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http://dx.doi.org/10.7314/APJCP.2012.13.9.4453

Tanshinone II-A Inhibits Angiogenesis through Down Regulation of COX-2 in Human Colorectal Cancer  

Zhou, Li-Hong (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Hu, Qiang (Department of Genernal Surgery, Dahua Hospital)
Sui, Hua (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Ci, Shu-Jun (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Wang, Yan (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Liu, Xuan (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Liu, Ning-Ning (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Yin, Pei-Hao (Department of Genernal Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine)
Qin, Jian-Min (Department of Genernal Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine)
Li, Qi (Clinic Oncology, Putuo Hospital & Cancer Institute, Shanghai University of Traditional Chinese Medicine)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.9, 2012 , pp. 4453-4458 More about this Journal
Abstract
Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.
Keywords
Tanshinone II-A; colorectal carcinoma; angiogenesis; cyclooxygenase 2; vascular endothelial growth factor;
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