• Journal of Korean Medical classics
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• v.25 no.2
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• pp.25-41
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• 2012

Objective : Neijingshiyifanglun with additions and emendations was written by Liu Yude, a doctor who lived during Ming period. I researched the origin of the book, and analyzed the features of it as well. I also approximated his birth date and death date. In doing this, I gained a better understanding the practice of medicine in ancient China. Method : I researched the book by comparing its contents, including the causes of diseases, the descriptions of symptoms, the transmissions of diseases, and treatments, with other sources that he had referenced. Result : In understanding Hwangdineijing, Liu Yude was influenced by many medical scholars such as, Wang Bing, Ma Shi, and Wu Kun, but his opinion is most similar to that of Zhang Jiebin. In the field of the Chinese Medical Theory, he was deeply influenced by 'JinYuan-Sidaijia's theories, particularly Li Gao and Zhu Zhenheng. In fanglun, he was greatly influenced by Yifangkao. He concluded that 'aggregationaccumulation' was a disease of stuffiness, and suggested its cure in through 'yangjingzezichu' and 'treatment of blood aspect'. He recognized the disease of 'reversal of qi' as the disease of 'jiaoqi'. He also indicated that the word of 'qi' is not 'rough' but 'tears' or 'yingfengliulei'. Conclusion : 1. He was an excellent medical practitioner and scholar in the history of oriental medicine. 2. He found and corrected errors in the opinions of Wang Bing, Ma Shi, and Wu Kun. 3. He frequently practiced Taipinghuiminhejijufang, and considered Spleen-Stomach, yin-blood, and fire-heat important. 4. He captured the spirit of Huangdisuwenxuanminglunfang, Neijingshiyifanglun, Yifangkao in views of remedy and theory. 5. Neijingshiyifanglun with additions and emendations is the most comprehensive book about fanglun because of its thorough analysis of the Hwangdineijing and its connection to the treatment of ancient diseases in Oriental Medical History.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4301-4306
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• 2013

Aim: Apoptosis has been considered as a fundamental component in cancer pathogenesis, and related genetic factors might play an important role in gastric cardiac adenocarcinoma (GCA) genesis. Methods: We conducted a hospital based case.control study to evaluate the genetic effects of functional single nucleotide polymorphisms (SNPs): BCL2 rs17757541 C>G, BCL2 rs12454712 T>C, FAS rs2234767 G>A, FASL/FASLG rs763110 C>T, ERBB2 rs1136201 A>G and VEGFR2/KDR rs11941492 C>T on the development of GCA. A total of 243 GCA cases and 476 controls were recruited for the study and genotypes were determined using a custom-by-design 48-Plex SNPscan$^{TM}$ Kit. Results: The BCL2 rs17757541 C>G polymorphism was associated with increased risk of GCA. However, there was no significant associations with the other five SNPs. Stratified analyses indicated a significantly increased risk of GCA associated with the BCL2 rs17757541 C>G polymorphism among males, older patients and those with a history of smoking or drinking. Conclusion: These findings indicated that the functional polymorphism BCL2 rs17757541 C>G might contribute to GCA susceptibility. However, our results were limited by small sample size. Future larger studies are required to confirm our current findings.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5409-5413
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• 2012

At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4453-4458
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• 2012

Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.

• Journal of Power Electronics
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• v.17 no.2
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• pp.432-441
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• 2017

Grid voltage feedforward is extensively used for controlling grid-connected converters. However, the conventional voltage feedforward control reduces the stability margins of the converter connected to a high-impedance grid. The effect mechanism of voltage feedforward on the grid-connected converter control under high-inductive conditions of the grid impedance is clearly explained in this study using the equivalent transformations of control block diagrams. Results show that the delay produced by the digital control is the root cause of this effect. An improved voltage feedforward strategy, in which a bandpass filter (BPF) is introduced into the feedforward path, is proposed to strengthen the converter's robust stability against grid impedance variations. The selection method of the BPF's bandwidth is also provided considering the tradeoff between the response speed to the grid voltage sag and the system's robust stability. The converter can work stably over a wide range of the grid impedance through the proposed approach. Simulation and experimental results fully verify the effectiveness of the BPF-based voltage feedforward strategy.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2911-2916
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• 2014

