
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• pp.31-38
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• 2007

본 연구에서는 남해안 개조개의 자원생태학적 특성치인 생잔율 (S = 0.5174), 순간자연사망계수 (M = 0.2012/year) 및 순간어획사망계수 (F = 0.4578/year), 어획개시연령 ($t_c\;＝\;2.7506$ 세), 자원량 (B = 8.362 톤) 을 추정하였다. 갯장어 자원을 가입당생산량모델에 적용시킨 결과, 현재의 순간어획사망계수 (F = 0.4578/year), 어획개시연령 ($t_c\;＝\;2.7506$ 세) 에서의 가입당생산량은 80.89 g으로 추정되었다. 따라서 현재의 순간어획사망계수를 그대로 유지한다면 어획개시연령을 4 세 이상으로 높여야 하고, 현재의 어획개시연령을 유지한다면 순간어획사망계수를 0.2 보다 낮은 수준으로 낮추어야한다. $F_{0.1}$의 경우, 2 세에서 65.69 g으로 가장 높은 가입당생산량을 나타내고 있다. 또한 가입당산란자원량모델을 이용하여 생물학적 관리기준인 $F_{40%}$의 값을 현재의 어획상태를 고려하여 추정하였다.

• 한국양식학회지
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• 제17권4호
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• pp.251-257
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• 2004

상업적 가치가 높고 양식 가능성이 있는 백합과 5종을 대상으로 RAPD방법을 이용한 개체간의 유전적 유연관계를 조사하였다. DNA 추출은 protein K-phenol방법을 사용하여 각 종의 후폐각근에서 추출하였다. RAPD-PCR결과 15개의 primer들이 증폭되었고, 그들로부터 각각 1-3개의 band를 볼 수 있었다. 백합과 5종간 유전적 유사도는 살조개와 바지락간에는 0.84, 개조개와 백합간에는 0.87로 각각의 두 종간에는 놓은 유사도를 보였고, 가무락조개는 살조개와 바지락간에 0.78의 유사도를 보였으며, 개조개와 백합, 살조개, 바지락, 가무락조개간에는 0.46의 비교적 낮은 유전적 유사도를 보였다. RAPD 방법으로 종내개체 유전변이를 파악하기는 힘들어도, 양식이나 자원증식을 위한 패류의 우수형질 선택에 있어 기본 정보를 제공 할 수는 있을 것이다. 또한, 살조개 대량종묘생산의 방법은 유전적 유사도가 가장 가까운 바지락을 중심으로 이루어져야 할 것으로 사료된다.

• 한국식품영양과학회지
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• 제30권1호
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• pp.43-47
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• 2001

Electron spin resonance (ESR) spectroscopy was used to investigate the effect of irradiation dose on the ESR signal intensity and to identify the stability of radicals after 9 weeks of storage in order to detect irradiated shellfishes. The irradiated shellfishes (short-necked clam, purplish washington clam, freshwater clam, jackknifed clam, scallop and hard-shell mussel) presented an asymmetric absorption in shape at $g_{1}$=2.002~2.003 and $g_{2}$=1.998. The strength of the ESR signal increased linearly with the applied doses (1~7 kGy). A highly positive correlation coefficients ($R^{2}$=0.9136~0.9896) were obtained between the irradiation dose and corresponding ESR signal intensity. The intensity of the signals after irradiation was stable even after 9 weeks of storage at 5$\pm$1$^{\circ}C$.

• 한국패류학회지
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• 제23권2호
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• pp.165-172
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• 2007

우리나라 남해안 강진만에서 2006년 5월부터 2007년 4월까지 채집된 개조개의 생식소 발달과 생식주기를 조직학적으로 조사하였다. 개조개는 자웅이체로 난소는 수많은 난소소낭 (oogenic follicle) 으로 이루어져 있으며, 정소는 다수의 정소 소낭 (acinus) 으로 구성되어 있다. 생식소지수는 4월에 최고값을 나타내었고, 그 후 감소되어 7월에 최저값를 나타내었다. 비만도지수는 6월에 최고값을 보였고, 그 후 감소되어 11월에 최저값을 보였다. 개조개의 생식주기는 회복 및 휴지기 (6-12월), 초기활성기 (1-4월), 후기활성기 (2-7월), 완숙기 (3-10월), 방출기 (4-10월)로 구분되었다. 강진만에서 개조개의 주산란기는 7-9월이었다. 암, 수 성비는 1:1로 유의한 차를 보이지 않았다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.250-251
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• 2003

Biochemical and biometric features of eggs of the Washington clam, Saxidomus purpuratus, are reported in this study. In addition, a polyclonal antibody against the purified clam egg was employed to quantitative assessment of eggs of the clams collected from Geoje Island, Korea in May 2002. Mean egg diameter was 70.81 $\pm$ 7.52 ${\mu}{\textrm}{m}$(histological preparation) or 88.56 $\pm$ 11.31 ${\mu}{\textrm}{m}$(purified oocytes), and individual egg weighed 95 ng in dry weigt. (omitted)