• Proceedings of the Plant Resources Society of Korea Conference
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• 1995.01a
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• pp.34-34
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• 1995

• Korean Journal of Medicinal Crop Science
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• v.12 no.4
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• pp.300-303
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• 2004

This experiment was conducted to study the effect of growth characteristics and yield by different planting density on Wasabia japonica Matsum. in water culture. Plant height, leaf length, petiole length was long in spacious planting $(30{\times}25\;cm)$ and short in dense planting$(30{\times}15\;cm)$. Rhizome width was thick in spacious planting, and was thin in dense planting. Rhizome weight per plant decreased by increasing planting density, but rhizome yield was increased by high planting density. But distribution of rhizome weight did not show significant difference at different planting density.

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.64-67
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• 1996

This experiment was carried out to obtain the information on seed dormancy, germinability during storage in wasabia japonica Matsum. $GA_3$ 100ppm+BA 10ppm was more effective than $GA_3$100ppm alone in breaking the dormancy. Storage of seeds pretreated with growth retardants (CCC, Uniconazol) seemed to be a useful method for preventing germination during storage. The stratification was an effective storage method to germinate the seeds for sowing them in fall.

• Nutrition Research and Practice
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• v.7 no.4
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• pp.267-272
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• 2013

The anti-obesity effects of a hot water extract from wasabi (Wasabia japonica Matsum.) leaves (WLE), without its specific pungent constituents, such as allyl-isothiocyanate, were investigated in high fat-diet induced mice. C57J/BL mice were fed a high-fat diet (control group) or a high-fat diet supplemented with 5% WLE (WLE group). Physical parameters and blood profiles were determined. Gene expression associated with lipid metabolism in liver and white adipose tissue were analyzed. After 120 days of feeding, significantly lower body weight gain, liver weight and epididymal white adipose tissue weight was observed in the WLE group compared to the control group. In liver gene expression within the WLE group, PPAR${\alpha}$ was significantly enhanced and SREBP-1c was significantly suppressed. Subsequent downstream genes controlled by these regulators were significantly suppressed. In epididymal white adipose tissue of the WLE group, expression of leptin, PPAR${\gamma}$, and C/EBP${\alpha}$ were significantly suppressed and adiponectin was significantly enhanced. Acox, related to fatty acid oxidization in adipocytes, was also enhanced. Our results demonstrate that the WLE dietary supplement induces mild suppression of obesity in a high-fat diet induced mice, possibly due to suppression of lipid accumulation in liver and white adipose tissue.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.473-477
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• 2004

Wasabis (Wasabia japonica Matsum.) have been used as a spice with its petioles and rhizomes, but the production of its rhizomes in upland cultivation was very difficult for the severe damage of rotting disease during summer season. This experiment was carried out to increase the yield of marketable rhizome in wasabi when cultured in upland. Seedlings were raised in sub-alpine area for 7 and 12 months and then transplanted to experimental area on Sept. 20 and the yield was investigated on May 12 of the next year. The marketable rhizome(above 40g/plant) was produced in seedlings raised for 7 months. Production rate of marketable rhizome was 13.3% and total yield was 72.9 kg/10a. The content of allylisothiocyanate in rhizome of upland wasabi was 0.777 mg/g and its content was equal to wasabi cultured in water condition for 18 months. These results suggested that the upland cultivation of wasabi seedlings raised in sub-alpine area for 7 months was possible to product marketable rhizome.

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.12-18
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• 1996

This study was investigated to improve the rate of the seed germination of Wasabia japonica with $GA_3$ and BAP. The germination rate was 36% with 100ppm $GA_3$ only and 32% with 10ppm BAP only, while that was 65% with combination of 100ppm $GA_3$ and 10ppm BAP compared to that of 7.5% in control. This results show us that the most effect of germination rate was with 100ppm $GA_3$ and 10ppm BAP. Mutagenesis was induced with mutagen, EMS and colchicine. Before the germination, the survival rate was 62% with 0.5% colchicine compared to that of 7% in control. After the germination, the 85% of developed seed was alive with treatment of 0.5% colchicine and 55% of those was alive with 0.06% EMS compared to that of 8% in control. We analyzed the karyotype and isozyme pattern of Wasabia japonica induced with colchicine and EMS. The tetratype of Wasabia japonica was observed from Wasabia japonica treated with 0.5% colchicine. whereas it was not observed with 0.06% EMS. The peroxidase pattern of colchicine treated Wasabia japonica was different from nontreatment. but that of EMS treated Wasabia japonica was the same with normal one.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.180-183
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• 2003