Oxaliplatin is a first-line therapy for colorectal cancer, but cancer cell resistance to the drug compromises its efficacy. To explore mechanisms of drug resistance, we treated colorectal cancer cells (HCT116 and SW620) long-term with oxaliplatin and established stable oxaliplatin-resistant lines (HCT116-OX and SW620-OX). Compared with parental cell lines, $IC_{50}$s for various chemotherapeutic agents (oxaliplatin, cisplatin and doxorubicin) were increased in oxaliplatin-resistant cell lines and this was accompanied by activation of nuclear factor erythroid-2 p45-related factor 2 (Nrf2) and NADPH quinone oxidoreductase 1 (NQO1). Furthermore, luteolin inhibited the Nrf2 pathway in oxaliplatin-resistant cell lines in a dose-dependent manner. Luteolin also inhibited Nrf2 target gene [NQO1, heme oxygenase-1 (HO-1) and $GST{\alpha}1/2$] expression and decreased reduced glutathione in wild type mouse small intestinal cells. There was no apparent effect in Nrf2-/- mice. Luteolin combined with other chemotherapeutics had greater anti-cancer activity in resistant cell lines (combined index values below 1), indicating a synergistic effect. Therefore, adaptive activation of Nrf2 may contribute to the development of acquired drug-resistance and luteolin could restore sensitivity of oxaliplatin-resistant cell lines to chemotherapeutic drugs. Inhibition of the Nrf2 pathway may be the mechanism for this restored therapeutic response.

• Journal of Electrochemical Science and Technology
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• v.11 no.4
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• pp.368-378
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• 2020

In the paper, corrosion behavior of T91 steel in different concentrations of NaHSO₃ solution was studied in combination with scanning electron microscope (SEM) and electrochemical measurements. The results showed that the steel exhibited active anodic dissolution characteristics in the solution, and NaHSO₃ concentration affected both cathodic and anodic behaviors. The steel surface was covered by intact corrosion products in the solutions, but the compactness and mechanical properties of the corrosion products degraded with the increase of NaHSO₃ concentration. In low-concentration NaHSO₃ solution the steel tended to undergo uniform corrosion with slight corrosion pits, but its corrosion mode gradually transited to localized corrosion as the NaHSO₃ concentration increased. The mechanical property degradation of the corrosion products caused by sulfur compounds and the pH decrease of the solution are the important factors to accelerating its corrosion process.

• Molecules and Cells
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• v.37 no.12
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• pp.857-864
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• 2014

Astrocyte elevated gene-1 (AEG-1) is a recently discovered oncogene that has been reported to be highly expressed in various types of malignant tumors, including renal cell carcinoma. However, the precise role of AEG-1 in renal cancer cell proliferation and apoptosis has not been clarified. In this study, we transfected the renal cancer cell line Caki-1 with a plasmid expressing AEG-1 short hairpin RNA (shRNA) and obtained cell colonies with stable knockdown of AEG-1. We found that AEG-1 down-regulation inhibited cell proliferation and colony formation and arrested cell cycle progression at the sub-G1 and G0/G1 phase. Western blot analysis indicated that the expression of proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E were significantly reduced following AEG-1 down-regulation. In addition, AEG-1 knockdown led to the appearance of apoptotic bodies in renal cancer cells, and the ratio of apoptotic cells significantly increased. Expression of the antiapoptotic factor Bcl-2 was dramatically reduced, whereas the pro-apoptotic factors Bax, caspase-3 and poly (ADPribose) polymerase (PARP) were significantly activated. Finally, AEG-1 knockdown in Caki-1 cells remarkably suppressed cell proliferation and enhanced cell apoptosis in response to 5-fluorouracil (5-FU) treatment, suggesting that AEG-1 inhibition sensitizes Caki-1 cells to 5-FU. Taken together, our data suggest that AEG-1 plays an important role in renal cancer formation and development and may be a potential target for future gene therapy for renal cell carcinoma.

• BMB Reports
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• v.37 no.4
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• pp.402-407
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• 2004

A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.

• Steel and Composite Structures
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• v.23 no.6
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• pp.727-736
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• 2017

The application of carbon fiber reinforced polymer (CFRP) in steel structures primarily includes two categories, i.e., the bond-critical application and the contact-critical application. Debonding failure and buckling failure are the main failure modes for these two applications. Conventional electrometric techniques may not provide precise results because of the limitations associated with single-point contact measurements. A nondestructive full-field measurement technique is a valuable alternative to conventional methods. In this study, the digital image correlation (DIC) technique was adopted to investigate the bond behavior and buckling behavior of CFRP-steel composite members. The CFRP-to-steel bonded joint and the CFRP-strengthened square hollow section (SHS) steel column were tested to verify the suitability of the DIC technique. The stereo-DIC technique was utilized to measure continuous deformation. The bond-slip relationship of the CFRP-to-steel interface was derived using the DIC data. Additionally, a multi-camera DIC system consisting of four stereo-DIC subsystems was proposed and applied to the compressive test of CFRP-strengthened SHS steel column. The precise buckling location and CFRP delamination of the CFRP-strengthened SHS steel column were identified. The experimental results confirm that the stereo-DIC technique can provide effective measurements for investigating the behaviors of CFRP-steel composite members.