P. wasabiae was isolated from discolored seeds of wasabi(Wasabia japonical Mtsum.) and inoculated to fresh seeds, then the effect of fungicides on suppression of diseases were determined. Emergence rate of wasabi seeds where suppressed to 52.5% by the inoculation and it reached up to 92.7% by dipping treatment of inoculated seeds ito benomyl solution. The incidence rate of black leg disease and damping off were 32.0 and 22.0%, respectively, in control treatment that sown in the soil inoculated with P. wasabiae. But dipping treatment of inoculated seeds into benomyl solution resulted in 12.0% and 10.7% in incidence rate of those two diseases, respectively.

• Korean Journal of Environment and Ecology
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• v.22 no.5
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• pp.530-535
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• 2008

This study was carried out to analyze the vegetation and soil characteristic, and ordination of Wasabia japonica population at Ulleung-Do. The Wasabia japonica population is distributed in altitudes of 440m to 570m at Seo-Myon and also in altitudes of 340m near Nari-valley. In the study sites, soil organic matter, nitrogen, available phosphorous, exchangeable potassium, exchangeable calcium, exchangeable magnesium concentration, cation exchange capacity, and soil pH were 15.01%, 0.48%, 44.19ppm, 2.00(me/100g), 7.19(me/100g), 4.44(me/100g), 22.55(me/100g), and 6.17, respectively. The Rumohra standishii dominant population was mainly found in high in soil nitrogen, organic matter, CEC, and exchangeable calcium concentration in comparison with Boehmeria spicata dominant population. The Boehmeria spicata dominant population was mainly found in high in soil pH.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.31-35
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• 2004

The genetic variation and intraspecific relationships between 10 individuals of seven cultivars and one Ulleungdo native of Wasabia japonica were investigated using RAPD (Randomly Amplified Polymorphic DNA) analysis. The 21 primers out of 50 random primers were amplified for all tested plants. The 68 (47.2%) among 144 bands derived from 21 primers showed polymorphism, and 3.2 bands per primer were observed. Number of bands per primer was ranged from 2 to 13, and average numbers were 6.8. The phenograms for 11 analyzed individuals by RAPD markers were not matched well with those of the result by morphological characters since they were clustered monophyletic at the similarity coefficient value ranged from 0.81 to 0.96. The Ulleungdo native individual was clustered sister to Daruma, Simanesairal, Sawa, and Hujidaruma cultivars. The RAPD markers were not useful to evaluate the intraspecific variations in Wasabia japonica cultivars, therefore need to more specific molecular phylogenetic characters such as AFLP technology and gene sequence of nuclear and chloroplast DNA.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.8
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• pp.1057-1063
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• 2006

The Dongchimi added with Wasabi (Wasabia japonica Matsum) for the improvement of quality was scientifically explored by reviewing the optimum level and its effect on the microbiological and sensory characteristics of product fermentation. The final weight percentage of Wasabi in Dongchimi was adjusted to 0, 3, 5, 7 and 9%, per radish, respectively. Microbiological and sensory characteristics were determined during fermentation at $10^{\circ}C$ for 55 days. The viable cell counts of total and lactic acid bacteria had increased to their maximum values during fermentation until they began to drop at the later stage. Here, the 7% treatment showed a distinctive low and high numbers of total cells and lactic acid bacteria, respectively, during the later stage of fermentation. As for the sensory evaluation results of the fermented Donchimi with Wasabi, the 7% treatment was most favored for the color, the smell, the sour taste, the hot taste, the carbonated taste, the texture, and the overall acceptability of the products. The optimum levels of Wasabi in Dongchimi were estimated between 5 and 7% per added radish weight, and the 7% level was more preferred when the fermentation-retarding effect of the product was considered.